| staphylococcus agnetis sp. nov., a coagulase-variable species from bovine subclinical and mild clinical mastitis. | thirteen gram-positive coagulase-variable staphylococci were isolated from subclinical and mild clinical mastitic bovine milk (n = 12) and a teat apex (n = 1). the results of sequence analysis of the 16s rrna gene and two housekeeping genes, rpob and tuf, and dna fingerprinting with amplified fragment length polymorphism (aflp) analysis show that the isolates form a separate branch within the genus staphylococcus. the phylogenetically most closely related species are s. hyicus and s. chromogenes ... | 2011 | 21335502 |
| molecular detection and sensitivity to antibiotics and bacteriocins of pathogens isolated from bovine mastitis in family dairy herds of central mexico. | thirty-two farms (n = 535 cows) located in the state of guanajuato, mexico, were sampled. pathogens from bovine subclinical mastitis (scm) and clinical mastitis (clm) were identified by 16s rdna and the sensitivity to both antibiotics and bacteriocins of bacillus thuringiensis was tested. forty-six milk samples were selected for their positive california mastitis test (cmt) (≥3) and any abnormality in the udder or milk. the frequency of scm and clm was 39.1% and 9.3%, respectively. averages for ... | 2015 | 25815326 |
| short communication: prevalence of methicillin resistance in coagulase-negative staphylococci and staphylococcus aureus isolated from bulk milk on organic and conventional dairy farms in the united states. | the objective of this study was to evaluate the presence of methicillin-resistant staphylococcus aureus and coagulase-negative staphylococcus spp. in bulk tank milk samples from 288 organic and conventional dairy farms located in new york, wisconsin, and oregon from march 2009 to may 2011. due to recent publications reporting the presence mecc (a meca homolog not detected by traditional meca-based pcr methods), a combination of genotypic and phenotypic approaches was used to enhance the recovery ... | 2014 | 24582450 |
| speciation and strain-typing of staphylococcus agnetis and staphylococcus hyicus isolated from bovine milk using a novel multiplex pcr and pulsed-field gel electrophoresis. | staphylococcus hyicus and staphylococcus agnetis are two coagulase variable staphylococcal species that can be isolated from bovine milk and are difficult to differentiate. the objectives of this study were to characterize isolates of bovine milk origin from a collection that had previously been characterized as coagulase positive s. hyicus based on phenotypic speciation methods and to develop a pcr-based method for differentiating s. hyicus, s. agnetis, and s. aureus. isolates (n = 62) were sel ... | 2017 | 28330895 |
| chondronecrosis with osteomyelitis in broilers: further defining a bacterial challenge model using the wire flooring model. | lameness in broiler chickens is a significant animal welfare and financial issue. bacterial chondronecrosis with osteomyelitis (bco) leading to lameness can be enhanced by rearing young broilers on wire flooring. using the wire floor system, we identified staphylococcus agnetis as the predominant isolate in bco of the proximal tibiae and femora, and blood of lame broilers. administration of s. agnetis isolates in water can induce lameness. we now report that the wire floor system increases bacte ... | 2017 | 27587724 |
| genome analysis of staphylococcus agnetis, an agent of lameness in broiler chickens. | lameness in broiler chickens is a significant animal welfare and financial issue. lameness can be enhanced by rearing young broilers on wire flooring. we have identified staphylococcus agnetis as significantly involved in bacterial chondronecrosis with osteomyelitis (bco) in proximal tibia and femorae, leading to lameness in broiler chickens in the wire floor system. administration of s. agnetis in water induces lameness. previously reported in some cases of cattle mastitis, this is the first re ... | 2015 | 26606420 |
| draft genome sequence of bovine mastitis isolate staphylococcus agnetis cbmrn 20813338. | presented here is a draft genome sequence for staphylococcus agnetis cbmrn 20813338, isolated from a lactating dairy cow with subclinical mastitis. the genome is approximately 2,416 kb and has 35.79% g+c content. analysis of the deduced open reading frame (orf) set identified candidate virulence attributes in addition to potential molecular targets for species identification. | 2014 | 25189590 |
| bovine-associated cns species resist phagocytosis differently. | coagulase-negative staphylococci (cns) cause usually subclinical or mild clinical bovine mastitis, which often remains persistent. symptoms are usually mild, mostly only comprising slight changes in the appearance of milk and possibly slight swelling. however, clinical mastitis with severe signs has also been reported. the reasons for the differences in clinical expression are largely unknown. macrophages play an important role in the innate immunity of the udder. this study examined phagocytosi ... | 2013 | 24207012 |
| species level identification of coagulase negative staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization-time of flight mass spectrometry and cydb real-time quantitative pcr. | incorrect identification of staphylococcus spp. can have serious clinical and zoonotic repercussions. accordingly, the aim of this study was to determine if matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) and/or cydb real- time quantitative pcr (qpcr) could be used to accurately identify coagulase negative staphylococcus spp. (cons) obtained from buffalo milk and milking environment samples. seventy-five of 84 cons isolates could be identified to the s ... | 2017 | 28532810 |
| distribution of non-aureus staphylococci species in udder quarters with low and high somatic cell count, and clinical mastitis. | the effect of non-aureus staphylococci (nas) in bovine mammary health is controversial. overall, nas intramammary infections (imi) increase somatic cell count (scc), with an effect categorized as mild, mostly causing subclinical or mild to moderate clinical mastitis. however, based on recent studies, specific nas may affect the udder more severely. some of these apparent discrepancies could be attributed to the large number of species that compose the nas group. the objectives of this study were ... | 2017 | 28456402 |