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revised phylogeny of rhizobiaceae: proposal of the delineation of pararhizobium gen. nov., and 13 new species combinations.the family rhizobiaceae accommodates the seven genera rhizobium, neorhizobium, allorhizobium, agrobacterium, ensifer (syn. sinorhizobium), shinella and ciceribacter. however, several so-called rhizobium species do not exhibit robust phylogenetic positions. rhizobium is extremely heterogeneous and is in need of major revision. therefore, a phylogenetic examination of the family rhizobiaceae by multilocus sequence analysis (mlsa) of four housekeeping genes among 100 strains of the family was under ...201525595870
single acquisition of protelomerase gave rise to speciation of a large and diverse clade within the agrobacterium/rhizobium supercluster characterized by the presence of a linear chromid.linear chromosomes are atypical in bacteria and likely a secondary trait derived from ancestral circular molecules. within the rhizobiaceae family, whose genome contains at least two chromosomes, a particularity of agrobacterium fabrum (formerly a. tumefaciens) secondary chromosome (chromid) is to be linear and hairpin-ended thanks to the tela protelomerase. linear topology and tela distributions within this bacterial family was screened by pulse field gel electrophoresis and pcr. in a. rubi, a. ...201424440816
rhizobium skierniewicense sp. nov. isolated from tumors on chrysanthemum and prunus in poland.three gram-negative, rod-shaped, non-spore-forming bacteria were isolated from galls on chrysanthemum (ch11(t), ch12) and on prunus cerasifera var. divaricata (al 9.3). all three isolates are able to cause crown gall on different plant species. on the basis of 16s rrna gene sequence similarity, the strains belong to the genus rhizobium and their closest relatives are rhizobium rubi (99.6%), rhizobium radiobacter (98.7%) and rhizobium larrymoorei (98.1%). phylogenetic analysis of the novel strain ...201121642489
agrobacterium bohemicum sp. nov. isolated from poppy seed wastes in central bohemia.two non-pathogenic strains r89-1 and r90t isolated from poppy seed (papaver somniferum l.) wastes were phenotypically and genotypically characterized. multilocus sequence analysis (mlsa) was conducted with six genes (atpd, glna, gyrb, reca, rpob, 16s rrna). the strains represented a new species which clustered with agrobacterium rubi nbrc 13261t and agrobacterium skierniewicense ch11t type strains. mlsa was further accompanied by whole-genome phylogeny, in silico dna-dna hybridization (dddh) and ...201829402492
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