| isolation of uracil auxotrophs from burkholderia cepacia atcc 25416. | two uracil auxotrophs of the phytopathogen burkolderia cepacia atcc 25416, which is known to be involved in food spoilage, were isolated by a combination of ethylmethane sulphonate and d-cycloserine counterselection. one mutant exhibited depressed orotate phosphoribosyltransferase activity while the other mutant lacked orotidine 5'-monophosphate decarboxylase activity. pyrimidine limitation of either auxotroph elevated aspartate transcarbamoylase and dihydroorotase activities by at least 1:5-fol ... | 1995 | 7576529 |
| pyrimidine synthesis in burkholderia cepacia atcc 25416. | pyrimidine synthesis in the food spoilage agent burkholderia cepacia atcc 25416 was investigated. the five de novo pathway enzymes of pyrimidine biosynthesis were found to be active in b. cepacia atcc 25416 and growth of this strain on uracil had an effect on the de novo enzyme activities. the in vitro regulation of aspartate transcarbamoylase activity in b. cepacia atcc 25416 was studies and its activity was inhibited by pp(i), atp, gtp, ctp and utp. the enzymes cytidine deaminase, uridine phos ... | 1995 | 7576530 |
| role of cytosine deaminase and beta-alanine-pyruvate transaminase in pyrimidine base catabolism by burkholderia cepacia. | a determination of the possible role of the salvage enzyme cytosine deaminase or beta-alanine-pyruvate transaminase in the catabolism of the pyrimidine bases uracil and thymine by the opportunistic pathogen burkholderia cepacia atcc 25416 was undertaken. it was of interest to learn whether these enzymes were influenced by cell growth on pyrimidine bases and their respective catabolic products to the same degree as the pyrimidine reductive catabolic enzymes were. it was found that cytosine deamin ... | 2000 | 10696871 |
| construction of a deep-rough mutant of burkholderia cepacia atcc 25416 and characterization of its chemical and biological properties. | burkholderia cepacia is a bacterium with increasing importance as a pathogen in patients with cystic fibrosis. the deep-rough mutant ko2b was generated from b. cepacia type strain atcc 25416 by insertion of a kanamycin resistance cassette into the gene waac encoding heptosyltransferase i. mass spectrometric analysis of the de-o-acylated lipopolysaccharide (lps) of the mutant showed that it consisted of a bisphosphorylated glucosamine backbone with two 3-hydroxyhexadecanoic acids in amide-linkage ... | 2003 | 12427755 |
| identification of an rtx determinant of burkholderia cenocepacia j2315 by subtractive hybridization. | this study utilized suppressive subtractive hybridization between the clinical isolate burkholderia cenocepacia j2315 and the closely related environmental isolate burkholderia cepacia atcc 25416(t) to isolate dna fragments specific to b. cenocepacia j2315. analysis of the resulting pools of b. cenocepacia-specific dnas identified several fragments that may be part of putative virulence factors. further in silico analysis of a single fragment indicated that it was internal to a gene of which the ... | 2006 | 16388025 |
| isolation, heterologous expression and characterization of an endo-polygalacturonase produced by the phytopathogen burkholderia cepacia. | endo-polygalacturonases (endopgs) belong to the glycoside hydrolase family 28 and hydrolyze the alpha-1,4 glycosidic bond present in the smooth regions of pectins. pectic substances are among the principal macromolecular components of the primary plant cell walls and are subjected to enzymatic degradation not only in the course of important physiological processes such as plant senescence and ripening, but also during infection events by plant pathogens. here we report, for the first time, the i ... | 2007 | 17493828 |
| influence of legionella pneumophila and other water bacteria on the survival and growth of acanthamoeba polyphaga. | we investigated in solid medium, in water microcosm co-cultures and by light and transmission electron microscopy the influence of legionella pneumophila lp-1, pseudomonas aeruginosa atcc 27853, burkholderia cepacia atcc 25416 and pseudomonas fluorescens ssd35 on the growth and survival of acanthamoeba polyphaga. the infection with l. pneumophila was microscopically characterized by the presence of few bacteria inside protozoa at 4th h, and by the beginning of disruptive effects in late phase of ... | 2010 | 20730523 |