| gordonia neofelifaecis sp. nov., a cholesterol side-chain-cleaving actinomycete isolated from the faeces of neofelis nebulosa. | a cholesterol side-chain-cleaving bacterial strain, ad-6(t), was isolated from fresh faeces of a clouded leopard (neofelis nebulosa) and was studied using a polyphasic taxonomic approach. 16s rrna gene sequence analysis showed that the novel strain formed a distinct subline within the genus gordonia, its closest neighbours being the type strains of gordonia cholesterolivorans, gordonia sihwensis and gordonia hydrophobica, with sequence similarity values of 98.2, 97.8 and 97.6 %, respectively. th ... | 2011 | 20190025 |
| draft genome sequence of gordonia neofelifaecis nrrl b-59395, a cholesterol-degrading actinomycete. | we report a draft sequence of the genome of gordonia neofelifaecis nrrl b-59395, a cholesterol-degrading actinomycete isolated from fresh feces of a clouded leopard (neofelis nebulosa). as predicted, the reported genome contains several gene clusters for cholesterol degradation. this is the second available genome sequence of the family gordoniaceae. | 2011 | 21742880 |
| heterologous expression and characterization of a 3-ketosteroid-∆(1)-dehydrogenase from gordonia neofelifaecis and its utilization in the bioconversion of androst-4,9(11)-dien-3,17-dione. | 3-ketosteroid-∆(1)-dehydrogenase (kstd), a key enzyme in microbial steroid catabolism, catalyzes the trans-axial elimination of the c1 and c2 hydrogen atoms of the a-ring from the polycyclic ring structure of 3-ketosteroids, and it was usually used to transform androst-4-ene-3,17-dione (ad) to produce androsta-1,4-diene-3,17-dione. here, the kstd from gordonia neofelifaecis was expressed efficiently in escherichia coli. e. coli cells expressing kstd3gor were subjected to the investigation of deh ... | 2017 | 28391482 |
| complete genome sequences of 38 gordonia sp. bacteriophages. | we report here the genome sequences of 38 newly isolated bacteriophages using gordonia terrae 3612 (atcc 25594) and gordonia neofelifaecis nrrl59395 as bacterial hosts. all of the phages are double-stranded dna (dsdna) tail phages with siphoviral morphologies, with genome sizes ranging from 17,118 bp to 93,843 bp and spanning considerable nucleotide sequence diversity. | 2017 | 28057748 |
| [accumulation of 9α-hydroxy-4-androstene-3,17-dione by co-expressing ksha and kshb encoding component of 3-ketosteroid-9α-hydroxylase in mycobacterium sp. nrrl b-3805]. | 9α-hydroxy-4-androstene-3,17-dione (9-oh-ad) is an important intermediate in the steroidal drugs production. 3-ketosteroid-9α-hydroxylase (ksh), a two protein system of ksha and kshb, is a key-enzyme in the microbial steroid ring b-opening pathway. ksh catalyzes the transformation of 4-androstene-3,17-dione (ad) into 9-oh-ad specifically. in the present study, the putative ksha and kshb genes were cloned from mycobacterium smegmatis mc(2)155 and gordonia neofelifaecis nrrl b-59395 respectively, ... | 2015 | 26380409 |
| identification of gene expression profiles in the actinomycete gordonia neofelifaecis grown with different steroids. | gordonia neofelifaecis nrrl b-59395 was initially isolated from the fresh feces of a clouded leopard based on its ability to degrade cholesterol. the transcriptome profiles of g. neofelifaecis nrrl b-59395 grown with cholesterol, androstenedione (ad), and pyruvic acid were compared by rna-seq. the sterol catabolic genes are highly conserved in g. neofelifaecis, rhodococcus jostii rha1, and mycobacterium tuberculosis. the rna-seq results indicated that the genes involved in the sterol side chain ... | 2014 | 25264805 |