| molecular characterization of closteroviruses infecting cordyline fruticosa l. in hawaii. | in hawaii, common green ti plants (cordyline fruticosa l.) have been shown to harbor cordyline virus 1 (cov-1) which, along with little cherry virus 1 (lchv-1), and grapevine leafroll-associated virus 7 (glrav-7), form a distinct clade within the family closteroviridae. preliminary work has indicated that, aside from cov-1, three additional closteroviruses may infect common green ti plants in hawaii. in this study, pyrosequencing was used to characterize the genomes of closteroviruses infecting ... | 2013 | 23467405 |
| differentiation and distribution of cordyline viruses 1-4 in hawaiian ti plants (cordyline fruticosa l.). | common green ti plants (cordyline fruticosa l.) in hawaii can be infected by four recently characterized closteroviruses that are tentative members of the proposed genus velarivirus. in this study, a reverse-transcription polymerase chain reaction (rt-pcr) assay developed to detect and distinguish cordyline virus 1 (cov-1), cov-2, cov-3, and cov-4 was used to determine: (i) the distribution of these viruses in hawaii; and (ii) if they are involved in the etiology of ti ringspot disease. one hund ... | 2013 | 23881274 |
| molecular characterization and taxonomy of grapevine leafroll-associated virus 7. | the complete nucleotide sequence of an albanian isolate of grapevine leafroll-associated virus 7 (glrav-7-alb) was determined. the viral genome consists of 16,404 nucleotides and has nine open reading frames (orfs) that potentially encode proteins, most of which are typical for members of the family closteroviridae. only the 25-kda (orf8) and 27-kda (orf9) proteins had no apparent similarity to other viral proteins in the sequence databases. the genome structure of glrav-7-alb closely resembles ... | 2011 | 22109709 |
| genomic and biological analysis of grapevine leafroll-associated virus 7 reveals a possible new genus within the family closteroviridae. | deep sequencing analysis of an asymptomatic grapevine revealed a virome containing five rna viruses and a viroid. of these, grapevine leafroll-associated virus 7 (glrav-7), an unassigned closterovirus, was by far the most prominently represented sequence in the analysis. graft-inoculation of the infection to another grape variety confirmed the lack of the leafroll disease symptoms, even though glrav-7 could be detected in the inoculated indicator plants. a 16,496 nucleotide-long genomic sequence ... | 2011 | 22056321 |
| an assemblage of closteroviruses infects hawaiian ti (cordyline fruticosa l.). | the ti plant (cordyline fruticosa l.) is culturally important throughout most of polynesia and has considerable economic importance in hawai'i where the foliage is commonly used in cultural ceremonies as well as food and ornamental industries. in hawai'i, ringspot symptoms were recently observed on leaves of the common green variety of ti growing in kahalu'u on the island of o'ahu, and wailuku and hana on the island of maui. high molecular weight double-stranded (ds)rnas were isolated from the l ... | 2010 | 21153877 |