| characterization of bacillus thuringiensis strain bt185 toxic to the asian cockchafer: holotrichia parallela. | a new bacillus thuringiensis strain, bt185, was isolated from hebei soil samples in china. observations after transmission electron microscopy found that the strain produced spherical parasporal inclusions similar to that of the b. thuringiensis subsp. japonensis buibui strain, which showed toxicity to both anomala corpulenta and popillia japonica. the plasmid profile seen on an agarose gel revealed that bt185 contained six large bands of 191 kb, 161 kb, 104 kb, 84 kb, 56 kb, and 37 kb. sodium d ... | 2006 | 16775781 |
| improving toxicity of bacillus thuringiensis strain contains the cry8ca gene specific to anomala corpulenta larvae. | the cry8c-type gene designated cry8ca2, which was cloned and sequenced from a bacillus thuringiensis isolate hbf-1 in china, consisted of an open reading frame of 3483 bp encoding a protein of 1160 amino-acid residues. sequence analysis showed that the cry8ca2 protoxin of 130.5 kda had 99.9% sequence homology with the previously reported cry8ca1 protein, with one mismatch between the two amino-acid sequences. when the cry8ca2 toxin was expressed in a crystal-negative strain of b. thuringiensis ( ... | 2007 | 17805927 |
| characterization of two novel cry8 genes from bacillus thuringiensis strain bt185. | two novel cry8-type genes, cry8ea1 and cry8fa1, obtained from a holotrichia parallela-specific bacillus thuringiensis strain, bt185, were characterized. findings showed that cry8ea1 and cry8fa1 encoded polypeptides of 1164 and 1174 amino acid residues, respectively. the deduced amino acid sequences of both cry8ea1 and cry8fa1 polypeptides are the most similar to that of cry8ba1. eight conserved blocks (blocks 1-8) exist in cry8ea1 and cry8fa1 polypeptides compared with known cry proteins. cry8ea ... | 2009 | 19130127 |
| an engineered bacillus thuringiensis strain with insecticidal activity against scarabaeidae (anomala corpulenta) and chrysomelidae (leptinotarsa decemlineata and colaphellus bowringi). | a genetically-engineered bacillus thuringiensis (bt) strain, 3a-hbf, with a broad insecticidal spectrum was constructed by introducing the recombinant plasmid pstk-3a containing cry3aa7 into the wild-type bt strain hbf-1 containing the cry8ca2 gene. the cry3aa7 protein produced by strain 3a-hbf was verified by sds-page and western blotting. flat rectangular crystals of cry3aa7 protein were observed besides spherical crystals (cry8ca2). the plasmid pstk-3a was stable when strain 3a-hbf was grown ... | 2009 | 19142586 |
| construction of a bacillus thuringiensis engineered strain with high toxicity and broad pesticidal spectrum against coleopteran insects. | a newly engineered strain, denominated biot185, was constructed through integrating the cry8ca2 gene into the endogenous plasmid of bt185 (contains cry8ea1) by homologous recombination. the thermosensitive plasmid vector was eliminated by the rising temperature of recombinant cultures. no antibiotic gene or other unnecessary genes were introduced to the new strain. sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blot analysis demonstrated that the cry8ca2 gene was expressed ... | 2010 | 20165944 |
| de novo sequencing, assembly and characterization of antennal transcriptome of anomala corpulenta motschulsky (coleoptera: rutelidae). | anomala corpulenta is an important insect pest and can cause enormous economic losses in agriculture, horticulture and forestry. it is widely distributed in china, and both larvae and adults can cause serious damage. it is difficult to control this pest because the larvae live underground. any new control strategy should exploit alternatives to heavily and frequently used chemical insecticides. however, little genetic research has been carried out on a. corpulenta due to the lack of genomic reso ... | 2014 | 25461610 |
| use of redundant exclusion pcr to identify a novel bacillus thuringiensis cry8 toxin gene from pooled genomic dna. | with the aim of optimizing the cloning of novel genes from a genomic pool containing many previously identified homologous genes, we designed a redundant exclusion pcr (re-pcr) technique. in re-pcr, a pair of generic amplification primers are combined with additional primers that are designed to specifically bind to redundant, unwanted genes that are a subset of those copied by the amplification primers. during re-pcr, the specific primer blocks amplification of the full-length redundant gene. u ... | 2016 | 27084017 |
| characterization of one novel cry8 gene from bacillus thuringiensis strain q52-7. | bacillus thuringiensis (bt) is the most widely used insecticidal microbe due to its specific toxicity and safe use with respect to animals and the environment. in this study, we isolated bt strain q52-7 from a soil sample collected in the qian shan district, liao ning province, china. we observed that the q52-7 strain produced spherical crystals. the bt q52-7 strain had high toxicity against asian cockchafer (holotrichia parallela), exhibiting an lc50 of 3.80 × 10(9) cfu/g, but is not toxic for ... | 2014 | 25218711 |
| genomic sequencing identifies novel bacillus thuringiensis vip1/vip2 binary and cry8 toxins that have high toxicity to scarabaeoidea larvae. | the bacillus thuringiensis strain hbf-18 (cgmcc 2070), which has previously been shown to encode the cry8ga toxin gene, is active against both holotrichia oblita and holotrichia parallela. recombinant cry8ga however is only weakly toxic to these insect pests suggesting the involvement of additional toxins in the native strain. we report that through the use of illumina sequencing three additional, and novel, genes, namely vip1ad1, vip2ag1, and cry8-like, were identified in this strain. although ... | 2015 | 25081556 |
| cultivable gut bacteria of scarabs (coleoptera: scarabaeidae) inhibit bacillus thuringiensis multiplication. | the entomopathogen bacillus thuringiensis is used to control various pest species of scarab beetle but is not particularly effective. gut bacteria have diverse ecological and evolutionary effects on their hosts, but whether gut bacteria can protect scarabs from b. thuringiensis infection remains poorly understood. to investigate this, we isolated 32 cultivable gut bacteria from holotrichia oblita faldermann, holotrichia parallela motschulsky, and anomala corpulenta motschulsky, and analyzed thei ... | 2014 | 24780240 |
| cry8ca2-containing layer-by-layer microcapsules for the ph-controlled release of crystal protein. | to extend the activity of crystal proteins by protection from environmental stress, we developed a new type of microcapsule containing cry8ca2 protoxins. layer-by-layer (lbl) microcapsules containing cry8ca2 were successfully prepared for the first time by the alternate deposition of poly(acrylic acid) (pah) and cry8ca2 at ph 6 on the surface of poly(styrene sulphonate) (pss)-doped caco3 microbeads. scanning electron microscopy (sem) photos showed that microparticles were spherical in shape, app ... | 2014 | 24617704 |
| a genetically modified broad-spectrum strain of bacillus thuringiensis toxic against holotrichia parallela, anomala corpulenta and holotrichia oblita. | cry8ea1 from bacillus thuringiensis strain bt185 has insecticidal activity against holotrichia parallela. cry8ca2 from strain hbf-1 is effective against anomala corpulenta. cry8ga1 from strain hbf-18 is toxic to h. oblita. given the need to broaden the spectrum of b. thuringiensis, a broad-spectrum coleopteran active strain of b. thuringiensis, biot185, engineered to express multiple cry genes, including cry8ea1, cry8fa1 and cry8ca2, was created by homologous recombination introducing the cry8ca ... | 2014 | 24030167 |
| nematodospora anomalae sp. nov., a novel and d-xylose-fermenting yeast species in the lodderomyces clade. | three strains of a novel species of ascomycetous yeast were isolated from the beetle species anomala corpulenta (scarabaeoidea) collected from the baotianman and funiu mountains of china. these strains produced conjugated asci with a single coiled ascospore. phylogenetic analysis of the combined sequences of the d1/d2 domains of the large subunit rrna gene and internal transcribed spacer regions demonstrated that the three strains were closely related to nematodospora valgi and an undescribed ye ... | 2016 | 27412433 |
| correction: chemosensory gene families in adult antennae of anomala corpulenta motschulsky (coleoptera: scarabaeidae: rutelinae). | | 2015 | 26619223 |
| correction: de novo sequencing, assembly and characterization of antennal transcriptome of anomala corpulenta motschulsky (coleoptera: rutelidae). | | 2015 | 25928371 |
| chemosensory gene families in adult antennae of anomala corpulenta motschulsky (coleoptera: scarabaeidae: rutelinae). | the metallic green beetle, anomala corpulenta (coleoptera: scarabaeidae: rutelinae), is a destructive pest in agriculture and horticulture throughout asia, including china. olfaction plays a crucial role in the survival and reproduction of a. corpulenta. as a non-model species, a. corpulenta is poorly understood, and information regarding the molecular mechanisms underlying olfaction in a. corpulenta and other scarab species is scant. | 2015 | 25856077 |
| the elimination of dna from the cry toxin-dna complex is a necessary step in the mode of action of the cry8 toxin. | several crystal (cry) proteins are known to occur as dna-protein complexes. however, the role of the dna associated with the activated toxin in the mechanism of action of the cry toxin has long been ignored. here, we focused on the dna-activated cry toxin complex. both forms of the cry8ca2 and cry8ea1 toxins, i.e., with or without bound dna, were separately obtained. size-exclusion chromatography analysis indicated that the cry8ca2 toxin-dna complex has a tight or compact structure. the cry8ca2 ... | 2013 | 24324685 |
| [niches of dominant grub populations in different athletic areas of golf course]. | the study on the temporal niche, spatial niche and spatial distribution of dominant grub populations in different athletic areas of golf courses in subtropics showed that except the lower temporal niche breadths of individual grubs in greens iii and iv, the dominant grub populations in 4 areas of fairways and greens all had a >0.9 temporal niche breadth. the temporal niche breadth of 4 grub species in fairway highland and greens i and ii was the highest, and that in rough highland and green iv w ... | 2004 | 15573998 |
| [biological characteristics and damaging regularities of grubs endangering the growth of ophiopogen japonicus (thunb.) ker-gawl]. | to provide a scientific basis for prevention and control of the damage done to ophiopogen japonicus by grubs. | 1999 | 12242795 |