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complement activation and complement-dependent inflammation by neisseria meningitidis are independent of lipopolysaccharide.fulminant meningococcal sepsis has been termed the prototypical lipopolysaccharide (lps)-mediated gram-negative septic shock. systemic inflammation by activated complement and cytokines is important in the pathogenesis of this disease. we investigated the involvement of meningococcal lps in complement activation, complement-dependent inflammatory effects, and cytokine or chemokine production. whole blood anticoagulated with lepirudin was stimulated with wild-type neisseria meningitidis h44/76 (l ...200415155639
dissecting the effects of lipopolysaccharides from nonlipopolysaccharide molecules in experimental porcine meningococcal sepsis.to dissect the in vivo responses to lipopolysaccharide compared with nonlipopolysaccharide structures of whole meningococci.201020400898
neisseria meningitidis lipid a mutant lpss function as lps antagonists in humans by inhibiting tlr 4-dependent cytokine production.lipopolysaccharide is a major constituent of the outer membrane of gram-negative bacteria and important in the induction of pro-inflammatory responses. recently, novel lps species derived from neisseria meningitidis h44/76 by insertional inactivation of the lpxl1 and lpxl2 genes have been created with a lipid a portion consisting of five (penta-acylated lpxl1) or four (tetra-acylated lpxl2) fatty acids connected to the glucosamine backbone instead of six fatty acids in the wild-type lps. we show ...201021088052
a critical contribution of both cd28 and icos in the adjuvant activity of neisseria meningitidis h44/76 lps and lpxl1 lps.the development of novel vaccines against neisseria meningitidis recently gained momentum by the generation of penta-acylated lpxl1 lps which has similar adjuvant activity, but reduced endotoxic activity as compared to hexa-acylated wild type (h44/76) lps. we investigated the costimulation requirements for the adjuvant activity of both forms of lps by immunizing cd28-, icos- and b7.1/2/icos-deficient mice. both icos and cd28 appeared essential for optimal adjuvant activity of h44/76 lps or lpxl1 ...200717499399
high-level endothelial e-selectin (cd62e) cell adhesion molecule expression by a lipopolysaccharide-deficient strain of neisseria meningitidis despite poor activation of nf-kappab transcription factor.binding of host inflammatory cells to the endothelium is a critical contributor to the vascular damage characteristic of severe meningococcal disease and is regulated by endothelial cell adhesion molecules such as icam-1, vcam-1 and cd62e. intact meningococci induce far higher levels of cd62e than lipopolysaccharide (lps) alone, whereas lps is at least as potent as meningococci at inducing both vcam-1 and icam-1 expression. this suggests that meningococci possess additional factors other than lp ...200414678268
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