| organization of the regions flanking the rrna cistrons of mycoplasma strain pg50. | four clones harboring fragments of the rrna cistrons have been isolated from a genomic library of mycoplasma pg50 dna. by heteroduplex and displacement loop analysis, supplemented with sequence data, we demonstrate that the four clones together cover the two rrna cistrons of mycoplasma pg50 as well as the flanking regions. parts of the cloned fragments have been analyzed in detail, and the following conclusions have been reached. genes for trnalys and trnaleu are located about 450 bp upstream of ... | 1987 | 2444557 |
| heterogeneity of mycoplasma hominis as detected by a probe for atp genes. | use of a plasmid containing part of the atp operon of mycoplasma pg50 as a probe in southern blots show that this region can be used to detect the presence of mycoplasma species in general. dna from 14 different strains of m. hominis was analyzed for restriction fragment length polymorphism (rflp) by the use of the same pg50 probe. the m. hominis strains exhibit considerable polymorphism, although some groupings can be observed. these groupings are compared to those obtained for the same strains ... | 1987 | 2889697 |
| analysis of the mycoplasma genome by recombinant dna technology. | a library of dna fragments from mycoplasma sp. strain pg50 has been made in the vector pbr325. analysis in escherichia coli minicells of randomly picked clones from this library demonstrated that many plasmids can promote synthesis of mycoplasma protein in the e. coli genetic background. screening with labeled rrna identified plasmids with sequences from the beginning of the rrna cistron to about two-thirds within the gene for 23s rrna. use of these plasmids as probes allowed an accurate determi ... | 1984 | 6392184 |
| characterization of the 16s rrna genes from mycoplasma sp. strain f38 and development of an identification system based on pcr. | mycoplasma sp. (strain f38) is the causative agent of contagious caprine pleuropneumonia, which is a goat disease of great global concern. strain f38 belongs to the so-called "mycoplasma mycoides cluster," and the members of this cluster have many biochemical and serological properties in common, which makes it difficult to differentiate between them by conventional methods. their phylogenetic interrelationship are thus uncertain. the 16s rrna gene of the rrnb operon from strain f38 was cloned a ... | 1994 | 8169205 |
| phylogeny of the mycoplasma mycoides cluster as determined by sequence analysis of the 16s rrna genes from the two rrna operons. | the so-called mycoplasma mycoides cluster consists of six species or subspecies of mycoplasmas (mollicutes). these species are pathogenic for ruminants and some of them are of great concern in veterinary medicine. the members of the m. mycoides cluster have two rrna operons (rrna and rrnb). the nucleotide sequences of the 16s rrna genes of 10 strains, representing all of the known species and subspecies of the m. mycoides cluster, were determined by direct automated solid-phase dna sequencing. t ... | 1996 | 8763941 |