| cryptosporidium molnari n. sp. (apicomplexa: cryptosporidiidae) infecting two marine fish species, sparus aurata l. and dicentrarchus labrax l. | cryptosporidium molnari n. sp. is described from two teleost fish, the gilthead sea bream (sparus aurata l.) and the european sea bass (dicentrarchus labrax l.). the parasite was found mainly in the stomach epithelium and seldom in the intestine. oocysts were almost spherical, with four naked sporozoites and a prominent residuum, and measured 3.23-5.45 x 3.02-5.04 (mean 4.72 x 4.47) microm in the type host, gilthead sea bream (shape index 1-1.17, mean 1.05). sporulation was endogenous, as fully ... | 2002 | 12076630 |
| epidemiology of cryptosporidium molnari in spanish gilthead sea bream (sparus aurata l.) and european sea bass (dicentrarchus labrax l.) cultures: from hatchery to market size. | a long-term epidemiological study of cryptosporidium molnari in aquacultured european sea bass (esb) and gilthead sea bream (gsb) was performed in different types of facilities on the atlantic, cantabric, and mediterranean coasts. four types of studies were carried out. in study a, fish raised from juveniles to marketable size (ongrowing stage) were periodically sampled in three different types of cultures. studies b and c focused on hatchery and nursery facilities. in study d, occasional sampli ... | 2005 | 15640180 |
| interactions between bacteria and cryptosporidium molnari in gilthead sea bream (sparus aurata) under farm and laboratory conditions. | the possible interaction of cryptosporidium molnari and bacteria in gilthead sea bream (sparus aurata) was studied. epidemiological data from a pathological survey under farm conditions were analyzed. in addition, parasite and bacteria burdens were studied in experimental models in which naturally and experimentally parasitized fish were challenged with a particular strain of vibrio harveyi (h57). all the bacteria species present were studied. under farm conditions, the parasite was more prevale ... | 2006 | 16934406 |
| possible involvement of artemia as live diet in the transmission of cryptosporidiosis in cultured fish. | the viability of cryptosporidium parvum oocysts ingested by artemia franciscana metanauplii was evaluated using two fluorogenic vital dyes. there was no significant difference (p = 0.09) between the viability of oocysts maintained in saline (control) and those recovered from the digestive tract of the microcrustacean 24 h after ingestion (95 vs 90% viable oocysts). the results suggest that artemia, used as a life food in fish larviculture, may act as a vehicle for transmission of piscine cryptos ... | 2007 | 17468970 |
| molecular characterisation of a disseminated cryptosporidium infection in a koi carp (cyprinus carpio). | cryptosporidium is a protozoan parasite that infects a wide range of hosts, yet relatively little is known about the epidemiology of cryptosporidiosis in fish. here we report a disseminated cryptosporidium infection in a male koi carp (cyprinus carpio), with parasite stages identified deep within the epithelium of the intestine, kidneys, spleen, liver and gills causing severe granulomatous inflammatory lesions. molecular characterization at two loci; 18s ribosomal rna (rrna) and actin, revealed ... | 2016 | 27514884 |
| genetic diversity of cryptosporidium in fish at the 18s and actin loci and high levels of mixed infections. | cryptosporidium is an enteric parasite that infects humans and a wide range of animals. relatively little is known about the epidemiology and taxonomy of cryptosporidium in fish. in the present study, a total of 775 fish, belonging to 46 species and comprising ornamental fish, marine fish and freshwater fish were screened for the prevalence of cryptosporidium by pcr. the overall prevalence of cryptosporidium in fish was 5.3% (41/775), with prevalences ranging from 1.5 to 100% within individual h ... | 2015 | 26527238 |
| cryptosporidium huwi n. sp. (apicomplexa: eimeriidae) from the guppy (poecilia reticulata). | the morphological, biological, and molecular characteristics of cryptosporidium piscine genotype 1 from the guppy (poecilia reticulata) are described, and the species name cryptosporidium huwi n. sp. is proposed to reflect its genetic and biological differences from gastric and intestinal cryptosporidium species. oocysts of c.huwi n. sp. over-lap in size with cryptosporidium molnari, measuring approximately 4.4-4.9 µm (mean 4.6) by 4.0-4.8 µm (mean 4.4 µm) with a length to width ratio of 1.04 (0 ... | 2015 | 25637783 |
| detection of cryptosporidium molnari oocysts from fish by fluorescent-antibody staining assays for cryptosporidium spp. affecting humans. | three direct fluorescent-antibody staining assay kits for the detection of zoonotic cryptosporidium species were used to detect cryptosporidium molnari from murray cod, and the cryptosporidia were characterized by using small-subunit (ssu) ribosomal dna (rdna). to facilitate rapid diagnosis of infection, this study demonstrated that all three kits detected fresh c. molnari and two kits detected formalin-fixed oocysts. | 2011 | 21239548 |
| molecular characterization of cryptosporidium molnari reveals a distinct piscine clade. | multilocus phylogenetic analysis of small-subunit (ssu) rrna and actin from cryptosporidium molnari clustered this species with the c. molnari-like genotype of an isolate from the guppy, although the two fish isolates seem to be distinct species. the analysis of available piscine genotypes provides some support for cladistic congruence of the genus piscicryptosporidium, but additional piscine genotypes are needed. | 2010 | 20870791 |
| molecular and biological characterization of a cryptosporidium molnari-like isolate from a guppy (poecilia reticulata). | histological, morphological, genetic, and phylogenetic analyses of a cryptosporidium molnari-like isolate from a guppy (poecilia reticulata) identified stages consistent with those of c. molnari and revealed that c. molnari is genetically very distinct from all other species of cryptosporidium. this study represents the first genetic characterization of c. molnari. | 2004 | 15184187 |
| experimental transmission of cryptosporidium molnari (apicomplexa: coccidia) to gilthead sea bream (sparus aurata l.) and european sea bass (dicentrarchus labrax l.). | cryptosporidium molnari was experimentally transmitted to gilthead sea bream (sparus aurata) and european sea bass (dicentrachus labrax) by oral infection with infected stomach scrapings. the infection was also cross-transmitted from infected gilthead sea bream to sea bass by cohabitation. the course of the infection was assessed after necropsy by three microscopic diagnostic methods and their sensitivity was compared. at the end of all the experiments the prevalence of infection reached 100%. i ... | 2003 | 12923629 |