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effect of permethrin treatment of cattle on psorophora columbiae populations: preliminary field test of a host management concept.the results of a field test conducted in cooperation with the jefferson davis parish mosquito abatement district (jdpmad) during 1988 in southwestern louisiana suggested that the biweekly treatment of cattle with permethrin reduced the number of adult psorophora columbiae in nearby areas. routine mosquito control operations by jdpmad were similar from 1987 to 1988 in the cattle-treated area but increased an average of 41% in control areas. in spite of this, during the year of cattle treatment, c ...19911675250
nocturnal distribution of louisiana riceland mosquito adults.nocturnal riceland mosquito populations in southern louisiana were monitored using nonbaited cdc miniature light traps. a mixed population of anopheles quadrimaculatus, an. crucians, psorophora columbiae and culex salinarius adults was collected in a livestock occupied barn. highly variable numbers of all 4 species were trapped in areas away from the barns. species density at any given trap location was not related to trap location, type of habitat surrounding the trap or brush density near the ...19911686276
impact of minor reductions in adult and larval survival, fecundity and hatch on the population dynamics of psorophora columbiae: a simulation study.laboratory work has shown that mosquitoes obtaining blood meals from animals treated with ivermectin exhibit lowered adult survival, fecundity, egg hatch and larval survival. computer simulation evaluated the consequences of this phenomenon in field populations of psorophora columbiae feeding on cattle in the rice agroecosystem. results suggest that rather minor reductions, on the order of 10% below normal, in these life history parameters would significantly affect the population dynamics of th ...19911686448
sustained release pellets for control of culex larvae with bacillus sphaericus.bacillus sphaericus was formulated in pellets with partially hydrogenated vegetable oil, talc and a starch-based superabsorbent polymer. this formulation increased the residual activity against culex spp. larvae in large and small plots, including polluted water. when the pellets were applied to dry artificial larval habitats 5 days prior to flooding, the psorophora columbiae that hatched at the time of flooding were eliminated. after the pools were dried and reflooded, 611 bacillus colony-formi ...19911787401
efficacy of various bacillus thuringiensis var. israelensis formulations against psorophora columbiae larvae as assessed in small rice plots, 1984-88.granular, liquid and briquette formulations of bacillus thuringiensis var. israelensis were tested in small rice plots during 1984-88 against psorophora columbiae larvae. vectobac as, skeetal g, teknar hpd, abg 6172, abg 6188, abg 6193, abg 6197, abg 6199, abg 6138f and abg 6221 provided excellent control at high dosages and good control (generally greater than 85%) at relatively low concentrations. abg 6221 and abg 6172 provided excellent control at low concentrations. additional testing of the ...19901969931
effects of cattle density on new jersey light trap mosquito captures in the rice/cattle agroecosystem of southwestern louisiana.cattle are the primary host for the major pest mosquito psorophora columbiae in the rice production region of the gulf-south. annual captures of ps. columbiae, anopheles crucians and an. quadrimaculatus in new jersey light traps in acadia parish in 1984 were correlated with cattle density within 0.8 km of the trap (r2 = 97, 68 and 74%, respectively). furthermore, 7 of 10 mosquito species commonly trapped were significantly correlated with cattle density (average r2 = 82%). this work documents ho ...19901973448
efficacy of bacillus sphaericus formulations against psorophora columbiae larvae in small rice plots.bacillus sphaericus formulations were evaluated against psorophora columbiae larvae in small rice plots. all formulations tested provided good control (greater than 84%) for all rates tested at 2 days posttreatment. at 10 days posttreatment, fair control (greater than 70%) was recorded for abg-6232 (0.184 kg/ha), abg-6262; 27-242-ba (0.766 and 2.298 liters/ha), and abg-6262; 29-293-ba (2.298 liters/ha). at 7 days posttreatment mortality was below 66%. control did not exceed 27% at 15 days posttr ...19901983018
effect of topically treating cattle with permethrin on blood feeding of psorophora columbiae (diptera: culicidae) in a southwestern louisiana rice-pasture ecosystem.the percentage of resting mosquitoes that had fed on blood or were gravid-parous was determined in two pastures associated with the riceland agroecosystem in southwestern louisiana. in one pasture, all of the cattle were treated with permethrin every 14 d over a 22-wk period, whereas cattle in the other pasture were untreated. the percentage of blood-engorged psorophora columbiae (dyar & knab) was significantly lower in the vicinity of the treated herd. the effectiveness of the permethrin treatm ...19902280387
correlation between wing length and protein content of mosquitoes.wing length and protein content were compared in individual specimens of male and nonbloodfed, nongravid female mosquitoes. the following groups were tested: field-collected and colonized aedes aegypti, field-collected culex nigripalpus and aedes vexans, and colonized culex quinquefasciatus and psorophora columbiae. the correlation coefficient varied from 0.91 in ae. aegypti males to 0.98 in ae. vexans females, and in each group the p value was less than 0.001. this close correlation suggests th ...19892568393
efficacy of duplex and vectobac against psorophora columbiae and anopheles quadrimaculatus larvae in arkansas ricefields. 19892568399
distribution of bunyamwera serogroup viruses in north america, 1956-1984.we attempted to tabulate all bunyamwera serogroup (family bunyaviridae, genus bunyavirus) isolates from north america. by summarizing information from the laboratories of the centers for disease control, data generously shared by other laboratories, and the published literature, we were able to accumulate data regarding 1,372 bunyamwera serogroup viruses. these were: tensaw (664, including 8 from vertebrates), cache valley (396, including 6 from vertebrates), main drain (160, including 14 from v ...19862869708
dose-mortality responses of crawfish and mosquitoes to selected pesticides.a study was conducted to determine the toxicities (lc50s) of several pesticides on the commercially important red swamp crawfish, procambarus clarkii, and 3 mosquito species common in louisiana ricelands--anopheles quadrimaculatus, culex salinarius and psorophora columbiae. pesticides tested in laboratory bioassays included bacillus sphaericus, b. thuringiensis var. israelensis, bendiocarb, glyphosate, isostearyl alcohol, malathion, propoxur, resmethrin synergized with piperonyl butoxide (pbo) a ...19872904959
psorophora columbiae larval density in southwestern louisiana rice fields as a function of cattle density. 19872904971
computer simulation of management strategies for psorophora columbiae in the rice agroecosystem.various control strategies were evaluated using a previously-described and validated (focks et al. 1988a, 1988b) simulation model (pcsim) of the population dynamics of psorophora columbiae breeding in the rice agroecosystem. as expected from field experience, focally-applied ground or aerial ulv aerosols of insecticides were seen to provide local control of peak populations. similar control was predicted for larvicides when also used in and around urban and suburban areas. both of these measures ...19882906355
habitat selection strategies of mosquitoes inhabiting citrus irrigation furrows.the mosquitoes aedes vexans, psorophora columbiae and psorophora howardii have recently extended their habitat distribution into citrus irrigation furrows in coastal southeastern florida. oviposition site selection was examined by correlating species abundances with water depths due to rainfall or flood irrigation. psorophora columbiae and ps. howardii oviposited low in furrows, shared similar distributional relationships to water depths and were hatched by rainfall or irrigation. aedes vexans o ...19852906662
efficacy and field evaluation of bacillus thuringiensis (h-14) and b. sphaericus against floodwater mosquitoes in california.the microbial control agents bacillus thuringiensis (h-14) and b. sphaericus were evaluated in laboratory and field against psorophora columbiae. bacillus sphaericus strain 2362 was also tested in the field against aedes melanimon. psorophora columbiae was slightly more susceptible than culex quinquefasciatus to active strains of b. sphaericus. the lc90 for active strains ranged from 0.013 to 0.069 mg/liter. in field trials, aqueous suspensions of primary powder of b. sphaericus 2362 and 1593 yi ...19852906673
efficacy of bacillus sphaericus 2362 formulations against floodwater mosquitoes.four new formulations of bacillus sphaericus 2362 yielded excellent control of floodwater mosquitoes psorophora columbiae and aedes nigromaculis in irrigated fields in kings and riverside counties of california. a primary powder formulation (abg-6184) was the most active, producing excellent control of ps. columbiae and ae. nigromaculis at the rates of 0.05 to 0.5 lb/acre (0.055-0.56 kg/ha). a liquid formulation (bsp-2) was slightly less active, but was effective against the same species in the ...19883193114
beecomist-applied bacillus sphaericus for the control of riceland mosquitoes.an aerially applied flowable concentrate (fc) of bacillus sphaericus neide (isolate 2362) was evaluated against riceland mosquitoes using the beecomist spray head. application of the fc at 0.58 and 1.17 liter/ha in maturing rice fields resulted in a 48 hr mean posttreatment reduction of anopheles quadrimaculatus of 71 and 82%, respectively. a significant reduction in larval populations one week posttreatment was also observed. treatment rates of 0.29, 0.44, and 0.58 liter/ha in reflooded second ...19863507530
studies with equine infectious anemia virus: transmission attempts by mosquitoes and survival of virus on vector mouthparts and hypodermic needles, and in mosquito tissue culture.biological and mechanical transmission trials with psorophora columbiae (dyar and knab) and aedes sollicitans (walker) and ponies acutely infected with equine infectious anemia virus (eiav) were negative. the eiav antigen was detected by radioimmunoassay in ae sollicitans immediately after the mosquitoes had fed on an acutely ill pony, but not 14 days after feeding. psorophora columbiae mosquitoes had detectable eiav antigen as determined by radioimmunoassay 24 hours after they fed on an acutely ...19816119953
field evaluation of the microbial insecticide bacillus thuringiensis serotype h-14 against floodwater mosquitoes.four experimental formulations of the microbial insecticide bacillus thuringiensis var. israelensis (serotype h-14) were evaluated in the field against larvae of the floodwater mosquitoes aedes nigromaculis and psorophora columbiae with ground and aerial application methods. the formulations included abg-6108d (abbott laboratories, north chicago, ill.), a wettable powder containing about 400 iu/mg, sandoz 402-wdc (sadoz, inc., homestead, fla.), a flowable concentrate containing 600 iu/mg, and tw ...19826125127
the midgut bacterial flora of wild aedes triseriatus, culex pipiens, and psorophora columbiae mosquitoes.the midgut bacterial flora of wild-caught aedes triseriatus, culex pipiens, and psorophora columbiae mosquitoes was investigated. dissected midgut contents were examined using quantitative aerobic bacterial cultures. individual colonies (n = 134) were subcultured and identified to species. midgut bacterial counts changed dramatically during mosquito development. a 280-1,100-fold decrease in the bacterial population occurred between the larval stage and pupal emergence, whereas a subsequent 70-16 ...19968619452
isolations of potosi virus from mosquitoes collected in the united states, 1989-94.potosi (pot) virus, a recently characterized bunyamwera serogroup virus, was discovered when it was isolated from aedes albopictus collected at a waste-tire site in potosi, washington county, missouri, during 1989. during the following year, pot virus was not isolated from 39,048 mosquitoes, including 17,519 ae. albopictus, collected in washington county. in 1991, mosquito collections from south carolina, ohio, and michigan yielded 8 strains of pot virus: 6 from coquillettidia perturbans and one ...19968723251
laboratory and field plot bioassay of bacillus sphaericus against arkansas mosquito species.bacillus sphaericus was evaluated in laboratory bioassays against larvae of 7 arkansas mosquito species and in rice field plots against a single mosquito species. laboratory-tested species included aedes aegypti, ae. albopictus, ae. vexans, anopheles punctipennis, culex quinquefasciatus, cx. restuans, and psorophora columbiae. field plot bioassays were evaluated against ps. columbiae only. the laboratory formulation tested demonstrated activity against all 7 species. mortalities were observed at ...19968827596
chorionic morphology of eggs of the psorophora confinnis complex in the united states. ii. pre- and postdeposition studies of psorophora columbiae (dyar and knab) eggs.scanning and transmission electron microscopic techniques were used to determine the morphology of the exochorion and endochorion of psorophora columbiae eggs as found in the ovary and after deposition. in the ovary, the outer chorionic reticulations and tubercles of the eggs are ornately developed below the follicular epithelium. expansion of the eggs after deposition causes the outer chorionic reticuli and sculpturing (i.e., minor and major tubercles) to form the characteristic spinose appeara ...19989599323
comparative effectiveness of three adult mosquito sampling methods in habitats representative of four different biomes of california.the effectiveness of new jersey (nj) light, dry ice baited, and gravid female traps for collecting adult mosquitoes was compared at representative habitats in the coachella, san joaquin, and sacramento valleys and the los angeles basin of california. the nj light traps effectively sampled anopheles freeborni, culex tarsalis, psorophora columbiae, and several aedes when abundance was high in rural areas with minimal competitive illumination. dry ice-baited encephalitis virus surveillance or cdc s ...199910342265
ingested blood contributes to the specificity of the symbiosis of aeromonas veronii biovar sobria and hirudo medicinalis, the medicinal leech.hirudo medicinalis, the medicinal leech, usually carries in its digestive tract a pure culture of aeromonas veronii bv. sobria. such specificity is unusual for digestive tracts that are normally colonized by a complex microbial consortium. important questions for the symbiotic interaction and for the medical application after microvascular surgery are whether other bacteria can proliferate or at least persist in the digestive tract of h. medicinalis and what factors contribute to the reported sp ...200011055917
arbovirus surveillance in south carolina, 1996-98.arboviruses isolated and identified from mosquitoes in south carolina (usa) are described, including new state records for eastern equine encephalitis virus (eee), st. louis encephalitis virus (sle), flanders virus, tensaw virus (ten), and a variant of jamestown canyon virus (jc). mosquitoes were collected at 52 locations in 30 of 46 south carolina counties beginning in june 1996, and ending in october 1998, and tested for arboviruses. of 1,329 mosquito pools tested by virus isolation (85,806 mo ...200111345423
efficacy of vectolex wdg against anopheles quadrimaculatus and psorophora columbiae larvae in arkansas and mississippi rice.in 1999, an aerial application of vectolex wdg (water-dispersible granules) at 1.68 and 0.56 kg/ha, applied against sentinel 3rd-stage larvae of psorophora columbiae installed in 0.42-ha rice plots 48 h after treatment, provided no control at 72 and 96 h after treatment. less than 10% reduction was obtained at both rates 8 and 9 days after treatment against larvae of ps. columbiae installed at 7 days after treatment. in a later test, vectolex wdg manually applied at 5.04 and 1.68 kg/ha to small ...200111804459
an aquatic light trap designed for live capture of predatory tropisternus sp (coleoptera: hydrophilidae) larvae in arkansas rice fields.construction of an aquatic light trap developed for the live capture of 3rd-stage larvae of predatory tropisternus sp. for use in laboratory bioassays against larvae of anopheles quadrimaculatus and psorophora columbiae is described. on 10 occasions, an average of 5.2 traps was used per evening, resulting in 52 trap-nights that accumulated 106.7 h of trapping time, or an average of 10.6 h per trap. use of 2 heavy-duty alkaline d-sized batteries and appropriate in-circuit resistance effectively i ...200111804467
zooprophylaxis or zoopotentiation: the outcome of introducing animals on vector transmission is highly dependent on the mosquito mortality while searching.zooprophylaxis, the diversion of disease carrying insects from humans to animals, may reduce transmission of diseases such as malaria. however, as the number of animals increases, improved availability of blood meals may increase mosquito survival, thereby countering the impact of diverting feeds.200314565850
psorophora columbiae and psorophora toltecum (diptera: culicidae) colombian populations cannot be differentiated by isoenzymes.two populations of the mosquito psorophora columbiae from the central andean area of colombia and one population of ps. toltecum from the atlantic coast of colombia were analyzed for 11 isoenzyme markers. psorophora columbiae and ps. toltecum are two of the main vectors of venezuelan equine encephalitis. we found no conspicuous genetic differences between the two species. the relatively high gene flow levels among these populations indicate that these are not two different species or that there ...200314966689
west nile virus in california.west nile virus (wnv) was first isolated in california during july 2003 from a pool of culex tarsalis collected near el centro, imperial county. wnv transmission then increased and spread in imperial and coachella valleys, where it was tracked by isolation from pools of cx. tarsalis, seroconversions in sentinel chickens, and seroprevalence in free-ranging birds. wnv then dispersed to the city of riverside, riverside county, and to the whittier dam area of los angeles county, where it was detecte ...200415496236
landscape determinants and remote sensing of anopheline mosquito larval habitats in the western kenya highlands.in the past two decades the east african highlands have experienced several major malaria epidemics. currently there is a renewed interest in exploring the possibility of anopheline larval control through environmental management or larvicide as an additional means of reducing malaria transmission in africa. this study examined the landscape determinants of anopheline mosquito larval habitats and usefulness of remote sensing in identifying these habitats in western kenya highlands.200616480523
seasonal dynamics of four potential west nile vector species in north-central texas.a population survey was conducted from april through september 2002 on mosquito species occurring on the ray roberts greenbelt, a riparian corridor used for public recreation on the elm fork of the trinity river, in denton county, tx. geographic information system software was used to set up a stratified random sampling design based on habitat parameters. mosquitoes were collected using light traps, gravid traps, and resting boxes. a total of 29 species was collected during this study belonging ...200516599151
genetic structure of the genera psorophora (diptera: culicidae) in columbian and north american populations using isoenzymes and its2 sequences.two, a priori, psorophora columbiae and one, a priori, ps. toltecum populations from colombia were studied by means of eleven isoenzyme loci. the levels of genetic diversity, hardy-weinberg equilibrium, gene flow estimates, isolation by distance and genetic relationships among these three populations were studied and the results were as follows: 1. the gene diversity levels were high as well as very similar among the three populations indicating no differences between them. 2. within each popula ...200616915923
avian gis models signal human risk for west nile virus in mississippi.west nile virus (wnv) poses a significant health risk for residents of mississippi. physicians and state health officials are interested in new and efficient methods for monitoring disease spread and predicting future outbreaks. geographic information systems (gis) models have the potential to support these efforts. environmental conditions favorable for mosquito habitat were modeled using gis to derive wnv risk maps for mississippi. variables important to wnv dissemination were selected and cla ...200616945154
emergency mosquito aerial spray response to the 2004 florida hurricanes charley, frances, ivan, and jeanne: an overview of control results.in total, 43 aerial spray missions were conducted in 26 florida counties to control mosquito populations after each of the 4 hurricanes making landfall in florida in 2004. mosquitoes were trapped before and after each spray mission to determine the percentage (%) of control for the west nile virus vector culex nigripalpus (64.1%), the floodwater pest mosquito psorophora columbiae (69.7%), and for all species combined (67.7%). a discussion on these results and suggestions for future efforts are p ...200617067046
spatial and sex-specific dissection of the anopheles gambiae midgut transcriptome.the midgut of hematophagous insects, such as disease transmitting mosquitoes, carries out a variety of essential functions that mostly relate to blood feeding. the midgut of the female malaria vector mosquito anopheles gambiae is a major site of interactions between the parasite and the vector. distinct compartments and cell types of the midgut tissue carry out specific functions and vector borne pathogens interact and infect different parts of the midgut.200717261194
role of cattle treated with deltamethrine in areas with a high population of anopheles arabiensis in moshi, northern tanzania.malaria control measures were initiated from in october 2005 to august 2006 in the lower moshi irrigation schemes, tanzania. this manuscript reports on the entomological evaluation of the impact of pyrethroid-treated cattle in reducing the population of the anopheles arabiensis for selected houses in the lower moshi irrigation scheme.200717686176
urban habitat evaluation for west nile virus surveillance in mosquitoes in albuquerque, new mexico.as part of an ongoing mosquito surveillance program, 27 sites in the greater metropolitan albuquerque area (bernalillo county, new mexico) were trapped from may through september 2004. each site was sampled for 1 night weekly, using a standard co2-baited centers for disease control and prevention light trap and a gravid trap. captured mosquitoes were catalogued by location, species, and date, and selected pools were tested for west nile virus (wnv) by reverse transcription-polymerase chain react ...200717847847
mosquitoes associated with us department of agriculture managed wetlands on maryland's delmarva peninsula.mosquito faunal studies were carried out 2004-2005 in maryland's eastern shore wetlands managed by the us department of agriculture. the wetlands included flooded fields, inundated woodlands, and tidal salt marshes. sampling methods included larval collections, adult landing counts, and cdc light and oviposition traps. a total of 1,186 larvae and 1,363 adults identified represented 14 species. temporary pools were found to have the highest potential for rapid mosquito colonization with psorophor ...200717939519
vector competence of eastern and western forms of psorophora columbiae (diptera: culicidae) mosquitoes for enzootic and epizootic venezuelan equine encephalitis virus.venezuelan equine encephalitis virus (veev) continues to circulate enzootically in mexico with the potential to re-emerge and cause disease in equines and humans in north america. we infected two geographically distinct mosquito populations of eastern psorophora columbiae form columbiae (chiapas, mexico and texas, united states) and one mosquito population of western psorophora columbiae form toltecum (california, united states) with epizootic and enzootic ie veev and epizootic iab veev. we dete ...200818337337
cloning and molecular characterization of two invertebrate-type lysozymes from anopheles gambiae.we sequenced and characterized two novel invertebrate-type lysozymes from the mosquito anopheles gambiae. alignment and phylogenetic analysis of these and a number of related insect proteins identified through bioinformatics strategies showed a high degree of conservation of this protein family throughout the class insecta. expression profiles were examined for the two mosquito genes through semiquantitative and real-time pcr analysis. lys i-1 transcripts were found in adult females in the fat b ...200818397277
arbovirus transmission by culex nigripalpus in florida, 2005.understanding the transmission patterns of west nile and st. louis encephalitis viruses (family flaviviridae, genus flavivirus, wnv and slev) could result in an increased ability to predict transmission risk to humans. to examine transmission patterns between vector and host, we trapped mosquitoes in three florida counties from june to november 2005 by using chicken-baited lard can mosquito traps. these traps were used to monitor for presence of wnv and slev in mosquitoes and subsequent transmis ...200818533444
preference of female mosquitoes for natural and artificial resting sites.at a wetland study site in tuskegee national forest, al, resting female mosquitoes were collected from natural and artificial resting sites to identify species-specific resting sites and to evaluate various artificial resting sites for their utility in collecting resting mosquitoes. natural resting sites included small tree cavities, large tree cavities, and understory vegetation. artificial resting sites included resting boxes, fiber pots, and plastic trash cans. we collected 12,888 female mosq ...200818666530
venezuelan equine encephalitis virus in iquitos, peru: urban transmission of a sylvatic strain.enzootic strains of venezuelan equine encephalitis virus (veev) have been isolated from febrile patients in the peruvian amazon basin at low but consistent levels since the early 1990s. through a clinic-based febrile surveillance program, we detected an outbreak of veev infections in iquitos, peru, in the first half of 2006. the majority of these patients resided within urban areas of iquitos, with no report of recent travel outside the city. to characterize the risk factors for veev infection w ...200819079600
agent-based modelling of mosquito foraging behaviour for malaria control.traditional environmental management programmes require extensive coverage of larval habitats to reduce drastically the emergence of adult mosquitoes. recent studies have highlighted the impact of reduced availability of aquatic habitats on mosquito foraging for hosts and oviposition sites. in this study, we developed an agent-based model to track the status and movement of mosquitoes individually. mosquito foraging was represented as a two-stage process: random flight when the resource was not ...200919200566
implication of the mosquito midgut microbiota in the defense against malaria parasites.malaria-transmitting mosquitoes are continuously exposed to microbes, including their midgut microbiota. this naturally acquired microbial flora can modulate the mosquito's vectorial capacity by inhibiting the development of plasmodium and other human pathogens through an unknown mechanism. we have undertaken a comprehensive functional genomic approach to elucidate the molecular interplay between the bacterial co-infection and the development of the human malaria parasite plasmodium falciparum i ...200919424427
spatio-temporal cluster analysis of county-based human west nile virus incidence in the continental united states.west nile virus (wnv) is a vector-borne illness that can severely affect human health. after introduction on the east coast in 1999, the virus quickly spread and became established across the continental united states. however, there have been significant variations in levels of human wnv incidence spatially and temporally. in order to quantify these variations, we used kulldorff's spatial scan statistic and anselin's local moran's i statistic to uncover spatial clustering of human wnv incidence ...200919594928
characterization of expression, activity and role in antibacterial immunity of anopheles gambiae lysozyme c-1.there are eight lysozyme genes in the anopheles gambiae genome. transcripts of one of these genes, lysc-1, increased in anopheles gambiae cell line 4a3b by 24 h after exposure to heat-killed micrococcus luteus. lysozyme activity was also identified in conditioned media from the cell line from which the protein was purified to homogeneity using ion exchange and gel filtration. mass spectrometric analysis of the purified protein showed 100% identity to lysozyme c-1. purified lysozyme c-1 was teste ...201019932188
potential vectors of west nile virus in a semiarid environment: doña ana county, new mexico.the potential vectors of west nile virus (family flaviviridae, genus flavivirus, wnv) in doña ana county, nm, were determined during 2004 and 2005. trapping was conducted using centers for disease control and prevention miniature light-traps baited with dry ice, and gravid traps baited with a hay infusion. in addition, sentinel chickens were housed at four of the trapping locations to monitor wnv epizootic transmission. in total, 5,576 pools consisting of 115,797 female mosquitoes were tested fo ...200919960700
evaluation of a new formulation of adulticide, duet, against west nile virus vector mosquitoes.duet was evaluated against field-collected mosquito populations in st. john's county, fl, under laboratory and field conditions. ten serial dilutions ranging from 22 to 2.2e-08 microg/ml of the product were tested against aedes albopictus, ae. taeniorhynchus, culex quinquefasciatus, and psorophora columbiae. at 0.0022 microg/ml, 100% mortality was recorded for all species except for cx. quinquefasciatus. the 50% lethal concentration value for cx. quinquefasciatus was 0.0029 microg/ml (ci = 0.000 ...201020649133
detection of west nile virus rna in mosquitoes and identification of mosquito blood meals collected at alligator farms in louisiana.since 2001, alligator farms in the united states have sustained substantial economic losses because of west nile virus (wnv) outbreaks in american alligators (alligator mississippiensis). once an initial infection is introduced into captive alligators, wnv can spread among animals by contaminative transmission. some outbreaks have been linked to feeding on infected meat or the introduction of infected hatchlings, but the initial source of wnv infection has been uncertain in other outbreaks. we c ...201020695278
genetic structure of anopheles (nyssorhynchus) marajoara (diptera: culicidae) in colombia.five anopheles marajoara galvão and damasceno populations, representing diverse ecological conditions, were sampled throughout colombia and analyzed using nine hypervariable dna microsatellite loci. the overall genetic diversity (h = 0.58) was lower than that determined for some brazilian populations using the same markers. the caquetá population (colombia) had the lowest gene diversity (h = 0.48), and it was the only population at hardy-weinberg equilibrium. hardy-weinberg disequilibrium in the ...201020810825
candidate vectors and rodent hosts of venezuelan equine encephalitis virus, chiapas, 2006-2007.enzootic venezuelan equine encephalitis virus (veev) has been known to occur in mexico since the 1960s. the first natural equine epizootic was recognized in chiapas in 1993 and since then, numerous studies have characterized the etiologic strains, including reverse genetic studies that incriminated a specific mutation that enhanced infection of epizootic mosquito vectors. the aim of this study was to determine the mosquito and rodent species involved in enzootic maintenance of subtype ie veev in ...201122144461
Longevity and efficacy of bifenthrin treatment on desert-pattern US military camouflage netting against mosquitoes in a hot-arid environment.The current Department of Defense pest management system does not provide adequate protection from arthropod disease vectors to personnel deployed in support of US military operations. We hypothesized that military camouflage netting, ubiquitous around living and working areas in current US military operations in Africa and the Middle East, treated with a residual pesticide such as bifenthrin may reduce the presence of biting insects and improve the military pest management system. In this study ...201122017092
Identification of the Midgut Microbiota of An. stephensi and An. maculipennis for Their Application as a Paratransgenic Tool against Malaria.The midgut microbiota associated with Anopheles stephensi and Anopheles maculipennis (Diptera: Culicidae) was investigated for development of a paratransgenesis-based approach to control malaria transmission in Eastern Mediterranean Region (EMR). Here, we present the results of a polymerase chain reaction (PCR) and biochemical-based approaches to identify the female adult and larvae mosquitoe microbiota of these two major malaria vectors, originated from South Eastern and North of Iran. Plating ...201122163022
the sequential sampling of psorophora columbiae larvae in rice fields.sequential sampling plans were developed for larval populations of psorophora columbiae developing in rice fields during the growth of rice for a 2nd crop in southwestern louisiana. two types of plans were developed: 1) sequential counting plans to estimate the true mean of a population with a prespecified coefficient of variation and 2) sequential decision plans to assist in deciding whether the mean larval count/dip lies outside preselected upper or lower levels. the procedure by which the pla ...20082904961
laboratory and field evaluation of the igr fenoxycarb against mosquitoes.a new juvenile hormone mimic [fenoxycarb or ro13-5223 ethyl-(p-phenoxyphenoxy) ethylcarbamate] was evaluated in the field against culex tarsalis in stagnant water ponds, psorophora columbiae and aedes melanimon in irrigated pastures. this material, showing high level of activity against cx. quinquefasciatus in laboratory, was found to be highly effective against field populations of the 3 former species. a 5% attaclay granular formation yielded 100% inhibition of emergence in cx. tarsalis at the ...20143880261
effects of sprinkle irrigation on the occurrence and abundance of psorophora columbiae eggs in ricelands.low-pressure sprinkler irrigation was investigated as to its potential influence on the frequency of occurrence and relative abundance of psorophora columbiae eggs in texas ricelands. analysis of egg sampling data collected from soybean, rice and sorghum field sites subjected to either sprinkle or flood irrigation practices during 1982-83 indicated no significant differences in either the frequency of occurrence or relative abundance of ps. columbiae eggs in the various sites. data collected dur ...20102906684
diversity and function of bacterial microbiota in the mosquito holobiont.mosquitoes (diptera: culicidae) have been shown to host diverse bacterial communities that vary depending on the sex of the mosquito, the developmental stage, and ecological factors. some studies have suggested a potential role of microbiota in the nutritional, developmental and reproductive biology of mosquitoes. here, we present a review of the diversity and functions of mosquito-associated bacteria across multiple variation factors, emphasizing recent findings. mosquito microbiota is consider ...201323688194
culex pipiens development is greatly influenced by native bacteria and exogenous yeast.culex pipiens is the most cosmopolitan mosquito of the pipiens assemblage. by studying the nature of interactions between this species and microorganisms common to its breeding environment we can unravel important pitfalls encountered during development. we tested the survival rate of larval stages, pupae and adults of a cx. pipiens colony exposed to a variety of microorganisms in laboratory conditions and assessed the transmission to offspring (f1) by those organisms that secured development up ...201627055276
vector-host interactions of culiseta melanura in a focus of eastern equine encephalitis virus activity in southeastern virginia.eastern equine encephalitis virus (eeev) causes a highly pathogenic mosquito-borne zoonosis that is responsible for sporadic outbreaks of severe illness in humans and equines in the eastern usa. culiseta (cs.) melanura is the primary vector of eeev in most geographic regions but its feeding patterns on specific avian and mammalian hosts are largely unknown in the mid-atlantic region. the objectives of our study were to: 1) identify avian hosts of cs. melanura and evaluate their potential role in ...201526327226
emergency mosquito control on a selected area in eastern north carolina after hurricane irene.natural disasters such as hurricanes may contribute to mosquito abundance and, consequently, arbovirus transmission risk. in 2011, flooding from hurricane irene in eastern north carolina (nc) resulted in increased mosquito populations that hindered recovery efforts. budget shortfalls in nc have reduced the functionality of long-term mosquito surveillance and control programs; hence, many counties rely on the federal emergency management agency for post-disaster mosquito control. this pilot study ...201425574141
predicting the mosquito species and vertebrate species involved in the theoretical transmission of rift valley fever virus in the united states.rift valley fever virus (rvfv) is a mosquito-borne virus in the family bunyaviridiae that has spread throughout continental africa to madagascar and the arabian peninsula. the establishment of rvfv in north america would have serious consequences for human and animal health in addition to a significant economic impact on the livestock industry. published and unpublished data on rvfv vector competence, vertebrate host competence, and mosquito feeding patterns from the united states were combined ...201425211133
spinosad: a biorational mosquito larvicide for use in car tires in southern mexico.car tires are important habitats for mosquito development because of the high density populations they can harbor and their presence in urban settings. water in experimental tires was treated with one of three insecticides or an untreated control. aquatic invertebrates were sampled at weekly intervals. eggs, larval and pupal samples were laboratory-reared to estimate seasonal fluctuations in aedes aegypti and ae. albopictus abundance.201222608138
modifiable risk factors for west nile virus infection during an outbreak--arizona, 2010.west nile virus (wnv) is the leading cause of mosquito-borne disease in the united states; however, risk factors for infection are poorly defined. we performed a case-control study to identify modifiable risk factors for wnv infection. case-patients (n = 49) had laboratory evidence of recent wnv infection, whereas control-subjects (n = 74) had negative wnv serology. we interviewed participants, surveyed households, and assessed environmental data. wnv infection was associated with living in or n ...022556093
entomologic investigations during an outbreak of west nile virus disease in maricopa county, arizona, 2010.entomologic investigations were conducted during an intense outbreak of west nile virus (wnv) disease in maricopa county, arizona during july 31-august 9, 2010. the investigations compared the east valley outbreak area, and a demographically similar control area in northwestern metropolitan phoenix where no human cases were reported. five mosquito species were identified in each area, and species composition was similar in both areas. significantly more culex quinquefasciatus females were collec ...201223109372
avian hosts of west nile virus in arizona.west nile virus (wnv) causes sporadic outbreaks of human encephalitis in phoenix, arizona. to identify amplifying hosts of wnv in the phoenix area, we blood-sampled resident birds and measured antibody prevalence following an outbreak in the east valley of metropolitan phoenix during summer, 2010. house sparrow (passer domesticus), house finch (haemorhous mexicanus), great-tailed grackle (quiscalus mexicanus), and mourning dove (zenaida macroura) accounted for most wnv infections among locally r ...201323857022
modeling and biological control of mosquitoes.models can be useful at many different levels when considering complex issues such as biological control of mosquitoes. at an early stage, exploratory models are valuable in exploring the characteristics of an ideal biological control agent and for guidance in data collection. when more data are available, models can be used to explore alternative control strategies and the likelihood of success. there are few modeling studies that explicitly consider biological control in mosquitoes; however, t ...017853610
detection of arboviruses and other micro-organisms in experimentally infected mosquitoes using massively parallel sequencing.human disease incidence attributed to arbovirus infection is increasing throughout the world, with effective control interventions limited by issues of sustainability, insecticide resistance and the lack of effective vaccines. several promising control strategies are currently under development, such as the release of mosquitoes trans-infected with virus-blocking wolbachia bacteria. implementation of any control program is dependent on effective virus surveillance and a thorough understanding of ...201323460918
mosquito oviposition behavior and vector control.the burden of gene transfer from one mosquito generation to the next falls on the female and her eggs. the selection of an oviposition site that guarantees egg and larval survival is a critical step in the reproductive process. the dangers associated with ephemeral aquatic habitats, lengthy droughts, freezing winters, and the absence of larval nutrition makes careful oviposition site selection by a female mosquito extremely important. mosquito species exhibit a remarkable diversity of ovipositio ...201627869724
control of aedes albopictus with attractive toxic sugar baits (atsb) and potential impact on non-target organisms in st. augustine, florida.the purpose of this study was to test the efficacy of bait stations and foliar applications containing attractive toxic sugar baits (atsb) and eugenol to control aedes albopictus. at the same time, the potential impact of these control methods was evaluated on non-target organisms. the study was conducted at five tire sites in st. augustine, florida. a. albopictus populations were significantly reduced with atsb-eugenol applications applied directly to non-flowering vegetation and as bait statio ...024122115
draft genome sequences of enterobacter sp. isolate ag1 from the midgut of the malaria mosquito anopheles gambiae.an isolate of enterobacter sp. was obtained from the microbial community within the gut of the anopheles gambiae mosquito, a major malaria vector in africa. this genome was sequenced and annotated. the genome sequences will facilitate subsequent efforts to characterize the mosquito gut microbiome.022965099
effects of co-habitation between anopheles gambiae s.s. and culex quinquefasciatus aquatic stages on life history traits.the effective measures for the control of malaria and filariasis vectors can be achieved by targeting immature stages of anopheline and culicine mosquitoes in productive habitat. to design this strategy, the mechanisms (like biotic interactions with conspecifc and heterospecific larvae) regulating mosquito aquatic stages survivorship, development time and the size of emerging adults should be understood. this study explored the effect of co-habitation between an. gambiae s.s. and cx. quinquefasc ...201222321562
trapping of rift valley fever (rvf) vectors using light emitting diode (led) cdc traps in two arboviral disease hot spots in kenya.mosquitoes' response to artificial lights including color has been exploited in trap designs for improved sampling of mosquito vectors. earlier studies suggest that mosquitoes are attracted to specific wavelengths of light and thus the need to refine techniques to increase mosquito captures following the development of super-bright light-emitting diodes (leds) which emit narrow wavelengths of light or very specific colors. therefore, we investigated if leds can be effective substitutes for incan ...201222608087
the temporal spectrum of adult mosquito population fluctuations: conceptual and modeling implications.an improved understanding of mosquito population dynamics under natural environmental forcing requires adequate field observations spanning the full range of temporal scales over which mosquito abundance fluctuates in natural conditions. here we analyze a 9-year daily time series of uninterrupted observations of adult mosquito abundance for multiple mosquito species in north carolina to identify characteristic scales of temporal variability, the processes generating them, and the representativen ...201425478861
bunyavirus-vector interactions.the bunyaviridae family is comprised of more than 350 viruses, of which many within the hantavirus, orthobunyavirus, nairovirus, tospovirus, and phlebovirus genera are significant human or agricultural pathogens. the viruses within the orthobunyavirus, nairovirus, and phlebovirus genera are transmitted by hematophagous arthropods, such as mosquitoes, midges, flies, and ticks, and their associated arthropods not only serve as vectors but also as virus reservoirs in many cases. this review present ...201425402172
biologically informed individual-based network model for rift valley fever in the us and evaluation of mitigation strategies.rift valley fever (rvf) is a zoonotic disease endemic in sub-saharan africa with periodic outbreaks in human and animal populations. mosquitoes are the primary disease vectors; however, rift valley fever virus (rvfv) can also spread by direct contact with infected tissues. the transmission cycle is complex, involving humans, livestock, and multiple species of mosquitoes. the epidemiology of rvfv in endemic areas is strongly affected by climatic conditions and environmental variables. in this res ...201627662585
susceptibility of psorophora columbiae larvae over time to parasitism by romanomermis culicivorax.the ability of romanomermis culicivorax preparasites to penetrate and infect psorophora columbiae decreased substantially after ca. 28 hours. parasitism at temperatures typical of louisiana rice fields (i.e., 26, 29, and 32 +/- 0.5 c) showed a significant linear decrease (p < 0.01) as the percentage of older larval instars increased at the times of exposure. these data emphasize the need for a synchronous field application of preparasites to challenge the rapid development of early instars of ps ...019294147
prevalence of dirofilaria immitis (nematoda: filarioidea) in mosquitoes from northeast arkansas, the united states.a mosquito survey was conducted to identify which species of mosquitoes carry dirofilaria immitis (leidy) (nematoda: filarioidea), dog heartworm, in northeast arkansas. using polymerase chain reaction, mosquitoes were analyzed for d. immitis, dirofilaria repens railliet & henry, and acanthocheilonema dracunculoides cobbold. mosquitoes were collected from april to october 2009 using black light ultraviolet traps baited with dry ice. sixteen mosquito species were identified. d. immitis was identif ...201323926787
comparison of the vector potential of different mosquito species for the transmission of heartworm, dirofilaria immitis, in rural and urban areas in and surrounding stillwater, oklahoma, u.s.a.dirofilaria immitis leidy (spirurida: onchocercidae), or heartworm, is a mosquito-borne nematode that causes a fatal disease in carnivores. although infection is preventable through prophylactic drugs, compliance and the spectre of resistance suggest vector control is a viable alternative. there were two main objectives in this study: (a) to evaluate the relationships between landscape and social factors and the number and species of heartworm-positive mosquitoes, with a specific focus on the im ...201424898348
potential for psorophora columbiae and psorophora ciliata mosquitoes (diptera: culicidae) to transmit rift valley fever virus.rift valley fever virus (rvfv) continues to pose a threat to much of the world. unlike many arboviruses, numerous mosquito species have been associated with rvfv in nature, and many species have been demonstrated as competent vectors in the laboratory. in this study, we evaluated two field-collected psorophora species, psorophora columbiae (dyar and knab) and psorophora ciliata (f.) for their potential to transmit rvfv in north america. both species were susceptible to infection after feeding on ...201526336233
defining genetic, taxonomic, and geographic boundaries among species of the psorophora confinnis (diptera: culicidae) complex in north and south america.the psorophora confinnis complex is currently composed of three species--psorophora confinnis sensu stricto (lynch arribalzaga) in south america, psorophora columbiae (dyar and knab) in north america, and psorophora jamaicensis (theobald) in the caribbean. members of the complex are of considerable importance as vectors of arboviruses, for example, venezuelan equine encephalitis virus, and are significant biting pests throughout their range. the biological and geographic boundaries of ps. confin ...201526336224
[susceptibility of thirty-one species of mosquitoes to romanomermis yunanensis].a new species of mermithidae was found parasitizing the larvae of culex tritaeniorhynchus and culex fatigans in henan, china and then named romanomermis yunanensis. thirty-one species of mosquitoes involving six genera have been tested for susceptibility to r. yunanensis and culicinae mosquito have consistently been highly susceptible. at a 1:5 ratio of mosquito larvae to nematode juveniles, the parasitisms of aedes aegypti, ae. albopictus, culex fatigans, culex tritaeniorhynchus, anopheles sine ...19921363920
evaluation of a controlled-release silicate formulation of temephos against aedes aegypti larvae in the laboratory and psorophora columbiae larvae (diptera: culicidae) in rice field plots. 19836192242
field evaluation of a novel mos-hole trap and naphtha compared with bg sentinel trap and mosquito magnet x trap to collect adult mosquitoes.the novel mos-hole trap™ with liquid attractant naphtha™ from korea was compared with bg sentinel™ trap and mosquito magnet x™ trap for field collection of adult mosquitoes in st. johns county, northeastern florida, from may to october 2013. the novel mos-hole trap baited with naphtha (liquid attractant) collected similar numbers of mosquitoes, compared with the number of mosquitoes caught by bg sentinel traps baited with bg lure™. both mos-hole and bg sentinel traps collected a significantly gr ...201525843186
larval survey of tire-breeding mosquitoes in alabama.a state-wide survey of tire-breeding mosquitoes in alabama was conducted in 2004 and 2005. tire sites in all 67 counties in the state of alabama were sampled for mosquito larvae. a total of 13,022 mosquito larvae, representing 12 species in 7 genera, was collected. the most frequently collected species were aedes albopictus (70.4%), culex territans (8.0%), and ochlerotatus triseriatus (7.1%). the following species were also collected: cx. restuans (6.0%), cx. salinarius (2.7%), orthopodomyia sig ...200617304924
stable isotope analysis of larval mosquito diets in agricultural wetlands in the coastal plain of georgia, u.s.a.previous studies have used c and n isotope ratios to investigate the use of different food resources such as plant and animal detritus by container-breeding mosquitoes. this study is the first to report on the potential food resources assimilated by larval mosquitoes in agricultural and reference wetlands. larval mosquitoes (diptera: culcidae) were sampled, along with their potential food resources, from agricultural and reference wetland habitats throughout a seasonal hydroperiod. isosource mix ...201425424257
field evaluation of colored light-emitting diodes as attractants for woodland mosquitoes and other diptera in north central florida.the attraction of mosquitoes to transmitted light from colored super-bright light-emitting diodes (leds) (100-nm bandwidth) was evaluated by comparison of capture numbers with and without carbon dioxide-baited (200 ml/min) centers for disease control (cdc) traps. traps with either colored leds or control lights were arranged in latin square designs at 2 north central florida woodland locations and checked daily during july and august 1996. when data were analyzed by species, a significant differ ...19989673921
effect of a lunar eclipse on the flight activity of mosquitoes in the upper gulf coast of texas.adult mosquito flight activity was monitored at a coastal marshland site in chambers county, tx, before, during, and after a total eclipse of the moon on august 16, 1989. a vehicle-mounted mobile interceptor trap and a stationary, dry ice-baited cdc miniature light trap were used in this monitoring effort. aedes sollicitans, anopheles crucians, culex salinarius, and psorophora columbiae were mosquito species most abundantly represented in collections made by both traps during the 12-h study peri ...19948965069
evaluation of 1-octen-3-ol and carbon dioxide as attractants for mosquitoes associated with irrigated rice fields in arkansas.field studies were conducted to determine the responses of mosquitoes associated with irrigated riceland in arkansas to 1-octen-3-ol (octenol), carbon dioxide (co2) and combinations of both. octenol was released at 4.1 (wick in) and 41.1 (wick out) mg/h and co2 at 200, 500 and 1,000 ml/min. octenol alone did not result in a significant (p greater than 0.05) increased response for any species relative to unbaited traps. an increase in co2 release rate generally resulted in an increase in collecti ...19911680151
field evaluation of four spatial repellent devices against arkansas rice-land mosquitoes.four commercially available spatial repellent devices were tested in a rice-land habitat near stuttgart, ar, after semi-field level assessments had been made at the center for medical, agricultural, and veterinary entomology, agricultural research service, us department of agriculture in gainesville, fl. off! clip-on(metofluthrin), mosquito cognito (linalool), no-pest strip (dichlorvos), and thermacell (d-cisltrans allethrin) were selected for this study from >20 candidate products. the units ba ...201424772674
comparison of three residential mosquito traps against riceland mosquitoes in southeast arkansas.field trials were conducted at 3 locations in arkansas county, ar, to compare the effectiveness of 3 residential mosquito traps, the stinger mosquito vacuum, the mosquito magnet defender, and the mosquito deleto 2500 active system, against riceland mosquitoes, specifically anopheles quadrimaculatus and psorophora columbiae. both the stinger mosquito vacuum and the mosquito deleto captured significantly more an. quadrimaculatus and total mosquitoes than did the mosquito magnet defender. the mosqu ...200919432077
efficacy of a 1:1 and 1:5 mixture of technical permethrin and piperonyl butoxide against anopheles quadrimaculatus and psorophora columbiae.anopheles quadrimaculatus and psorophora columbiae adults were treated with a 1:1 and 1:5 mixture of technical permethrin and piperonyl butoxide. these mixtures (0.00075 lb ai/acre) at 100, 200, and 300 ft. downwind of application killed a similar (p < or = 0.05) percentage of ps. columbiae ranging from 45.1 to 68.8% and 85.7 to 100.0% after 1 and 24 h posttreatment, respectively. similar results were obtained at 1 and 24 h posttreatment against an. quadrimaculatus (0.00075 lb ai/acre) where per ...19958551299
evaluation of aerial and ground-applied adulticides against mosquito species in arkansas and louisiana.mosquitoes were subjected to ultra-low volume (ulv) aerial applications of permethrin synergized with piperonyl butoxide (biomist 30:30. biomist 31:66) and cythion and ulv ground applications of permethrin synergized with piperonyl butoxide (biomist 30:30. biomist 12:60), resmethrin (scourge), and sumithrin (solo 40-os and duets 8.8-os). permethrin compounds tested aerially against anopheles quadrimaculatus were not significantly different. biomist 30:30 tested aerially against culex quinquefasc ...19947807085
combination ground and aerial adulticide applications against mosquitoes in an arkansas riceland community.simultaneous ground and aerial adulticide applications were evaluated against riceland mosquitoes in stuttgart, ar, during july 1985. naled was aerially applied at 52.6 ml/ha over 10.4 km2 surrounding the city. ground ulv applications of a mixture of malathion, han and resmethrin/pbo (1:1:0.0625) were applied within the city at a rate of 221.8 ml/min at 24 kph. adult populations of anopheles quadrimaculatus and psorophora columbiae were reduced at 24 hr but resurgence of ps. columbiae was eviden ...19862906984
efficacy of bay sir 8514, an insect growth regulator, against psorophora columbiae and anopheles quadrimaculatus in small plot and field trials.four formulations of an insect growth regulator, bay sir 8514 (a benzoylurea compound), were tested against 2 species of riceland mosquito larvae, anopheles quadrimaculatus and psorophora columbiae. formulations of 25% wp, 0.5% g and 065 ec (65 g/liter) were tested at dosages of 14, 28, 56, and 84 g ai/ha in small plots. the 14 g ai/ha rate was ineffective for all formulations while the 28, 56 and 84 g ai/ha provided 100% mortality through 48 hr posttreatment. the 84 g ai/ha rate gave complete c ...19852906653
post-hurricane rita mosquito surveillance and the efficacy of air force aerial applications for mosquito control in east texas.post-hurricane rita mosquito surveillance was carried out in 4 east texas counties to determine mosquito abundance, species composition, and need for mosquito control. subsequently, aerial applications of naled (dibrom) for mosquito control were made by the air force aerial spray flight, while continued surveillance documented the efficacy of the applications. psorophora columbiae was the predominant species in landing counts. twenty-two mosquito species were represented in light trap collection ...200818666545
oviposition habitats of psorophora columbiae in soybean fields of a texas riceland agroecosystem.soil samples were collected over a 21/2-year period from microhabitats in fields planted to soybeans in chambers county, tx, to study the oviposition behavior of psorophora columbiae. an analysis of egg collection data gathered from the processing of 1,098 soil samples suggested that, during wet years, ps. columbiae eggs tend to be scattered throughout soybean fields. during dry years, a greater abundance of eggs occur in the low areas of these types of fields. the data also suggested that ovipo ...19872904949
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