| venezuelan equine encephalitis virus infection of spiny rats. | enzootic strains of venezuelan equine encephalitis virus (veev) circulate in forested habitats of mexico, central, and south america, and spiny rats (proechimys spp.) are believed to be the principal reservoir hosts in several foci. to better understand the host-pathogen interactions and resistance to disease characteristic of many reservoir hosts, we performed experimental infections of f1 progeny from proechimys chrysaeolus collected at a colombian enzootic veev focus using sympatric and allop ... | 2005 | 15890116 |
| ecological characterization of the aquatic habitats of mosquitoes (diptera: culicidae) in enzootic foci of venezuelan equine encephalitis virus in western venezuela. | we studied the aquatic mosquito habitats in and around enzootic foci of venezuelan equine encephalitis virus (vee) in western venezuela. specimens were sampled for 5 mo in three types of vegetation: tall lowland tropical forests, short inundated/secondary growth forests, and pastures/herbaceous vegetation around forests. ground pools, flooded pastures, swamps, ponds, and canals predominated. we used a multivariate statistical approach to quantitatively assess the relationships of mosquito specie ... | 2005 | 15962775 |
| postepizootic persistence of venezuelan equine encephalitis virus, venezuela. | five years after the apparent end of the major 1995 venezuelan equine encephalitis (vee) epizootic/epidemic, focal outbreaks of equine encephalitis occurred in carabobo and barinas states of western venezuela. virus isolates from horses in each location were nearly identical in sequence to 1995 isolates, which suggests natural persistence of subtype ic vee virus (veev) strains in a genetically stable mode. serologic evidence indicated that additional outbreaks occurred in barinas state in 2003. ... | 2005 | 16485478 |
| detection of rna from a novel west nile-like virus and high prevalence of an insect-specific flavivirus in mosquitoes in the yucatan peninsula of mexico. | as part of our ongoing surveillance efforts for west nile virus (wnv) in the yucatan peninsula of mexico, 96,687 mosquitoes collected from january through december 2007 were assayed by virus isolation in mammalian cells. three mosquito pools caused cytopathic effect. two isolates were orthobunyaviruses (cache valley virus and kairi virus) and the identity of the third infectious agent was not determined. a subset of mosquitoes was also tested by reverse transcription-polymerase chain reaction (r ... | 2009 | 19141845 |
| biodiversity and influence of climatic factors on mosquitoes (diptera: culicidae) around the peixe angical hydroelectric scheme in the state of tocantins, brazil. | the influence of climatic factors on the seasonal frequency of mosquitoes (diptera: culicidae) at the peixe angical hydroelectric scheme (tocantins, brazil) was evaluated in the present paper. mosquito surveys were conducted in the municipality of peixe and in areas surrounding the reservoir in the municipalities of paranã and são salvador do tocantins during two daytime periods (10 am-12 noon and 2 pm-4 pm) and two night-time periods (6 pm-8 pm and 6 pm-10 am) over 14 months. in total, 10,840 s ... | 2010 | 20428674 |
| mosquitoes infected with dengue viruses in brazil. | dengue epidemics have been reported in brazil since 1985. the scenery has worsened in the last decade because several serotypes are circulating and producing a hyper-endemic situation, with an increase of dhf/dss cases as well as the number of fatalities. herein, we report dengue virus surveillance in mosquitoes using a flavivirus genus-specific rt-hemi-nested-pcr assay. the mosquitoes (culicidae, n = 1700) collected in the northeast, southeast and south of brazil, between 1999 and 2005, were gr ... | 2010 | 20624314 |
| universal primers for the amplification and sequence analysis of actin-1 from diverse mosquito species. | we report the development of universal primers for the reverse-transcription polymerase chain reaction (rt-pcr) amplification and nucleotide sequence analysis of actin cdnas from taxonomically diverse mosquito species. primers specific to conserved regions of the invertebrate actin-1 gene were designed after actin cdna sequences of anopheles gambiae, bombyx mori, drosophila melanogaster, and caenorhabditis elegans. the efficacy of these primers was determined by rt-pcr with the use of total rna ... | 2010 | 20649132 |
| yellow fever virus in haemagogus leucocelaenus and aedes serratus mosquitoes, southern brazil, 2008. | yellow fever virus (yfv) was isolated from haemagogus leucocelaenus mosquitoes during an epizootic in 2001 in the rio grande do sul state in southern brazil. in october 2008, a yellow fever outbreak was reported there, with nonhuman primate deaths and human cases. this latter outbreak led to intensification of surveillance measures for early detection of yfv and support for vaccination programs. we report entomologic surveillance in 2 municipalities that recorded nonhuman primate deaths. mosquit ... | 2010 | 21122222 |
| endemic venezuelan equine encephalitis in the americas: hidden under the dengue umbrella. | venezuelan equine encephalitis (vee) is an emerging infectious disease in latin america. outbreaks have been recorded for decades in countries with enzootic circulation, and the recent implementation of surveillance systems has allowed the detection of additional human cases in countries and areas with previously unknown vee activity. clinically, vee is indistinguishable from dengue and other arboviral diseases and confirmatory diagnosis requires the use of specialized laboratory tests that are ... | 2011 | 21765860 |
| urban mosquito fauna in mérida city, méxico: immatures collected from containers and storm-water drains/catch basins. | we examined the species composition and temporal occurrence of immature mosquitoes in containers and storm-water drains/catch basins from november 2011 to june 2013 in mérida city, méxico. a wide range of urban settings were examined, including residential premises, vacant lots, parking lots, and streets or sidewalks with storm-water drains/catch basins. in total, 111,776 specimens of 15 species were recorded. the most commonly collected species were aedes (stegomyia) aegypti (l.) (n = 60,961) a ... | 0 | 25429168 |
| culicidae (diptera) selection of humans, chickens and rabbits in three different environments in the province of chaco, argentina. | studies were conducted to determine the selection of humans, chickens and rabbits by culicidae in three different environments in the province of chaco, argentina. mosquitoes were collected fortnightly using cylindrical metal traps containing animal bait (chickens and rabbits). the mosquitoes were collected between june 2001-may 2002. during the same period and with the same frequency, mosquitoes biting the human operators of the traps were collected during the first 15 min of exposure within di ... | 0 | 23903970 |
| diversity of mosquito vectors (diptera: culicidae) in caxiuanã, pará, brazil. | this paper presents a study based on ecological parameters represented by diversity and richness indices applied in a community of mosquitoes (diptera: culicidae), at the national forest of caxiuanã, melgaço municipality, state of pará, in the brazilian amazon. a total of 25,433 specimens of culicids were collected in the study, from five field collection periods, over 10 months, between 2005 and 2006. specimens were collected in four heights of the forest (ground level, 8 m, 16 m, and 30 m-cano ... | 2012 | 22997514 |
| biodiversity and temporal distribution of immature culicidae in the atlantic forest, rio de janeiro state, brazil. | to increase the knowledge of biodiversity and identify larval habitats used by immature mosquitoes in the atlantic forest, we conducted a study in areas with various stages of preservation within the guapiaçu ecological reserve in cachoeiras de macacu, rio de janeiro state. the culicidae fauna were sampled during february, april, june, august, october, and december 2012; february, march, april, may, june, august, october, and december 2013; and january and march 2014. immature mosquitoes were co ... | 2016 | 27404496 |
| a deep insight into the sialome of male and female aedes aegypti mosquitoes. | only adult female mosquitoes feed on blood, while both genders take sugar meals. accordingly, several compounds associated with blood feeding (i.e. vasodilators, anti-clotting, anti-platelets) are found only in female glands, while enzymes associated with sugar feeding or antimicrobials (such as lysozyme) are found in the glands of both sexes. we performed de novo assembly of reads from adult aedes aegypti female and male salivary gland libraries (285 and 90 million reads, respectively). by mapp ... | 2016 | 26999592 |
| characterization of a novel negevirus and a novel bunyavirus isolated from culex (culex) declarator mosquitoes in trinidad. | pools of mosquitoes were tested for insect-specific viruses using cytopathic effect (cpe) assays on aedes albopictus (c6/36) cells. illumina sequencing of rna from pool tr7094, which produced extensive cpe 2 days post-infection, yielded the complete genome sequences of a previously unknown bunyavirus, designated cumuto virus (cumv), and a second virus designated wallerfield virus (walv). walv shared highest amino acid identity (60.1 %) with dezidougou virus from côte d'ivoire, a positive-sense, ... | 2013 | 24262627 |
| mosquito-host interactions during and after an outbreak of equine viral encephalitis in eastern panama. | mosquito blood meals provide information about the feeding habits and host preference of potential arthropod-borne disease vectors. although mosquito-borne diseases are ubiquitous in the neotropics, few studies in this region have assessed patterns of mosquito-host interactions, especially during actual disease outbreaks. based on collections made during and after an outbreak of equine viral encephalitis, we identified the source of 338 blood meals from 10 species of mosquitoes from aruza abajo, ... | 2013 | 24339965 |
| feeding habits of mosquitoes (diptera: culicidae) in an area of sylvatic transmission of yellow fever in the state of são paulo, brazil. | the reintroduction of sylvatic yellow fever in the state of são paulo after about six decades was confirmed in the northwestern region in 2000, where in 2008 there also occurred an important epizootic. the purpose of this study was to investigate the feeding habits of culicids potentially involved in the sylvatic transmission of the virus in this region. | 2015 | 25810711 |
| an insight into the sialome of the frog biting fly, corethrella appendiculata. | the nematocera infraorder culicomorpha is believed to have descended from bloodfeeding ancestors over 200 million years ago, generating bloodfeeding and non-bloodfeeding flies in two superfamilies, the culicoidea-containing the mosquitoes, the frog-feeding midges, the chaoboridae, and the dixidae-and the chironomoidea-containing the black flies, the ceratopogonids, the chironomidae, and the thaumaleidae. blood feeding requires many adaptations, including development of a sophisticated salivary p ... | 2013 | 24514880 |
| dramatic expansion of the black widow toxin arsenal uncovered by multi-tissue transcriptomics and venom proteomics. | animal venoms attract enormous interest given their potential for pharmacological discovery and understanding the evolution of natural chemistries. next-generation transcriptomics and proteomics provide unparalleled, but underexploited, capabilities for venom characterization. we combined multi-tissue rna-seq with mass spectrometry and bioinformatic analyses to determine venom gland specific transcripts and venom proteins from the western black widow spider (latrodectus hesperus) and investigate ... | 2014 | 24916504 |
| a deep insight into the sialotranscriptome of the mosquito, psorophora albipes. | psorophora mosquitoes are exclusively found in the americas and have been associated with transmission of encephalitis and west nile fever viruses, among other arboviruses. mosquito salivary glands represent the final route of differentiation and transmission of many parasites. they also secrete molecules with powerful pharmacologic actions that modulate host hemostasis, inflammation, and immune response. here, we employed next generation sequencing and proteome approaches to investigate for the ... | 2013 | 24330624 |
| genetic characterization of the wyeomyia group of orthobunyaviruses and their phylogenetic relationships. | phylogenetic analyses can give new insights into the evolutionary history of viruses, especially of viruses with segmented genomes. however, sequence information for many viral families or genera is still limited and phylogenies based on single or short genome fragments can be misleading. we report the first genetic analysis of all three genome segments of wyeomyia group viruses wyeomyia, taiassui, macaua, sororoca, anhembi and cachoeira porteira (bear328208) in the genus orthobunyavirus of the ... | 2012 | 22278828 |
| sialotranscriptome sequencing and analysis of anopheles sinensis and comparison with psorophora albipes sialotranscriptome (diptera: culicidae). | most of adult female mosquitoes secrete saliva to facilitate blood sucking, digestion and nutrition, and mosquito-borne disease prevention. the knowledge of classification and characteristics of sialotranscriptome genes are still quite limited. anopheles sinensis is a major malaria vector in china and southeast asian countries. in this study, the an. sinensis sialotranscriptome was sequenced using illumina sequencing technique with a total of 10 907 unigenes to be obtained and annotated in biolo ... | 2016 | 27996203 |
| first record of psorophora albipes in quintana roo, mexico. | in mexico the psorophora genus includes 24 species divided into 3 subgenera: grabhamia, janthinosoma, and psorophora. some species occur in the nearctic region of the country (northern mexico), whereas other species occur in the neotropical region (southern mexico), and a few species occur in both regions. in quintana roo, mexico, 7 species have been previously recorded: ps. confinnis s.s., ps. champerico, ps. cyanescens, ps. ferox, ps. lutzii, ps. ciliata, and ps. lineata. in october 2013, 24 f ... | 2016 | 27802408 |