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effects of cattle density on new jersey light trap mosquito captures in the rice/cattle agroecosystem of southwestern louisiana.cattle are the primary host for the major pest mosquito psorophora columbiae in the rice production region of the gulf-south. annual captures of ps. columbiae, anopheles crucians and an. quadrimaculatus in new jersey light traps in acadia parish in 1984 were correlated with cattle density within 0.8 km of the trap (r2 = 97, 68 and 74%, respectively). furthermore, 7 of 10 mosquito species commonly trapped were significantly correlated with cattle density (average r2 = 82%). this work documents ho ...19901973448
effect of topically treating cattle with permethrin on blood feeding of psorophora columbiae (diptera: culicidae) in a southwestern louisiana rice-pasture ecosystem.the percentage of resting mosquitoes that had fed on blood or were gravid-parous was determined in two pastures associated with the riceland agroecosystem in southwestern louisiana. in one pasture, all of the cattle were treated with permethrin every 14 d over a 22-wk period, whereas cattle in the other pasture were untreated. the percentage of blood-engorged psorophora columbiae (dyar & knab) was significantly lower in the vicinity of the treated herd. the effectiveness of the permethrin treatm ...19902280387
distribution of bunyamwera serogroup viruses in north america, 1956-1984.we attempted to tabulate all bunyamwera serogroup (family bunyaviridae, genus bunyavirus) isolates from north america. by summarizing information from the laboratories of the centers for disease control, data generously shared by other laboratories, and the published literature, we were able to accumulate data regarding 1,372 bunyamwera serogroup viruses. these were: tensaw (664, including 8 from vertebrates), cache valley (396, including 6 from vertebrates), main drain (160, including 14 from v ...19862869708
efficacy of granular formulations of bacillus thuringiensis (h-14) for the control of anopheles larvae in rice fields.three granular formulations of bacillus thuringiensis (h-14) were applied to dense stands of maturing and mature rice for control of anopheles crucians and an. quadrimaculatus. aerial applications of the vectobac granule (200 itu/mg) at 5.6, 11.2 and 22.4 kg/ha to 0.4 ha plots resulted in 92, 94 and 96% reduction 48 hr after application, respectively, in populations predominantly consisting of late instars. the bactimos granule (175 itu/mg) was applied by aircraft to 0.4 ha plots and by a cyclon ...19853880212
arboviruses associated with mosquitoes from nine florida counties during 1993.mosquitoes were collected for virus isolation tests from 36 sites in bradford, lake, leon, manatee, orange, osceola, pasco, putnam, and sarasota counties, fl, from april 6 through october 11, 1993. a total of 158,129 adult specimens were collected in 726 trap nights using cdc light traps, usually baited with dry ice. at least 35 species were represented, although 60% of the collections was made up of 3 species (aedes infirmatus, 6.5%; anopheles crucians, 14.4%; and culex nigripalpus, 39.4%). fou ...19968827602
eastern equine encephalitis transmission to emus (dromaius novaehollandiae) in volusia county, florida: 1992 through 1994.from may 1992 through october 1994, sera were collected from 204 domestic emus (dromaius novaehollandiae) at a ranch in volusia county, fl, and tested for antibody evidence of arboviral infection. hemagglutination-inhibition (hi) and neutralizing (nt) antibodies to eastern equine encephalitis (eee) virus were identified in sera collected during each year. in addition, hi and nt antibodies to st. louis encephalitis virus were detected in 3 naturally infected emus. isolations of eee virus were mad ...19968887222
mosquito collections following local transmission of plasmodium falciparum malaria in westmoreland county, virginia.a 63-year-old woman from colonial beach, westmoreland county, va, was diagnosed with plasmodium falciparum malaria on july 19, 1998. the woman had no history of international travel, intravenous drug use, blood transfusion, or other risk factor for contracting the disease. she seldom left the county and generally spent her evenings indoors, leading to the conclusion that she had been bitten locally by an infected mosquito. colonial beach is host to a population of migrant agricultural laborers f ...200011081649
arbovirus surveillance in south carolina, 1996-98.arboviruses isolated and identified from mosquitoes in south carolina (usa) are described, including new state records for eastern equine encephalitis virus (eee), st. louis encephalitis virus (sle), flanders virus, tensaw virus (ten), and a variant of jamestown canyon virus (jc). mosquitoes were collected at 52 locations in 30 of 46 south carolina counties beginning in june 1996, and ending in october 1998, and tested for arboviruses. of 1,329 mosquito pools tested by virus isolation (85,806 mo ...200111345423
operational studies with valent vectolex wdg, bacillus sphaericus, in three florida mosquito control districts.vectolex wdg, bacillus sphaericus (valent biosciences corporation), was evaluated in 3 florida mosquito control districts under operational conditions. application rates were 0.5 and 1.0 lb/acre and an untreated control in each district. study sites included woodland ponds, residential areas such as catch basins, and commercial business retention ponds. pretreatment and posttreatment assessments were made with a standard dipper at designated dipping stations. the most prevalent mosquitoes includ ...200212542192
transmission of eastern equine encephalomyelitis virus in central alabama.a site near tuskegee, alabama was examined for vector activity of eastern equine encephalomyelitis (eee) virus in 2001. more than 23,000 mosquitoes representing 8 genera and 34 species were collected during a 21-week period, and five species, culiseta melanura, aedes vexans, coquillettidia perturbans, culex erraticus, and uranotaenia sapphirina, were examined for the presence of virus using a nested reverse transcriptase-polymerase chain reaction for eee virus. each species was infected at vario ...200312875303
mosquito and arbovirus activity during 1997-2002 in a wetland in northeastern mississippi.the species composition and population dynamics of adult mosquitoes in a wetland near iuka, ms, were analyzed over a 6-yr period (1997-2002) and reverse transcription-polymerase chain reaction (pcr) detection rates of arboviruses determined during five of those years. blood meals of three likely vector species were identified using a pcr-based method that allows identification of the host to species. culex erraticus (dyar & knab) composed 51.9% of the population during the 6-yr period with 295 f ...200415185956
arbovirus circulation, temporal distribution, and abundance of mosquito species in two carolina bay habitats.carolina bays, a type of geomorphic feature, may be important in the ecology of mosquito vectors in south carolina. their hydrology varies from wetland habitats with marked flooding/drying regimes to permanently flooded spring-fed lakes. moreover, they possess characteristics that contribute to the support of a particularly abundant and diverse invertebrate fauna. although it has been estimated that 2,700+ bays exist in south carolina, approximately 97% have been altered; < or = 200 bays remain ...200515815146
entomopathogenic fungi for mosquito control: a review.fungal diseases in insects are common and widespread and can decimate their populations in spectacular epizootics. virtually all insect orders are susceptible to fungal diseases, including dipterans. fungal pathogens such as lagenidium, coelomomyces and culicinomyces are known to affect mosquito populations, and have been studied extensively. there are, however, many other fungi that infect and kill mosquitoes at the larval and/or adult stage. the discovery, in 1977, of the selective mosquito-pa ...200415861235
mosquito surveillance for west nile virus in southeastern wisconsin--2002.in 2001, west nile virus (wnv) was identified among dead american crows and bluejays in five counties in southeastern wisconsin. in response to the introduction of wnv, a pilot mosquito surveillance program was initiated in these five southeastern wisconsin counties during the summer of 2002. forty sites were selected for surveillance one night each week during a 17-week period. mosquitoes were collected in carbon dioxide-baited light traps and gravid traps. during the study period 31,419 mosqui ...200315931283
morphological and molecular characterization of a cypovirus (reoviridae) from the mosquito uranotaenia sapphirina (diptera: culicidae).a novel cypovirus has been isolated from the mosquito uranotaenia sapphirina (uscpv) and shown to cause a chronic infection confined to the cytoplasm of epithelial cells of the gastric ceca and posterior stomach. the production of large numbers of virions and inclusion bodies and their arrangement into paracrystalline arrays gives the gut of infected insects a distinctive blue iridescence. the virions, which were examined by electron microscopy, are icosahedral (55 to 65 nm in diameter) with a c ...200516014906
west nile virus-infected mosquitoes, louisiana, 2002.human cases of west nile virus (wnv) disease appeared in st. tammany and tangipahoa parishes in southeastern louisiana in june 2002. cases peaked during july, then rapidly declined. we conducted mosquito collections from august 3 to august 15 at residences of patients with confirmed and suspected wnv disease to estimate species composition, relative abundance, and wnv infection rates. a total of 31,215 mosquitoes representing 25 species were collected by using primarily gravid traps and co2-bait ...200516229769
distribution of anopheles albimanus, anopheles vestitipennis, and anopheles crucians associated with land use in northern belize.anthropogenic land use changes often alter natural patterns of disease transmission. the goal of this study was to determine whether phosphorus input from sugarcane, saccharum officinarum l., cultivation in northern belize could pose a significant environmental impact on malaria transmission by changing vegetation structure and composition of wetlands and associated larval habitats. our primary focus was on the increased dominance of cattail, typha domingensis pers., a favored habitat for anophe ...200616739424
is vector body size the key to reduced malaria transmission in the irrigated region of niono, mali?malaria vectors can reach very high densities in villages near irrigated rice fields in africa, leading to the expectation that malaria should be especially prevalent there. surprisingly, this is not always the case. in niono, mali, villages from nonirrigated areas have higher malaria prevalence than those within the irrigated regions, which suffer from higher mosquito numbers. one hypothesis explaining this observation is that mosquitoes from irrigated fields with high densities are inefficient ...200617017214
west nile virus infection in mosquitoes in the mid-south usa, 2002-2005.west nile virus (family flaviviridae, genus flavivirus, wnv) was first detected in the tennessee valley and in alabama in august 2001. in summer 2002, intensive viral activity was seen, but in subsequent years, viral activity settled into an enzootic pattern. here, we report an analysis of viral activity in the mosquito fauna in the mid-south from 2002 (the first year viral activity was detected in mosquitoes) through 2005. eight mosquito species were infected with wnv during 2002. however, vira ...200717294929
a high rate of natural plasmodium infection in anopheles crucians. 194617809745
mosquitoes associated with us department of agriculture managed wetlands on maryland's delmarva peninsula.mosquito faunal studies were carried out 2004-2005 in maryland's eastern shore wetlands managed by the us department of agriculture. the wetlands included flooded fields, inundated woodlands, and tidal salt marshes. sampling methods included larval collections, adult landing counts, and cdc light and oviposition traps. a total of 1,186 larvae and 1,363 adults identified represented 14 species. temporary pools were found to have the highest potential for rapid mosquito colonization with psorophor ...200717939519
arbovirus transmission by culex nigripalpus in florida, 2005.understanding the transmission patterns of west nile and st. louis encephalitis viruses (family flaviviridae, genus flavivirus, wnv and slev) could result in an increased ability to predict transmission risk to humans. to examine transmission patterns between vector and host, we trapped mosquitoes in three florida counties from june to november 2005 by using chicken-baited lard can mosquito traps. these traps were used to monitor for presence of wnv and slev in mosquitoes and subsequent transmis ...200818533444
preference of female mosquitoes for natural and artificial resting sites.at a wetland study site in tuskegee national forest, al, resting female mosquitoes were collected from natural and artificial resting sites to identify species-specific resting sites and to evaluate various artificial resting sites for their utility in collecting resting mosquitoes. natural resting sites included small tree cavities, large tree cavities, and understory vegetation. artificial resting sites included resting boxes, fiber pots, and plastic trash cans. we collected 12,888 female mosq ...200818666530
species composition and distribution of adult anopheles (diptera: culicidae) in panama.anopheles (diptera: culicidae) species composition and distribution were studied using human landing catch data over a 35-yr period in panama. mosquitoes were collected from 77 sites during 228 field trips carried out by members of the national malaria eradication service. fourteen anopheles species were identified. the highest average human biting rates were recorded from anopheles (nyssorhynchus) albimanus (wiedemann) (9.8 bites/person/night) and anopheles (anopheles) punctimacula (dyar and kn ...200818826025
evaluation of the mosquito sentinel 360 trap in florida residential environments.the mosquito sentinel 360 (ms) trap was evaluated in back yards of 6 residences in and near gainesville, fl. carbon dioxide and blue-light-emitting diode-modified centers for disease control and prevention (cdc) traps were utilized as a human substitute host to provide a measure of the effectiveness of the ms trap in reducing mosquito presence at the back door area of the houses. at 5 of the 6 residences, a ms trap was operated with or without carbon dioxide, lurex3 + octenol, or an experimental ...200819181060
two distinct triatoma dimidiata (latreille, 1811) taxa are found in sympatry in guatemala and mexico.approximately 10 million people are infected with trypanosoma cruzi, the causative agent of chagas disease, which remains the most serious parasitic disease in the americas. most people are infected via triatomine vectors. transmission has been largely halted in south america in areas with predominantly domestic vectors. however, one of the main chagas vectors in mesoamerica, triatoma dimidiata, poses special challenges to control due to its diversity across its large geographic range (from mexi ...200919274073
tensaw virus genome sequence and its relation to other bunyaviridae.tensaw virus (tsv) belongs to the genus orthobunyavirus within the bunyaviridae family. although tsv does not cause hemorrhagic fever as some other members of its family, serological studies have shown that serum from florida residents react against tsv indicating viral infection in humans. in this study, the three rna genome segments of a tsv isolated from anopheles crucians mosquitoes collected in north central florida in 2006 and a tsv isolate obtained from the cdc, fort collins, were sequenc ...200919760176
experiments on the development of malaria parasites in three american species of anopheles.since a knowledge of the susceptibility of any species of anopheles to infection with malaria parasites is of great importance in determining its part in the transmission of malaria, the experiments reported here were undertaken, and included the three most prevalent species of this genus occurring in the united states. as a result of these experiments anopheles punctipennis is shown to be an efficient host of the organisms of tertian and estivo-autumnal malaria, anopheles crucians of estivo-aut ...191619868018
blood feeding patterns of mosquitoes: random or structured?abstract:201020205866
linking environmental nutrient enrichment and disease emergence in humans and wildlife.worldwide increases in human and wildlife diseases have challenged ecologists to understand how large-scale environmental changes affect host-parasite interactions. one of the most profound changes to earth's ecosystems is the alteration of global nutrient cycles, including those of phosphorus (p) and especially nitrogen (n). along with the obvious direct benefits of nutrient application for food production, anthropogenic inputs of n and p can indirectly affect the abundance of infectious and no ...201020349828
incrimination of the mosquito, aedes taeniorhynchus, as the primary vector of heartworm, dirofilaria immitis, in coastal yucatan, mexico.mosquito collections were carried out on microfilaraemic dogs, positive for dirofilaria sp., for 18 consecutive nights in the coastal town of celestún, yucatan, southeast mexico, during the rainy season (august) of 2007. a total of 292 female mosquitoes representing 12 species of dipteran culicidae were collected: anopheles albimanus (wiedemann); anopheles crucians (wiedemann); anopheles pseudopunctipennis (theobald); culex coronator (dyar & knab); culex interrogator (dyar & knab); culex nigripa ...201020572932
detection of west nile virus rna in mosquitoes and identification of mosquito blood meals collected at alligator farms in louisiana.since 2001, alligator farms in the united states have sustained substantial economic losses because of west nile virus (wnv) outbreaks in american alligators (alligator mississippiensis). once an initial infection is introduced into captive alligators, wnv can spread among animals by contaminative transmission. some outbreaks have been linked to feeding on infected meat or the introduction of infected hatchlings, but the initial source of wnv infection has been uncertain in other outbreaks. we c ...201020695278
detection of dirofilaria immitis (nematoda: filarioidea) by polymerase chain reaction in aedes albopictus, anopheles punctipennis, and anopheles crucians (diptera: culicidae) from georgia, usa.potential mosquito vectors of dirofilaria immitis (leidy) (nematoda: filarioidea), the causative agent of dog heartworm in the southeastern region of the united states, were collected with cdc light traps and gravid traps in seven counties in the state of georgia, usa. the presence of d. immitis in these mosquitoes was detected by polymerase chain reaction using species-specific primers for the d. immitis surface or cuticular antigen. overall, 1,574 mosquitoes of 13 species in seven genera were ...201020695279
a high rate of natural plasmodium infection in anopheles crucians. 194621065118
genome-wide profiling of diel and circadian gene expression in the malaria vector anopheles gambiae.anopheles gambiae, the primary african vector of malaria parasites, exhibits numerous rhythmic behaviors including flight activity, swarming, mating, host seeking, egg laying, and sugar feeding. however, little work has been performed to elucidate the molecular basis for these daily rhythms. to study how gene expression is regulated globally by diel and circadian mechanisms, we have undertaken a dna microarray analysis of an. gambiae under light/dark cycle (ld) and constant dark (dd) conditions. ...201121715657
field evaluation of a large-scale barrier application of bifenthrin on a golf course to control floodwater mosquitoes.in order to better understand the role a barrier application plays in an integrated mosquito management program, we applied a bifenthrin barrier application to a large golf course community and evaluated the mosquito population, service requests, and money spent for control from our barrier site as well as a site that was only treated via ground ultra-low-volume (ulv) application or that was left as a control site. both the barrier application (t = 2.63, df = 126, p = 0.0147) and the ground ulv ...201223833902
host associations of mosquitoes at eastern equine encephalitis virus foci in connecticut, usa.eastern equine encephalitis virus (eeev) is a highly pathogenic mosquito-borne arbovirus, with active transmission foci in freshwater hardwood swamps in eastern north america, where enzootic transmission is maintained between the ornithophilic mosquito, culiseta melanura, and wild passerine birds. the role of other locally abundant mosquito species in virus transmission and their associations with vertebrate hosts as sources of blood meals within these foci are largely unknown but are of importa ...201627577939
vector-host interactions of culiseta melanura in a focus of eastern equine encephalitis virus activity in southeastern virginia.eastern equine encephalitis virus (eeev) causes a highly pathogenic mosquito-borne zoonosis that is responsible for sporadic outbreaks of severe illness in humans and equines in the eastern usa. culiseta (cs.) melanura is the primary vector of eeev in most geographic regions but its feeding patterns on specific avian and mammalian hosts are largely unknown in the mid-atlantic region. the objectives of our study were to: 1) identify avian hosts of cs. melanura and evaluate their potential role in ...201526327226
ecology of culiseta melanura and other mosquitoes (diptera: culicidae) from walton county, fl, during winter period 2013-2014.winter ecology of putative vectors of eastern equine encephalomyelitis virus (eeev) in northern florida was investigated at field locations with evidence of historic eeev winter transmission. light traps and resting shelters were used to sample the mosquito community in the vicinity of eight sentinel flocks throughout the winter period (november-april) of 2013 and 2014 in walton county, fl. overall mosquito activity was relatively low, although mosquitoes were captured during each week of the st ...201526336227
field investigations of winter transmission of eastern equine encephalitis virus in florida.studies investigating winter transmission of eastern equine encephalitis virus (eeev) were conducted in hillsborough county, florida. the virus was detected in culiseta melanura and anopheles quadrimaculatus in february 2012 and 2013, respectively. during the winter months, herons were the most important avian hosts for all mosquito species encountered. in collections carried out in the summer of 2011, blood meals taken from herons were still common, but less frequently encountered than in winte ...201425070997
habitat associations of eastern equine encephalitis transmission in walton county florida.eastern equine encephalitis virus (eeev; family togaviridae, genus alphavirus) a highly pathogenic mosquito-borne virus is endemic to eastern north america. the ecology of eeev in florida differs from that in other parts of the united states; eeev in the northeastern united states is historically associated with freshwater wetlands. no formal test of habitat associations of eeev in florida has been reported. geographical information sciences (gis) was used in conjunction with sentinel chicken ee ...022679885
urban mosquito fauna in mérida city, méxico: immatures collected from containers and storm-water drains/catch basins.we examined the species composition and temporal occurrence of immature mosquitoes in containers and storm-water drains/catch basins from november 2011 to june 2013 in mérida city, méxico. a wide range of urban settings were examined, including residential premises, vacant lots, parking lots, and streets or sidewalks with storm-water drains/catch basins. in total, 111,776 specimens of 15 species were recorded. the most commonly collected species were aedes (stegomyia) aegypti (l.) (n = 60,961) a ...025429168
predicting the mosquito species and vertebrate species involved in the theoretical transmission of rift valley fever virus in the united states.rift valley fever virus (rvfv) is a mosquito-borne virus in the family bunyaviridiae that has spread throughout continental africa to madagascar and the arabian peninsula. the establishment of rvfv in north america would have serious consequences for human and animal health in addition to a significant economic impact on the livestock industry. published and unpublished data on rvfv vector competence, vertebrate host competence, and mosquito feeding patterns from the united states were combined ...201425211133
ecology of potential west nile virus vectors in southeastern louisiana: enzootic transmission in the relative absence of culex quinquefasciatus.a study of west nile virus (wnv) ecology was conducted in st. tammany parish, louisiana, from 2002 to 2004. mosquitoes were collected weekly throughout the year using centers for disease control and prevention (cdc) light traps placed at 1.5 and 6 m above the ground and gravid traps. a total of 379,466 mosquitoes was collected. wnv was identified in 32 pools of mosquitoes comprising four species; 23 positive pools were from culex nigripalpus collected during 2003. significantly more positive poo ...201323478575
anthropogenic impacts on mosquito populations in north america over the past century.the recent emergence and spread of vector-borne viruses including zika, chikungunya and dengue has raised concerns that climate change may cause mosquito vectors of these diseases to expand into more temperate regions. however, the long-term impact of other anthropogenic factors on mosquito abundance and distributions is less studied. here, we show that anthropogenic chemical use (ddt; dichlorodiphenyltrichloroethane) and increasing urbanization were the strongest drivers of changes in mosquito ...201627922001
north american wetlands and mosquito control.wetlands are valuable habitats that provide important social, economic, and ecological services such as flood control, water quality improvement, carbon sequestration, pollutant removal, and primary/secondary production export to terrestrial and aquatic food chains. there is disagreement about the need for mosquito control in wetlands and about the techniques utilized for mosquito abatement and their impacts upon wetlands ecosystems. mosquito control in wetlands is a complex issue influenced by ...201223222252
blood feeding patterns of potential arbovirus vectors of the genus culex targeting ectothermic hosts.reptiles and amphibians constitute a significant portion of vertebrate biomass in terrestrial ecosystems and may be important arbovirus reservoirs. to investigate mosquito preference for ectothermic hosts, feeding indices were calculated from data collected in tuskegee national forest, alabama, usa. four mosquito species fed upon ectothermic hosts, with culex peccator and cx. territans feeding primarily upon ectotherms. these two species appeared to target distinct species with little overlap in ...018981528
evaluation of attractive toxic sugar bait (atsb)-barrier for control of vector and nuisance mosquitoes and its effect on non-target organisms in sub-tropical environments in florida.the efficacy of attractive toxic sugar baits (atsb) with the active ingredient eugenol, an environmental protection agency exempt compound, was evaluated against vector and nuisance mosquitoes in both laboratory and field studies. in the laboratory, eugenol combined in attractive sugar bait (asb) solution provided high levels of mortality for aedes aegypti, culex quinquefasciatus, and anopheles quadrimaculatus. field studies demonstrated significant control: >70% reduction for aedes atlanticus, ...201324361724
sex-biased avian host use by arbovirus vectors.prevalence of arthropod-borne parasites often differs drastically between host sexes. this sex-related disparity may be related to physiological (primarily hormonal) differences that facilitate or suppress replication of the pathogen in host tissues. alternately, differences in pathogen prevalence between host sexes may be owing to differential exposure to infected vectors. here, we report on the use of pcr-based assays recognizing bird sex chromosomes to investigate sex-related patterns of avia ...201426064562
modeling dynamics of culex pipiens complex populations and assessing abatement strategies for west nile virus.the primary mosquito species associated with underground stormwater systems in the united states are the culex pipiens complex species. this group represents important vectors of west nile virus (wnv) throughout regions of the continental u.s. in this study, we designed a mathematical model and compared it with surveillance data for the cx. pipiens complex collected in beaufort county, south carolina. based on the best fit of the model to the data, we estimated parameters associated with the eff ...201425268229
ecophysiology of anopheles gambiae s.l.: persistence in the sahel.the dry-season biology of malaria vectors is poorly understood, especially in arid environments when no surface waters are available for several months, such as during the dry season in the sahel. here we reappraise results on the dry-season physiology of members of the anopheles gambiae s.l. complex in the broad context of dormancy in insects and especially in mosquitoes. we examine evidence on seasonal changes in reproduction, metabolism, stress tolerance, nutrition, molecular regulation, and ...201424933461
diversity of mosquitoes and the aquatic insects associated with their oviposition sites along the pacific coast of mexico.the abundance, richness and diversity of mosquitoes and aquatic insects associated with their oviposition sites were surveyed along eight states of the pacific coast of mexico. diversity was estimated using the shannon index (h'), similarity measures and cluster analysis.201424450800
analyzing mosquito (diptera: culicidae) diversity in pakistan by dna barcoding.although they are important disease vectors mosquito biodiversity in pakistan is poorly known. recent epidemics of dengue fever have revealed the need for more detailed understanding of the diversity and distributions of mosquito species in this region. dna barcoding improves the accuracy of mosquito inventories because morphological differences between many species are subtle, leading to misidentifications.201424827460
functional constraints and evolutionary dynamics of the repeats in the rdna internal transcribed spacer 2 of members of the anopheles barbirostris group.the anopheles barbirostris group is widely distributed in southeast asia. although seven species have been formally described, a molecular analysis of the rdna its2 and the mitochondrial cytochrome oxidase i gene suggests that the group includes species that are morphologically very similar or identical.we have previously shown that species in the anopheles barbirostris subgroup have an exceptionally large its2 (>1.5 kb), greater than in any other anopheline group. however, the molecular process ...201424646478
variation in number and formation of repeat sequences in the rdna its2 region of five sibling species in the anopheles barbirostris complex in thailand.repeat sequences of approximately 100 base pairs in length were found in the rdna its2 region of anopheles barbirostris van der wulp (diptera: culicidae) species a1, a2, a3, a4, and an. campestris-like in the an. barbirostris complex. variation in the number of repeats was observed among the five sibling species. specifically, 10 repeats were observed in a1, eight in a2, a4, and campestris-like, and three in a3. based on similarities in the sequences of the repeats, related repeats were classifi ...022233453
human and animal dirofilariasis: the emergence of a zoonotic mosaic.dirofilariasis represents a zoonotic mosaic, which includes two main filarial species (dirofilaria immitis and d. repens) that have adapted to canine, feline, and human hosts with distinct biological and clinical implications. at the same time, both d. immitis and d. repens are themselves hosts to symbiotic bacteria of the genus wolbachia, the study of which has resulted in a profound shift in the understanding of filarial biology, the mechanisms of the pathologies that they produce in their hos ...022763636
mosquito oviposition behavior and vector control.the burden of gene transfer from one mosquito generation to the next falls on the female and her eggs. the selection of an oviposition site that guarantees egg and larval survival is a critical step in the reproductive process. the dangers associated with ephemeral aquatic habitats, lengthy droughts, freezing winters, and the absence of larval nutrition makes careful oviposition site selection by a female mosquito extremely important. mosquito species exhibit a remarkable diversity of ovipositio ...201627869724
larval habitat characterization of anopheles darlingi from its northernmost geographical distribution in chiapas, mexico.anopheles darlingi is considered the most efficient malaria vector in the neotropical region. in mexico, its role as an incriminated vector of plasmodium has not been confirmed in the lacandon forest. similarly, knowledge about bionomic and larval ecology is scarce. the study aim was to identify and describe the larval habitats of an. darlingi in chiapas, méxico.201526693816
control of aedes albopictus with attractive toxic sugar baits (atsb) and potential impact on non-target organisms in st. augustine, florida.the purpose of this study was to test the efficacy of bait stations and foliar applications containing attractive toxic sugar baits (atsb) and eugenol to control aedes albopictus. at the same time, the potential impact of these control methods was evaluated on non-target organisms. the study was conducted at five tire sites in st. augustine, florida. a. albopictus populations were significantly reduced with atsb-eugenol applications applied directly to non-flowering vegetation and as bait statio ...024122115
the field evaluation of a push-pull system to control malaria vectors in northern belize, central america.campaigns for the continued reduction and eventual elimination of malaria may benefit from new and innovative vector control tools. one novel approach being considered uses a push-pull strategy, whereby spatial repellents are used in combination with outdoor baited traps. the desired effect is the behavioural manipulation of mosquito populations to elicit movement of vectors away from people and into traps.201525925395
malaria vector research and control in haiti: a systematic review.haiti has a set a target of eliminating malaria by 2020. however, information on malaria vector research in haiti is not well known. this paper presents results from a systematic review of the literature on malaria vector research, bionomics and control in haiti.201627443992
identification of a novel subtype of south river virus (family bunyaviridae).we previously reported the isolation of south river virus (sorv) from a pool of mosquitoes collected in the yucatan peninsula of mexico (farfan-ale et al. in vector borne zoonotic dis 10:777-783, 5). the isolate (designated sorv-252) was identified as sorv after a 197-nucleotide region of its small rna genome segment was sequenced. in the present study, the complete small and medium rna genome segments and part of the large rna genome segment of sorv-252 were sequenced and shown to have 92%, 85% ...201222411100
genetic diversity of plasmodium vivax and plasmodium falciparum in honduras.understanding the population structure of plasmodium species through genetic diversity studies can assist in the design of more effective malaria control strategies, particularly in vaccine development. central america is an area where malaria is a public health problem, but little is known about the genetic diversity of the parasite's circulating species. this study aimed to investigate the allelic frequency and molecular diversity of five surface antigens in field isolates from honduras.201223181845
malaria elimination in haiti by the year 2020: an achievable goal?haiti and the dominican republic, which share the island of hispaniola, are the last locations in the caribbean where malaria still persists. malaria is an important public health concern in haiti with 17,094 reported cases in 2014. further, on january 12, 2010, a record earthquake devastated densely populated areas in haiti including many healthcare and laboratory facilities. weakened infrastructure provided fertile reservoirs for uncontrolled transmission of infectious pathogens. this situatio ...201526043728
potential for mosquitoes (diptera: culicidae) from florida to transmit rift valley fever virus.we evaluated aedes atlanticus dyar and knab, aedes infirmatus dyar and knab, aedes vexans (meigen), anopheles crucians wiedemann, coquillettidia perturbans (walker), culex nigripalpus theobald, mansonia dyari belkin, heinemann, and page, and psorophora ferox (von humboldt) from florida to determine which of these species should be targeted for control should rift valley fever virus (rvfv) be detected in north america. female mosquitoes that had fed on adult hamsters inoculated with rvfv were inc ...201324180117
evaluation of various models of propane-powered mosquito traps.large cage and field studies were conducted to determine the efficacy of various models of propane-powered mosquito traps. these traps utilized counterflow technology in conjunction with catalytic combustion to produce attractants (carbon dioxide, water vapor, and heat) and a thermoelectric generator that converted excess heat into electricity for stand-alone operation. the cage studies showed that large numbers of aedes aegypti and ochlerotatus taeniorhynchus were captured and that each progres ...200212125861
comparative analysis of gut microbiota of mosquito communities in central illinois.the composition and structure of microbial communities that inhabit the mosquito midguts are poorly understood despite their well-documented potential to impede pathogen transmission.201728245239
implications for operational control of adult mosquito production in cisterns and wells in st. augustine, fl using attractive sugar baits.the aim of this study was to further investigate the use of attractive sugar baits as an effective, inexpensive, and environmentally friendly tool for integrated mosquito management programs. mosquitoes were offered dyed sugar bait in wells and cisterns in an urban tourist area in st. augustine, fl. exit traps were constructed to cover the well and cistern openings so the number of resting and emerging mosquitoes stained by feeding on the sugar bait could be monitored. four mosquito species were ...201222820024
efficacy of three attractant blends tested in combination with carbon dioxide against natural populations of mosquitoes and biting flies at the lower suwannee wildlife refuge.synthetic blends of chemicals identified previously from human skin emanations were evaluated against mosquito and biting fly populations at the lower suwannee wildlife refuge near cedar key, fl. mosquito magnet-experimental traps were baited with the red (400 ml acetone: 10 ml 1-hexen-3-ol:10 ml 1-octen-3-ol), blue (400 ml acetone: 1 g/liter lactic acid:20 ml glycolic acid), or green blend (400 ml acetone:1.5 g/liter lactic acid:20 ml dimethyl disulfide) plus co2 or with co2 alone (control). a ...201222894125
ineffectiveness of mass trapping for mosquito control in st. andrews state park, panama city beach, florida.abstract. mass trapping with multiple co2- and octenol-supplemented mosquito magnet x traps (mm-x), operated 24 h/day, 7 days/wk, from march through november 2008, at st. andrews state park on northwest florida's gulf coast, did not significantly reduce mosquito numbers compared to nontreated control sites. anopheles crucians, aedes taeniorhynchus, culex salinarius, and culex erraticus were the predominant species. culex coronator was also collected for the first time in the park. failure to red ...201020402350
methylmercury in mosquitoes related to atmospheric mercury deposition and contamination.a connection between loadings of inorganic hg, especially from the atmosphere, and accumulation of methylmercury (mehg) in aquatic biota has not been firmly established. mosquitoes (diptera: culicidae) may be a useful indictor of hg contamination or mehg accumulation in aquatic ecosystems because they have aquatic life stages, and their ubiquitous distribution permits sampling across wide ranges of climate, biological productivity, and atmospheric hg deposition. we examined mehg in adult mosquit ...200515926549
ribosomal dna its2 sequences differentiate six species in the anopheles crucians complex (diptera: culicidae).anopheles crucians wiedemann (sensu lato) was investigated for the presence of cryptic species using rdna its2 sequences. this complex of species presently contains the named species an. crucians, an. bradleyi king, and an. georgianus king. adult female mosquitoes were collected at 28 sites in alabama, florida, georgia, north carolina, mississippi, and louisiana, resulting in 245 progeny broods. species were identified using preliminary morphological characters, and the internal transcribed spac ...200415185940
comparison of two american biophysics mosquito traps: the professional and a new counterflow geometry trap.large cage and field studies were conducted to compare the efficacy of 2 american biophysics corporation mosquito traps, the standard professional (pro) trap and a new counterflow geometry (cfg) trap. the pro trap utilizes conventional downdraft technology and the cfg trap uses a patent-pending technology. in large cage studies, similarly baited cfg traps captured approximately 1.7 times as many laboratory-reared aedes taeniorhynchus as the pro trap. the cfg trap baited with co2 + octenol result ...199910480115
field evaluation of colored light-emitting diodes as attractants for woodland mosquitoes and other diptera in north central florida.the attraction of mosquitoes to transmitted light from colored super-bright light-emitting diodes (leds) (100-nm bandwidth) was evaluated by comparison of capture numbers with and without carbon dioxide-baited (200 ml/min) centers for disease control (cdc) traps. traps with either colored leds or control lights were arranged in latin square designs at 2 north central florida woodland locations and checked daily during july and august 1996. when data were analyzed by species, a significant differ ...19989673921
effect of a lunar eclipse on the flight activity of mosquitoes in the upper gulf coast of texas.adult mosquito flight activity was monitored at a coastal marshland site in chambers county, tx, before, during, and after a total eclipse of the moon on august 16, 1989. a vehicle-mounted mobile interceptor trap and a stationary, dry ice-baited cdc miniature light trap were used in this monitoring effort. aedes sollicitans, anopheles crucians, culex salinarius, and psorophora columbiae were mosquito species most abundantly represented in collections made by both traps during the 12-h study peri ...19948965069
seasonal abundance, age composition, and body size of salt-marsh anopheles (diptera: culicidae) in south florida.five co2-baited light traps operated fortnightly between november 1989 and may 1991 near a salt marsh in vero beach, fl, caught 1,961 anopheles crucians wiedemann (s.l.) and 691 an. atropos dyar & knab. both species were most abundant in winter and least abundant in summer; their numbers correlated inversely with lagged mean ambient temperatures. annual parous rates were 55% for an. crucians and 58% for an. atropos and varied little seasonally. wing lengths of female an. crucians measured for ei ...19938254635
photoperiod and longevity in anopheles crucians.the effect of photoperiod on longevity of nondiapausing members of anopheles crucians was evaluated in laboratory experiments. first-generation adults reared from field collections illustrated the same trends. individuals reared and maintained under short photoperiod (either 8 h light:16 h dark or 11 h light:13 h dark) lived longer than did those reared and maintained under long photoperiod (either 16 h light:8 h dark or 15 h light:9 h dark). analysis of covariance showed that the greater longev ...19938245940
evaluation of 1-octen-3-ol and carbon dioxide as attractants for mosquitoes associated with irrigated rice fields in arkansas.field studies were conducted to determine the responses of mosquitoes associated with irrigated riceland in arkansas to 1-octen-3-ol (octenol), carbon dioxide (co2) and combinations of both. octenol was released at 4.1 (wick in) and 41.1 (wick out) mg/h and co2 at 200, 500 and 1,000 ml/min. octenol alone did not result in a significant (p greater than 0.05) increased response for any species relative to unbaited traps. an increase in co2 release rate generally resulted in an increase in collecti ...19911680151
defensive consumption of parasitic mites by anopheles crucians larvae. 19883193119
adult body size and parity in field populations of the mosquitoes anopheles crucians, aedes taeniorhynchus and aedes sollicitans. 19873504950
distribution of anopheles quadrimaculatus and an. crucians larvae within rice field habitats in southwestern louisiana.this study, conducted in 3 parishes of louisiana, documented the distribution pattern of anopheles quadrimaculatus and an. crucians during the first crop season. ratios of numbers of 3rd and 4th instar larvae of an. quadrimaculatus to the number of an. crucians 3rd and 4th instar larvae (q/c), varied both spatially and temporally. the mean q/c ratio during 1984 was 1.37; during 1985 it was 1.40. the ratios changed from week to week during each year, and increases or decreases in a particular wee ...19873504942
mortality in mite-infested, male anopheles crucians. 19873504888
the influence of parasitic water mites on the instantaneous death rate of their hosts.the effect of 2 species of water mites on the instantaneous death rate of their hosts was measured on the basis of laboratory experiments. in both parasite-host association - the parasitic water mite hydryphantes tenuabilis on the aquatic insect host hydrometra australis and the parasitic water mite arrenurus pseudotenuicollis on the mosquito anopheles crucians - the effect of mite load on the instantaneous death rate of the host appeared to be linear. also, the impact of a single parasite on th ...197928310464
water mite-induced mortality in a natural popluation of the mosquito anopheles crucians (diptera: culicidae). 1979544826
the effect of a parasitic water mite, arrenurus pseudotenuicollis (acari: hydrachnellae), on the survival and reproduction of the mosquito anopheles crucians (diptera: culicidae). 1977903924
incidence of the fungus coelomomyces punctatus and c. dodgei in larval populations of the mosquito anopheles crucians in two louisiana ponds. 19725014912
the occurrence of anopheles crucians in guatemala. 195114799721
overwintering of anopheles crucians wied. in south carolina. 194918150174
salinity tolerance of anopheles quadrimaculatus and habitat preference of a. crucians bradleyi. 194720264492
hybridization between anopheles crucians and anopheles bradleyi. 197128562948
mosquitoes (diptera: culicidae) collected from residential yards and dog kennels in florida using two aspirators, a sweep net, or a cdc trap.mosquito surveillance typically uses centers for disease control and prevention (cdc) mosquito light traps baited with co2. from january 2013 to march 2015, we sampled seven field sites using three active mosquito-trapping techniques (two different aspirators and a sweep net) and the stationary co2-baited cdc mosquito light trap to determine mosquito capture efficacy for each technique. sampling occurred in four suburban backyards and three dog kennel facilities near gainesville, fl, usa; specie ...201829121250
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