| transporter-mediated uptake of 2-chloro- and 2-hydroxybenzoate by pseudomonas huttiensis strain d1. | we investigated the mechanisms of uptake of 2-chlorobenzoate (2-cba) and 2-hydroxybenzoate (2-hba) by pseudomonas huttiensis strain d1. uptake was monitored by assaying intracellular accumulation of 2-[ul-ring-14c]cba and 2-[ul-ring-14c]hba. uptake of 2-cba showed substrate saturation kinetics with an apparent km of 12.7 +/- 2.6 micromoles and a maximum velocity (vmax) of 9.76 +/- 0.78 nmol min-1 mg of protein-1. enhanced rates of uptake were induced by growth on 2-cba and 2-hba, but not by grow ... | 2003 | 14660391 |
| comparative phylogenies of burkholderia, ralstonia, comamonas, brevundimonas and related organisms derived from rpob, gyrb and rrs gene sequences. | phylogenetic analysis of strains from burkholderia, ralstonia, cupriavidus, comamonas, delftia, acidovorax, brevundimonas, herbaspirillum huttiense and "pseudomonas butanovora" was performed based on the protein-coding genes rpob and gyrb and on the 16s rrna-coding gene rrs. overall, the phylogenies deduced from the three genes were concordant among themselves and with current taxonomy. however, a few differences among individual gene phylogenies were noted. for example, the separation of cupria ... | 2008 | 18280706 |
| proposals of curvibacter gracilis gen. nov., sp. nov. and herbaspirillum putei sp. nov. for bacterial strains isolated from well water and reclassification of [pseudomonas] huttiensis, [pseudomonas] lanceolata, [aquaspirillum] delicatum and [aquaspirillum] autotrophicum as herbaspirillum huttiense comb. nov., curvibacter lanceolatus comb. nov., curvibacter delicatus comb. nov. and herbaspirillum autotrophicum comb. nov. | two strains of curved bacteria, 7-1(t) and 7-2(t), isolated from well water, were phylogenetically examined to determine their taxonomic position. strain 7-1(t) is a gram-negative, slightly curved rod. analysis of the 16s rrna gene sequence showed that strain 7-1(t) formed a cluster with [aquaspirillum] delicatum and [pseudomonas] lanceolata. it has some similar characteristics to [a.] delicatum and [p.] lanceolata, but has sufficient distance to separate it from other genera. dna-dna hybridizat ... | 2004 | 15545462 |
| integration of matrix-assisted laser desorption ionization-time of flight mass spectrometry and molecular cloning for the identification and functional characterization of mobile ortho-halobenzoate oxygenase genes in pseudomonas aeruginosa strain jb2. | protein mass spectrometry and molecular cloning techniques were used to identify and characterize mobile o-halobenzoate oxygenase genes in pseudomonas aeruginosa strain jb2 and pseudomonas huttiensis strain d1. proteins induced in strains jb2 and d1 by growth on 2-chlorobenzoate (2-cba) were extracted from sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels and analyzed by matrix-assisted laser desorption ionization-time of flight mass spectrometry. two bands gave significant matches ... | 2001 | 11722919 |
| cloning, nucleotide sequencing, and functional analysis of a novel, mobile cluster of biodegradation genes from pseudomonas aeruginosa strain jb2. | we have identified in pseudomonas aeruginosa strain jb2 a novel cluster of mobile genes encoding degradation of hydroxy- and halo-aromatic compounds. nineteen open reading frames were located and, based on sequence similarities, were putatively identified as encoding a ring hydroxylating oxygenase (hybabcd), an atp-binding cassette-type transporter, an extradiol ring-cleavage dioxygenase, transcriptional regulatory proteins, enzymes mediating chlorocatechol degradation, and transposition functio ... | 2001 | 11571162 |
| phylogenetic affiliation of the pseudomonads based on 16s rrna sequence. | the broad and vague phenotypic definition allowed the genus pseudomonas to become a dumping ground for incompletely characterized polarly flagellated, gram-negative, rod-shaped, aerobic bacteria, and a large number of species have been accommodated in the genus pseudomonas. the 16s rrna sequences of 128 valid and invalid pseudomonas species, which included almost valid species of the genus pseudomonas listed in the approved lists of bacterial names, were obtained: sequences of 59 species were de ... | 2000 | 10939664 |
| draft genome sequence of herbaspirillum huttiense subsp. putei iam 15032, a strain isolated from well water. | here we report the one-scaffold draft genome of herbaspirillum huttiense subsp. putei strain 7-2(t) (iam 15032), which was isolated from well water. | 2013 | 23469362 |
| severe community-acquired pneumonia with bacteremia caused by herbaspirillum aquaticum or herbaspirillum huttiense in an immune-competent adult. | herbaspirillum spp. are gram-negative bacteria that inhabit soil and water. infections caused by these organisms have been reported in immunocompromised hosts. we describe severe community-acquired pneumonia and bacteremia caused by herbaspirillum aquaticum or h. huttiense in an immunocompetent adult male. | 2015 | 26179298 |
| In vitro evolution of an atrazine-degrading population under cyanuric acid selection pressure: evidence for the selective loss of a 47 kb region on the plasmid ADP1 containing the atzA, B and C genes. | The adaptation of microorganisms to pesticide biodegradation relies on the recruitment of catabolic genes by horizontal gene transfer and homologous recombination mediated by insertion sequences (IS). This environment-friendly function is maintained in the degrading population but it has a cost which could diminish its fitness. The loss of genes in the course of evolution being a major mechanism of ecological specialization, we mimicked evolution in vitro by sub-culturing the atrazine-degrading ... | 2011 | 21959051 |
| diversity analysis of diazotrophic bacteria associated with the roots of tea (camellia sinensis (l.) o. kuntze). | the diversity elucidation by amplified ribosomal dna restriction analysis and 16s rdna sequencing of 96 associative diazotrophs, isolated from the feeder roots of tea on enriched nitrogen-free semisolid media, revealed the predominance of gram-positive over gram-negative bacteria within the kangra valley in himachal pradesh, india. the gram-positive bacteria observed belong to two taxonomic groupings; firmicutes, including the genera bacillus and paenibacillus; and actinobacteria, represented by ... | 2011 | 21715960 |
| reclassification of herbaspirillum putei as a later heterotypic synonym of herbaspirillum huttiense, with the description of h. huttiense subsp. huttiense subsp. nov. and h. huttiense subsp. putei subsp. nov., comb. nov., and description of herbaspirillum aquaticum sp. nov. | resequencing of the 16s rrna gene of the type strain of herbaspirillum putei ding and yokota 2004 revealed 99.9 % sequence similarity to that of the type strain of herbaspirillum huttiense (leifson 1962) ding and yokota 2004. this high phylogenetic relatedness of h. putei and h. huttiense was confirmed by the results of dna-dna hybridization between h. huttiense dsm 10281(t) and h. putei atcc baa-806(t) (reassociation value 96 %). therefore, it is proposed to reclassify the type strain of h. put ... | 2010 | 19671717 |