pcr primers that allow intergeneric differentiation of ascomycetes and their application to verticillium spp. | a pair of conserved pcr primers, designated nms1 and nms2, that amplify a region in the mitochondrial small rrna gene region were designed for fungi belonging to the class ascomycetes. these primers were tested with members of eight fungal genera (aspergillus, fusarium, magnaporthe, mycospharella, neurospora, saccharomyces, sclerotinia, verticillium) and 10 verticillium species (verticillium albo-atrum, verticillium chlamydosporium, verticillium cinnebarium, verticillium dahliae, verticillium fu ... | 1994 | 7811072 |
interactions of human phagocytes with moulds fusarium spp. and verticillium nigrescens possessing different pathogenicity. | fusarium spp. are emerging as important causes of invasive fungal infections. they tend to have decreased susceptibility to antifungal agents, making host defences very important. the ability of human phagocytes to cause damage to hyphae of fusarium solani, f. oxysporum and verticillium nigrescens, a mould with very low pathogenicity, was assessed using the 2,3-bis[2-methoxy-4-nitro-5-sulphophenyl]2h-tetrazolium-5-carboxanilide (xtt) metabolic assay. the oxidative burst, evidenced as superoxide ... | 2003 | 14725324 |
phylogenetic analysis of verticillium species based on nuclear and mitochondrial sequences. | three different genes were sequenced from isolates of five plant-pathogenic verticillium species, verticillium albo-atrum, verticillium dahliae, verticillium longisporum, verticillium nigrescens, and verticillium tricorpus. the sequences covered parts of the mitochondrial cytochrome b gene ( cob), the mitochondrial small subunit rrna gene ( rns) and the nuclear its2 region. when the sequences were combined, the five species clustered in five monophyletic groups, with v. nigrescens distantly rela ... | 2004 | 15138663 |
exploiting composting biodiversity: study of the persistent and biotechnologically relevant microorganisms from lignocellulose-based composting. | the composting ecosystem is a suitable source for the discovery of novel microorganisms and secondary metabolites. this work analyzes the identity of microbial community that persists throughout lignocellulose-based composting, evaluates their metabolic activities and studies the capability of selected isolates for composting bioaugmentation. bacterial species of the phyla firmicutes, actinobacteria and proteobacteria and fungi of the phylum ascomycota were ubiquitous throughout the composting. ... | 2014 | 24759645 |
biodiversity and succession of mycobiota associated to agricultural lignocellulosic waste-based composting. | a comprehensive characterization of the culturable mycobiota associated to all stages of lignocellulose-based composting was achieved. a total of 77 different isolates were detected, 69 of which were identified on the basis of the 5.8-its region sequencing. all the isolates were assigned to the phyla ascomycota and basidiomycota, with prevalence of the sordariomycetes (19) and eurotiomycetes (17) classes. penicillium was the most represented genus (11 species), while the species gibellulopsis ni ... | 2015 | 25863208 |