| annual maturation of paramphistomum cervi (trematoda: paramphistomatidae) in moose, alces alces l. | | 1979 | 540274 |
| [contribution to the biology and pathogenicity of domestic omasal leech paramphistomum cervi. 1. developmental stages in the free state and in the intermediary host]. | | 1977 | 588222 |
| [contribution to the biology and pathogenicity of the endemic rumen flatworm paramphistomum cervi. 2. occurrence in pasture cattle grazing in marshy areas]. | | 1978 | 629734 |
| [biology and pathogenicity of the domestic ruminal fluke paramphistomum cervi. 3. development in cattle, sheep and deer]. | | 1978 | 629739 |
| experimental studies on the suitability of aquatic snails as intermediate hosts for paramphistomum cervi in iraq. | three of the most common species of aquatic snails in iraq were examined for their susceptibility to infection with paramphistomum cervi. bulinus truncatus was the only susceptible species. no development occurred in physa acuta or lymnaea lagotis euphratica. these findings and a previous field observation that infection of b. truncatus with amphistome larvae is not uncommon indicate that b. truncatus is an intermediate host of p. cervi in iraq. young snails of b. truncatus were more susceptible ... | 1978 | 666386 |
| histochemical distribution of acetyl & butyryl cholinesterase in the tissues of a trematode paramphistomum cervi. | | 1978 | 721155 |
| histochemical localization of alkaline phosphatase in the trematode paramphistomum cervi (paramphistomidae). | | 1976 | 992820 |
| parasites of livestock in asir province, southwestern saudi arabia. | thirteen parasite species, three trematodes, five cestodes, one nematode, and four arthropods, have been reported in livestock in asir, southwestern saudi arabia. the occurrence of dicrocoelium dendriticum in imported sheep and paramphistomum cervi in indigenous cattle is recorded for the first time in saudi arabia. | 1990 | 2267729 |
| the susceptibility of lymnaeid snails to paramphistomum cervi infections in mexico. | the occurrence of paramphistomum cervi has been reported in mexico, but its intermediate hosts have not been identified. five species of snails of the genus lymnaea, l. bulimoides, l. columella, l. cubensis, l. humilis and l. palustris, were collected in mexico. only three of the exposed species, l. palustris, l. cubensis and l. humilis, became infected with p. cervi. the first of these snail species was highly susceptible to infection and a higher number of the exposed snails shed cercariae. it ... | 1990 | 2343525 |
| ultrastructure and cytochemistry of the tegument of orthocoelium scoliocoelium and paramphistomum cervi (trematoda: digenea). | the tegument of orthocoelium scoliocoelium and paramphistomum cervi was examined using histochemical techniques and electron microscopy. on the basis of the distribution of acid and alkaline phosphatase (e.c. 3.1.3.2, e.c. 3.1.3.1), non-specific esterase (e.c. 3.1.1.1), cholinesterase (e.c. 3.1.1.7) and succinate dehydrogenase (e.c. 1.3.99.1) at light microscope level two distinct regions were recognized, an outer and an inner zone. electron microscopy revealed that the tegument comprises an out ... | 1988 | 2976778 |
| morphological and histochemical observations on the vitelline cells of developing and adult paramphistomum cervi (trematoda: digenea). | in 6 week-old paramphistomum cervi, vitelline cells appear in the form of a few scattered cells, present below the body wall which divide mitotically to form vitelline follicles in 8 week-old worms. small shell globules appear first in 10 week-old worms, the number and size of which continue to increase till the worm becomes adult. in the latter the vitelline follicles contain stem cells, immature, maturing and mature vitelline cells, which differ due to the distribution of shell globules as wel ... | 1987 | 3437110 |
| histochemical studies on eggshell formation in paramphistomum cervi (digenea: paramphistomatidae). | the formation of the egg mass in the ootype, containing the oocyte, vitelline cells, numerous spermatozoa and mehlis' gland secretion marks the beginning of eggshell formation. mehlis' gland cells pour their lipoprotein secretion into the ootype; this secretion forms a thin template around the egg mass and upon this template the shell globules are deposited. eggshell formation begins in the proximal uterus and is completed in the middle uterus. the eggshells are devoid of phenols and phenolase i ... | 1987 | 3571922 |
| [chaetotaxy of the cercaria of paramphistomum sp., a parasite of cattle in vendée]. | the cercaria was shed by an anisus rotundatus experimentally infected with a parasite of bos taurus in vendée (france). this trematode was identified as paramphistomum cervi. the snail natural host is unknown. the chaetotaxy of the cercaria differs from that of other european species of paramphistomum and particularly from p. leydeni and p. daubneyi both species which occur in vendée. the morphology of the adult precludes any relationship with p. ichikawai also present in vendée. therefore it ve ... | 1987 | 3662332 |
| morphological and histochemical studies on the development of testis and spermatogenesis in the ruminal trematode of sheep, paramphistomum cervi (digenea: paramphistomatidae). | morphological and histochemical changes accompanying testicular development and spermatogenesis have been described in the ruminal trematode paramphistomum cervi during the course of its infection in sheep. small testes of 4-week-old worms contain a few primordial germ cells. spermatogonial cells appear first in 6-week-old worms and increase in number by the 10th week when the testes become large and follicular. mature spermatozoa appear in the testes of 16-week-old worms. general pattern of spe ... | 1986 | 3721355 |
| histochemical alterations in the duodenum of goats experimentally infected with paramphistomum cervi. | certain histochemical alterations in the different tunics of duodenum in kids were studied 20, 40, 60 and 80 days post-infection (dpi) with paramphistomum cervi and the results compared with those of uninfected kids. there was a general reduction of polysaccharide complex substances and glycogen at 20 dpi. a marked decrease in polysaccharide complex substances and glycogen at 20 dpi. a marked decrease in polysaccharide complex substances and glycogen was especially discernible in the brunner's g ... | 1985 | 3992867 |
| [intestinal paramphistomiasis in ruminants. experimental infection of sheep with metacercariae and immature forms of paramphistomum cervi (schrank, 1790)]. | | 1970 | 5530163 |
| morphological and histochemical studies on the ovarian development and oogenesis in paramphistomum cervi (digenea: paramphistomatidae). | the ovary in 4-week-old worms contains undifferentiated germ cells. the oogonial differentiation, together with the appearance of some pas-positive material in the ooplasm occurs in the ovary of 8-week-old worms. the ovary of 16-week-old worms contains only oogonia and oocytes and no germ cells. in adult worms, the oocytes reach the pachytene stage and further development is arrested at this stage. nucleolar fragmentation and subsequent transport of nuclei to the ooplasm have been observed. the ... | 1984 | 6204914 |
| histopathology of the duodenum and rumen of goats during experimental infections with paramphistomum cervi. | on microscopic examination after experimental infection with paramphistomum cervi, tissue reactions in the duodenum were more pronounced during early stages of the infection (20th day post-infection (dpi)). immature parasites were seen migrating to the muscularis layer, and focal infiltration of macrophages and lymphocytes was observed in the lamina propria and in the interstitial tissue of brunner's gland. at places, there was cystic dilatation of brunner's gland. at 40 dpi, the parasite was no ... | 1984 | 6541393 |
| maturation of paramphistomum cervi in sheep in india. | histological studies of the trematode paramphistomum cervi collected throughout the year from the rumen of sheep, revealed the occurrence of gravid worms from april to august and immature worms from september to march. the maturation of the gonads and the accessory sex glands i.e., the prostate-, mehli's- and vitelline-gland began during the period march-april and showed functional peaks during the period july-august, which coincided with the availability of the intermediate host, indoplanorbis ... | 1984 | 6541837 |
| [what is paramphistomum cervi?]. | it is proved histologically that lacking of tegumented papillae in paramphistomum cervi is based on the alteration of the tegument furnished with papillae in the normal state. the differences in the dorsal extension of the vitellaria, in the histological structure of the genital opening complex and in other features, used by eduardo (1982) for the division of p. cervi into 3 species, are refuted as not existing. therefore the forms p. leydeni, p. scotiae and p. hiberniae remain well established ... | 1983 | 6666870 |
| [the incidence of flukes of the genus paramphistomum in czechoslovakia]. | an effort was made to evaluate objectively all findings on the variability of fluke of the family paramphistomatidae and to give a clear survey on the species occurring in this country in order to be able to control it successfully in the case of widespread occurrence of paramphistomatosis or its intermediate hosts. the individual fluke species have specific intermediate hosts and a correct determination can make preventive measurements easier. three species of rumen fluke live parasitically in ... | 1982 | 6814041 |
| effects of carbon dioxide, temperature, ph and the number of worms on the in vitro survival, histology and histochemistry of paramphistomum cervi (paramphistomatidae: digenea). | survival of immature and mature stages of paramphistomum cervi, collected from the intestine and the rumen of sheep has been studied in in vitro using tyrode's, hank's, eagle's, stephenson's saline and hedon--fleig media. twenty-five ml quantities of carbon dioxide (co2)-rich hedon--fleig solution at ph 6.7, at 37 degrees c supported the maximum survival of 5 worms of both immature and mature flukes for periods of 9 and 10 days, respectively. alterations to the ph of the medium, the incubation t ... | 1982 | 6819682 |
| role of oesophageal glands in the digestive physiology of two rumen amphistomes orthocoelium scoliocoelium and paramphistomum cervi. | histochemical techniques have been used to study the chemical composition of the oesophageal gland secretions of orthocoelium scoliocoelium and paramphistomum cervi. results suggest that the secretions contain numerous enzymes, e.g., non-specific alkaline and acid phosphatases, atpase, tppase, esterase (cathepsin c like), beta-galactosidase, beta-glucuronidase and n-acetyl-beta-glucosaminidase. the role of these enzymes in the digestion of food in these amphistomes is discussed. on the basis of ... | 1983 | 6841957 |
| morphological and histochemical observations on the oocapt and oviducal transport of oocytes in paramphistomum cervi (zeder, 1790) (digenea: trematoda). | the oocapt of paramphistomum cervi has a narrow constriction followed by a wide lumen with a thickened wall and is surrounded by several unicellular flask-shaped gland cells opening into the lumen. the oocapt gland cells are filled with granules, the size and number of which vary in the body cells and its duct. histochemical studies have shown that the oocapt gland cells secrete mainly mucoproteins but a few lipoprotein and phospholipid granules have also been observed. the passage of the oocyte ... | 1983 | 6875252 |
| [development of paramphistomum cervi from miracidium to metacercaria]. | | 1981 | 7337647 |
| [the karyotype of paramphistomum cervi (zeder, 1790) from korean cattle] | as a series of systematic classification of paramphistomes, the worms in the rumen and reticulum were collected on 214 korean cattle slaughtered at jeonju abattoir from january 1986 to april 1987 and were classified by means of morphology. afterwards, the karyotype of paramphistomum cervi (zeder, 1790) was detected by means of modified air-drying method from germ cells of the worms. the results were summarized as follows: in the chromosome number of 254 p. cervi, the haploid cell was n=9 and the ... | 1987 | 12886066 |
| studies on transaminase reactions in some parasitic helminths. | by an application of sigma-frankel methods, two transaminase systems, glutamic-pyruvic transaminase and glutamic-oxaloacetic transaminase, were found to operate at a mesurable rate in 2 species of nematodes(ascaris lumbricoides and ascaridia galli), 5 species of trematodes (clonorchis sinensis, fasciola hepatica, eurytrema pancreaticum, paramphistomum cervi and paragonimus westermani) and 5 kinds of cestodes (diphyllobothrium mansoni, dipylidium caninum, taenia pisiformis, cysticercus cellulosae ... | 1966 | 12913567 |
| [metabolism of c(14)-acetate by some trematodes] | the adult trematodes, fasciola hepatica, eurytrema pancreaticum and paramphistomum cervi, employed in this experiment were obtained from the cattle slaughtered at the local abbatoir. the worms selected and washed several times in normal sterilized saline solution. each about ten of intact f. hepatica, fourty of e. pancreaticum, and twenty of p. cervi were incubated in 50 cc volume of special incubation flasks with incubation medium consisting of 10 cc. of krebs-ringer phosphate buffer(ph 7.4) th ... | 1965 | 12913583 |
| [metabolism of c(14)-glucose by paramphistomum cervi] | the trematode paramphistomum cervi empolyed in this experiment was obtained from the reticulum of cattle slaughtered at the local abbatoir. the worms were selected and washed several times in normal sterilized saline solution. each about ten of intact worms were incubated in 50 cc volume of special incubation flasks with incubation mixture consisting of 50 cc of krebs-ringer phosohate buffer (ph 7.4) to which were added universally labeled c(14)-glucose and non-radioactive carrier glucose concen ... | 1965 | 12913589 |
| [infestation of bulinus contortus with cercaria schistosoma bovis (sonsino, 1876) and with paramphistomum cervi (schrank, 1790) in various seasons of the year]. | | 1953 | 13160216 |
| [further inquiries on paramphistomosis (paramphistomum cervi) in sardinia]. | | 1963 | 14123058 |
| seasonal trends of paramphistomum cervi in tabasco, mexico. | thirty rumens were examined every month from may 1989 to april 1992 to establish seasonal trends of infection and their relationship to the climatic factors caused by paramphistomum cervi in cattle. the cattle were chosen from three municipalities of the sierra region in tabasco: jalapa, tacotalpa and teapa. p. cervi was found throughout the year with prevalence between 3.33 and 96.67%, and with a yearly average of 39.10%. the major period of infection was shown in jalapa. however, the maximum m ... | 2003 | 14559164 |
| survey of helminths in adult sheep in heilongjiang province, people's republic of china. | the prevalence of helminths in adult sheep was investigated in heilongjiang province, people's republic of china between january 1999 and september 2003. a total of 326 adult sheep representing local breeds (xingjiang fine wool sheep, dongbei fine wool sheep) as well as introduced breeds (merino and charollais) from representative geographical locations in heilongjiang province were slaughtered and examined for the presence of helminths. the worms were examined, counted and identified to species ... | 2006 | 16713098 |
| natural infection by paramphistomoidea stiles and goldberger, 1910 trematodes in wild marsh deer (blastocerus dichotomus illiger, 1815) from sérgio mottas's hydroelectric power station flooding area. | studies on helminthfauna of marsh deer blastocerus dichotomus illiger, 1815 are rare, although helminthic diseases are an important cause of mortality in these animals. fifteen male and female adult marsh deer from sergio motta's hydroelectric power station flooding area at paraná river which died during the capture and quarantine procedures, between 1998 and 1999, were necropsied. three trematodes species, paramphistomum cervi, balanorchis anastrofus and zygocotyle lunatum, all belonging to sup ... | 2006 | 17196114 |
| paramphistomum cervi: antigenic profile of adults as recognized by infected cattle sera. | an antigenic profile of adult paramphistomum cervi was revealed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) and immunoblotting using sera from cattle naturally infected with p. cervi, fasciola gigantica and strongylids. sds-page of whole worm extracts exhibited 26 distinct protein bands. immunoblotting analysis of these proteins showed five major antigenic bands which were recognized by serum of individual cattle naturally infected with p. cervi. these antigenic prot ... | 2008 | 17892871 |
| laboratory determination of efficacy of indigenous plant extracts for parasites control. | the present study was based on assessments of the antiparasitic activities to determine the efficacies of acetone, chloroform, ethyl acetate, hexane, and methanol dried leaf, flower, and seed extracts of achyranthes aspera l., anisomeles malabarica (l.) r. br., gloriosa superba l., psidium guajava l., ricinus communis l., and solanum trilobatum l. tested against the larvae of cattle tick rhipicephalus (boophilus) microplus (canestrini 1887) (acari: ixodidae), sheep internal parasite paramphistom ... | 2009 | 19308453 |
| adulticidal and larvicidal efficacy of some medicinal plant extracts against tick, fluke and mosquitoes. | the adulticidal and larvicidal effect of indigenous plant extracts were investigated against the adult cattle tick haemaphysalis bispinosa neumann, 1897 (acarina: ixodidae), sheep fluke paramphistomum cervi zeder, 1790 (digenea: paramphistomatidae), fourth instar larvae of malaria vector, anopheles subpictus grassi and japanese encephalitis vector, culex tritaeniorhynchus giles (diptera: culicidae). the aim of this study was to evaluate the toxic effect of leaf hexane, chloroform, ethyl acetate, ... | 2009 | 19819626 |
| the performance of a pcr assay for field studies on the prevalence of fasciola hepatica infection in galba truncatula intermediate host snails. | the aim of this work was to develop a pcr assay for the detection of f. hepatica in galba truncatula snails and to evaluate its performance in field studies. primers were designed to amplify a 124bp non-coding tandem repeat found in the fasciola genome. the result was a banding pattern corresponding to multiples of the initial target sequence. the sensitivity of the pcr was determined on experimentally infected snails. the test was sensitive enough to detect fluke dna in snails experimentally in ... | 2010 | 19939569 |
| paramphistomum cervi: surface topography of the tegument of adult fluke. | adult paramphistomum cervi or rumen fluke are pear-shaped, slightly concave ventrally and convex dorsally. the worm measures about 5-13 mm in length and 2-5 mm in width across the mid-section. as observed by scanning electron microscopy (sem), the tegumental surface in all part of the body, appears highly corrugated with transverse folds alternating with grooves and is spineless. at high magnification, the surface of the fold is composed of microfolds or ridges separated by microgrooves or pits. ... | 2010 | 20045698 |
| evaluation of medicinal plant extracts against blood-sucking parasites. | the present study was based on assessments of the antiparasitic activities to determine the efficacies of acetone, chloroform, ethyl acetate, hexane, and methanol dried leaf, flower, and seed extracts of cassia auriculata l., rhinacanthus nasutus kurz., solanum torvum swartz, terminalia chebula retz., and vitex negundo linn. were tested against larvae of cattle tick rhipicephalus (boophilus) microplus canestrini, 1887 (acari: ixodidae), adult of haemaphysalis bispinosa neumann, 1897 (acarina: ix ... | 2010 | 20306205 |
| fasciola gigantica: production and characterization of a monoclonal antibody against recombinant cathepsin b3. | a number of monoclonal antibodies (moabs) against a recombinant cathepsin b3 (rcatb3) of fasciola gigantica were produced in balb/c mice. reactivity and specificity of these moabs were assessed by indirect elisa and immunoblotting techniques. six stable clones, namely 1c4, 1e9, 2e5, 2f9, 5b4, 5d7 were obtained. all moabs reacted with rcatb3 at molecular weight (mw) 37 kda as well as the glycosylated peptide at 55-75 kda and with the native catb3 at mw 37 kda in wb extracts of metacercariae (met) ... | 2011 | 20736007 |
| major lipids and fatty acids in the liver and rumen fluid of the goat ( capra hircus) infected with the trematode paramphistomum cervi. | the present study records the occurrence of major lipid fractions and their fatty acids in a digenetic trematode parasite paramphistomum cervi, and the rumen fluid and liver of the goat (capra hircus). the amount of neutral lipids (nl), glycolipids (gl) and phospholipids (pl) of goat liver, rumen fluid and of the parasite shows that the rumen fluid is rich in nl, which is also in maximum quantity in the parasite, while the liver is rich in pl followed by nl. the number of fatty acids of total li ... | 2010 | 20831844 |
| evaluation of medicinal plant extracts against ticks and fluke. | the present study was based on assessments of the antiparasitic activities to determine the efficacies of leaf hexane, chloroform, ethyl acetate, acetone and methanol extracts of aegle marmelos (linn.) correa ex roxb, andrographis lineata wallich ex nees., andrographis paniculata (burm.f.) wallich ex nees., cocculus hirsutus (l.) diels, eclipta prostrata l., and tagetes erecta l. against the adult cattle tick haemaphysalis bispinosa neumann 1897 (acarina: ixodidae), the larvae of rhipicephalus ( ... | 2010 | 20922419 |
| characterization and localization of saposin-like protein-2 (sap-2) in fasciola gigantica. | fasciola gigantica saposin-like protein-2 (fgsap-2) belongs to a family of lipid-interacting proteins that are involved in the cytolysis of target cells. in this study, we have cloned and expressed fgsap-2 and produced the antibody against this recombinant protein. rabbit antiserum against rfgsap-2 reacted with a similar native protein in the whole body extracts of the 4-week-old juvenile and adult stage, as well as a protein in their excretion-secretion, but not in the tegument. in situ hybridi ... | 2010 | 21188603 |
| evaluation of medicinal plant extracts and isolated compound epicatechin from ricinus communis against paramphistomum cervi. | the purpose of this study is to determine the efficacies of hexane, chloroform, ethyl acetate, acetone, and methanol leaf extracts of euphorbia hirta l., psidium guajava l., ricinus communis l., solanum trilobatum l., and tridax procumbens l. against sheep fluke paramphistomum cervi (digenea: paramphistomatidae). all plant extracts showed moderate effects after 24-áh of exposure; however, the highest parasite mortality was found in the methanol extract of r. communis. in the present study, bioas ... | 2011 | 21842382 |
| antigenic components, isolation and partial characterization of excretion-secretion fraction of paramphistomum cervi. | the immunogenic components of adult paramphistomum cervi excretion-secretion (es) fraction were revealed by sds-page and immunoblotting technique using sera from cattle naturally infected with p. cervi, fasciola gigantica, strongylids, trichuris sp., and strongyloides sp. by sds-page, it was found that the es fraction comprised 13 distinct protein bands. immunoblotting analysis of these proteins exhibited nine prominent antigenic bands which were recognized by paramphistomosis antisera. these an ... | 2012 | 23266486 |
| comparison of fatty acid contents of the neutral and phospholipids of the trematode paramphistomum cervi and liver of its host, capra hircus. | the present observation records the lipid classes and their fatty acid composition of the neutral lipid and phospholipid of the trematode paramphistomum cervi and liver of its host capra hircus, the common indian goat. thin layer chromatography and gas liquid chromatography were used to identify different neutral lipid and phospholipid components. the results show that among the neutral lipid fractions, the amount of combined hydrocarbon, wax ester and steryl ester is more or less equal in paras ... | 2013 | 24808657 |
| the parasitic fauna of the european bison (bison bonasus) (linnaeus, 1758) and their impact on the conservation. part 1. the summarising list of parasites noted. | during the current century, 88 species of parasites have been recorded in bison bonasus. these are 22 species of protozoa (trypanosoma wrublewskii, t. theileri, giardia sp., sarcocystis cruzi, s. hirsuta, s. hominis, s. fusiformis, neospora caninum, toxoplasma gondii, cryptosporidium sp., eimeria cylindrica, e. subspherica, e. bovis, e. zuernii, e. canadensis, e. ellipsoidalis, e. alabamensis, e. bukidnonensis, e. auburnensis, e. pellita, e. brasiliensis, babesia divergens), 4 trematodes species ... | 2014 | 25119348 |
| production and characterization of a monoclonal antibody against recombinant cathepsin l1 of fasciola gigantica. | monoclonal antibodies (moabs) against a recombinant cathepsin l1 of fasciola gigantica (rfgcatl1) were produced in vitro by fusion of balb/c mice spleen cells immunized with rfgcatl1 and mouse myeloma cells. reactivity and specificity of these moabs were evaluated by indirect elisa and immunoblotting techniques. seven moab clones were selected from the stable hybridoma clones, namely 1e10, 1f5, 3d11, 4b10, 4d3, 4e3 and 5e7. clones 1e10, 1f5 and 3d11 were igm, whereas clones 4b10, 4d3, 4e3 and 5e ... | 2014 | 24736227 |
| identification of the rumen fluke, calicophoron daubneyi, in gb livestock: possible implications for liver fluke diagnosis. | the liver fluke, fasciola hepatica, is common in many parts of great britain. to detect liver fluke infection and to assess whether fasciolicide treatment has been successful, the faecal egg count (fec) and faecal egg count reduction test (fecrt) are widely used. rumen fluke is also increasingly reported from great britain, but its species identity is yet to be determined. liver fluke and rumen fluke eggs are morphologically similar, which may lead to erroneous diagnoses of liver fluke infection ... | 2013 | 23411375 |
| prevalence and intensity of paramphistomum spp. in cattle from south-eastern iran. | parasitological investigations on paramphistomosis were carried out over a 12-month period in the southeast of iran to determine the prevalence and intensity of this disease. | 2017 | 26246825 |
| the neuromuscular system in continuously swimming cercariae from belarus. ii echinostomata, gymnocephala and amphistomata. | the neuromuscular system in cercariae of moliniella anceps, echinostoma revolutum, cathaemasia hians, psilochasmus oxyurus, sphaeridiotrema globulus, paramphistomum cervi and diplodiscus subclavatus was studied with immunocytochemical methods and confocal scanning laser microscopy. the patterns of f-actin in the musculature, 5-ht immunoreactive (ir), fmrfamide-ir neuronal elements and α-tubulin-ir sensory receptors were investigated. the general patterns of musculature, 5-ht- and fmrfamide-ir ne ... | 2012 | 22941527 |
| the mitochondrial genome of paramphistomum cervi (digenea), the first representative for the family paramphistomidae. | we determined the complete mitochondrial dna (mtdna) sequence of a fluke, paramphistomum cervi (digenea: paramphistomidae). this genome (14,014 bp) is slightly larger than that of clonorchis sinensis (13,875 bp), but smaller than those of other digenean species. the mt genome of p. cervi contains 12 protein-coding genes, 22 transfer rna genes, 2 ribosomal rna genes and 2 non-coding regions (ncrs), a complement consistent with those of other digeneans. the arrangement of protein-coding and riboso ... | 2013 | 23990943 |
| cytosolic superoxide dismutase can provide protection against fasciola gigantica. | superoxide dismutases (sod), antioxidant metallo-enzymes, are a part of the first line of defense in the trematode parasites which act as the chief scavengers for reactive oxygen species (ros). a recombinant fasciola gigantica cytosolic sod (fgsod) was expressed in escherichia coli bl21 (de3) and used for immunizing rabbits to obtain polyclonal antibodies (anti-rfgsod). this rabbit anti-rfgsod reacted with the native fgsod at a molecular weight of 17.5kda. the fgsod protein was expressed at high ... | 2016 | 27338185 |
| protection against fasciola gigantica infection in mice by vaccination with recombinant juvenile-specific cathepsin l. | fasciola gigantica cathepsin l1h (fgcatl1h) is one of the major cathepsin l released by juveniles of f. gigantica to aid in the invasion of host's tissues. due to its high sequence similarity with other cathepsin l (catl) isoforms of late stage f. gigantica, it was considered to be a good vaccine candidate that can block all catl-mediated protease activities and affect juveniles as well as adult parasites. in this study, recombinant profgcatl1h protein expressed in yeast, pichia pastoris, system ... | 2015 | 25701317 |
| monoclonal antibody against recombinant fasciola gigantica cathepsin l1h could detect juvenile and adult cathepsin ls of fasciola gigantica. | cathepsin ls (catls), the major cysteine protease secreted by fasciola spp., are important for parasite digestion and tissue invasion. fasciola gigantica cathepsin l1h (fgcatl1h) is the isotype expressed in the early stages for migration and invasion. in the present study, a monoclonal antibody (moab) against recombinant f. gigantica cathepsin l1h (rfgcatl1h) was produced by hybridoma technique using spleen cells from balb/c mice immunized with recombinant profgcatl1h (rprofgcatl1h). this moab i ... | 2015 | 25324133 |
| production and characterization of a monoclonal antibody against recombinant saposin-like protein 2 of fasciola gigantica. | a monoclonal antibody (moab) against recombinant fasciola gigantica saposin-like protein 2 (rfgsap-2) was produced by hybridoma technique using spleen cells from balb/c mice immunized with rfgsap-2. this moab is an igg1, κ light chain isotype. by immunoblotting and indirect elisa, the moab reacted specifically with rfgsap-2, the natural fgsap-2 at 10kda in whole body (wb) and excretory-secretory (es) fractions of f. gigantica. it did not cross react with antigens in wb fractions from other paras ... | 2013 | 23164839 |
| in vitro screening of cymbopogon jwarancusa and conyza canadensis against liver flukes. | aim of present study was to screen medicinal plants for flukicidal activity in vitro to develop alternative sources of treatment for trematodes infection. for this purpose, crude methanolic extracts (cme) of cymbopogn jwarancusa and conyza canadensis were prepared and live adult flukes viz; fasciola gigantica, and paramphistomum cervi isolated from liver and bile ducts of slaughtered buffalo were subjected to different drug concentrations as well as positive and negative control. motility inhibi ... | 2015 | 26695200 |
| diagnosis of fasciola gigantica infection using a monoclonal antibody-based sandwich elisa for detection of circulating cathepsin b3 protease. | a reliable monoclonal antibody (moab)-based sandwich enzyme-linked immunosorbent assay (sandwich elisa) was developed for the detection of circulating cathepsin b3 protease (catb3) in the sera from mice experimentally infected with fasciola gigantica and cattle naturally infected with the same parasite. the moab 2f9 and biotinylated rabbit polyclonal anti-recombinant catb3 antibody were selected due to their high reactivities and specificities to f. gigantica catb3 antigen based on indirect elis ... | 2013 | 23567552 |
| characterization of the complete mitochondrial genome sequence of homalogaster paloniae (gastrodiscidae, trematoda) and comparative analyses with selected digeneans. | gastrodiscidae species are neglected but significant paramphistomes in small ruminants, which can lead to considerable economic losses to the breeding industry of livestock. however, knowledge about molecular ecology, population genetics, and phylogenetic analysis is still limited. in the present study, we firstly sequenced and analyzed the full mitochondrial (mt) genome of homalogaster paloniae (14,490 bp). the gene contents and organization of the h. paloniae mt genome is the same as that of o ... | 2016 | 27300703 |
| the complete mitochondrial genome of gastrothylax crumenifer (gastrothylacidae, trematoda) and comparative analyses with selected trematodes. | in the present study, we sequenced and analyzed the mitochondrial (mt) genome of gastrothylax crumenifer and compared it with other selected trematodes. the full mt genome of g. crumenifer was amplified, sequenced, assembled, analyzed and then subjected to phylogenetic analysis. the complete mt genome of g. crumenifer is 14,801 bp in length and contains two rrna genes, two non-coding regions (lnr and snr), 12 protein-coding genes, and 22 transfer rna genes. the gene organization of the g. crumen ... | 2016 | 27021180 |
| analysis of the complete fischoederius elongatus (paramphistomidae, trematoda) mitochondrial genome. | fischoederius elongates is an important trematode of paramphistomes in ruminants. animals infected with f. elongates often don't show obvious symptoms, so it is easy to be ignored. however it can cause severe economic losses to the breeding industry. knowledge of the mitochondrial genome of f. elongates can be used for phylogenetic and epidemiological studies. | 2015 | 25986221 |
| epidemiological survey of helminths of goats in southern punjab, pakistan. | the main purpose of this study was to investigate the prevalence of helminths of goats such as fasciola hepatica, haemonchus contortus, paramphistomum cervi, oesophagostomum columbian, cotylophoron cotylophorum, monezia expansa, oestertagia oestertagi and oestertagia circumcincta. the overall prevalence of all species of helminthes was 52% in goat. the study was designed to investigate the factors of helminths prevalence on the basis of sex and age of goat with the help of chi-square. all the re ... | 2013 | 23665709 |
| specificity of a coproantigen elisa test for fasciolosis: lack of cross-reactivity with paramphistomum cervi and taenia hydatigena. | a commercial coproantigen elisa test for fasciolosis, based on the use of mm3 monoclonal antibody for antigen capture, was investigated for possible cross-reactivity with paramphistomum cervi, a trematode that commonly infects cattle and sheep grazing in fluke-infested pasture in ireland. histological sections of adult and immature fasciola hepatica and p cervi were incubated with mm3 monoclonal antibody, and its binding to tissue-localised coproantigen was subsequently visualised by immunocytoc ... | 2012 | 23077134 |
| new approach to molecular characterization of paramphistomum cervi and carmyerius gregarius and comparative analyses with selected trematodes. | the two ruminant parasites, paramphistomum cervi and carmyerius gregarius, were collected from fresh-slaughtered native cattle at local abattoirs in sadat district, menoufia province and identified morphologically, then molecularly by sequencing the nucleotides of 18s ribosomal rna gene (18s rrna). the nucleotide sequences of the two isolates were 456 (p. cervi) and 401 bases for (c. gregarius). the data were used along with those of several other helminth species from the genbank to identify th ... | 2016 | 28018999 |
| in-vitro anthelmintic activity of three bangladeshi plants against paramphistomum cervi and haemonchus contortus. | conventional plant-based therapies act as an important therapeutic tool for the treatment of worm infections all over the world and continuous evaluation of medicinal plants to find new potential lead compounds should be carried out. | 2015 | 25868001 |
| bioactivity studies on musa seminifera lour. | musa seminifera lour is a tree-like perennial herb that has been used in folk medicine in bangladesh to heal a number of ailments. | 2013 | 24124283 |
| the complete mitochondrial genome of orthocoelium streptocoelium (digenea: paramphistomidae) for comparison with other digeneans. | orthocoelium streptocoelium is a common paramphistome species parasitizing the rumen and/or reticulum of small ruminants, leading to significant losses. this study first determined the complete mitochondrial (mt) genome of o. streptocoelium. the complete mt genome of o. streptocoelium was amplified, sequenced, assembled, analysed and then compared with those of other digeneans. the entire mt genome of o. streptocoelium is 13,800 bp in length, which is smaller than those of other digeneans except ... | 2017 | 27030161 |
| molecular identification of the rumen flukes paramphistomum leydeni and paramphistomum cervi in a concurrent infection of the red deer cervus elaphus. | paramphistomosis, caused by paramphistomid flukes, is a gastrointestinal parasitic disease of domestic and wild ruminants. originally thought to be limited to the tropics and subtropics, the disease has recently been reported in temperate regions. here we describe the concurrent infection of a red deer doe (cervus elaphus) with paramphistomum leydeni and paramphistomum cervi. this is the first report of p. leydeni in croatia. flukes were identified on the basis of morphological keys (tegumental ... | 2016 | 27813468 |
| transcriptome analysis of the adult rumen fluke paramphistomum cervi following next generation sequencing. | rumen flukes are parasitic trematodes (platyhelminthes: digenea) of major socioeconomic importance in many countries. key representatives, such as paramphistomum cervi, can cause "rumen fluke disease" or paramphistomosis and undermine economic animal productivity and welfare. p. cervi is primarily a problem in sheep, goat and buffalo production as a consequence of reduced weight gain and milk production, clinical disease or death. recent technological advances in genomics and bioinformatics now ... | 2015 | 26049095 |
| mitochondrial and nuclear ribosomal dna dataset supports that paramphistomum leydeni (trematoda: digenea) is a distinct rumen fluke species. | rumen flukes parasitize the rumen and reticulum of ruminants, causing paramphistomiasis. over the years, there has been considerable debate as to whether paramphistomum leydeni and paramphistomum cervi are the same or distant species. | 2015 | 25890015 |
| morphological and histological identification of paramphistomum cervi (trematoda: paramiphistoma) in the rumen of infected sheep. | this study was undertaken to identify paramphistomum cervi on the basis of its morphology and histology to be the common cause of paramphistomosis in infected sheep and its differentiation from other similar paramphistomes in gujarat. | 2015 | 27047009 |
| characterization of the complete nuclear ribosomal dna sequences of paramphistomum cervi. | sequences of the complete nuclear ribosomal dna (rdna) gene from five individual paramphistomum cervi were determined for the first time. the five complete rdna sequences, which included the 18s rdna, the internal transcribed spacer 1 (its1), the 5.8s rdna, the internal transcribed spacer 2 (its2), the 28s rdna, and the intergenic spacer (igs) regions, had a length range of 8,493-10,221 bp. the lengths of the investigated 18s, its1, 5.8s, its2, and 28s rdna sequences, which were 1,994 bp, 1,293 ... | 2014 | 25140347 |
| regulatory role of adrenergic neurotransmitters on the spontaneous muscular activity in the ruminant trematode paramphistomum cervi (paramphistomatidae). | the neuromuscular system of helminths is an important area for target identification and drug development. many anthelmintics, namely ivermectin, levamisole, piperazine, pyrantel, praziquantel and organophosphates, produce paralysis of helminths by affecting their neuromuscular systems. the neuromuscular system of helminths is also an important area of research to identify some of the important differences between the neuromuscular physiology of helminths and mammals. the identification of diffe ... | 2014 | 23721888 |
| paramphistomum cervi: the in vitro effect of plumbagin on motility, survival and tegument structure. | paramphistomiasis causes enteritis and anemia in livestocks and result in substantial production and economic losses. it is considered a neglected tropical disease, with no effective trematodicidal compound for treatment. plumbagin (pb), a compound founds to be rich in the roots of plumbago indica, is a naphthoquinone derivatives which can induce oxidative stress in parasites. in this study we have evaluated the anthelmintic activity of pb against adult paramphistomum cervi by incubating the par ... | 2013 | 23206952 |