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identification and molecular characterization of a new nonsegmented double-stranded rna virus isolated from culex mosquitoes in japan.two infectious agents were isolated from culex species mosquitoes in japan and were identified as distinct strains of a new rna virus by a method for sequence-independent amplification of viral nucleic acids. the virus designated omono river virus (omrv) replicated in mosquito cells in which it produced a severe cytopathic effect. icosahedral virus particles of approximately 40 nm in diameter were detected in the cytoplasm of infected cells. the omrv genome was observed to consist of a nonsegmen ...201020875466
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