| the stress granule component g3bp is a novel interaction partner for the nuclear shuttle proteins of the nanovirus pea necrotic yellow dwarf virus and geminivirus abutilon mosaic virus. | stress granules (sgs) are structures within cells that regulate gene expression during stress response, e.g. viral infection. in mammalian cells assembly of sgs is dependent on the ras-gap sh3-domain-binding protein (g3bp). the c-terminal domain of the viral nonstructural protein 3 (nsp3) of semliki forest virus (sfv) forms a complex with mammalian g3bp and sequesters it into viral rna replication complexes in a manner that inhibits the formation of sgs. the binding domain of nsp3 to hsg3bp was ... | 2017 | 27693920 |
| genome diversity and evidence of recombination and reassortment in nanoviruses from europe. | the recent identification of a new nanovirus, pea necrotic yellow dwarf virus, from pea in germany prompted us to survey wild and cultivated legumes for nanovirus infections in several european countries. this led to the identification of two new nanoviruses: black medic leaf roll virus (bmlrv) and pea yellow stunt virus (pysv), each considered a putative new species. the complete genomes of a pysv isolate from austria and three bmlrv isolates from austria, azerbaijan and sweden were sequenced. ... | 2014 | 24515973 |
| analyses of pea necrotic yellow dwarf virus-encoded proteins. | pea necrotic yellow dwarf virus (pnydv) is a multipartite, circular, single-stranded dna plant virus. pnydv encodes eight proteins and the function of three of which remains unknown-u1, u2, and u4. pnydv proteins cellular localization was analyzed by gfp tagging and bimolecular fluorescence complementation (bifc) studies. the interactions of all eight pnydv proteins were tested pairwise in planta (36 combinations in total). seven interactions were identified and two (m-rep with cp and mp with u4 ... | 2017 | 28238159 |