| a ribonuclease from phoma cucurbitacearum. | | 1968 | 4968769 |
| Molecular characterization of boscalid- and penthiopyrad-resistant isolates of Didymella bryoniae and assessment of their sensitivity to fluopyram. | BACKGROUND: Didymella bryoniae has a history of developing resistance to single-site fungicides. A recent example is with the succinate-dehydrogenase-inhibiting fungicide (SDHI) boscalid. In laboratory assays, out of 103 isolates of this fungus, 82 and seven were found to be very highly resistant (B(VHR) ) and highly resistant (B(HR) ) to boscalid respectively. Cross-resistance studies with the new SDHI penthiopyrad showed that the B(VHR) isolates were only highly resistant to penthiopyrad (B(V ... | 2011 | 22076736 |
| [isolation and identification of an endophytic fungus of polygonatum cyrtonema and its antifungal metabolites]. | endophytic fungi in medicinal plants produce a variety of bioactivity compounds. in this study, an endophytic fungus zjqy610 with antifungal activity was isolated from polygonatum cyrtonema in zhejiang qingyuan baishanzu mountain nature reserve. | 2010 | 20931871 |
| sensitivity to azoxystrobin in didymella bryoniae isolates collected before and after field use of strobilurin fungicides. | isolates of didymella bryoniae (auersw.) rehm, causal agent of gummy stem blight on cucurbits, developed insensitivity to azoxystrobin in the eastern united states 2 years after first commercial use in 1998. baseline sensitivity of this fungus to azoxystrobin has never been reported. the objectives were to compare baseline sensitivities of d. bryoniae from south carolina and other locations to sensitivities of isolates exposed to azoxystrobin for one or more seasons, and to compare sensitivity i ... | 2009 | 19488995 |
| rhizobacterially induced protection of watermelon against didymella bryoniae. | to identify rhizobacteria from the mekong delta of vietnam, which can systemically protect watermelon against didymella bryoniae and elucidate the mechanisms involved in the protection conferred by isolate pseudomonas aeruginosa 23(1-1). | 2010 | 20163499 |
| [transformation of didymella bryoniae mediated by agrobacterium tumefaciens]. | gummy stem blight, a plant disease caused by didymella bryoniae, is one of the major diseases in melon. the disease can seriously reduce melon yield and quality. however, little information is available on the genetics and functional genomics of the fungal pathogen. in this study, we developed an agrobacterium-mediated transformation system for d. bryoniae by using a universal pathogenic isolate db11 and the agrobacterium tumefaciens strain c58c1 carrying plasmid pbig2rhph2 harboring the hygromy ... | 2010 | 20815261 |
| microbial diversity inside pumpkins: microhabitat-specific communities display a high antagonistic potential against phytopathogens. | recent and substantial yield losses of styrian oil pumpkin (cucurbita pepo l. subsp. pepo var. styriaca greb.) are primarily caused by the ascomycetous fungus didymella bryoniae but bacterial pathogens are frequently involved as well. the diversity of endophytic microbial communities from seeds (spermosphere), roots (endorhiza), flowers (anthosphere), and fruits (carposphere) of three different pumpkin cultivars was studied to develop a biocontrol strategy. a multiphasic approach combining molec ... | 2011 | 21947430 |
| fungal pathogenic nucleic acid detection achieved with a microfluidic microarray device. | detection of polymerase chain reaction (pcr) products obtained from cultured greenhouse fungal pathogens, botrytis cinerea and didymella bryoniae has been achieved using a previously developed microfluidic microarray assembly (mma) device. the flexible probe construction and rapid dna detection resulted from the use of centrifugal pumping in the steps of probe introduction and sample delivery, respectively. the line arrays of the oligonucleotide probes were "printed" on a cd-like glass chip usin ... | 2008 | 18267145 |
| simultaneous detection of acidovorax avenae subsp. citrulli and didymella bryoniae in cucurbit seedlots using magnetic capture hybridization and real-time polymerase chain reaction. | to improve the simultaneous detection of two pathogens in cucurbit seed, a combination of magnetic capture hybridization (mch) and multiplex real-time polymerase chain reaction (pcr) was developed. single-stranded dna hybridization capture probes targeting dna of acidovorax avenae subsp. citrulli, causal agent of bacterial fruit blotch, and didymella bryoniae, causal agent of gummy stem blight, were covalently attached to magnetic particles and used to selectively concentrate template dna from c ... | 2009 | 19453225 |
| internal transcribed spacer regions 1 and 2 and random amplified polymorphic dna analysis of didymella bryoniae and related phoma species isolated from cucurbits. | abstract didymella bryoniae (anamorph phoma cucurbitacearum) is the causal agent of gummy stem blight, although other phoma species are often isolated from cucurbit plants exhibiting symptoms of the disease. the molecular and phylogenetic relationships between d. bryoniae and these phoma species are unknown. isolates of d. bryoniae and phoma obtained from cucurbits grown at various geographical locations in the united states were subjected to random amplified polymorphic dna (rapd) analysis and ... | 2002 | 18944025 |
| fungitoxicity of lyophilized and spray-dried garlic extracts. | among the compounds discussed for anti-microbial and anti-fungal use allicin (allylthiosulfinate, diallyl disulfide-s-monoxide), an active ingredient of garlic, has attracted considerable attention. the objective of this study is to determine the antifungal activity of a local garlic ecotype (voghiera) extracts against different pathogens. primary screening was carried out by the agar plates technique using ethanol garlic extract at four final concentrations against the following organisms: alte ... | 2007 | 17763036 |
| clavatol and patulin formation as the antagonistic principle of aspergillus clavatonanicus, an endophytic fungus of taxus mairei. | many endophytic fungi are known to protect plants from plant pathogens, but the antagonistic mechanism has rarely been revealed. in this study, we wished to learn whether an endophytic aspergillus sp., isolated from taxus mairei, would indeed produce bioactive components, and if so whether (a) they would antagonize plant pathogenic fungi; and (b) whether this aspergillus sp. would produce the compound also under conditions of confrontation with these fungi. the endophytic fungal strain from t. m ... | 2008 | 18317751 |
| aflp analysis of a worldwide collection of didymella bryoniae. | didymella bryoniae (anamorph phoma cucurbitacearum) is an ascomycete that causes gummy stem blight, a foliar disease that occurs on cucurbits in greenhouses and fields throughout the world. in a previous study using rapd analysis, little genetic diversity was found among isolates of d. bryoniae from new york and south carolina, usa. here we report the use of amplified fragment length polymorphism (aflp) analysis to assess the genetic variation within a worldwide collection of d. bryoniae, 102 fi ... | 2003 | 12825498 |
| flexible microarray construction and fast dna hybridization conducted on a microfluidic chip for greenhouse plant fungal pathogen detection. | this study employed a microfluidic method in which probe creation does not require pin-spotting and fast hybridization is conducted on the same microarray chip for the detection of three greenhouse pathogens ( botrytis cinerea, didymella bryoniae, and botrytis squamosa). in this method, 16 oligonucleotide probe line arrays were created on a glass substrate by a microfluidic printing method. then, low amounts of the dna samples (1 fmol of oligonucelotides or 1.4 ng of pcr products) were introduce ... | 2007 | 18047274 |
| five independent loci each control monogenic resistance to gummy stem blight in melon ( cucumis melo l.). | inheritance and segregation analysis demonstrated that five independent genes in melon confer monogenic resistance to foliar infection by the fungal pathogen didymella bryoniae, resulting in the disease known as gummy stem blight (gsb). in this study, two new monogenic sources of gsb resistance were characterized. resistance in cucumis melo pi 482398 was monogenic dominant based on segregation analysis of f(1), f(2) and backcross populations, while resistance in c. melo pi 482399 showed monogeni ... | 2004 | 15067389 |
| evaluation of thin films of agarose on glass for hybridization of dna to identify plant pathogens with microarray technology. | agarose-coated glass slides, after activation, were spotted with amine-modified oligonucleotide probes using a manual eight-pin arraying device. two probes, designed to identify two common greenhouse fungal plant pathogens, didymella bryoniae and botrytis cinerea, were hybridized with polymerase chain reaction (pcr)-amplified fluorescently labeled dna extracted from pure culture and from diseased plant tissue. the probes easily distinguished these pathogens from each other without cross reaction ... | 2005 | 15958185 |
| isolation, antifungal activity, and structure elucidation of the glutarimide antibiotic, streptimidone, produced by micromonospora coerulea. | the antibiotic ao58a,which showed strong antifungal activity against some plant pathogenic fungi, was purified from the culture broth and mycelial mats of micromonospora coerulea strain ao58 using various chromatographic procedures. the molecular formula of the antibiotic ao58a was deduced to be c(16)h(23)no(4) (m + h, m/z 294.1707) by high-resolution fab mass spectroscopy. analyses of (1)h nmr, (13)c nmr, and 2d nmr spectral data revealed that the antibiotic ao58a is the glutarimide antibiotic ... | 1999 | 10552660 |