differentiation of the "ascochyta complex" fungi of pea by biochemical and molecular markers.isolates of three closely related pea pathogens, ascochyta pisi, ascochyta pinodes (teleomorph mycosphaerella pinodes) and phoma medicaginis var. pinodella, were compared by means of isozyme analysis and restriction fragment length polymorphisms (rflp) of amplified rdna spacers. three enzyme systems differentiated a. pisi from m. pinodes and p. m. pinodella. the internal transcribed spacers (itss) of the three fungi showed no intraspecific and very little interspecific variation after digestion ...19968821666
characterization of pisatin-inducible cytochrome p450s in fungal pathogens of pea that detoxify the pea phytoalexin pisatin.many fungi that are pathogenic on pea have the ability to demethylate and thus detoxify the pea phytoalexin pisatin. this detoxification reaction has been studied most thoroughly in nectria haematococca mp vi where it functions as a virulence trait. the enzyme catalyzing this reaction [pisatin demethylase (pda)] is a cytochrome p450. in the current study, the induction of whole-cell pda activity and the biochemical properties of pda in microsomal preparations from the pea pathogens ascochyta pis ...200111407884
a diffusible factor from arbuscular mycorrhizal fungi induces symbiosis-specific mtenod11 expression in roots of medicago truncatula.using dual cultures of arbuscular mycorrhizal (am) fungi and medicago truncatula separated by a physical barrier, we demonstrate that hyphae from germinating spores produce a diffusible factor that is perceived by roots in the absence of direct physical contact. this am factor elicits expression of the nod factor-inducible gene mtenod11, visualized using a pmtenod11-gusa reporter. transgene induction occurs primarily in the root cortex, with expression stretching from the zone of root hair emerg ...200312644648
molecular relatedness within the "ascochyta pinodes-complex".the morphological, molecular and pathological characters of 34 isolates identified as ascochyta pinodes, a. phaseolorum, and phoma medicaginis var. pinodella were determined. initial molecular findings, based on pcr fragment size and rflps of sections of the mitochondrial genome, the rrna gene cluster and the beta-tubulin gene complex, were further investigated by sequencing of the total rrna its regions. isolates were homogenous for all characteristics determined, with the exception of conidial ...200314682458
taxonomical re-evaluation of phoma-like soybean pathogenic fungi.coelomycetous fungi classified in ascochyta, phoma, and phyllosticta have been recorded from spots on leaves and pods of soybeans. based on the genealogical concordance phylogenetic species concept, the authors suggest the re-evaluation of the taxonomic status of phoma sojicola (syn.=ascochyta sojicola) and phyllosticta sojicola. in spite of the former delimitation of phoma sojicola based on small differences in morphological features, it has proved to be identical to phoma pinodella. similarly, ...200919049869
a new species of phoma causes ascochyta blight symptoms on field peas (pisum sativum) in south australia.phoma koolunga sp. nov. is described, having been isolated from ascochyta blight lesions on field pea (pisum sativum) in south australia. the species is described morphologically and sequences of the internal transcribed spacer region compared with those of the accepted pathogens causing ascochyta blight of field peas. p. koolunga was distinct from mycosphaerella pinodes (anamorph: ascochyta pinodes), phoma medicaginis var. pinodella and ascochyta pisi. under controlled conditions the symptoms o ...200919271674
analysis of the mating-type loci of co-occurring and phylogenetically related species of ascochyta and phoma.ascochyta and phoma are fungal genera containing several important plant pathogenic species. these genera are morphologically similar, and recent molecular studies performed to unravel their phylogeny have resulted in the establishment of several new genera within the newly erected didymellaceae family. an analysis of the structure of fungal mating-type genes can contribute to a better understanding of the taxonomic relationships of these plant pathogens, and may shed some light on their evoluti ...201122014305
comparison of cultural growth and in planta quantification of didymella pinodes, phoma koolunga and phoma medicaginis var. pinodella, causal agents of ascochyta blight on field pea (pisum sativum).the causal agents of ascochyta blight on field pea in south australia, didymella pinodes, phoma medicaginis var. pinodella and phoma koolunga, are isolated from a single plant within a crop, suggesting competition for space and nutrients. interactions among these pathogens were investigated. diameters of colonies of d. pinodes and of p. medicaginis var. pinodella were significantly reduced on pda amended with filtrate from broth cultures of p. koolunga as were diameters of colonies of d. pinodes ...201121933927
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