the structure and physiological activity of a glycoprotein secreted from conidia of mycosphaerella pinodes. ii. 19873594654
differentiation of the "ascochyta complex" fungi of pea by biochemical and molecular markers.isolates of three closely related pea pathogens, ascochyta pisi, ascochyta pinodes (teleomorph mycosphaerella pinodes) and phoma medicaginis var. pinodella, were compared by means of isozyme analysis and restriction fragment length polymorphisms (rflp) of amplified rdna spacers. three enzyme systems differentiated a. pisi from m. pinodes and p. m. pinodella. the internal transcribed spacers (itss) of the three fungi showed no intraspecific and very little interspecific variation after digestion ...19968821666
[the developmental process of mycosphaerella pinodes (berk. et blox.) stone. i. the formation of reproductive organs in normal cultures]. 195313159245
[the developmental process of mycosphaerella pinodes (berk. et blox.) stone. ii. effect of hydrogen ion concentration on development of reproductive organs]. 195313159252
biological control of pathogens causing root rot complex in field pea using clonostachys rosea strain acm941.abstract pea root rot complex (prrc), caused by alternaria alternata, aphanomyces euteiches, fusarium oxysporum f. sp. pisi, f. solani f. sp. pisi, mycosphaerella pinodes, pythium spp., rhizoctonia solani, and sclerotinia sclerotiorum, is a major yield-limiting factor for field pea production in canada. a strain of clonostachys rosea (syn. gliocladium roseum), acm941 (atcc 74447), was identified as a mycoparasite against these pathogens. when grown near the pathogen, acm941 often was stimulated ...200318944343
comparison of cultural growth and in planta quantification of didymella pinodes, phoma koolunga and phoma medicaginis var. pinodella, causal agents of ascochyta blight on field pea (pisum sativum).the causal agents of ascochyta blight on field pea in south australia, didymella pinodes, phoma medicaginis var. pinodella and phoma koolunga, are isolated from a single plant within a crop, suggesting competition for space and nutrients. interactions among these pathogens were investigated. diameters of colonies of d. pinodes and of p. medicaginis var. pinodella were significantly reduced on pda amended with filtrate from broth cultures of p. koolunga as were diameters of colonies of d. pinodes ...201121933927
analysis of the mating-type loci of co-occurring and phylogenetically related species of ascochyta and phoma.ascochyta and phoma are fungal genera containing several important plant pathogenic species. these genera are morphologically similar, and recent molecular studies performed to unravel their phylogeny have resulted in the establishment of several new genera within the newly erected didymellaceae family. an analysis of the structure of fungal mating-type genes can contribute to a better understanding of the taxonomic relationships of these plant pathogens, and may shed some light on their evoluti ...201122014305
two-dimensional electrophoresis based proteomic analysis of the pea (pisum sativum) in response to mycosphaerella pinodes.responses to mycosphaerella pinodes in pea were studied by using a proteomics approach. two-dimensional electrophoresis (2-de) was used in order to compare the leaf proteome of two pea cultivars displaying different phenotypes (susceptible and partial resistance to the fungus), as well as in response to the inoculation. multivariate statistical analysis identified 84 differential protein spots under the experimental conditions (cultivars/treatments). all of these 84 protein spots were subjected ...201021090568
superoxide generation in extracts from isolated plant cell walls is regulated by fungal signal molecules.abstract fractions solubilized with nacl from cell walls of pea and cowpea plants catalyzed the formation of blue formazan from nitroblue tetrazolium. because superoxide dismutase decreased formazan production by over 90%, superoxide anion (o(2) ) may participate in the formation of formazan in the solubilized cell wall fractions. the formazan formation in the fractions solubilized from pea and cowpea cell walls was markedly reduced by exclusion of nad(p)h, manganese ion, or p-coumaric acid from ...199718945053
mapping of quantitative trait loci for partial resistance to mycosphaerella pinodes in pea (pisum sativum l.), at the seedling and adult plant stages.the inheritance of resistance to ascochyta blight, an economically important foliar disease of field pea ( pisum sativum l.) worldwide, was investigated. breeding resistant pea varieties to this disease, caused by mycosphaerella pinodes, is difficult due to the availability of only partial resistance. we mapped and characterized quantitative trait loci (qtls) for resistance to m. pinodes in pea. a population of 135 recombinant inbred lines (rils), derived from the cross between dp (partially res ...200414968300
expression of the 12-oxophytodienoic acid 10,11-reductase gene in the compatible interaction between pea and fungal pathogen.suppressors produced by mycosphaerella pinodes are glycopeptides to block pea defense responses induced by elicitors. a clone, s64, was isolated as cdna for suppressor-inducible gene from pea epicotyls. the treatment of pea epicotyls with suppressor alone induced an increase of s64 mrna within 1 h, and it reached a maximum level at 3 h after treatment. the induction was not affected by application of the elicitor, indicating that the suppressor has a dominant action to regulate s64 gene expressi ...200212407201
characterization of pisatin-inducible cytochrome p450s in fungal pathogens of pea that detoxify the pea phytoalexin pisatin.many fungi that are pathogenic on pea have the ability to demethylate and thus detoxify the pea phytoalexin pisatin. this detoxification reaction has been studied most thoroughly in nectria haematococca mp vi where it functions as a virulence trait. the enzyme catalyzing this reaction [pisatin demethylase (pda)] is a cytochrome p450. in the current study, the induction of whole-cell pda activity and the biochemical properties of pda in microsomal preparations from the pea pathogens ascochyta pis ...200111407884
inhibition of atpase activity in pea plasma membranes by fungal suppressors from mycosphaerella pinodes and their peptide moieties.the effects of two suppressors of the defense reactions of host plants, which had been purified from the pea pathogen mycosphaerella pinodes, as well as the effects of peptide moieties, on the atpase activity in pea plasma membranes were examined in vitro. one of the suppressors, supprescin b, inhibited the atpase activity in a non-competitive manner, but the other suppressor, supprescin a, did not. supprescin a was observed to reduce the inhibitory effect of supprescin b. a tripeptide, ser-ser- ...19938019782
a new species of phoma causes ascochyta blight symptoms on field peas (pisum sativum) in south australia.phoma koolunga sp. nov. is described, having been isolated from ascochyta blight lesions on field pea (pisum sativum) in south australia. the species is described morphologically and sequences of the internal transcribed spacer region compared with those of the accepted pathogens causing ascochyta blight of field peas. p. koolunga was distinct from mycosphaerella pinodes (anamorph: ascochyta pinodes), phoma medicaginis var. pinodella and ascochyta pisi. under controlled conditions the symptoms o ...200919271674
candidate genes for quantitative resistance to mycosphaerella pinodes in pea (pisum sativum l.).partial resistance to mycosphaerella pinodes in pea is quantitatively inherited. genomic regions involved in resistance (qtls) have been previously identified in the pea genome, but the molecular basis of the resistance is still unknown. the objective of this study was to map resistance gene analogs (rga) and defense-related (dr) genes in the ji296 x dp ril population that has been used for mapping qtls for resistance to m. pinodes, and identify co-localizations between candidate genes and qtls. ...200717265025
structure and expression of 12-oxophytodienoate reductase (subgroup i) genes in pea, and characterization of the oxidoreductase activities of their recombinant products.recently, we observed that expression of a pea gene (s64) encoding an oxophytodienoic acid reductase (opr) was induced by a suppressor of pea defense responses, secreted by the pea pathogen mycosphaerella pinodes. because it is known that oprs are usually encoded by families of homologous genes, we screened for genomic and cdna clones encoding members of this putative opr family in pea. we isolated five members of the opr gene family from a pea genomic dna library, and amplified six cdna clones, ...200414727182
induction of defense responses by synthetic glycopeptides that have a partial structure of the elicitor in the spore germination fluid of mycosphaerella pinodes.a high molecular weight elicitor (> 70 kda) from spore germination fluid of a pea pathogen, mycosphaerella pinodes, has a partial structure of beta-d-glc-(1-->6)-alpha-d-man-(1-->6)-d-man, which is o-glycosidically attached to serine in the protein moiety. to elucidate the minimum structure for the elicitor activity to pea plants, the effects of nine glycopeptides including beta-d-glc-(1-->6)-alpha-d-man-(1-->6)-d-man-o-ser (no. 1) to [beta-d-glc-(1-->6)-alpha-d-man-(1-->6)-d-man]3-o-ser3-pro3 ( ...199910588068
association between ion fluxes and defense responses in pea and cowpea tissues.the glycopeptide elicitor from a pea pathogen, mycosphaerella pinodes, induced rapid alkalinization and increases in levels of na+ and k+ ions in the extracellular solution upon contact with pea and cowpea tissues. the presence of monensin, nigericin, lidocaine, quinidine or phenytoin together with the elicitor markedly inhibited these changes, whereas the presence of valinomycin, gramicidin d, tetraethylammonium, cscl and aminopyridine did not. the production of phytoalexins in pea and cowpea t ...19979249987
functional analysis of the promoters of phenylalanine ammonia-lyase genes in pea.pycnospore germination fluid of mycosphaerella pinodes, a fungus pathogenic on pea, contains both an elicitor and a suppressor of the accumulation of pisatin, a major phytoalexin of pea. transcription of the genes encoding key enzymes in the biosynthesis of pisatin, namely pal (a gene encoding phenylalanine ammonia-lyase) and chs (a gene encoding chalcone synthase), was shown to be activated upon the treatment of pea epicotyl tissues with the fungal elicitor and suppressed upon treatment with th ...19947981963
structure and physiological activity of conidial polysaccharides of mycosphaerella pinodes. i. 19863791502
identification of genes differentially expressed in a resistant reaction to mycosphaerella pinodes in pea using microarray technology.ascochyta blight, caused by mycosphaerella pinodes is one of the most important pea pathogens. however, little is known about the genes and mechanisms of resistance acting against m. pinodes in pea. resistance identified so far to this pathogen is incomplete, polygenic and scarce in pea, being most common in pisum relatives. the identification of the genes underlying resistance would increase our knowledge about m. pinodes-pea interaction and would facilitate the introgression of resistance into ...201121226971
evolutionary relationships among ascochyta species infecting wild and cultivated hosts in the legume tribes cicereae and vicieae.evolutionary relationships were inferred among a worldwide sample of ascochyta fungi from wild and cultivated legume hosts based on phylogenetic analyses of dna sequences from the ribosomal internal transcribed spacer regions (its), as well as portions of three protein-coding genes: glyceraldehyde-3-phosphate-dehydrogenase (g3pd), translation elongation factor 1-alpha (ef) and chitin synthase 1 (chs). all legume-associated ascochyta species had nearly identical its sequences and clustered with o ...200717663124
construction of a linkage map based on a lathyrus sativus backcross population and preliminary investigation of qtls associated with resistance to ascochyta blight.a linkage map of the lathyrus sativus genome was constructed using 92 backcross individuals derived from a cross between an accession resistant (atc 80878) to ascochyta blight caused by mycosphaerella pinodes and a susceptible accession (atc 80407). a total of 64 markers were mapped on the backcross population, including 47 rapd, seven sequence-tagged microsatellite site and 13 sts/caps markers. the map comprised nine linkage groups, covered a map distance of 803.1 cm, and the average spacing be ...200415502913
characterization of the metabolite produced by mycosphaerella pinodes, the causal agent of mycosphaerella blight on field peas (pisum sativum l.).the metabolite produced by mycosphaerella pinodes, the causal agent of mycosphaerella blight on field peas, was detected by thin layer chromatography (tlc) and was analyzed for its chemical and pathogenic characteristics. one blue dot was detected using 254nm uv light on tlc plate, and a spray of rho-anisaldehyde (110 degrees c, 30 min) also produced a blue dot. the solvent systems used for tlc analysis were ethyl acetate/water/acetone (5/2/5), chloroform/methanol/glacial acetic acid (19/10/2), ...200415462518
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