| purification and characterization of an extracellular beta-glucosidase from the wood-grown fungus xylaria regalis | xylaria regalis, a wood-grown ascomycete isolated in taiwan, produces beta-glucosidase (ec 3.2.1.21) extracellularly. the beta-glucosidase was purified to homogeneity by ammonium sulfate precipitation, ion-exchange, and gel filtration chromatography. the molecular mass of the purified enzyme was estimated to be 85 kda by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. with p-nitrophenyl beta-d-glucopyranoside (pnpg) as the substrate at ph 5.0 and 50°c, the km was 1.72 mm and vmax ... | 1996 | 8875909 |