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transmission, characterization, and serology of a luteovirus associated with yellow leaf syndrome of sugarcane.abstract a previously uncharacterized luteovirus was associated with one form of yellow leaf syndrome (yls), a widespread disease of sugarcane (saccharum sp.). the virus was named sugarcane yellow leaf luteovirus (scylv), and was identified in major sugarcane-producing areas of the world. typical disease symptoms were reproduced when scylv was transmitted by melanaphis sacchari or rhopalosiphum maidis from infected to healthy sugarcane, suggesting that this virus may be the causal agent of one f ...200018944599
categorizing sugarcane cultivar resistance to the sugarcane aphid and yellow sugarcane aphid (hemiptera: aphididae).sugarcane (saccharum spp.) in louisiana is colonized by two aphid species, the sugarcane aphid, melanaphis sacchari (zehntner), and the yellow sugarcane aphid, sipha flava (forbes) (hemiptera: aphididae). the main problem associated with m. sacchari is transmission of sugarcane yellow leaf virus, a casual agent of yellow leaf disease whose absence has been added to certification standards for micropropagated sugarcane in louisiana. greenhouse studies were conducted to categorize dominant commerc ...201020857758
permanent genetic resources added to molecular ecology resources database 1 june 2010 - 31 july 2010.this article documents the addition of 205 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: bagassa guianensis, bulweria bulwerii, camelus bactrianus, chaenogobius annularis, creontiades dilutus, diachasmimorpha tryoni, dioscorea alata, euhrychiopsis lecontei, gmelina arborea, haliotis discus hannai, hirtella physophora, melanaphis sacchari, munida isos, thaumastocoris peregrinus and tuberolachnus salignus. these loci were cro ...201021565125
cloning and expression analysis of cinnamoyl-coa reductase (ccr) genes in sorghum.cinnamoyl-coa reductase (ccr) is the first enzyme in the monolignol-specific branch of the lignin biosynthetic pathway. in this research, three sorghum ccr genes including sbccr1, sbccr2-1 and sbccr2-2 were cloned and characterized. analyses of the structure and phylogeny of the three ccr genes showed evolutionary conservation of the functional domains and divergence of function. transient expression assays in nicotiana benthamiana leaves demonstrated that the three ccr proteins were localized i ...201627231650
quantification of sugarcane yellow leaf virus in sugarcane following transmission through aphid vector, melanaphis sacchari.yellow leaf caused by sugarcane yellow leaf virus (scylv) is a serious constraint to sugarcane production in india and currently the disease epidemics occur on many of the susceptible varieties under field conditions. studies were conducted on the virus transmission by sugarcane aphid melanaphis sacchari in sugarcane by inoculating virus-free meristem derived from micro- propagated plants of sugarcane cv co 86032 with viruliferous aphids. virus transmission was confirmed through rt-pcr assays an ...201526645033
putative recombination signature and significance of insertion/deletion events in the rna-dependent rna polymerase coding region of sugarcane yellow leaf virus.the 5898 nucleotide single-strand rna genome of sugarcane yellow leaf virus (scylv) contains one long open reading frame, which is translated into a 120.6 kda polyprotein. the sequences of scylv isolates from the two scylv-susceptible cultivars from hawaii had a deletion of 48-51 nt in orf1. scylv from 12 sugarcane hybrid cultivars from different origins were tested by rt-pcr using a specific set of primers, to investigate the genome segment for this deletion. only three cultivars were found not ...201222542996
relative toxicity of two aphicides to hippodamia convergens (coleoptera: coccinellidae): implications for integrated management of sugarcane aphid, melanaphis sacchari (hemiptera: aphididae).flupyradifurone and sulfoxaflor present novel insecticide chemistries with particular efficacy against aphids, and the recent emergence of sugarcane aphid, melanaphis sacchari (zehntner), as a pest of sorghum in the united states has resulted in their widespread use. we examined their toxicity to hippodamia convergens guerin-meneville, an important aphid biocontrol agent. we exposed beetles to topical applications of the field rate (fr) of these insecticides, fed them contaminated food (eggs of ...201728039423
indigenous aphid predators show high levels of preadaptation to a novel prey, melanaphis sacchari (hemiptera: aphididae).the performance of four aphid predators, hippodamia convergens guerin-meneville, coleomegilla maculata degeer, chrysoperla carnea stephens and orius insidiosus say was compared on three prey species: schizaphis graminum rondani, melanaphis sacchari (zehntner), and ephestia kuehniella zeller eggs. species predatory in both life stages (all except ch. carnea) were reared on e. kuehniella eggs and switched to aphid prey for assessment of reproduction. differences were greater between the e. kuehnie ...201526470381
sugarcane aphid (hemiptera: aphididae): host range and sorghum resistance including cross-resistance from greenbug sources.the graminous host range and sources of sorghum [sorghum bicolor (l.) moench.] plant resistance, including cross-resistance from greenbug, schizaphis graminum (rondani), were studied for the newly emerging sugarcane aphid, melanaphis sacchari (zehntner), in greenhouse no-choice experiments and field evaluations. the sugarcane aphid could not survive on field corn, zea mays (l.), teff grass, eragrostis tef (zucc.), proso millet, panicum miliaceum l., barley, hordeum vulgare l., and rye, secale ce ...201526470168
sugarcane aphid population growth, plant injury, and natural enemies on selected grain sorghum hybrids in texas and louisiana.in response to the 2013 outbreak of sugarcane aphid, melanaphis sacchari (zehntner) (hemiptera: aphididae), on sorghum, sorghum bicolor (l.), in north america, experiments were conducted at three southern u.s. grain sorghum production locations (corpus christi, tx; winnsboro, la; rosenberg, tx). the objectives were to authenticate yield decline on susceptible hybrids (2014 and 2015) and to measure aphid population growth and natural enemy prevalence on susceptible and resistant hybrids with simi ...201728962004
differences in flight activity of coleomegilla maculata and hippodamia convergens (coleoptera: coccinellidae) following emergence, mating, and reproduction.the flight activity of coleomegilla maculata degeer and hippodamia convergens guerin-meneville (coleoptera: coccinellidae) was examined by observing tethered beetles in the laboratory. c. maculata were fed eggs of ephestia kuehniella zeller, as were larval h. convergens, whereas adult h. convergens were fed melanaphis sacchari (zehntner) to induce egg maturation; adults of both species received water and diluted honey. a spot of magnetic paint was applied to one elytrum of each beetle, which the ...201728961806
sugarcane aphid (hemiptera: aphididae): a new pest on sorghum in north america.in 2013, the sugarcane aphid, melanaphis sacchari (zehntner) (hemiptera: aphididae), a new invasive pest of sorghum species in north america, was confirmed on sorghum in 4 states and 38 counties in the united states. in 2015, the aphid was reported on sorghum in 17 states and over 400 counties as well as all sorghum-producing regions in mexico. ability to overwinter on living annual and perennial hosts in southern sorghum-producing areas and wind-aided movement of alate aphids appear to be the m ...201628446991
toxicity of three aphicides to the generalist predators chrysoperla carnea (neuroptera: chrysopidae) and orius insidiosus (hemiptera: anthocoridae).recent widespread infestations of the invasive sugarcane aphid, melanaphis sacchari (zehntner) (hemiptera: aphididae), in sorghum fields in the southern usa have created demand for insecticides that will provide effective control of sugarcane aphid, while conserving those beneficial species that contribute to biological control of the pest. we tested the susceptibility of both adult and immature stages of two aphid predators, the green lacewing, chrysoperla carnea (stephens) (neuroptera: chrysop ...201728357620
feeding by melanaphis sacchari (hemiptera: aphididae) facilitates use of sorghum by rhopalosiphum padi (hemiptera: aphididae), but reciprocal effects are negative.in the process of feeding, aphids modify the physiology of their host plants in species-specific ways, and plant responses to these aggressions are often genotype- or cultivar-specific. thus, different aphid species sharing a host can influence each other's fitness via plant-mediated interactions, usually with negative and asymmetric impacts. here, we show that feeding by the sugarcane aphid, melanaphis sacchari zehntner, can improve the suitability of sorghum as a host plant for the bird cherry ...201728073905
resistance to melanaphis sacchari (hemiptera: aphididae) in forage and grain sorghums.the sugarcane aphid, melanaphis sacchari (zehntner), has established itself as a perennial pest of grain and forage sorghums in the united states since the summer of 2013. we conducted traditional plant resistance studies that determine tolerance, antibiosis, and antixenosis in 32 sorghum genotypes when challenged with sugarcane aphids. the genotypes included one exotic plant introduction and 31 seed and pollinator parental lines that are used to produce grain, sudangrass, and forage sorghum hyb ...201728011682
host plant specialization in the sugarcane aphid melanaphis sacchari.most aphids are highly specialized on one or two related plant species and generalist species often include sympatric populations adapted to different host plants. our aim was to test the hypothesis of the existence of host specialized lineages of the aphid melanaphis sacchari in reunion island. to this end, we investigated the genetic diversity of the aphid and its association with host plants by analyzing the effect of wild sorghum sorghum bicolor subsp. verticilliflorum or sugarcane as host p ...201526600253
ndvi to detect sugarcane aphid injury to grain sorghum.multispectral remote sensing has potential to provide quick and inexpensive information on sugarcane aphid, melanaphis sacchari (zehntner), pest status in sorghum fields. we describe a study conducted to determine if injury caused by sugarcane aphid to sorghum plants in fields of grain sorghum could be detected using multispectral remote sensing from a fixed wing aircraft. a study was conducted in commercial grain sorghum fields in the texas gulf coast region in june 2014. twenty-six commercial ...201526470276
comparative life histories of greenbugs and sugarcane aphids (hemiptera: aphididae) coinfesting susceptible and resistant sorghums.host-plant resistance has been a fundamental component of aphid management in cereal crops. over decades, various sources of resistance to greenbug, schizaphis graminum (rondani), were bred into cultivars of sorghum, sorghum bicolor (l.) moench, to counter recurring virulent greenbug biotypes. the recent invasion of sugarcane aphid, melanaphis sacchari (zehntner), raised questions about plant-mediated interactions between the two aphids and the possibility of using greenbug antibiosis against su ...201626357844
low genetic diversity in melanaphis sacchari aphid populations at the worldwide scale.numerous studies have examined the genetic diversity and genetic structure of invading species, with contrasting results concerning the relative roles of genetic diversity and phenotypic plasticity in the success of introduced populations. increasing evidence shows that asexual lineages of aphids are able to occupy a wide geographical and ecological range of habitats despite low genetic diversity. the anholocyclic aphid melanaphis sacchari is a pest of sugarcane and sorghum which originated in t ...201425148510
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