| the development of onchocerca volvulus in two temperate blackfly species, simulium ornatum meigen and simulium lineatum meigen. | in view of the promise shown by temperate simuliids for laboratory colonization, the ability of simulium ornatum and s. lineatum to support the development of onchocerca volvulus was investigated. an injection technique is described for the introduction of o. volvulus microfilariae from a chimpanzee into female simuliids in the absence of a bloodmeal. rpmi 1640 medium, with penicillin, streptomycin and foetal calf serum, proved satisfactory as an inoculation medium. third stage larvae were obtai ... | 1979 | 539857 |
| acquired resistance to onchocerca lienalis infections in simulium ornatum meigen and simulium lineatum meigen following passive transfer of haemolymph from previously infected simuliids (diptera, simuliidae). | this study describes experiments which demonstrate the presence of a parasite-induced or derived factor in the haemocoel of onchocerca lienalis-infected simuliids. haemolymph, when transferred from previously infected donor flies to previously untreated recipient flies, using fine glass needles, confers protection against a subsequent challenge to the recipients. in three trials using simulium ornatum sl. or s. lineatum, significant levels of protection in 'immunized' compared to 'challenge cont ... | 1986 | 3714299 |
| genetic diversity and identification of palearctic black flies in the subgenus wilhelmia (diptera: simuliidae). | accurate species identifications are the essential first step in understanding the medical, economic, and ecological importance of black flies. the utility of dna barcoding based on cytochrome c oxidase subunit 1 (coi) sequences was evaluated for identifying six common species of palearctic black flies in the subgenus wilhelmia, including several that are virulent pests. chromosomally identified larvae from turkey and germany and coi sequences in genbank were analyzed. intraspecific genetic dive ... | 2017 | 28399227 |
| bioamines and proteins in the saliva and salivary glands of palaearctic blackflies (diptera: simuliidae). | to elucidate the mechanisms of skin-reactions to blackfly bites, components in the saliva and salivary glands of simulium lineatum and s. equinum females were studied. histamine, putrescine, spermine, n1-monoacetyl-spermine, and spermidine were detected in both the saliva and salivary gland extract through high-performance liquid chromatography (hplc), the content of histamine being much higher than that of the other amines. whereas histamine concentration was increased by food stimulation and a ... | 1990 | 2339249 |