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vibrio owensii sp. nov., isolated from cultured crustaceans in australia.two bacterial strains (dy05(t) and 47666-1) were isolated in queensland, australia, from diseased cultured crustaceans panulirus ornatus and penaeus monodon, respectively. on the basis of 16s rrna gene sequence identity, the strains were shown to belong to the harveyi clade of the genus vibrio. multilocus sequence analysis using five housekeeping genes (rpoa, pyrh, topa, ftsz and mreb) showed that the strains form a monophyletic group with 94.4% concatenated sequence identity to the closest spec ...201020002183
Vibrio jasicida sp. nov., a member of the Harveyi clade, from marine animals (packhorse lobster, abalone, and Atlantic salmon).Six isolates of a facultatively anaerobic bacterium were recovered in culture from marine invertebrates and vertebrates, including packhorse lobster (Jasus verreauxi), abalone (Haliotis sp.) and Atlantic salmon (Salmo salar) between 1994 and 2002. The bacteria are Gram-negative, rod shaped and motile by means of more than one polar flagellum, oxidase-positive, catalase-positive and able to grow between 0.5-8.0% NaCl (optimum 3.0-6.0%) and at temperatures of 10-37 °C (optimum 25-30 °C). On the ba ...201121984666
probiont niche specialization contributes to additive protection against vibrio owensii in spiny lobster larvae.the development of efficient probiotic application protocols for use in marine larviculture relies on comprehensive understanding of pathogen-probiont-host interactions. the probiont combination of pseudoalteromonas sp. pp107 and vibrio sp. pp05 provides additive protection against vectored vibrio owensii dy05 infection in larvae (phyllosomas) of ornate spiny lobster, panulirus ornatus. here, fluorescently tagged strains were used to demonstrate niche specialization of these probionts in both th ...201223757129
screening of marine bacteria with bacteriocin-like activities and probiotic potential for ornate spiny lobster (panulirus ornatus) juveniles.bacteriocins are ribosomally synthesized antimicrobial peptides, which have been found in diverse bacterial species of terrestrial origins and some from the sea. new bacteriocins with new characteristics, new origins and new applications are likely still awaiting discovery. the present study screened bacteria isolated from marine animals of interest to the aquaculture industry for antimicrobial and bacteriocin-like activities in order to uncover biodiversity of bacteriocin producers, and explore ...201424969424
taxonomic revision of harveyi clade bacteria (family vibrionaceae) based on analysis of whole genome sequences.use of inadequate methods for classification of bacteria in the so-called harveyi clade (family vibrionaceae, gammaproteobacteria) has led to incorrect assignment of strains and proliferation of synonymous species. in order to resolve taxonomic ambiguities within the harveyi clade and to test usefulness of whole genome sequence data for classification of vibrionaceae, draft genome sequences of 12 strains were determined and analysed. the sequencing included type strains of seven species: vibrio ...201323710045
unique and conserved genome regions in vibrio harveyi and related species in comparison with the shrimp pathogen vibrio harveyi caim 1792.vibrio harveyi caim 1792 is a marine bacterial strain that causes mortality in farmed shrimp in north-west mexico, and the identification of virulence genes in this strain is important for understanding its pathogenicity. the aim of this work was to compare the v. harveyi caim 1792 genome with related genome sequences to determine their phylogenic relationship and explore unique regions in silico that differentiate this strain from other v. harveyi strains. twenty-one newly sequenced genomes wer ...201526198743
virulence changes to harveyi clade bacteria infected with bacteriophage from vibrio owensii.vibrio owensii is one of the most virulent vibrios known being able to kill crustacean larvae at 10(2) cfu ml(-1). this study describes virulence changes to naïve strains of vibrio harveyi and vibrio campbellii when infected with the bacteriophage vob from a closely related species v. owensii 47666-1. the bacteriophage from v. owensii was induced into lytic phase by using mitomycin c at 100 ng ml(-1). one strain of v. harveyi and two strains of v. campbellii from 29 tested containing no prophage ...201324426274
vibrio inhibens sp. nov., a novel bacterium with inhibitory activity against vibrio species.strain bflp-10(t), isolated from faeces of wild long-snouted seahorses (hippocampus guttulatus), is a gram-negative, motile and facultatively anaerobic rod. this bacterium produces inhibitory activity against vibrio species. phylogenetic analysis based on 16s rrna gene sequences showed that strain bflp-10(t) was a member of the genus vibrio and was most closely related to vibrio owensii (99%), vibrio communis (98.9%), vibrio sagamiensis (98.9%) and vibrio rotiferianus (98.4%). however, multilocu ...201222472571
rapid diagnosis of vibrio owensii responsible for shrimp acute hepatopancreatic necrosis disease with isothermal recombinase polymerase amplification assay.a rapid and sensitive ahpnd-rpa assay was developed for the specific detection of the ahpnd-causing vibrio owensii. the ahpnd-rpa detected as few as 2 copies per reaction in 9.02 ± 0.66 min at 39 °c, and showed 100% positive predictive value and negative predictive value for ahpnd diagnosis.201728179103
draft genome sequence of vibrio owensii strain sh-14, which causes shrimp acute hepatopancreatic necrosis disease.we sequenced vibrio owensii strain sh-14, which causes serious acute hepatopancreatic necrosis disease (ahpnd) in shrimp. sequence analysis showed a large extrachromosomal plasmid, which encoded pir toxin genes and shared highly sequence similarity with the one observed in ahpnd-causing vibrio parahaemolyticus strains. the results suggest that this plasmid appears to play an important role in shrimp ahpnd.201526634753
draft genome sequence of vibrio owensii gra50-12, isolated from green algae in the intertidal zone of eastern taiwan.vibrio owensii gra50-12 was isolated from symbiotic green algae of coral. the genome contains genes encoding toxin production, virulence regulation, stress response proteins, types ii, iv, and vi secretion systems, and proteins for the metabolism of aromatic compounds, which reflects its pathogenic potential and its ecological roles in the ocean.201525593265
isolation and characterization of five lytic bacteriophages infecting a vibrio strain closely related to vibrio owensii.vibrio owensii is a potential bacterial pathogen in marine aquaculture system. in this study, five lytic phages specific against vibrio strain b8d, closely related to v. owensii, were isolated from seawater of an abalone farm. the phages were characterized with respect to morphology, genome size, growth phenotype, as well as thermal, and ph stability. all phages were found to belong to the family siphoviridae with long noncontractile tails and terminal fibers. restriction analysis indicated that ...201324102707
vibrio owensii induces the tissue loss disease montipora white syndrome in the hawaiian reef coral montipora capitata.incidences of coral disease in the indo-pacific are increasing at an alarming rate. in particular, montipora white syndrome, a tissue-loss disease found on corals throughout the hawaiian archipelago, has the potential to degrade hawaii's reefs. to identify the etiologic agent of montipora white syndrome, bacteria were isolated from a diseased fragment of montipora capitata and used in a screen for virulent strains. a single isolate, designated strain ocn002, recreated disease signs in 53% of cor ...201223056419
identification of an antagonistic probiotic combination protecting ornate spiny lobster (panulirus ornatus) larvae against vibrio owensii infection.vibrio owensii dy05 is a serious pathogen causing epizootics in the larviculture of ornate spiny lobster panulirus ornatus. in the present study a multi-tiered probiotic screening strategy was used to identify a probiotic combination capable of protecting p. ornatus larvae (phyllosomas) from experimental v. owensii dy05 infection. from a pool of more than 500 marine bacterial isolates, 91 showed definitive in vitro antagonistic activity towards the pathogen. antagonistic candidates were shortlis ...201222792184
pathogenicity and infection cycle of vibrio owensii in larviculture of the ornate spiny lobster (panulirus ornatus).the type strain of vibrio owensii (dy05) was isolated during an epizootic of aquaculture-reared larvae (phyllosomas) of the ornate spiny lobster (panulirus ornatus). v. owensii dy05 was formally demonstrated to be the etiological agent of a disease causing rapid and reproducible larval mortality with pathologies similar to those seen during disease epizootics. vectored challenge via the aquaculture live feed organism artemia (brine shrimp) caused consistent cumulative mortality rates of 84 to 89 ...201222307306
bacterial communities associated with healthy and acropora white syndrome-affected corals from american samoa.acropora white syndrome (aws) is characterized by rapid tissue loss revealing the white underlying skeleton and affects corals worldwide; however, reports of causal agents are conflicting. samples were collected from healthy and diseased corals and seawater around american samoa and bacteria associated with aws characterized using both culture-dependent and culture-independent methods, from coral mucus and tissue slurries, respectively. bacterial 16s rrna gene clone libraries derived from coral ...201222283330
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