| a distinct tospovirus causing necrotic streak on alstroemeria sp. in colombia. | a tospovirus causing necrotic streaks on leaves was isolated from alstroemeria sp. in colombia. infected samples reacted positively with tomato spotted wilt virus (tswv) antiserum during preliminary serological tests. further analysis revealed a close serological relationship to tomato chlorotic spot virus (tcsv) and groundnut ringspot virus (grsv). a major part of the s-rna segment, encompassing the nucleocapsid (n) protein gene, the 5' untranslated region and a part of the intergenic region 3' ... | 2010 | 20151164 |
| development of a microarray for simultaneous detection and differentiation of different tospoviruses that are serologically related to tomato spotted wilt virus. | tospoviruses, the plant-infecting genus in the family bunyaviridae, are thrips borne and cause severe agricultural losses worldwide. based on the serological relationships of the structural nucleocapsid protein (np), the current tospoviruses are divided into six serogroups. the use of np-antisera is convenient for virus detection, but it is insufficient to identify virus species grouped in a serogroup due to the serological cross-reaction. alternatively, virus species can be identified by the n ... | 2017 | 28081705 |
| genetic and serological characterization of chrysanthemum stem necrosis virus, a member of the genus tospovirus. | chrysanthemum stem necrosis virus (csnv) is a member of a tentative tospovirus species. in this study, the complete genomic sequence of the japanese csnv isolate tcch07a was determined. the l rna is 8960 nt long and encodes the 331.0-kda rna-dependent rna polymerase. the m rna is 4828 nt long and encodes the 34.1-kda movement protein (nsm) and the 127.7-kda glycoprotein precursor (gn/gc). the s rna is 2949 nt long and encodes the 52.4-kda silencing suppressor protein (nss) and the 29.3-kda nucle ... | 2015 | 25427981 |
| the nsm proteins of phylogenetically related tospoviruses trigger sw-5b-mediated resistance dissociated of their cell-to-cell movement function. | the cell-to-cell movement protein (nsm) of tomato spotted wilt virus (tswv) has been recently identified as the effector of the single dominant sw-5b resistance gene from tomato (solanum lycopersicum l.). although most tswv isolates shows a resistance-inducing (ri) phenotype, regular reports have appeared on the emergence of resistance-breaking (rb) isolates in tomato fields, and suggested a strong association with two point mutations (c118y and t120n) in the nsm protein. in this study the sw-5b ... | 2017 | 28754561 |