| characterization of antigens of paragonimus miyazakii by elisa. | the characterization of adult worm and excretory-secretory (es) antigens of p. miyazakii was performed with a sandwich enzyme-linked immunosorbent assay (elisa) igg and fab' fragments were prepared from p. miyazakii-infected rat sera. the former was purified by affinity column chromatography. the latter was prepared from igg2c by passage through a protein a affinity column, then conjugated to horseradish peroxidase. cross-reactivity of the elisa with fasciola hepatica. fischoederius elongatus. t ... | 1989 | 2772708 |
| fascioliasis and other plant-borne trematode zoonoses. | fascioliasis and other food-borne trematodiases are included in the list of important helminthiases with a great impact on human development. six plant-borne trematode species have been found to affect humans: fasciola hepatica, fasciola gigantica and fasciolopsis buski (fasciolidae), gastrodiscoides hominis (gastrodiscidae), watsonius watsoni and fischoederius elongatus (paramphistomidae). whereas f. hepatica and f. gigantica are hepatic, the other four species are intestinal parasites. the fas ... | 2005 | 16150452 |
| high annealing temperature-random amplified polymorphic dna (hat-rapd) analysis of three paramphistome flukes from thailand. | the rumen flukes of 37 cows (bos indicus) from chiang mai and lamphun provinces were investigated, and the overall prevalence of infection was 78.38% (29/37). three species were found: paramphistomum epiclitum, orthocoelium streptocoelium, and fischoederius elongatus with prevalences of infection of 75.68%, 48.65%, and 40.54%, respectively. genomic dna was amplified by polymerase chain reaction based on the high annealing temperature-random amplification of polymorphic dna (hat-rapd) technique. ... | 2007 | 16889772 |
| the complete mitochondrial genome of gastrothylax crumenifer (gastrothylacidae, trematoda) and comparative analyses with selected trematodes. | in the present study, we sequenced and analyzed the mitochondrial (mt) genome of gastrothylax crumenifer and compared it with other selected trematodes. the full mt genome of g. crumenifer was amplified, sequenced, assembled, analyzed and then subjected to phylogenetic analysis. the complete mt genome of g. crumenifer is 14,801 bp in length and contains two rrna genes, two non-coding regions (lnr and snr), 12 protein-coding genes, and 22 transfer rna genes. the gene organization of the g. crumen ... | 2016 | 27021180 |
| analysis of the complete fischoederius elongatus (paramphistomidae, trematoda) mitochondrial genome. | fischoederius elongates is an important trematode of paramphistomes in ruminants. animals infected with f. elongates often don't show obvious symptoms, so it is easy to be ignored. however it can cause severe economic losses to the breeding industry. knowledge of the mitochondrial genome of f. elongates can be used for phylogenetic and epidemiological studies. | 2015 | 25986221 |
| ultrastructure and histochemistry of the tegument of juvenile paramphistomes during migration in indian ruminants. | the tegument of juvenile paramphistomum epiclitum and fischoederius elongatus (paramphistomidae: digenea) resembles those of other digeneans. seven types of papillae were observed, mostly on the oral and acetabular surfaces, and increase in number during migration. also evident are two types of secretory body (t1 and t2) which are synthesized separately in tegumental cytons underlying the syncytium. exocytosis of t2 bodies occurs at the apical membrane and appears to contribute to a fibrous glyc ... | 1994 | 7829842 |
| ultrastructure and histochemistry of the lymph system and parenchyma of juvenile paramphistomes (paramphistomidae: digenea) during migration in indian ruminants. | the lymph system of juvenile paramphistomum epiclitum and fischoederius elongatus consists of a single pair of longitudinal primary vessels from which sub-dividing branches extend laterally to associate with most major tissues and organs. the system originates shortly after excystation in the definitive host and is fully developed in day 14 juveniles. lymph vessels are syncytial and membrane limited, with a matrix which contains autophagic-like inclusions, clusters of ser and free nuclei. simila ... | 1992 | 1487371 |
| ultrastructure and histochemistry of the protonephridial system of juvenile paramphistomum epiclitum and fischoederius elongatus (paramphistomidae: digenea) during migration in indian ruminants. | the protonephridial system of juvenile paramphistomum epiclitum and fischoederius elongatus consists of a bilaterally symmetrical arrangement of primary, secondary and tertiary ducts which connect individual flame cells with a simple common bladder. primary and secondary ducts are formed from columns of adjoining cells which provide an epithelial lining, whose luminal surface is elaborated with either short tubercles or lamellae. groups of cilia project from the luminal surface at frequent inter ... | 1992 | 1487370 |
| dna quantities and qualities from various stages of some trematodes using optical and hat-rapd methods. | the aim of this experiment was to minimize dna quantity and quality for detection by optical (spectrophotometer at 260 nm and 280 nm) and hat-rapd methods. total dna from different stages, adult, metacercaria and eggs of 6 trematode species were isolated for analysis. in this experiment, the adult trematodes were classified into 3 groups by size: small, haplorchis taichui and stellantchasmus falcatus; medium, opisthorchis viverrini and ganeo tigrinus; and large, paramphistomum epiclitum and fisc ... | 2006 | 17547055 |
| copro-pcr based detection of bovine schistosome infection in india. | schistosomosis and amphistomosis are the two economically important and widely prevalent snail-borne trematode infections in grazing cattle of southern india. acute infections are symptomatically similar and difficult to detect by routine microscopy for eggs. the present study was directed towards the development of a copro-polymerase chain reaction (copro-pcr) for detection of bovine schistosome species, using custom-designed primers targeting 18s and 28s ribosomal rna as well as mitochondrial ... | 2016 | 26693890 |
| prevalence and sequence-based identity of rumen fluke in cattle and deer in new caledonia. | an abattoir survey was performed in the french melanesian archipelago of new caledonia to determine the prevalence of paramphistomes in cattle and deer and to generate material for molecular typing at species and subspecies level. prevalence in adult cattle was high at animal level (70% of 387 adult cattle) and batch level (81%). prevalence was lower in calves at both levels (33% of 484 calves, 51% at batch level). animals from 2 of 7 deer farms were positive for rumen fluke, with animal-level p ... | 2016 | 27043709 |