| comparison of antemortem and environmental samples for zebrafish health monitoring and quarantine. | molecular diagnostic assays offer both exquisite sensitivity and the ability to test a wide variety of sample types. various types of environmental sample, such as detritus and concentrated water, might provide a useful adjunct to sentinels in routine zebrafish health monitoring. similarly, antemortem sampling would be advantageous for expediting zebrafish quarantine, without euthanasia of valuable fish. we evaluated the detection of mycobacterium chelonae, m. fortuitum, m. peregrinum, pseudocap ... | 2017 | 28724491 |
| ultrastructure and molecular phylogeny of pleistophora hyphessobryconis (microsporidia) infecting hybrid jundiara (leiarius marmoratus × pseudoplatystoma reticulatum) in a brazilian aquaculture facility. | a microsporidian infecting the skeletal muscle of hybrid jundiara (leiarius marmoratus × pseudoplatystoma reticulatum) in a commercial aquaculture facility in brazil is described. affected fish exhibited massive infections in the skeletal muscle that were characterized by large opaque foci throughout the affected fillets. histologically, skeletal muscle was replaced by inflammatory cells and masses of microsporidial developmental stages. generally pyriform spores had a wrinkled bi-layer spore wa ... | 2016 | 26522338 |
| ultrastructure and molecular phylogenetics of a new isolate of pleistophora pagri sp. nov. (microsporidia, pleistophoridae) from pagrus pagrus in egypt. | the spore morphology and molecular systematic of a new microsporidian which was isolated from the common sea bream pagrus pagrus (f: sparidae linnaeus, 1758) from the red sea, egypt have been studied. fifty-six out of 300 (18.7%) of this fish were infected with microsporidian parasites. the infection was appeared as whitish, ellipsoid, round, or elongated nodules embedded in the epithelial lining of the peritoneum and also in the intestinal epithelium. light microscopic study revealed that nodul ... | 2012 | 22773009 |
| microsporidiosis in zebrafish research facilities. | pseudoloma neurophilia (microsporidia) is the most common pathogen detected in zebrafish (danio rerio) from research facilities. the parasite infects the central nervous system and muscle and may be associated with emaciation and skeletal deformities. however, many fish exhibit subclinical infections. another microsporidium, pleistophora hyphessobryconis, has recently been detected in a few zebrafish facilities. here, we review the methods for diagnosis and detection, modes of transmission, and ... | 2012 | 23382342 |
| Luna stain, an improved selective stain for detection of microsporidian spores in histologic sections. | Microsporidia in histologic sections are most often diagnosed by observing spores in host tissues. Spores are easy to identify if they occur in large aggregates or xenomas when sections are stained with hematoxylin and eosin (H&E). However, individual spores are not frequently detected in host tissues with conventional H&E staining, particularly if spores are scattered within the tissues, areas of inflammation, or small spores in nuclei (i.e. Nucleospora salmonis). Hence, a variety of selective ... | 2011 | 21848126 |
| pleistophora hyphessobryconis (microsporidia) infecting zebrafish danio rerio in research facilities. | zebrafish danio rerio are important models for biomedical research, and thus, there is an increased concern about diseases afflicting them. here we describe infections by pleistophora hyphessobryconis (microsporidia) in zebrafish from 3 laboratories. as reported in other aquarium fishes, affected zebrafish exhibited massive infections in the skeletal muscle, with no involvement of smooth or cardiac muscle. in addition, numerous spores within macrophages were observed in the visceral organs, incl ... | 2010 | 20853741 |
| ultrastructure, development, and molecular phylogeny of pleistophora hyphessobryconis, a broad host microsporidian parasite of puntius tetrazona. | a potentially fatal microsporidial infection targeting the skeletal muscles of the tiger barb puntius tetrazona was described. ultrastructural and molecular analyses of infected tissues confirmed that the causative parasite was pleistophora hyphessobryconis. compared to p. hyphessobryconis observed in other hosts, those infecting tiger barb demonstrated differences in ultrastructure that may be related to host adaptation. phylogenetic analysis revealed that classifications based on different met ... | 2012 | 22773044 |
| unusual fluorescent granulomas and myonecrosis in danio rerio infected by the microsporidian pathogen pseudoloma neurophilia. | abstract zebrafish are a powerful model organism to study disease. like other animal models, danio rerio colonies are at risk of pathogenic infection. microsporidia, a group of intracellular fungus-like parasites, are one potential threat. microsporidian spores germinate and spread causing pathological changes in the central nervous system, skeletal muscle, and other anatomic sites. infection can impair breeding, cause other morbidities, and ultimately be lethal. previously, detecting microspori ... | 2014 | 24707848 |