| deciphering the biology of deltasatellites from the new world: maintenance by new world begomoviruses and whitefly transmission. | deltasatellites are small noncoding dna satellites associated with begomoviruses. the study presented here has investigated the biology of two deltasatellites found in wild malvaceous plants in the new world (nw). infectious clones of two nw deltasatellites (from malvastrum coromandelianum and sidastrum micranthum) and associated begomoviruses were constructed. infectivity in nicotiana benthamiana and their natural malvaceous hosts was assessed. the nw deltasatellites were not able to spread aut ... | 2016 | 27400152 |
| characterization and distribution of tomato yellow margin leaf curl virus, a begomovirus from venezuela. | a begomovirus causing mottling and leaf deformation in tomato from the state of mérida was cloned and sequenced. the virus has a bipartite genome comprised of a dna-a (2,572 nucleotides) and a dna-b (2,543 nucleotides) with a genome organization typical of new world begomoviruses. both components share a common region of 115 nucleotides with 98 % sequence identity. phylogenetic analysis indicated that while no virus sequences were closely related, the a component was distantly related to those o ... | 2013 | 23064695 |
| begomovirus diversity in tomato crops and weeds in ecuador and the detection of a recombinant isolate of rhynchosia golden mosaic yucatan virus infecting tomato. | viral diseases caused by begomoviruses are of economic importance due to their adverse effects on the production of tropical and subtropical crops. in ecuador, despite reports of significant infestations of bemisia tabaci in the late 1990s, only very recently has a begomovirus, tomato leaf deformation virus (toldev, also present in peru), been reported in tomato. toldev is the first monopartite begomovirus discovered that originated in the americas, and its presence in ecuador highlights the nee ... | 2014 | 24623091 |
| characterization of a new world monopartite begomovirus causing leaf curl disease of tomato in ecuador and peru reveals a new direction in geminivirus evolution. | all characterized whitefly-transmitted geminiviruses (begomoviruses) with origins in the new world (nw) have bipartite genomes composed of a dna-a and dna-b component. recently, an nw begomovirus lacking a dna-b component was associated with tomato leaf curl disease (tolcd) in peru, and it was named tomato leaf deformation virus (toldev). here, we show that isolates of toldev associated with tolcd in ecuador and peru have a single, genetically diverse genomic dna that is most closely related to ... | 2013 | 23468482 |
| fulfilling koch's postulates confirms the monopartite nature of tomato leaf deformation virus: a begomovirus native to the new world. | the monopartite nature of the begomovirus tomato leaf deformation virus (toldev) reported in peru is demonstrated here. the dna molecule cloned from an infected plant was shown to be fully infectious in tomatoes inducing leaf curling and stunted growth similar to that observed in field-infected plants. the viral dna was reisolated from systemically infected tissues of inoculated plants, thus fulfilling koch's postulates. toldev was demonstrated, therefore, as the causal agent of the disease synd ... | 2013 | 23415858 |