| differential expression of two isolates of beak and feather disease virus capsid protein in escherichia coli. | expression of recombinant beak and feather disease virus (bfdv) capsid-associated protein (cap) has relied on inefficient techniques that typically produce low yields or use specialized expression systems, which greatly increase the cost and expertise required for mass production. an escherichia coli system was used to express recombinant bfdv cap derived from two isolates of bfdv, from a long-billed corella (cacatua tenuirostris) and an orange-bellied parrot (obp; neophema chrysogaster). purifi ... | 2013 | 23403150 |
| a survey of a mixed species aviary provides new insights into the pathogenicity, diversity, evolution, host range, and distribution of psittacine and passerine adenoviruses. | a bourke's parrot (neopsephotus bourkii) originating from an aviary in australia, containing two species of parrots, five species of finch and a species of dove, was presented for necropsy. the bourke's parrot died from gastritis caused by macrorhabdus ornithogaster, but also had an interstitial nephritis and ureteritis with adenovirus-like inclusion bodies within collecting duct epithelial cells. the adenovirus causing the lesions was shown to be psittacine adenovirus-2 (psadv-2) using a pcr as ... | 2019 | 31081348 |
| mutability dynamics of an emergent single stranded dna virus in a naïve host. | quasispecies variants and recombination were studied longitudinally in an emergent outbreak of beak and feather disease virus (bfdv) infection in the orange-bellied parrot (neophema chrysogaster). detailed health monitoring and the small population size (<300 individuals) of this critically endangered bird provided an opportunity to longitudinally track viral replication and mutation events occurring in a circular, single-stranded dna virus over a period of four years within a novel bottleneck p ... | 2014 | 24416396 |
| evidence of psittacine beak and feather disease virus spillover into wild critically endangered orange-bellied parrots (neophema chrysogaster). | we report the recent emergence of a novel beak and feather disease virus (bfdv) genotype in the last remaining wild population of the critically endangered orange-bellied parrot (neophema chrysogaster). this virus poses a significant threat to the recovery of the species and potentially its survival in the wild. we used pcr to detect bfdv in the blood of three psittacine beak and feather disease (pbfd)-affected wild orange-bellied parrot fledglings captured as founders for an existing captive br ... | 2014 | 24484492 |
| repeat spillover of beak and feather disease virus into an endangered parrot highlights the risk associated with endemic pathogen loss in endangered species. | conservation efforts for the orange-bellied parrot (neophema chrysogaster), one of the world's most critically endangered bird species, have been hampered by beak and feather disease virus (bfdv) spillover infection. to understand the vulnerability of orange-bellied parrots to potential reservoirs of infection we investigated geographic versus taxonomic structure in 160 full-genome and 319 partial rep gene bfdv sequences from captive and wild orange-bellied parrots and other wild parrot species ... | 2020 | 33600597 |
| rapid genotyping of beak and feather disease virus using high-resolution dna melt curve analysis. | beak and feather disease virus (bfdv) is a significant pathogen both for wild and captive psittacine birds globally. genotypic differentiation of bfdv isolates is crucial to establish effective control strategies for the conservation of endangered species and epidemiological investigations of disease outbreaks. the technique developed in this study is a simple, rapid and inexpensive genotyping method for bfdv using pcr and subsequent high-resolution melt (hrm) curve analysis. this was achieved u ... | 2014 | 25102431 |
| microsatellite loci and the complete mitochondrial dna sequence characterized through next generation sequencing and de novo genome assembly for the critically endangered orange-bellied parrot, neophema chrysogaster. | a suite of polymorphic microsatellite markers and the complete mitochondrial genome sequence was developed by next generation sequencing (ngs) for the critically endangered orange-bellied parrot, neophema chrysogaster. a total of 14 polymorphic loci were identified and characterized using dna extractions representing 40 individuals from melaleuca, tasmania, sampled in 2002. we observed moderate genetic variation across most loci (mean number of alleles per locus = 2.79; mean expected heterozygos ... | 2013 | 23114913 |
| psittacid adenovirus-2 infection in the critically endangered orange-bellied parrot (neophema chrysogastor): a key threatening process or an example of a host-adapted virus? | psittacid adenovirus-2 (psadv-2) was identified in captive orange-bellied parrots (neophema chrysogastor) during a multifactorial cluster of mortalities at the adelaide zoo, south australia, and an outbreak of pseudomonas aeruginosa septicaemia at the tasmanian department of primary industries, parks, water and environment captive breeding facility, taroona, tasmania. this was the first time that an adenovirus had been identified in orange-bellied parrots and is the first report of psadv-2 in au ... | 2019 | 30811501 |