| use of dried high-phenolic laden host leaves for virus and viroid preservation and detection by pcr methods. | the efficiency of rna extraction for apricot latent virus (aplv), plum bark necrosis stem pitting associated virus (pbnspav), prunus necrotic ring spot virus (pnrsv), potato virus y (pvy), and apple scar skin viroid (assvd) from infected hosts is of great importance for molecular diagnosis by the polymerase chain reaction (pcr). a method is described for drying tissue to overcome phenolic inhibitors of viral rna. this study showed that the infected host leaves, dried at 65 degrees c for 2 days a ... | 2006 | 16879877 |
| molecular characterization and detection of plum bark necrosis stem pitting-associated virus. | the complete rna genome of plum bark necrosis stem pitting-associated virus (pbnspav) was cloned and sequenced and was determined to be 14, 214 nts long. the genome structure revealed seven major open reading frames (orfs), and nontranslated regions at the 5' and 3' ends. pbnspav represents the simplest genome organization in the genus ampelovirus, family closteroviridae. the orfs 1a and 1b encode, respectively, a large polyprotein with a molecular mass (mr) of 259.6 kda containing conserved dom ... | 2007 | 17885796 |
| complete nucleotide sequence and genome organization of pineapple mealybug wilt-associated virus-1. | pineapple mealybug wilt-associated virus-1 (pmwav-1; family closteroviridae, genus ampelovirus) belongs to a complex of mealybug-transmissible viruses found in pineapple worldwide. in this study, the complete genome of pmwav-1 was sequenced and found to be 13.1 kb in length, making it the smallest in the family. the genome encoded seven open reading frames (orfs) and was unusual for an ampelovirus due to the lack of an intergenic region between the rdrp and p6 orfs, an orf encoding a relatively ... | 2008 | 18283409 |
| complete genomic characterization of plum bark necrosis stem pitting-associated virus infecting sweet cherry in china. | plum bark necrosis stem pitting-associated virus (pbnspav) causes the plum bark necrosis stem pitting-associated disease. we obtained the complete genome of a pbnspav isolate (pbnspav-ta) using small rna deep sequencing followed by overlapping rt-pcr. to our knowledge, this is the first report of a completed genome of pbnspav identified from cherry trees. | 2016 | 27198034 |
| genetic diversity and molecular evolution of plum bark necrosis stem pitting-associated virus from china. | plum bark necrosis stem pitting-associated virus (pbnspav), a member of the genus ampelovirus in the family closteroviridae, infects different prunus species and has a worldwide distribution. yet the population structure and genetic diversity of the virus is still unclear. in this study, sequence analyses of a partial heat shock protein 70 homolog (hsp70h) gene and coat protein (cp) gene of pbnspav isolates from seven prunus species grown in china revealed a highly divergent chinese pbnspav popu ... | 2014 | 25144238 |
| characterization by deep sequencing of divergent plum bark necrosis stem pitting-associated virus (pbnspav) isolates and development of a broad-spectrum pbnspav detection assay. | plum bark necrosis stem pitting-associated virus (pbnspav), the causal agent of plum bark necrosis stem pitting disease, belongs to the genus ampelovirus in the family closteroviridae. the complete genome sequence of pbnspav isolates from four prunus sources was determined by pyrosequencing. all isolates showed the same genomic organization as the pbnspav reference isolate. the least divergent isolate, found in a peach tree from china, showed an overall 91.8% of nucleotide identity with the type ... | 2014 | 24328491 |