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isolation of bacteria from mouse caecal samples and description of bacteroides sartorii sp. nov.caecal samples from wild-type and tnf(deltaare) mice were cultured on selective media containing bile salts, amino acids or casein macro-peptides. twenty-two strains were isolated and identified by 16s rrna gene sequencing. twenty-one strains showed >98% similarity to known bacteria (blautia spp., clostridium innocuum, enterococcus spp., escherichia coli, lactobacillus murinus, parabacteroides goldsteinii and shigella dysenteriae). one additional isolate, strain a-c2-0, was a new bacterium. the ...201020369227
bacteroides sartorii is an earlier heterotypic synonym of bacteroides chinchillae and has priority.the recently proposed species bacteroides chinchillae shared more than 99.4 % 16s rrna gene sequence similarity with bacteroides sartorii although these two species appear to be not similar from their published descriptions. the aim of this study was to perform phenotypic and genetic analyses of both species to clarify their taxonomic position. b. chinchillae jcm 16497(t) exhibited high hsp60 gene sequence similarity with b. sartorii jcm 17136(t) (100 %) as well as b. chinchillae jcm 16498 (100 ...201121764984
discordance between changes in the gut microbiota and pathogenicity in a mouse model of spontaneous colitis.under conventional conditions, mice deficient in core 1-derived o-glycans (tm-iec c1galt1(-/-)), which have a defective mucus layer, experienced spontaneous inflammation of the colon. analysis of fecal bacterial populations by pyrosequencing of 16s rrna gene showed that disease in conventional tm-iec c1galt1(-/-) was associated with shifts in the microbiota manifested by increases in lactobacillus and clostridium species, and decreases in unclassified ruminococcaceae and lachnospiraceae. under g ...201624662098
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