| prevalence, molecular characterization and zoonotic potential of cryptosporidium spp. in goats in henan and chongqing, china. | to estimate the prevalence and public health significance of cryptosporidiosis in goats in china, 1265 fecal samples from seven farms in henan province and chongqing city were examined for cryptosporidium oocysts. the overall infection rate of cryptosporidium spp. was 3.48% (44/1256). significant difference was observed among age groups, with the post weaned kids having the highest infection rate (4.58%; ρ<0.01). cryptosporidium spp. were characterized by pcr-restriction fragment length polymorp ... | 2014 | 24721256 |
| cryptosporidium xiaoi n. sp. (apicomplexa: cryptosporidiidae) in sheep (ovis aries). | a new species, cryptosporidium xiaoi, is described from sheep. oocysts of c. xiaoi, previously identified as the cryptosporidium bovis-like genotype or as c. bovis from sheep in spain, tunisia, united kingdom, and the united states are recorded as such in genbank (eu408314-eu408317, eu327318-eu327320, ef362478, ef514234, dq991389, and ef158461). oocysts obtained from naturally infected sheep were infectious for a lamb and oocysts from that lamb were infectious for three other lambs. the prepaten ... | 2009 | 19501967 |
| sentinels in a climatic outpost: endoparasites in the introduced muskox (ovibos moschatus wardi) population of dovrefjell, norway. | we assessed the occurrence of endoparasite eggs, cysts, oocysts and larvae in the muskox population of dovrefjell, norway, during june and august 2012. this population originates from 13 calves translocated from eastern greenland during the 1950s. a total of 167 faecal samples were collected, of which 49% came from identified individuals: 165 were examined by the baermann and 95 by mcmaster techniques and 167 by immunofluorescence antibody test (ifat). lungworm larvae recovered in the baermanns ... | 2014 | 25161914 |
| prevalence and molecular characterization of cryptosporidium in goats across four provincial level areas in china. | this study assessed the prevalence, species and subtypes of cryptosporidium in goats from guangdong province, hubei province, shandong province, and shanghai city of china. six hundred and four fecal samples were collected from twelve goat farms, and the overall infection rate was 11.4% (69/604). goats infected with cryptosporidium were found in eleven farms across four provincial areas, and the infection rate ranged from 2.9% (1/35) to 25.0% (9/36). three cryptosporidium species were identified ... | 2014 | 25343501 |
| periparturient transmission of cryptosporidium xiaoi from ewes to lambs. | the mechanism for the maintenance of cryptosporidium infection in sheep between yearly lambing periods is not clear. previously, periparturient shedding of oocysts as the result of stress from lambing was suspected to be a mechanism for the initiation of cryptosporidium infection in lambs, but this has never been verified by genotyping studies. in this study, fecal specimens from four age groups of sheep in inner mongolia, china were examined for cryptosporidium spp. by pcr-restricted fragment l ... | 2013 | 23953146 |
| molecular characterization of cryptosporidium spp. in pre-weaned kids in a dairy goat farm in western france. | a longitudinal study was undertaken to characterize the course of cryptosporidium infection in a dairy goat farm located in western france. two cohorts of twenty-five and fifteen animals, respectively, were sampled once a week from birth to weaning. each individual fecal sample was screened using direct immunofluorescence (ift) and if found positive, the cryptosporidium species was identified using pcr analysis. seventeen (68% [95% ci: 44-91]) animals were positive at least once during the first ... | 2013 | 23218587 |
| cryptosporidium species and subtype analysis in diarrhoeic pre-weaned lambs and goat kids from north-western spain. | faecal specimens from diarrhoeic pre-weaned lambs (n = 171) and goat kids (n = 118) were collected in 37 sheep and 23 goat flocks, respectively, from nw spain and microscopically examined for the presence of cryptosporidium oocysts. positive specimens were selected for molecular characterization. presence of cryptosporidium oocysts were significantly higher in specimens from goat kids (62.7%) than from lambs (31.6%). pcr products of the ssu rrna locus were obtained for 108 isolates, and three cr ... | 2015 | 26212102 |
| infection rate of giardia duodenalis, cryptosporidium spp. and enterocytozoon bieneusi in cashmere, dairy and meat goats in china. | cryptosporidiosis, microsporidiosis, and giardiasis contribute significantly to the high burden of zoonotic diarrhea worldwide. goats constitute an important species in animal agriculture by providing cashmere wool, meat, and dairy products for human consumption. however, zoonotic pathogens with the potential to cause morbidity and to degrade production have been reported frequently in goats recently. the present study examined 629 fecal specimens from goats, including 315 cashmere goats, 170 da ... | 2016 | 27017915 |
| novel quantitative taqman real-time pcr assays for detection of cryptosporidium at the genus level and genotyping of major human and cattle-infecting species. | development of taqman mgb real-time pcr assays for quantitative typing of major cattle and human-pathogenic cryptosporidium species. | 2013 | 23230846 |
| distribution of cryptosporidium species in tibetan sheep and yaks in qinghai, china. | few data are available on the distribution of cryptosporidium species in tibetan sheep and yaks, which are free-range animals living in a cold, low oxygen, and high ultraviolet radiation habitat. in this study, 904 fecal specimens were collected from 350 tibetan sheep and 554 yaks in six counties. cryptosporidium spp. were detected and differentiated by pcr and sequence analyses. altogether, 43 (12.3%) tibetan sheep and 158 (28.5%) yaks were positive for cryptosporidium spp. in tibetan sheep, cr ... | 2016 | 26790738 |
| microscopic and molecular detection of cryptosporidium andersoni and cryptosporidium xiaoi in wastewater samples of tehran province, iran. | as a waterborne pathogen, cryptosporidium is one of the most common causes of gastroenteritis in human and hoofed livestock animals. this study aimed to investigate the distribution of cryptosporidium spp. in human and livestock wastewaters in iran, by the 18s rrna sequence analysis. | 2017 | 28127361 |
| longitudinal investigation of protozoan parasites in meat lamb farms in southern western australia. | in this study, 96 faecal samples were collected from pregnant merino ewes, at two broad-acre, commercial sheep farms in southern western australia, on two separate occasions (16 and 2 weeks prior to lambing). following lambing, 111 (farm a) and 124 (farm b) female crossbred lambs (2-6 weeks old), were individually identified using ear tags (a numbered tag and a radio-frequency tag). a total of 1155 faecal samples were collected only from these individually identified lambs on five separate sampl ... | 2011 | 21733584 |
| identification of novel cryptosporidium genotypes in kangaroos from western australia. | a total of 763 faecal samples were collected from western grey kangaroos (macropus fuliginosus) in western australia and screened for the presence of cryptosporidium by pcr at the 18s ribosomal rna (rrna) locus. samples that were positive at the 18s locus were also amplified at the actin locus. the overall prevalence was 9.3% (71/763). at the 18s rrna locus, sequences were obtained for 28 of the 71 positives. sequence analysis identified four species; cryptosporidium fayeri in seven isolates, cr ... | 2011 | 21402448 |
| identification of cryptosporidium xiaoi in diarrhoeic goat kids (capra hircus) in spain. | faecal specimens from five diarrhoeic goat kids (capra hircus) younger than 21 days were collected in a goat farm in galicia (nw spain) and examined for the presence of cryptosporidium oocysts. two cryptosporidium-positive isolates were detected and selected for molecular examination. a banding pattern indicative of cryptosporidium bovis was obtained after restriction analyses of pcr products from small-subunit rrna genes. however, both positive isolates were identified as cryptosporidium xiaoi ... | 2010 | 20537797 |
| cervine genotype is the major cryptosporidium genotype in sheep in china. | to identify cryptosporidium species/genotypes in sheep in china and to elucidate the endemic transmission of cryptosporidiosis, a total of 1,701 fecal samples from five farms in four prefectures in henan province (central china) were examined. eighty-two cryptosporidium-positive samples were analyzed by polymerase chain reaction (pcr)-restriction fragment length polymorphism analysis of the small subunit (ssu) rrna gene and pcr analysis of the 60 kda glycoprotein (gp60) gene, and 41 were further ... | 2010 | 19904561 |