| a genome-wide survey for host response of silkworm, bombyx mori during pathogen bacillus bombyseptieus infection. | host-pathogen interactions are complex relationships, and a central challenge is to reveal the interactions between pathogens and their hosts. bacillus bombysepticus (bb) which can produces spores and parasporal crystals was firstly separated from the corpses of the infected silkworms (bombyx mori). bb naturally infects the silkworm can cause an acute fuliginosa septicaemia and kill the silkworm larvae generally within one day in the hot and humid season. bb pathogen of the silkworm can be used ... | 2009 | 19956592 |
| midgut immune responses induced by bacterial infection in the silkworm, bombyx mori. | insect gut epithelial cells produce reactive oxygen species (ros) and antimicrobial peptides (amps) to protect hosts from pathogenic microorganisms. in this study, we evaluate the pathogenicity of pseudomonas aeruginosa and bacillus bombysepticus in the silkworm, bombyx mori. survival curves show that b. bombysepticus is deadly when larval silkworms are infected orally. bacterial infection caused intestinal hydrogen peroxide (h2o2) and nitric oxide (no) levels to increase significantly by 8 and ... | 2015 | 26465135 |
| genome-wide identification and immune response analysis of serine protease inhibitor genes in the silkworm, bombyx mori. | in most insect species, a variety of serine protease inhibitors (spis) have been found in multiple tissues, including integument, gonad, salivary gland, and hemolymph, and are required for preventing unwanted proteolysis. these spis belong to different families and have distinct inhibitory mechanisms. herein, we predicted and characterized potential spi genes based on the genome sequences of silkworm, bombyx mori. as a result, a total of eighty spi genes were identified in b. mori. these spi gen ... | 2012 | 22348050 |
| structural, evolutionary and functional analysis of apn genes in the lepidoptera bombyx mori. | aminopeptidases n (apns), the receptors of bacillus thuringiensis (bt) toxin in the lepidopteran midgut, are involved in the bt pathogen infection mechanism. in the present work, we screened 102 apns from silkdb, butterflybase and monarchbase; 16 apns were identified from the silkworm (bombyx mori) and 24 from the monarch butterfly (danaus plexippus). syntenic and phylogenetic tree analysis showed that apn genes have developed multi-family genes before evolutionary divergence of the lepidoptera. ... | 2014 | 24286860 |
| the initial analysis of a serine proteinase gene (accsp10) from apis cerana cerana: possible involvement in pupal development, innate immunity and abiotic stress responses. | serine proteinases play important roles in innate immunity and insect development. we isolated a serine proteinase gene, designated accsp10, from the chinese honeybees (apis cerana cerana). rt-qpcr and a western blot analysis at different pupal development stages indicated that accsp10 might be involved in melanin formation in pupae and promote pupal development. in adult workers, the expression of accsp10 was upregulated by treatments mimicking harmful environments such as the presence of bacil ... | 2017 | 28695333 |
| complete genome sequence of bacillus bombysepticus, a pathogen leading to bombyx mori black chest septicemia. | bacillus bombysepticus is a gram-positive spore-forming bacterium. here, we announce the first complete genome sequence of this organism isolated from the cadavers of silkworm larvae that had been sick. the genome contains a single circular chromosome and a circular plasmid. analyses of the b. bombysepticus genome will provide insights into its pathomechanisms and biology. | 2014 | 24831136 |
| pc, a novel oral insecticidal toxin from bacillus bombysepticus involved in host lethality via apn and btr-175. | insect pests have developed resistance to chemical insecticides, insecticidal toxins as bioinsecticides or genetic protection built into crops. consequently, novel, orally active insecticidal toxins would be valuable biological alternatives for pest control. here, we identified a novel insecticidal toxin, parasporal crystal toxin (pc), from bacillus bombysepticus (bb). pc shows oral pathogenic activity and lethality towards silkworms and cry1ac-resistant helicoverpa armigera strains. in vitro as ... | 2015 | 26057951 |
| identification of mbf2 family genes in bombyx mori and their expression in different tissues and stages and in response to bacillus bombysepticus infection and starvation. | the multiprotein bridge factor 2 (mbf2) gene was first identified as a co-activator involved in bmftz-f1-mediated activation of the fushi tarazu gene. herein, nine homologous genes of mbf2 gene are identified. evolutionary analysis showed that this gene family is insect-specific and that the family members are closely related to response to pathogens (repat) genes. tissue distribution analysis revealed that these genes could be expressed in a tissue-specific manner. developmental profiles analys ... | 2016 | 27121992 |
| bacillus bombysepticus α-toxin binding to g protein-coupled receptor kinase 2 regulates camp/pka signaling pathway to induce host death. | bacterial pathogens and their toxins target host receptors, leading to aberrant behavior or host death by changing signaling events through subversion of host intracellular camp level. this is an efficient and widespread mechanism of microbial pathogenesis. previous studies describe toxins that increase camp in host cells, resulting in death through g protein-coupled receptor (gpcr) signaling pathways by influencing adenylyl cyclase or g protein activity. g protein-coupled receptor kinase 2 (grk ... | 2016 | 27022742 |
| genome-wide identification and expression analysis of serine proteases and homologs in the silkworm bombyx mori. | serine proteases (sps) and serine proteases homologs (sphs) are a large group of proteolytic enzymes, with important roles in a variety of physiological processes, such as cell signalling, defense and development. genome-wide identification and expression analysis of serine proteases and their homologs in the silkworm might provide valuable information about their biological functions. | 2010 | 20576138 |