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molecular characterization of a closterovirus from carrot and its identification as a german isolate of carrot yellow leaf virus.a high-molecular-weight dsrna (approximately 15 kbp) was isolated from chlorotic leaves of a carrot plant and used for determining the entire nucleotide sequence of a closterovirus. the complete genome of this carrot closterovirus (ccv) was 16.4 kb in length and contained ten open reading frames (orfs). the genome organization of ccv resembled that of beet yellow stunt virus, but orf2 and orf3 were in a reversed order. based on hsp70h sequences, ccv is most closely related to carnation necrotic ...200919575278
carrot yellow leaf virus is associated with carrot internal necrosis.internal necrosis of carrot has been observed in uk carrots for at least 10 years, and has been anecdotally linked to virus infection. in the 2009 growing season some growers had up to 10% of yield with these symptoms. traditional diagnostic methods are targeted towards specific pathogens. by using a metagenomic approach with high throughput sequencing technology, other, as yet unidentified causes of root necrosis were investigated. additionally a statistical analysis has shown which viruses are ...201425365290
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