development and characterization of new cell line bph22 from midgut epithelial cells of poekilocerus pictus (fabricius, 1775).poekilocerus pictus is a painted grasshopper, which feeds on calotropis sp. containing the cardiac glycosides. a new cell line bph22 is developed from midgut of p. pictus to study its unique physiology and biochemistry. initially, the graces insect medium is used with 10% (v/v) fetal bovine serum. after four passages, the serum quantity was reduced up to 0%.the lag phase in growth curve was 5 d and log phase is up to 10-11 d. the addition of calotropis extract in graces medium enhanced the growt ...201020927604
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