| enterocytozoon hepatopenaei sp. nov. (microsporida: enterocytozoonidae), a parasite of the black tiger shrimp penaeus monodon (decapoda: penaeidae): fine structure and phylogenetic relationships. | a new microsporidian species, enterocytozoon hepatopenaei sp. nov., is described from the hepatopancreas of the black tiger shrimp penaeus monodon (crustacea: decapoda). different stages of the parasite are described, from early sporogonal plasmodia to mature spores in the cytoplasm of host-cells. the multinucleate sporogonal plasmodia existed in direct contact with the host-cell cytoplasm and contained numerous small blebs at the surface. binary fission of the plasmodial nuclei occurred during ... | 2009 | 19527727 |
| laboratory cohabitation challenge model for shrimp hepatopancreatic microsporidiosis (hpm) caused by enterocytozoon hepatopenaei (ehp). | enterocytozoon hepatopenaei (ehp) causes hepatopancreatic microsporidiosis (hpm) in shrimp. it is probably endemic in australasia and was first characterized and named from the giant or black tiger shrimp penaeus monodon from thailand in 2009. later, it was also found to infect exotic penaeus vannamei imported for cultivation in asia. hpm is not normally associated with shrimp mortality, but information from shrimp farmers indicates that it is associated with significant growth retardation that ... | 2017 | 28056950 |
| loop-mediated isothermal amplification combined with colorimetric nanogold for detection of the microsporidian enterocytozoon hepatopenaei in penaeid shrimp. | enterocytozoon hepatopenaei is an emerging microsporidian parasite that has been linked to recent losses caused by white faeces syndrome (wfs) in cultivated giant or black tiger shrimp penaeus (penaeus) monodon and whiteleg shrimp penaeus (litopenaeus) vannamei in asia. to more accurately assess its impact on shrimp production and to determine reservoir carriers for control measures, our objective was to establish a loop-mediated isothermal amplification (lamp) assay combined with colorimetric n ... | 2013 | 23387348 |
| the microsporidian enterocytozoon hepatopenaei is not the cause of white feces syndrome in whiteleg shrimp penaeus (litopenaeus) vannamei. | the microsporidian enterocytozoon hepatopenaei was first described from thailand in 2009 in farmed, indigenous giant tiger shrimp penaeus (penaeus) monodon. the natural reservoir for the parasite is still unknown. more recently, a microsporidian closely resembling it in morphology and tissue preference was found in thai-farmed, exotic, whiteleg shrimp penaeus (litopenaeus) vannamei exhibiting white feces syndrome (wfs). our objective was to compare the newly found pathogen with e. hepatopenaei a ... | 2013 | 23856195 |
| biochemical changes and tissue distribution of enterocytozoon hepatopenaei (ehp) in naturally and experimentally ehp-infected whiteleg shrimp, litopenaeus vannamei (boone, 1931), in india. | stunted growth in pond-reared litopenaeus vannamei was observed in different farms located in tamil nadu and andhra pradesh, india. no mortality was associated with stunted growth. pcr assay on these samples revealed the presence of enterocytozoon hepatopenaei (ehp) in stunted shrimp. tissue distribution of ehp in naturally and experimentally infected shrimp was studied by pcr and histology. histological examination revealed the presence of ehp in hepatopancreas and gut, but not in other organs. ... | 2017 | 27524805 |
| dense populations of the microsporidian enterocytozoon hepatopenaei (ehp) in feces of penaeus vannamei exhibiting white feces syndrome and pathways of their transmission to healthy shrimp. | white feces syndrome (wfs) is an emerging problem for penaeid shrimp farming industries in se asia countries, thailand, malaysia, vietnam, indonesia, china, and in india. this occurrence of this syndrome is usually first evidenced by the appearance of white fecal strings floating on surface of the shrimp ponds. the gross signs of affected shrimp include the appearance of a whitish hindgut and loose carapace, and it is associated with reduced feeding and growth retardation. to investigate the nat ... | 2016 | 27530403 |
| a nested pcr assay to avoid false positive detection of the microsporidian enterocytozoon hepatopenaei (ehp) in environmental samples in shrimp farms. | hepatopancreatic microsporidiosis (hpm) caused by enterocytozoon hepatopenaei (ehp) is an important disease of cultivated shrimp. heavy infections may lead to retarded growth and unprofitable harvests. existing pcr detection methods target the ehp small subunit ribosomal rna (ssu rrna) gene (ssu-pcr). however, we discovered that they can give false positive test results due to cross reactivity of the ssu-pcr primers with dna from closely related microsporidia that infect other aquatic organisms. ... | 2016 | 27832178 |
| decay of the glycolytic pathway and adaptation to intranuclear parasitism within enterocytozoonidae microsporidia. | glycolysis and oxidative phosphorylation are the fundamental pathways of atp generation in eukaryotes. yet in microsporidia, endoparasitic fungi living at the limits of cellular streamlining, oxidative phosphorylation has been lost: energy is obtained directly from the host or, during the dispersive spore stage, via glycolysis. it was therefore surprising when the first sequenced genome from the enterocytozoonidae - a major family of human and animal-infecting microsporidians - appeared to have ... | 2017 | 28345194 |
| high prevalence of enterocytozoon hepatopenaei in shrimps penaeus monodon and litopenaeus vannamei sampled from slow growth ponds in india. | hepatopancreatic microsporidiosis in cultivated litopenaeus vannamei and penaeus monodon is caused by the newly emerged pathogen enterocytozoon hepatopenaei (ehp). it has been detected in shrimp cultured in china, vietnam and thailand and is suspected to have occurred in malaysia and indonesia and to be associated with severely retarded growth. due to retarded shrimp growth being reported at farms in the major grow-out states of tamilnadu, andhra pradesh and odisha in india, shrimp were sampled ... | 2016 | 27503918 |
| development of in situ hybridization and pcr assays for the detection of enterocytozoon hepatopenaei (ehp), a microsporidian parasite infecting penaeid shrimp. | a microsporidian parasite, enterocytozoon hepatopenaei (abbreviated as ehp), is an emerging pathogen for penaeid shrimp. ehp has been found in several shrimp farming countries in asia including vietnam, thailand, malaysia, indonesia and china, and is reported to be associated with growth retardation in farmed shrimp. we examined the histological features from infected shrimp collected from vietnam and brunei, these include the presence of basophilic inclusions in the hepatopancreas tubule epithe ... | 2015 | 26146228 |
| detection of a new microsporidium perezia sp. in shrimps penaeus monodon and p. indicus by histopathology, in situ hybridization and pcr. | samples of microsporidia-infected shrimps exhibiting clinical signs of cotton shrimp disease were collected from madagascar, mozambique, and the kingdom of saudi arabia from 2005 to 2014. the tails of the infected shrimps appeared opaque and whitish; subsequent histological examination revealed the presence of cytoplasmic inclusions and mature spores in tissues of the muscle, hepatopancreas, gills, heart, and lymphoid organ. pcr analysis targeting the small subunit rdna (ssu rdna) from infected ... | 2016 | 27409240 |