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practical pcr tools for the delineation of contracaecum rudolphii a and contracaecum rudolphii b (ascaridoidea: anisakidae) using genetic markers in nuclear ribosomal dna.using genetic markers defined previously in the internal transcribed spacers (its-1 and its-2) of nuclear ribosomal dna (rdna), pcr-coupled restriction fragment length polymorphism (pcr-rflp) and specific pcr assays were established for the specific detection of each of two morphologically indistinguishable operational taxonomic units (contracaecum rudolphii a and contracaecum rudolphii b) within contracaecum rudolphii (s.l.) and their differentiation from contracaecum septentrionale, a closely ...200717029878
molecular identification of two strains of third-stage larvae of contracaecum rudolphii sensu lato (nematoda: anisakidae) from fish in poland.contracaecum sp. larvae (l3) from fish were identified using nucleotide sequences of the internal transcribed spacers its-1 and its-2 of the ribosomal dna. the nematode larvae originated from fish in a freshwater situation (crucian carp carassius carassius, from selment wielki lake in mazury, northeastern poland) and a brackish-water region (caspian round goby neogobius melanostomus from the baltic sea, gdafisk bay at the polish coast). two strains (contracaecum rudolphii a and b) of contracaecu ...200717918390
molecular evidence for the occurrence of contracaecum rudolphii a (nematoda: anisakidae) in shag phalacrocorax aristotelis (linnaeus) (aves: phalacrocoracidae) from sardinia (western mediterranean sea).specimens of contracaecum rudolphii hartwich, 1964 (nematoda: anisakidae) from phalacrocorax aristotelis (linnaeus) from the archipelago of la maddalena (sardinia, western mediterranean sea) were characterised genetically and compared with c. rudolphii a sensu d'amelio et al. 1990 and c. rudolphii b sensu d'amelio et al. 1990 from phalacrocorax carbo sinensis (blumenbach) from north-eastern italy, and with c. rudolphii c sensu d'amelio et al. 2007 from phalacrocorax auritus (lesson) from west-ce ...200818571974
genetic and morphological evidences for the existence of a new species of contracaecum (nematoda: anisakidae) parasite of phalacrocorax brasilianus (gmelin) from chile and its genetic relationships with congeners from fish-eating birds.abstract contracaecum australe n. sp. is described from the neotropic cormorant phalacrocorax brasilianus in chile based on morphology and the sequence analyses of multiple loci, i.e., mitochondrial cytochrome oxidase 2, mtdna cox-2 , the small subunit of the mitochondrial ribosomal rna gene, rrns , and the its-1 and its-2 regions of nuclear ribosomal dna. moreover, sequence analysis of the same genes was carried out on the morphospecies contracaecum chubutensis garbin et al. (2008) from phalacr ...201121506861
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