distribution and molecular diversity of three cucurbit-infecting poleroviruses in china. | cucurbit aphid-borne yellows virus (cabyv) and melon aphid-borne yellows virus (mabyv) have been found to be associated with cucurbit yellowing disease in china. our report identifies for the first time a third distinct polerovirus, tentatively named suakwa aphid-borne yellows virus (sabyv), infecting suakwa vegetable sponge. to better understand the distribution and molecular diversity of these three poleroviruses infecting cucurbits, a total of 214 cucurbitaceous crop samples were collected fr ... | 2009 | 19664664 |
incidence and molecular diversity of poleroviruses infecting cucurbit crops and weed plants in thailand. | overall, 244 samples of cucurbit crops with yellowing symptoms and selected weed species, from 15 provinces in thailand, were screened by rt-pcr using primers polero-cp-f and polero-cp-r. a total of 160 samples (~66%) were infected by poleroviruses. analysis of a 1.4 kb region covering the 3' rna-dependent rna polymerase (rdrp) gene, the intergenic non-coding region (incr), and the coat protein (cp), showed that four poleroviruses, namely, cucurbit aphid-borne yellows virus (cabyv), luffa aphid- ... | 2017 | 28352973 |
pepo aphid-borne yellows virus: a new species in the genus polerovirus. | pepo aphid-borne yellows virus (pabyv) has been proposed as a putative representative of a new species in the genus polerovirus in the family luteoviridae. the genomes of two south african (sa) isolates of cucurbit-infecting pabyv were described in this record. total rna, extracted from a pattypan (cucurbita pepo l.) and a baby marrow (c. pepo l.) leaf samples, was subjected to next-generation sequencing (ngs) on the hiseq illumina platform. sanger sequencing was subsequently used to authenticat ... | 2017 | 27623838 |
first complete genome sequence of suakwa aphid-borne yellows virus from east timor. | we present here the first complete genomic rna sequence of the polerovirus suakwa aphid-borne yellows virus (sabyv), from east timor. the isolate sequenced came from a virus-infected pumpkin plant. the east timorese genome had a nucleotide identity of 86.5% with the only other sabyv genome available, which is from taiwan. | 2016 | 27469955 |
molecular diversity of poleroviruses infecting cucurbit crops in four countries reveals the presence of members of six distinct species. | when 66 cucurbit samples with yellowing symptoms from fields in mali, the philippines, thailand and uzbekistan were screened by rt-pcr using universal polerovirus primers, 21 were identified as harboring polerovirus rna. when these 21 samples were screened with specific primers for the known cucurbit-infecting poleroviruses, suakwa aphid-borne yellows virus and a recombinant strain of cucurbit aphid-borne yellows virus were detected for the first time in the philippines and thailand. however, se ... | 2014 | 24337720 |