Publications

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distribution and molecular diversity of three cucurbit-infecting poleroviruses in china.cucurbit aphid-borne yellows virus (cabyv) and melon aphid-borne yellows virus (mabyv) have been found to be associated with cucurbit yellowing disease in china. our report identifies for the first time a third distinct polerovirus, tentatively named suakwa aphid-borne yellows virus (sabyv), infecting suakwa vegetable sponge. to better understand the distribution and molecular diversity of these three poleroviruses infecting cucurbits, a total of 214 cucurbitaceous crop samples were collected fr ...200919664664
incidence and molecular diversity of poleroviruses infecting cucurbit crops and weed plants in thailand.overall, 244 samples of cucurbit crops with yellowing symptoms and selected weed species, from 15 provinces in thailand, were screened by rt-pcr using primers polero-cp-f and polero-cp-r. a total of 160 samples (~66%) were infected by poleroviruses. analysis of a 1.4 kb region covering the 3' rna-dependent rna polymerase (rdrp) gene, the intergenic non-coding region (incr), and the coat protein (cp), showed that four poleroviruses, namely, cucurbit aphid-borne yellows virus (cabyv), luffa aphid- ...201728352973
pepo aphid-borne yellows virus: a new species in the genus polerovirus.pepo aphid-borne yellows virus (pabyv) has been proposed as a putative representative of a new species in the genus polerovirus in the family luteoviridae. the genomes of two south african (sa) isolates of cucurbit-infecting pabyv were described in this record. total rna, extracted from a pattypan (cucurbita pepo l.) and a baby marrow (c. pepo l.) leaf samples, was subjected to next-generation sequencing (ngs) on the hiseq illumina platform. sanger sequencing was subsequently used to authenticat ...201727623838
first complete genome sequence of suakwa aphid-borne yellows virus from east timor.we present here the first complete genomic rna sequence of the polerovirus suakwa aphid-borne yellows virus (sabyv), from east timor. the isolate sequenced came from a virus-infected pumpkin plant. the east timorese genome had a nucleotide identity of 86.5% with the only other sabyv genome available, which is from taiwan.201627469955
molecular diversity of poleroviruses infecting cucurbit crops in four countries reveals the presence of members of six distinct species.when 66 cucurbit samples with yellowing symptoms from fields in mali, the philippines, thailand and uzbekistan were screened by rt-pcr using universal polerovirus primers, 21 were identified as harboring polerovirus rna. when these 21 samples were screened with specific primers for the known cucurbit-infecting poleroviruses, suakwa aphid-borne yellows virus and a recombinant strain of cucurbit aphid-borne yellows virus were detected for the first time in the philippines and thailand. however, se ...201424337720
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