Publications

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identification of a novel astrovirus (astrovirus va1) associated with an outbreak of acute gastroenteritis.the etiology of a large proportion of gastrointestinal illness is unknown. in this study, random sanger sequencing and pyrosequencing approaches were used to analyze fecal specimens from a gastroenteritis outbreak of unknown etiology in a child care center. multiple sequences with limited identity to known astroviruses were identified. assembly of the sequences and subsequent reverse transcription-pcr (rt-pcr) and rapid amplification of cdna ends generated a complete genome of 6,586 nucleotides. ...200919706703
human stool contains a previously unrecognized diversity of novel astroviruses.human astroviruses are a leading cause of gastrointestinal disease. since their discovery in 1975, 8 closely related serotypes have been described in humans, and more recently, two new astrovirus species, astrovirus mlb1 and astrovirus va1, were identified in diarrhea patients. in this study, we used consensus astrovirus primers targeting the rna polymerase to define the diversity of astroviruses present in pediatric patients with diarrhea on two continents. from 416 stool specimens comprising t ...200919814825
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