| automated identification of medically important bacteria by 16s rrna gene sequencing using a novel comprehensive database, 16spathdb. | despite the increasing use of 16s rrna gene sequencing, interpretation of 16s rrna gene sequence results is one of the most difficult problems faced by clinical microbiologists and technicians. to overcome the problems we encountered in the existing databases during 16s rrna gene sequence interpretation, we built a comprehensive database, 16spathdb (http://147.8.74.24/16spathdb) based on the 16s rrna gene sequences of all medically important bacteria listed in the manual of clinical microbiology ... | 2011 | 21389154 |
| phylogenetic perspectives of nitrogen-fixing actinobacteria. | it was assumed for a long time that the ability to catalyze atmospheric nitrogen (diazotrophy) has a narrow distribution among actinobacteria being limited to the genus frankia. recently, the number of nitrogen fixation (nifh) genes identified in other non-frankia actinobacteria has dramatically increased and has opened investigation on the origin and emergence of diazotrophy among actinobacteria. during the last decade, mycobacterium flavum, corynebacterium autotrophicum and a fluorescent arthr ... | 2011 | 21779790 |
| first report of gordonibacter pamelaeae bacteremia. | we report the first case of gordonibacter pamelaeae bacteremia, identified by phenotypic tests and 16s rrna sequencing in a patient with disseminated rectosigmoid carcinoma and responsive to amoxicillin-clavulanate. the bacterium was a nonsporulating, anaerobic, gram-positive, nonmotile, coccobacillus that was catalase, arginine dihydrolase, and arginine acrylamidase positive. the gastrointestinal tract is probably its reservoir. | 2010 | 19923481 |
| gordonibacter pamelaeae gen. nov., sp. nov., a new member of the coriobacteriaceae isolated from a patient with crohn's disease, and reclassification of eggerthella hongkongensis lau et al. 2006 as paraeggerthella hongkongensis gen. nov., comb. nov. | a strictly anaerobic, gram-positive, short-rod/coccobacillus-shaped bacterial strain, designated 7-10-1-b(t), was isolated from the colon of a patient suffering from acute crohn's disease. the isolate formed small, pale-white, semi-translucent colonies on solid cultivation media. the strain was catalase-positive and metabolized only a small number of carbon sources. whole-cell fatty acids consisted predominantly of saturated fatty acids (89 %), of which 15 : 0 anteiso was the major component. th ... | 2009 | 19502325 |