| further complexity of the genus crinivirus revealed by the complete genome sequence of lettuce chlorosis virus (lcv) and the similar temporal accumulation of lcv genomic rnas 1 and 2. | the sequence of lettuce chlorosis virus (lcv) (genus crinivirus) was determined and found to contain unique open reading frames (orfs) and orfs similar to those of other criniviruses, as well as 3' non-coding regions that shared a high degree of identity. northern blot analysis of rna extracted from lcv-infected plants identified subgenomic rnas corresponding to six prominent internal orfs and detected several novel lcv-single stranded rna species. virus replication in tobacco protoplasts was in ... | 2009 | 19481773 |
| two crinivirus-specific proteins of lettuce infectious yellows virus (liyv), p26 and p9, are self-interacting. | interactions of lettuce infectious yellows virus (liyv)-encoded proteins were tested by yeast-two-hybrid (y2h) assays. liyv-encoded p34, hsp70h, p59, cp, cpm, and p26 were tested in all possible pairwise combinations. interaction was detected only for the p26-p26 combination. p26 self-interaction domains were mapped using a series of n- and c-terminal truncations. orthologous p26 proteins from the criniviruses beet pseudoyellows virus (bpyv), cucurbit yellow stunting disorder virus (cysdv), and ... | 2009 | 19665507 |
| host range and complete genome sequence of cucurbit chlorotic yellows virus, a new member of the genus crinivirus. | cucurbit chlorotic yellows virus (ccyv) causes chlorotic yellows on cucumber (cucumis sativus) and melon (cucumis melo) and is transmitted by bemisia tabaci biotype b and q whiteflies. to characterize the host range of ccyv, 21 cucurbitaceous and 12 other plant species were inoculated using whitefly vectors. all tested cucumis spp. except cucumis anguria and cucumis zeyheri were systemically infected with ccyv, although infection rates varied among species. citrullus lanatus, cucurbita pepo, and ... | 2010 | 20465411 |
| acquisition of lettuce infectious yellows virus by bemisia tabaci perturbs the transmission of lettuce chlorosis virus. | viruses in the genus crinivirus infect diverse plant species and are transmitted by specific whitefly vectors, but the basis for vector specific transmission remains poorly understood. here, we demonstrated that purified virion preparations of lettuce chlorosis virus (lcv) contained filamentous particles that were consistently transmitted to plants by whiteflies (bemisia tabaci biotypes a and b) following membrane feeding, suggesting that the preparations contained biologically active virions wi ... | 2011 | 21211541 |
| replication of lettuce chlorosis virus (lcv), a crinivirus in the family closteroviridae, is accompanied by the production of lcv rna 1-derived novel rnas. | cloned infectious complementary dnas of the bipartite genomic rnas of lettuce chlorosis virus (lcv) were constructed. inoculation of tobacco protoplasts with the in vitro produced rnas 1 and 2 transcripts, or with rna 1 transcript alone, resulted in viral replication accompanied by the production of novel lcv rna 1-derived rnas. they included the abundantly accumulating lm-lcvr1-1 (~0.38 kb) and lm-lcvr1-2 (~0.3 kb), and the lowly accumulating hm-lcvr1-1 (~8.0 kb) and hm-lcvr1-2 (~6.6 kb), all o ... | 2011 | 21945036 |
| a 3'-end structure in rna2 of a crinivirus is essential for viral rna synthesis and contributes to replication-associated translation activity. | the terminal ends in the genome of rna viruses contain features that regulate viral replication and/or translation. we have identified a y-shaped structure (yss) in the 3' terminal regions of the bipartite genome of lettuce chlorosis virus (lcv), a member in the genus crinivirus (family closteroviridae). the yss is the first in this family of viruses to be determined using selective 2'-hydroxyl acylation analyzed by primer extension (shape). using luciferase constructs/replicons, in vivo and in ... | 2016 | 27694962 |
| agroinoculation of the cloned infectious cdnas of lettuce chlorosis virus results in systemic plant infection and production of whitefly transmissible virions. | lettuce chlorosis virus (lcv) is a single stranded, positive strand rna virus that is solely transmitted by specific whitefly vectors (bemisia tabaci biotypes a and b) but not by mechanical leaf-rub inoculation. the roles of viral encoded proteins involved in the infection cycle of lcv have not yet been characterized due to the lack of reverse genetic tools. we present here a report of the successful development of an agrobacterium-mediated inoculation system for the cloned cdna constructs of lc ... | 2012 | 22926259 |
| lettuce chlorosis virus p23 suppresses rna silencing and induces local necrosis with increased severity at raised temperatures. | rna silencing functions as an antivirus defense strategy in plants, one that plant viruses counter by producing viral suppressors of rna silencing (vsrs). vsrs have been identified in three members of the genus crinivirus but they do not all share identical suppression mechanisms. here, we used agrobacterium co-infiltration assays to investigate the suppressor activity of proteins encoded by lettuce chlorosis virus (lcv). of 7 lcv proteins (1b, p23, hsp70 homolog, p60, cp, cpm, and p27) tested f ... | 2016 | 26828232 |