| binding of individual bacillus thuringiensis cry proteins to the olive moth prays oleae (lepidoptera: yponomeutidae). | the microlepidopteran prays oleae is one of the main insect pests causing significant crop losses in the mediterranean olive groves. bacillus thuringiensis based insecticides are being successfully used to minimize the impact of the second and third generations of this pest. however, because of its very small size and difficulty of rearing, very few studies have been carried out to determine the potency and mode of action of b. thuringiensis cry proteins in this insect. in this study, cry1ac, cr ... | 2009 | 19041324 |
| comparative study of bacillus thuringiensis cry1ia and cry1aa delta-endotoxins: activation process and toxicity against prays oleae. | cry1ia and cry1aa proteins exhibited toxicities against prays oleae with lc(50) of 189 and 116 ng/cm(2), respectively. the ability to process cry1ia11 protoxin by trypsin, chymotrypsin and p. oleae larvae proteases was studied and compared to that of cry1aa11. after solubilization under high alkaline condition (50mm naoh), cry1aa11 was converted into a major fragment of 65 kda, whereas cry1ia11 protoxin was completely degraded by p. oleae larvae proteases and trypsin and converted into a major f ... | 2010 | 20123104 |
| electrospun micro/nanofibers as controlled release systems for pheromones of bactrocera oleae and prays oleae. | new systems for the controlled release of 1,7-dioxaspiro[5.5]undecane and (z)-7-tetradecenal, the sex pheromones of olive fruit fly, bactrocera oleae, and olive moth, prays oleae, respectively, were developed utilizing electrospun micro/nanofiber matrices from inexpensive, biodegradable polymers, namely polycaprolactone, cellulose acetate and polyhydroxybutyrate. the incorporation of the pheromones in 5, 10 and 20% w/w in the electrospinning polymer blends allowed for the production of fiber mat ... | 2017 | 28271344 |
| is ground cover vegetation an effective biological control enhancement strategy against olive pests? | ground cover vegetation is often added or allowed to generate to promote conservation biological control, especially in perennial crops. nevertheless, there is inconsistent evidence of its effectiveness, with studies reporting positive, nil or negative effects on pest control. this might arise from differences between studies at the local scale (e.g. orchard management and land use history), the landscape context (e.g. presence of patches of natural or semi-natural vegetation near the focal orch ... | 2015 | 25646778 |
| factors affecting male prays oleae (lepidoptera: yponomeutidae) captures in pheromone-baited traps in olive orchards. | effects of trap design, height and side of trap placement on olive trees, pheromone doses in dispensers, aging of dispensers in the field, and secondary pheromone components were evaluated for the development of an effective pheromone monitoring system for prays oleae (bernard) lesne (lepidoptera: yponomeutidae) in olive orchards. field trials showed that trap design, pheromone dose, and trapping side affected male captures, whereas dispenser age, trap height, and secondary components had no inf ... | 2005 | 16334316 |
| fungal diversity associated to the olive moth, prays oleae bernard: a survey for potential entomopathogenic fungi. | olive production is one of the main agricultural activities in portugal. in the region of trás-os-montes, this crop has been considerably affected by prays oleae. in order to evaluate the diversity of fungi on prays oleae population of trás-os-montes olive orchards, larvae and pupae of the three annual generations (phyllophagous, antophagous and carpophagous) were collected and evaluated for fungal growth on their surface. from the 3,828 larvae and pupae, a high percentage of individuals exhibit ... | 2011 | 21994034 |
| prays oleae midgut putative receptor of bacillus thuringiensis vegetative insecticidal protein vip3lb differs from that of cry1ac toxin. | vegetative insecticidal protein (vip) is a class of insecticidal proteins produced by many bacillus thuringiensis strains during their vegetative growth stage. the vip3lb gene of b. thuringiensis strain bupm95, which encodes a protein active against the lepidoptera olive tree pathogenic insect prays oleae, was cloned into pet-14b vector and overexpressed in escherichia coli. the expressed vip3lb protein, found in the e. coli cytoplasmic fraction, was purified and used to produce anti-vip3lb anti ... | 2009 | 19434523 |
| proteolysis, histopathological effects, and immunohistopathological localization of delta-endotoxins of bacillus thuringiensis subsp. kurstaki in the midgut of lepidopteran olive tree pathogenic insect prays oleae. | considering the fact that prays oleae is one of the most pathogenic insects to the olive tree in the mediterranean basin, particularly in tunisia, the mode of action of cry insecticidal toxins of bacillus thuringiensis kurstaki in prays oleae midgut was investigated. the proteolysis of bacillus thuringiensis delta-endotoxins in the midgut was a key step in determining their potency against prays oleae. the latter's proteases activated the delta-endotoxins early, yielding stable toxins. the in vi ... | 2007 | 17435280 |
| evaluation of the effects, on canopy arthropods, of two agricultural management systems to control pests in olive groves from north-east of portugal. | this study aims to investigate the effect of management regime on canopy arthropod community of the olive tree (olea europaea l.). field studies were performed in two successive years, 2002 and 2003, in two olive groves, one under organic farming and the other under integrated protection. the integrated protection grove was sprayed once a year in june, with dimethoate, to control the anthophagous generation of the olive moth, prays oleae (bern.). from april to november of each year, the canopy a ... | 2007 | 17095048 |
| evidence of oral toxicity of photorhabdus temperata strain k122 against prays oleae and its improvement by heterologous expression of bacillus thuringiensis cry1aa and cry1ia genes. | photorhabdus temperata strain k122 exhibited oral toxicity against prays oleae with an lc50 of 58.1 x 10(6) cells ml(-1). recombinant p. temperata strains expressing the cry1aa and/or cry1ia genes of bacillus thuringiensis have been constructed. the two cry genes, encoding delta-endotoxins, were placed under the control of the lac promoter and iptg dependent expression in p. temperata was demonstrated. the presence of the cry genes in k122 resulted in a clear improvement of oral toxicity. this i ... | 2006 | 16413572 |
| evaluation of the effect on arthropod populations by using deltamethrin to control phloeotribus scarabaeoides bern. (coleoptera: scolytidae) in olive orchards. | field tests were made for the control of an olive insect pest, the olive bark beetle, phloeotribus scarabaeoides (col: scolytidae), using the pyrethroid insecticide deltamethrin (d) and this insecticide combined with ethylene (d+e), an attractant of the olive bark beetle. the tests were run in olive orchards in the provinces of granada and jaén (andalusia, southern spain), treating several olive rows so as to create a barrier effect to control the pest attack. the main objective was to evaluate ... | 2003 | 12729695 |
| developmental and reproductive biology of the ectoparasitoid, elasmus steffani, in a substitute host, ephestia kuehniella. | elasmus steffani (viggiani) (hymenoptera: elasmidae) is a gregarious idiobiont ectoparasitoid of prays oleae (bernard) (lepidoptera: plutellidae), an olive crop pest. in the substitute host ephestia kuehniella (zeller) (lepidoptera: pyralidae), the duration of the developmental stages was approximately 11-15 days. the preoviposition was 8.9 ± 5.0 days, and oviposition lasted 30.4 ± 10.5 days, with a reproduction capacity of 185.5 ± 62.3 eggs per female, for a mean of 5.4 ± 0.9 eggs per day. the ... | 2010 | 20874600 |
| evaluation of native plant flower characteristics for conservation biological control of prays oleae. | several studies have shown that manipulating flowering weeds within an agroecosystem can have an important role in pest control by natural enemies, by providing them nectar and pollen, which are significant sources of nutrition for adults. the aim of this study was to assess if the olive moth, prays oleae (bernard, 1788) (lepidoptera: praydidae), and five of its main natural enemies, the parasitoid species chelonus elaeaphilus silvestri (hymenoptera: braconidae), apanteles xanthostigma (haliday) ... | 2016 | 26780918 |