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aphid transmission of lettuce necrotic leaf curl virus, a member of a tentative new subgroup within the genus torradovirus.lettuce necrotic leaf curl virus (lnlcv) was described as the first non-tomato-infecting member of the genus torradovirus. until today, the virus was found only in the netherlands in two different areas in open field crops of lettuce. in 2015, lnlcv was accepted by the ictv as a new member of the genus torradovirus. the tomato-infecting (ti) torradoviruses tomato torrado virus (totv), tomato marchitez virus (tomarv) and tomato chocolàte virus (tochv) are transmitted by at least three whitefly sp ...201728223184
two generic pcr primer sets for the detection of members of the genus torradovirus.two degenerate primer pairs were designed for the universal detection of members of the genus torradovirus. primer pair torrado-1f/torrado-1r was designed based on the rna-dependent rna polymerase region located in rna1 and primer pair torrado-2f/torrado-2r based on a region overlapping the two first coat proteins vp35 and vp26 in rna2. the primers were used in two-step and one-step rt-pcr protocols. both primer pairs were able to detect 14 out of 15 isolates belonging to the two torradovirus sp ...201222771385
torradoviruses are transmitted in a semi-persistent and stylet-borne manner by three whitefly vectors.members of the genus torradovirus (family secoviridae, type species tomato torrado virus, totv) are spherical plant viruses transmitted by the whitefly species trialeurodes vaporariorum and bemisia tabaci. knowledge on the mode of vector transmission is lacking for torradoviruses. here, the mode of transmission was determined for tomato marchitez virus (tomarv). a minimal acquisition access period (aap) and inoculation access period (iap) of approximately 2h each was required for its transmissio ...201424342139
tomato chocolàte virus: a new plant virus infecting tomato and a proposed member of the genus torradovirus.a new virus was isolated from a tomato plant from guatemala showing necrotic spots on the bases of the leaves and chocolate-brown patches on the fruits. structural and molecular analysis showed the virus to be clearly related to but distinct from the recently described tomato torrado virus (totv) and tomato marchitez virus (tomarv), both members of the genus torradovirus. the name tomato chocolàte virus is proposed for this new torradovirus.201020229114
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