| cell-associated alpha-amylases of butyrate-producing firmicute bacteria from the human colon. | selected butyrate-producing bacteria from the human colon that are related to roseburia spp. and butyrivibrio fibrisolvens showed a good ability to utilize a variety of starches for growth when compared with the gram-negative amylolytic anaerobe bacteroides thetaiotaomicron. a major cell-associated amylase of high molecular mass (140-210 kda) was detected in each strain by sds-page zymogram analysis, and genes corresponding to these enzymes were analysed for two representative strains. amy13b fr ... | 2006 | 17074899 |
| transfer of conjugative elements from rumen and human firmicutes bacteria to roseburia inulinivorans. | studies on firmicutes bacteria from the gut are hampered by a lack of gene transfer systems. here the human colonic anaerobe roseburia inulinivorans a2-194 was shown to be a transfer recipient for two conjugative transposons, tn1545 from eubacterium cellulosolvens and tnk10 from clostridium saccharolyticum k10. | 2008 | 18456856 |
| in vitro kinetics of prebiotic inulin-type fructan fermentation by butyrate-producing colon bacteria: implementation of online gas chromatography for quantitative analysis of carbon dioxide and hydrogen gas production. | kinetic analyses of bacterial growth, carbohydrate consumption, and metabolite production of five butyrate-producing clostridial cluster xiva colon bacteria grown on acetate plus fructose, oligofructose, inulin, or lactate were performed. a gas chromatography method was set up to assess h2 and co2 production online and to ensure complete coverage of all metabolites produced. method accuracy was confirmed through the calculation of electron and carbon recoveries. fermentations with anaerostipes c ... | 2009 | 19633122 |