| staphylococcus devriesei sp. nov., isolated from teat apices and milk of dairy cows. | ten non-motile, gram-stain-positive, coagulase-negative staphylococci were isolated from bovine milk and teat apices. all isolates were catalase-positive, with anteiso-c(15 : 0), iso-c(15 : 0), anteiso-c(17 : 0), iso-c(17 : 0) and c(18 : 0) as predominant fatty acids and diphosphatidylglycerol and phosphatidylglycerol as major polar lipids. the results of sequence analysis of the 16s rrna gene and four housekeeping genes (rpob, hsp60, tuf and dnaj) in combination with trna-intergenic spacer leng ... | 2010 | 20061496 |
| cow teat skin, a potential source of diverse microbial populations for cheese production. | the diversity of the microbial community on cow teat skin was evaluated using a culture-dependent method based on the use of different dairy-specific media, followed by the identification of isolates by 16s rrna gene sequencing. this was combined with a direct molecular approach by cloning and 16s rrna gene sequencing. this study highlighted the large diversity of the bacterial community that may be found on teat skin, where 79.8% of clones corresponded to various unidentified species as well as ... | 2012 | 22081572 |
| coagulase-negative staphylococcus species in bulk milk: prevalence, distribution, and associated subgroup- and species-specific risk factors. | coagulase-negative staphylococci (cns) have become the main pathogens causing bovine mastitis in recent years. a huge variation in species distribution among herds has been observed in several studies, emphasizing the need to identify subgroup- and species-specific herd-level factors to improve our understanding of the differences in ecological and epidemiological nature between species. the use of bulk milk samples enables the inclusion of a large(r) number of herds needed to identify herd-leve ... | 2017 | 27865514 |
| teat apex colonization with coagulase-negative staphylococcus species before parturition: distribution and species-specific risk factors. | coagulase-negative staphylococci (cns) are the main cause of bovine intramammary infections and are also abundantly present in extramammary habitats such as teat apices. teat apex colonization (tac) with cns has already been explored in lactating dairy cows at the species level, whereas this is not true for dry cows and end-term heifers. therefore, the aim of this observational study was to describe cns tac in nonlactating dairy cows and end-term heifers in flemish dairy herds and to identify as ... | 2016 | 26709171 |
| staphylococcus petrasii sp. nov. including s. petrasii subsp. petrasii subsp. nov. and s. petrasii subsp. croceilyticus subsp. nov., isolated from human clinical specimens and human ear infections. | thirteen coagulase-negative, oxidase-negative, and novobiocin-susceptible staphylococci were isolated from human clinical specimens. the isolates were differentiated from known staphylococcal species on the basis of 16s rrna, hsp60, rpob, dnaj, tuf, and gap gene sequencing, automated ribotyping, (gtg)5-pcr fingerprinting, and maldi-tof ms analysis. phylogenetic analysis based on the 16s rrna gene sequence indicated phylogenetic relatedness of the analyzed strains to staphylococcus haemolyticus, ... | 2013 | 23318093 |