| detection of cryptosporidium oocysts in bivalve molluscs destined for human consumption. | clams (dosinia exoleta, ruditapes philippinarum, venerupis pullastra, venerupis rhomboideus, venus verrucosa), mussels (mytilus galloprovincialis), and oysters (ostrea edulis) were tested for the presence of cryptosporidium sp. oocysts using various stain techniques and a commercially available kit containing fluorescein isothiocyanate-conjugated monoclonal antibodies. all molluscs were harvested in northwest spain (galicia) except for r. philippinarum, which was from italy, and 1 of the 6 oyste ... | 2000 | 10958471 |
| norovirus, hepatitis a virus and enterovirus presence in shellfish from high quality harvesting areas in portugal. | this is the first report on the screening of shellfish from portugal for the presence of human enteropathogenic viruses. approximately 2000 shellfish (curbicula fluminea, ruditapes decussatus, tellina crassa, spisula solida, dosinia exoleta, ensis spp., mytilus spp., ostrea edulis and cerastoderma edule), organized in 49 batches, were collected between march 2008 and february 2009. they were tested for norovirus (nov), hepatitis a virus (hav) and enterovirus (ev) by rt-pcr followed by nucleotide ... | 2011 | 21569936 |
| determination of organochlorinated compounds in marine organisms by microwave-assisted extraction with molecular organized systems and liquid chromatography with fluorescence detection. | microwave-assisted extraction methodology is used to extract different compounds from various kinds of marine solid samples, such as soils, sediments, and organisms. a new analytical method was developed to extract polychlorinated biphenyls and polychlorinated dibenzofurans by using a conventional microwave system and the nonionic surfactant, polyoxyethylene 10 lauryl ether as the extractant as a prior step to liquid chromatography analysis coupled with fluorescence detection. the method was app ... | 2013 | 11878618 |
| subcellular distribution and trophic transfer of pb from bivalves to the common prawn palaemon serratus. | the edible clam dosinia exoleta has been reported to accumulate high contents of lead (pb) in soft tissues disregarding the levels of pb in the environment. this is due to the retention of pb in the form of metal rich granules (mrg) in their kidneys throughout the mollusc lifespan. the potential for trophic transfer of pb in this form to predators is expected to be low, since metals in the form of mrg are generally supposed to be trophically unavailable, but this assumption is based on studies w ... | 2017 | 28068582 |
| evolutionary dynamics of rdna clusters in chromosomes of five clam species belonging to the family veneridae (mollusca, bivalvia). | the chromosomal changes accompanying bivalve evolution are an area about which few reports have been published. to improve our understanding on chromosome evolution in veneridae, ribosomal rna gene clusters were mapped by fluorescent in situ hybridization (fish) to chromosomes of five species of venerid clams (venerupis corrugata, ruditapes philippinarum, ruditapes decussatus, dosinia exoleta, and venus verrucosa). the results were anchored to the most comprehensive molecular phylogenetic tree c ... | 2014 | 24967400 |
| surface spreading of synaptonemal complexes in the clam dosinia exoleta (mollusca, bivalvia). | there are only a few reports on the chromosomal location of dna sequences in bivalve species, none of them using meiotic chromosomes. mitotic chromosomes of the clam dosinia exoleta were analysed by means of giemsa, silver and fluorochrome staining and fluorescent in situ hybridization (fish) with 18s + 28s rdna and telomeric probes. a technique for surface spreading of synaptonemal complexes (scs) of dosinia exoleta was developed for the first time in a bivalve species. silver and dapi/pi stain ... | 2005 | 16170622 |