| a novel ph-stable, bifunctional xylanase isolated from a deep-sea microorganism, demequina sp. jk4. | a genomic library was constructed to clone a xylanase gene (mxyn10) from demequina sp. jk4 isolated from a deep sea. mxyn10 encoded a 471 residue protein with a calculated molecular mass of 49 kda. this protein showed the highest sequence identity (70%) with the xylanase from streptomyces lividans. mxyn10 contains a catalytic domain that belongs to the glycoside hydrolase family 10 (gh10) and a carbohydrate-binding module (cbm) belonging to family 2. the optimum ph and temperature for enzymatic ... | 2009 | 19884762 |
| Lysinimicrobium mangrovi gen. nov., sp. nov., a novel actinobacterium belonging to the family Demequinaceae isolated from mangrove rhizosphere soil. | A novel Gram-positive bacterium, designated HI08-69(T), was isolated from rhizosphere soil of mangrove from Iriomote Island, Japan, and its taxonomic position was investigated by a polyphasic approach. The peptidoglycan type of the isolate was A4a; lysine was the diagnostic diamino acid. The predominant menaquinone was demethylmenaquinone DMK-9(H(4)) and the major fatty acids were anteiso-C(15:0), anteiso-C(17:0) and C(16:0). The polar lipids comprised diphosphatidylglycerol, phosphatidylglycero ... | 2011 | 21930679 |
| demequina globuliformis sp. nov., demequina oxidasica sp. nov. and demequina aurantiaca sp. nov., actinobacteria isolated from marine environments, and proposal of demequinaceae fam. nov. | three gram-stain-positive actinobacterial strains, designated ym24-125(t), ym05-1041(t) and ym12-102(t), were isolated from marine environments. phylogenetic analysis based on 16s rrna gene sequences indicated that they belonged to the suborder micrococcineae and were most closely related to members of the genus demequina with 95.0-98.4 % sequence similarities. cells of strains ym05-1041(t) and ym12-102(t) were rod-shaped, similar to members of the genus demequina, while cells of strain ym24-125 ... | 2010 | 20656823 |
| demequina salsinemoris sp. nov., isolated on agar media supplemented with ascorbic acid or rutin. | three strains, kv-810(t), kv-811 and kv-816, were isolated from mangrove soil from a southern island in japan on media supplemented with ascorbic acid or rutin. these strains contained l-ornithine as the diagnostic diamino acid in the cell-wall peptidoglycan and dmk-9(h(4)) as the predominant menaquinone. the g+c content of the dna was 70-72 mol%. these characteristics in combination with 16s rrna gene sequence analysis revealed that the novel strains belonged to the genus demequina. the dna-dna ... | 2010 | 19667388 |
| demequina lutea sp. nov., isolated from a high arctic permafrost soil. | two gram-stain-positive, pigmented, non-motile, non-spore-forming, pleomorphic, rod-shaped bacteria (strains sv45(t) and sv47), isolated from a permafrost soil collected from the adventdalen valley, spitsbergen, northern norway, have been characterized taxonomically using a polyphasic approach. phylogenetic analysis based on 16s rrna gene sequences revealed that the two permafrost isolates formed a distinct phyletic line within the suborder micrococcineae of the order actinomycetales. dna-dna hy ... | 2009 | 19329581 |
| demequina aestuarii gen. nov., sp. nov., a novel actinomycete of the suborder micrococcineae, and reclassification of cellulomonas fermentans bagnara et al. 1985 as actinotalea fermentans gen. nov., comb. nov. | an actinobacterial strain containing demethylmenaquinone dmk-9(h(4)) as the diagnostic isoprenoid quinone was isolated from a tidal flat sediment sample, from south korea. phylogenetic analyses based on 16s rrna gene sequences showed that strain jc2054(t) represents a distinct phyletic line within the suborder micrococcineae of the order actinomycetales. the closest phylogenetic neighbour was cellulomonas fermentans, with 94.7 % 16s rrna gene sequence similarity. the novel isolate was strictly a ... | 2007 | 17220458 |