| factors influencing the production of cellulases by sporotrichum thermophile. | cellulase production and growth of a strain of sporotrichum thermophile were studied by using a mineral salts medium supplemented with yeast extract and insoluble cellulose. the effects of cultural conditions, such as ph, nitrogen source, substrate concentration, and temperature, were examined. maximum production of c1 and cx cellulases occurred at 45 c in 2 to 4 days, in the presence of 1% solka/floc as substrate, when nano3 or urea used as sources of nitrogen. under these conditions, celluloly ... | 1976 | 7194 |
| comparative temperature-stability properties of malate dehydrogenases from some thermophilic fungi. | temperature-activity and temperature-stability relationships of malate dehydrogenases from eight thermophilic fungi were determined. temperature optima for maximum activity of the enzymes varied between 50 degrees and 60 degrees and the arrhenius plots were linear between 5 degrees and 50 degrees. the energies of activation ranged from 2.1 kcal/mol for the enzyme from sporotrichum thermophile to 9.1 kcal/mol for that from penicillium duponti. heat inactivation kinetics at 50 degrees revealed hea ... | 1979 | 489257 |
| stimulation of growth and glucose catabolite enzymes by succinate in some thermophilic fungi. | thermophilic humicola lanuginosa, penicillium duponti, sporotrichum thermophile and mucor pusillus required succinate in addition to glucose for optimal growth. the requirement for succinate was concentration-dependent and the concentration needed for one half of the maximal growth was 6.14mm. in the presence of succinate, glucose utilization from the medium was markedly increased and this was associated with increased levels of the enzymes of the glycolytic and krebs cycle pathways. addition of ... | 1978 | 697502 |
| production of cellulolytic enzymes by immobilized sporotrichum thermophile. | spores of sporotrichum thermophile were immobilized in agar, polyacrylamide, and sodium alginate to generate in situ mycelium for production of cellulolytic enzymes. immobilized mycelium was considerably less effective than free cells for cellulase productivity. of the three gel types, agar beads proved to be the best carrier for the immobilized spores and subsequently generated mycelium. results of repeated batch experiments suggested that the immobilized mycelia could be reused but at much red ... | 1990 | 1366631 |
| fatal disseminated infection caused by myceliophthora thermophila, a new agent of mycosis: case history and laboratory characteristics. | we report a case of human infection caused by the hyphomycete myceliophthora thermophila. a 7-year-old male with neurofibromatosis (type 1) was diagnosed in 1987 with acute myeloblastic leukemia associated with the chromosomal abnormality monosomy 7. the patient experienced multiple serious infections over a three-year period before expiring in 1990 while in the end stage of leukemia. autopsy findings included fungal vegetations of the left atrium, ascending aorta, and pulmonary arteries and fun ... | 1992 | 1452676 |
| induction and regulation of alpha-amylase synthesis in a cellulolytic thermophilic fungus myceliophthora thermophila d14 (atcc 48104). | the alpha-amylase enzyme synthesis was higher when m. thermophila d-14 (atcc 48104) was grown in culture medium incorporated with starch or other carbohydrates containing maltose units. maximum enzyme production was attained with 1% starch followed by a gradual decrease with increasing concentration. marked decrease in alpha-amylase synthesis occurred with the addition of glucose to the culture medium and this decreasing activity was proportional to the concentration of glucose. the enzyme synth ... | 1992 | 1506028 |
| fungal flora associated with combine harvester wheat and sorghum dusts from egypt. | 107 species and 8 species varieties belonging to 44 genera were collected from combine harvester wheat and sorghum dusts (35 genera and 91 species + 4 varieties) and from the atmosphere of their hay sites (26 genera and 69 species + 4 varieties) on glucose- and cellulose-czapek's dox agar at 28 degrees c and 45 degrees c. the mycoflora of wheat and sorghum dusts were basically similar on the two types of media and the most common fungi were: alternaria alternata, aspergillus flavus, a. fumigatus ... | 1990 | 2266490 |
| purification and properties of an extracellular endoglucanase from myceliophthora thermophila d-14 (atcc 48104). | an extracellular endoglucanase (1,4-beta-glucanohydrolase, ec 3.2.1.4) produced by myceliophthora thermophila d-14 (atcc 48104) has been purified to homogeneity by ammonium sulphate precipitation and two consecutive ion-exchange chromatographic steps on deae-sephadex a-50 columns. the enzyme was purified 13.8-fold and was homogeneous by analytical page and sds-page. it has a high apparent mr, of about 100,000. the ph and temperature optima for its activity were 4.8 and 65 degrees c respectively. ... | 1990 | 2269871 |
| studies on the mycoflora of aswan high dam lake, egypt: monthly variations. | fifty-one species and one variety appertaining to twenty one genera of mesophilic fungi were recovered from the monthly samples of marginal water (44 species, 1 variety and 18 genera) and submerged mud (78 species, 1 variety and 30 genera) of aswan high dam lake during the period from july 1985 to december 1986. the most common species were aspergillus fumigatus, a. flavus, a. terreus, a. niger and penicillium funiculosum. the highest fungal populations were almost detected either in october, in ... | 1990 | 2352135 |
| isolation and characterization of new thiol protease inhibitors estatins a and b. | new thiol protease inhibitors, estatins a and b, were isolated from the culture filtrate of myceliophthora thermophila m4323. the basic, water-soluble inhibitors were characterized as having an agmatine, trans-epoxysuccinic acid and l-phenylalanine or l-tyrosine moieties in the structure. the molecular formulas c18h25n5o5 and c18h25n5o6 for a and b were indicated by elemental analysis and fast atom bombardment ms. estatins were specific inhibitors against thiol proteases such as papain, ficin an ... | 1989 | 2793591 |
| isolation, purification and regeneration of protoplasts from sporotrichum thermophile conidiospores. | protoplasts of uniform size were prepared from mononucleated conidiospores of sporotrichum thermophile. conidia were preincubated in glucose yeast extract medium at 45 c for 4 h. the conidia were collected resuspended in buffer containing 0.6 m kcl (as stabilizer), and incubated with novozyme sp249 and cellulase cp at 37 c for 6 h. the protoplasts were separated from cell wall fragments and intact conidia by centrifugation over 50% sucrose. the purified protoplasts were regenerated in glucose ye ... | 1988 | 3254166 |
| [thermophilic myceliophthora thermophila decomposes cellulose]. | a thermophilic microscopic fungus was isolated from cattle rumen and identified as myceliophthora thermophila (apinis) van oorschot. the culture synthesized cellulolytic enzymes and xylanase when it was grown in media containing cellulase at 50 degrees c under the conditions of submerged cultivation. the morphological and physiological characteristics of the culture are described and its taxonomic position is discussed. | 1983 | 6685815 |
| evidence for alpha and mu class glutathione s-transferases in a number of fungal species. | 1. the glutathione s-transferases of the following fungal species have been affinity purified on glutathione-sepharose; yarrowia lipolytica (ncyc825), sporotrichum thermophile (atcc16479), talaromyces emersonii (atcc26914) and issatchenkia orientalis (cbs5147 and an isolate provided by prof. kumagai, kyoto university). 2. sds-page analysis of affinity purified fungal extracts, followed by western blots with polyclonal antisera to rat gsts, was performed and indicated the presence of alpha and mu ... | 1993 | 8448996 |
| a study of a series of recombinant fungal laccases and bilirubin oxidase that exhibit significant differences in redox potential, substrate specificity, and stability. | a series of fungal laccases (polyporus pinsitus, rhizoctonia solani, myceliophthora thermophila, scytalidium thermophilum) and one bilirubin oxidase (myrothecium verrucaria) have been studied to determine their redox potential, specificity, and stability. polyporus and rhizoctonia laccases possess potentials near 0.7-0.8 v (vs. nhe), while other oxidases have potentials near 0.5 v. it is observed that higher redox potential correlates with higher activity. by epr, no significant change in the ge ... | 1996 | 8597577 |
| posttraumatic gas-containing brain abscess caused by clostridium perfringens with unique simultaneous fungal suppuration by myceliophthora thermophila: case report. | gas-containing brain abscesses are rare, and the vast majority are caused by clostridium perfringens. significant simultaneous fungal infection in a bacterial abscess is even rarer. we present such a case and review the literature. | 1996 | 8938783 |
| the phytase subfamily of histidine acid phosphatases: isolation of genes for two novel phytases from the fungi aspergillus terreus and myceliophthora thermophila. | phytases catalyse the hydrolysis of phytate (myo-inositol hexakisphosphate) to myo-inositol and inorganic phosphate. in this study genes encoding novel phytases from two different filamentous fungi, aspergillus terreus strain 9a-1 and myceliophthora thermophila were isolated. the encoded phya phytase proteins show 60% (a. terreus) and 48% (m. thermophila) identity, respectively, to the phya of aspergillus niger and have 21-29% identity compared to other histidine acid phosphatases. all three phy ... | 1997 | 9025298 |
| characterization of the gene encoding an extracellular laccase of myceliophthora thermophila and analysis of the recombinant enzyme expressed in aspergillus oryzae. | a genomic dna segment encoding an extracellular laccase was isolated from the thermophilic fungus myceliophthora thermophila, and the nucleotide sequence of this gene was determined. the deduced amino acid sequence of m. thermophila laccase (mtl) shows homology to laccases from diverse fungal genera. a vector containing the m. thermophila laccase coding region, under transcriptional control of an aspergillus oryzae alpha-amylase gene promoter and terminator, was constructed for heterologous expr ... | 1997 | 9251203 |
| site-directed mutations in fungal laccase: effect on redox potential, activity and ph profile. | a myceliophthora thermophila laccase and a rhizoctonia solani laccase were mutated on a pentapeptide segment believed to be near the type-1 cu site. the mutation l513f in myceliophthora laccase and the mutation l470f in rhizoctonia laccase took place at a position corresponding to the type-1 cu axial methionine (m517) ligand in zucchini ascorbate oxidase. the triple mutations v509l,s510e,g511a in myceliophthora laccase and l466v,e467s,a468g in rhizoctonia laccase involved a sequence segment whos ... | 1998 | 9693103 |
| fatal aortic myceliophthora thermophila infection in a patient affected by cystic medial necrosis. | a 22-year-old italian woman developed fungal aortitis after cardiac surgery for aortic insufficiency. she experienced two episodes of septic embolization and subsequently underwent replacement of the aortic root and initial ascending aorta by a homograft. the lumina of the ascending aorta, aortic arch and the origin of the innominate artery were completely filled with vegetation. from the involved tissue the phaeoid thermophilic hyphomycete myceliophthora thermophila (apinis) van oorschot was is ... | 1998 | 9776822 |
| biophysical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): molecular size, glycosylation pattern, and engineering of proteolytic resistance. | phytases (myo-inositol hexakisphosphate phosphohydrolases) are found naturally in plants and microorganisms, particularly fungi. interest in these enzymes has been stimulated by the fact that phytase supplements increase the availability of phosphorus in pig and poultry feed and thereby reduce environmental pollution due to excess phosphate excretion in areas where there is intensive livestock production. the wild-type phytases from six different fungi, aspergillus niger, aspergillus terreus, as ... | 1999 | 9925554 |
| biochemical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): catalytic properties. | supplementation with phytase is an effective way to increase the availability of phosphorus in seed-based animal feed. the biochemical characteristics of an ideal phytase for this application are still largely unknown. to extend the biochemical characterization of wild-type phytases, the catalytic properties of a series of fungal phytases, as well as escherichia coli phytase, were determined. the specific activities of the fungal phytases at 37 degreesc ranged from 23 to 196 u. (mg of protein)-1 ... | 1999 | 9925555 |
| cloning and characterization of a thermostable cellobiose dehydrogenase from sporotrichum thermophile. | cellobiose dehydrogenase (cdh) is an extracellular hemoflavoenzyme produced by several wood-degrading fungi. cdh contains one heme b and one fad per molecule and oxidizes cellobiose to cellobionolactone in the presence of cytochrome c. in this report, a thermostable cdh from the thermophilic ascomycete sporotrichum thermophile has been purified, cloned, and characterized. the temperature optimum for this cdh reaction was 60 degrees c, and the activation energy for the reaction was 26.3 kj/mol. t ... | 1999 | 10328816 |
| comparison of fungal laccases and redox mediators in oxidation of a nonphenolic lignin model compound. | several fungal laccases have been compared for the oxidation of a nonphenolic lignin dimer, 1-(3, 4-dimethoxyphenyl)-2-(2-methoxyphenoxy)propan-1,3-diol (i), and a phenolic lignin model compound, phenol red, in the presence of the redox mediators 1-hydroxybenzotriazole (1-hbt) or violuric acid. the oxidation rates of dimer i by the laccases were in the following order: trametes villosa laccase (tvl) > pycnoporus cinnabarinus laccase (pcl) > botrytis cinerea laccase (bcl) > myceliophthora thermop ... | 1999 | 10347057 |
| a critical review of cellobiose dehydrogenases. | cellobiose dehydrogenase (cdh) is an extracellular enzyme produced by various wood-degrading fungi. it oxidizes soluble cellodextrins, mannodextrins and lactose efficiently to their corresponding lactones by a ping-pong mechanism using a wide spectrum of electron acceptors including quinones, phenoxyradicals, fe(3+), cu(2+) and triiodide ion. monosaccharides, maltose and molecular oxygen are poor substrates. cdh that adsorbs strongly and specifically to cellulose carries two prosthetic groups; n ... | 2000 | 10725534 |
| dioxygen reactivity of laccase: dependence on laccase source, ph, and anion inhibition. | a study was carried out on the dioxygen reactivity of the laccases from trametes villosa, rhizoctonia solani, myceliophthora thermophila, scytalidium thermophilum, and coprinus cinereus. at ph 5.5, these laccases showed an apparently constant km (about 20-50 microm) for o2 with either 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) or methyl syringate as the reducing substrate, in contrast to the kcat, which varied up to 100-fold. o2 reactivity of the trametes and myceliophthora laccases ... | 2001 | 11694062 |
| formation of bound residues of 8-hydroxybentazon by oxidoreductive catalysts in soil. | this study was performed to determine which oxidoreductive catalysts were most efficient in catalyzing the binding of 8-hydroxybentazon to soil humic substances. 8-hydroxybentazon was completely transformed by an oxidoreductive enzyme, laccase of myceliophthora thermophila, at ph 3.0-7.0 within 30 min. when abiotic catalysts, manganese(iv), iron(iii), and aluminum oxides were used in the same ph range, 8-hydroxybentazon was completely transformed only by manganese(iv) oxide (delta-mno2), but a r ... | 2002 | 12033819 |
| toxicological studies on laccase from myceliophthora thermophila expressed in aspergillus oryzae. | the bioindustrially produced enzyme laccase can be used in different technical and food applications to facilitate processes. it can be added to different oral care products such as mouthwash, toothpaste, mints, and gums to prevent halitosis. laccase, produced by submerged fermentation of aspergillus oryzae, containing a gene originating from myceliophthora thermophila, was subject to a series of toxicological tests to document its safety in use. it was not found to be mutagenic in the salmonell ... | 2002 | 12202045 |
| redox-mediated decolorization of synthetic dyes by fungal laccases. | laccases from the lignin-degrading basidiomycetes trametes versicolor, polyporus pinisitus and the ascomycete myceliophthora thermophila were found to decolorize synthetic dyes to different extents. differences were attributed to the specific catalytic properties of the individual enzymes and to the structure of the dyes. due to their higher oxidative capacities, the laccases from the two basidiomycetes decolorized dyes more efficiently than that of the ascomycete. the azo dye direct red 28, the ... | 2002 | 12226723 |
| amperometric biosensors based on recombinant laccases for phenols determination. | graphite (ge) or printed graphite electrode (pge) based biosensors containing recombinant fungal laccase polyporus pinsitus (rppl), and myceliophthora thermophila (rmtl) were developed. the enzymes were immobilized using bovine serum albumin and glutaraldehyde. at ph 5.5 and -0.1 v, the calibration graphs of ge based biosensors were hyperbolic if pyrocatechol was used. the concentration of substrate that results in 50% of steady-state response (ec(50)) was 0.7 mm and sensitivity (s) was 3.8 ma/m ... | 2003 | 12485779 |
| antimicrobial activity of acidic xylo-oligosaccharides produced by family 10 and 11 endoxylanases. | acidic oligosaccharides were obtained from birchwood xylan by treatment with a thermoascus aurantiacus family 10 and a sporotrichum thermophile family 11 endoxylanases. the main difference between the products liberated by xylanases of family 10 and 11 concerned the length of the products containing 4-o-methyl-d-glucuronic acid. the xylanase from t. aurantiacus liberate from glucuronoxylan an aldotetrauronic acid as the shortest acidic fragment in contrast with the enzyme from s. thermophile, wh ... | 2003 | 12568925 |
| functional expression of a fungal laccase in saccharomyces cerevisiae by directed evolution. | laccase from myceliophthora thermophila (mtl) was expressed in functional form in saccharomyces cerevisiae. directed evolution improved expression eightfold to the highest yet reported for a laccase in yeast (18 mg/liter). together with a 22-fold increase in k(cat), the total activity was enhanced 170-fold. specific activities of mtl mutants toward 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and syringaldazine indicate that substrate specificity was not changed by the introduced mutati ... | 2003 | 12571021 |
| purification and characterization of a feruloyl esterase from fusarium oxysporum catalyzing esterification of phenolic acids in ternary water-organic solvent mixtures. | an extracellular feruloyl esterase (fae-ii) from the culture filtrates of fusarium oxysporum f3 was purified to homogeneity by sp-sepharose, t-butyl-hic and sephacryl s-200 column chromatography. the protein corresponded to molecular mass and pi values of 27 kda and 9.9, respectively. the enzyme was optimally active at ph 7 and 45 degrees c. the purified esterase was fully stable at ph 7.0-9.0 and temperature up to 45 degrees c after 1 h incubation. determination of k(cat)/k(m) revealed that the ... | 2003 | 12668312 |
| structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and ph optimum. | beta-1,4-galactanases hydrolyze the galactan side chains that are part of the complex carbohydrate structure of the pectin. they are assigned to family 53 of the glycoside hydrolases and display significant variations in their ph and temperature optimum and stability. two fungal beta-1,4-galactanases from myceliophthora thermophila and humicola insolens have been cloned and heterologously expressed, and the crystal structures of the gene products were determined. the structures are compared to t ... | 2003 | 12761390 |
| biosynthesis of fructo-oligosaccharides by sporotrichum thermophile during submerged batch cultivation in high sucrose media. | biosynthesis of fructo-oligosaccharides (fos) was observed during growth of the thermophilic fungus sporotrichum thermophile on media containing high sucrose concentrations. submerged batch cultivation with the optimum initial sucrose concentration of 250 g/l allowed the production of 12.5 g fos/l. the fos mixture obtained was composed of three sugars, which were isolated by size-exclusion chromatography. they were characterized by acid hydrolysis and hplc as 1-kestose, 6-kestose and neokestose. ... | 2004 | 12802527 |
| spectroscopic characterization of the leu513his variant of fungal laccase: effect of increased axial ligand interaction on the geometric and electronic structure of the type 1 cu site. | a variety of spectroscopic techniques, combined with density functional calculations, are used to describe the electronic structure of the leu513his variant of the type 1 cu site in myceliophthora thermophila laccase. this mutation changes the type 1 cu from a blue to a green site. electron paramagnetic resonance (epr), optical absorption, circular dichroism, and magnetic circular dichroism (mcd) spectroscopies reveal that, relative to the trigonal planar blue type 1 cu site in wild-type fungal ... | 2003 | 12817956 |
| biochemical and catalytic properties of an endoxylanase purified from the culture filtrate of sporotrichum thermophile. | an endo-beta-1,4-xylanase (1,4-beta-d-xylan xylanoxydrolase, ec 3.2.1.8) present in culture filtrates of sporotrichum thermophile atcc 34628 was purified to homogeneity by q-sepharose and sephacryl s-200 column chromatographies. the enzyme has a molecular mass of 25,000 da, an isoelectric point of 6.7, and is optimally active at ph 5 and at 70 degrees c. thin-layer chromatography (tlc) analysis showed that endo-xylanase liberates mainly xylose (xyl) and xylobiose (xyl2) from beechwood 4-o-methyl ... | 2003 | 12932372 |
| colorimetric assays for biodegradation of polycyclic aromatic hydrocarbons by fungal laccases. | polycyclic aromatic hydrocarbons (pahs) are highly toxic organic pollutants widely distributed in terrestrial and aquatic environments. in the present work, 2 colorimetric assays for laccase-catalyzed degradation of pahs were developed based on studies of the oxidation of 12 aromatic hydrocarbons by fungal laccases from trametes versicolor and myceliophthora thermophila. using a sodium borohydride water-soluble solution, the authors could reduce the single product of laccase-catalyzed anthracene ... | 2002 | 14599353 |
| mode of action of family 10 and 11 endoxylanases on water-unextractable arabinoxylan. | microbial endo-beta-1,4-xylanases (exs, ec 3.2.1.8) belonging to glycanase families 10 and 11 differ in their action on water-unextractable arabinoxylan (wu-ax). wu-ax was incubated with different levels of a thermoascus aurantiacus family 10 and a sporotrichum thermophile family 11 endoxylanases. at 10 g l(-1) arabinoxylan, enzyme concentrations (ke values) needed to obtain half-maximal hydrolysis rates (v(max) values) were 4.4 nm for the xylanase from t. aurantiacus and 7.1 nm for the xylanase ... | 2003 | 14599595 |
| purification and characterization of a type b feruloyl esterase (stfae-a) from the thermophilic fungus sporotrichum thermophile. | a feruloyl esterase (stfae-a) produced by sporotrichum thermophile was purified to homogeneity. the purified homogeneous preparation of native stfae-a exhibited a molecular mass of 57.0+/-1.5 kda, with a mass of 33+/-1 kda on sds-page. the pi of the enzyme was estimated by cation-exchange chromatofocusing to be at ph 3.1. the enzyme activity was optimal at ph 6.0 and 55-60 degrees c. the purified esterase was stable at the ph range 5.0-7.0. the enzyme retained 70% of activity after 7 h at 50 deg ... | 2004 | 14615854 |
| production, characterization and application of a thermostable polygalacturonase of a thermophilic mould sporotrichum thermophile apinis. | the production of polygalacturonase (pgase) by sporotrichum thermophile apinis in stirred submerged fermentation (smf) was high in comparison with that in static conditions. yeast extract (0.25%) and citrus pectin (2%) at ph 7.0 and 45 degrees c supported a high enzyme production in flasks agitated at 200 rpm. an overall 1.75-fold enhancement in pgase production was achieved as a result of optimization. the enzyme was optimally active at ph 7.0 and 55 degrees c, and exhibited t(1/2) of 4 h at 65 ... | 2004 | 15182829 |
| evaluation of certain food additives and contaminants. | this report represents the conclusions of a joint fao/who expert committee convened to evaluate the safety of various food additives, with a view to recommending acceptable daily intakes (adis) and to prepare specifications for the identity and purity of food additives. the first part of the report contains a general discussion of the principles governing the toxicological evaluation of food additives (including flavouring agents) and contaminants, assessments of intake, and the establishment an ... | 2004 | 15354533 |
| comparison of mesophilic and thermophilic feruloyl esterases: characterization of their substrate specificity for methyl phenylalkanoates. | the active sites of feruloyl esterases from mesophilic and thermophilic sources were probed using methyl esters of phenylalkanoic acids. only 13 out of 26 substrates tested were significant substrates for all the enzymes. lengthening or shortening the aliphatic side chain while maintaining the same aromatic substitutions completely abolished activity for both enzymes, which demonstrates the importance of the correct distance between the aromatic group and the ester bond. maintaining the phenylpr ... | 2004 | 15639097 |
| nuclear amination catalyzed by fungal laccases: reaction products of p-hydroquinones and primary aromatic amines. | [reaction: see text] nuclear amination of p-hydroquinones with primary aromatic amines was catalyzed by fungal laccases (ec 1.10.3.2) from trametes spec. and myceliophthora thermophila. this is the first report of laccase-catalyzed synthesis of aminoquinones. incubation of two compounds with laccase in the presence of oxygen resulted in the formation of the corresponding monoaminated or diaminated quinones. no hydroquinonoids were formed. observed differences in the reaction courses for differen ... | 2005 | 15760179 |
| laccase-catalysed iodide oxidation in presence of methyl syringate. | the kinetics of potassium triiodide (ki(3)) formation during fungal laccase action was investigated in presence of methyl syringate (ms). the recombinant forms of polyporus pinsitus (rppl), myceliophthora thermophila (rmtl), coprinus cinereus (rccl), and rhizoctonia solani (rrsl) laccases were used. the triiodide formation rate reached 6.1, 5.5, 6.0, and 2.1 microm/min at saturated rppl, rccl, rrsl, and rmtl concentration, respectively, in acetate buffer solution ph 5.5 and in presence of 10 mic ... | 2005 | 16080184 |
| screening mutant libraries of fungal laccases in the presence of organic solvents. | reliable screening methods are being demanded by biocatalysts' engineers, especially when some features such as activity or stability are targets to improve under non-natural conditions (i.e., in the presence of organic solvents). the current work describes a protocol for the design of a fungal laccase-expressed in saccharomyces cerevisiae-highly active in organic cosolvents. a high-throughput screening assay based on abts (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)) oxidation was val ... | 2005 | 16103414 |
| enzymic production of aldopentauronic acid and use as a bioregulator in plant airlift bioreactors. | neutral and acidic oligosaccharides were obtained from birchwood xylan by treatment with an endoxylanase, family 11 class, from sporotrichum thermophile. the main acidic xylooligosaccharide (aldopentauronic acid) was separated from the hydrolysate by anion-exchange and size-exclusion chromatography and the structure was determined by 13c nmr spectroscopy. the aldopentauronic acid yield was 25% (w/w) of the total solubilized sugars. the addition of purified aldopentauronic acid at a concentration ... | 2003 | 16233470 |
| sporotrichum thermophile growth, cellulose degradation, and cellulase activity. | the activity of components of the extracellular cellulase system of the thermophilic fungus sporotrichum thermophile showed appreciable differences between strains; beta-glucosidase (ec 3.2.1.21) was the most variable component. although its endoglucanase (ec 3.2.1.4) and exoglucanase (ec 3.2.1.91) activities were markedly lower, s. thermophile degraded cellulose faster than trichoderma reesei. the production of beta-glucosidase lagged behind that of endoglucanase and exoglucanase. the latter ac ... | 1987 | 16347439 |
| induction and catabolite repression of beta-glucosidase synthesis in myceliophthora thermophila d-14 (= atcc 48104). | beta-glucosidase activity in myceliophthora thermophila d-14 (= atcc 48104) was inducible and was produced in culture filtrate during growth with various inducers, of which pnpg (p-nitrophenyl-beta-d-glucoside) was the most efficient. induction of beta-glucosidase also occurred when the organism was grown in medium supplemented with different carbon sources. carboxymethyl cellulose, cellobiose, and solka-floc were found effective for induction of enzyme biosynthesis. the addition of glucose to t ... | 1988 | 16347725 |
| studies on thermophilic cellulolytic fungi. | three thermophilic cellulolytic fungi, chaetomium thermophile var. coprophile, sporotrichum thermophile, and thermoascus aurantiacus were studied to determine the conditions for a high rate of cellulose degradation. the range of temperature over which good growth occurred was determined first in a temperature gradient incubator; the optimum temperature was then established in shake flask cultures. t. aurantiacus had the highest optimum growth temperature range (46 to 51 c), whereas s. thermophil ... | 1975 | 16350026 |
| efficiency of new fungal cellulase systems in boosting enzymatic degradation of barley straw lignocellulose. | this study examined the cellulytic effects on steam-pretreated barley straw of cellulose-degrading enzyme systems from the five thermophilic fungi chaetomium thermophilum, thielavia terrestris, thermoascus aurantiacus, corynascus thermophilus, and myceliophthora thermophila and from the mesophile penicillum funiculosum. the catalytic glucose release was compared after treatments with each of the crude enzyme systems when added to a benchmark blend of a commercial cellulase product, celluclast, d ... | 2006 | 16599567 |
| phytase production by thermophilic mold sporotrichum thermophile in solid-state fermentation and its application in dephytinization of sesame oil cake. | the phytase production by sporotrichum thermophile tlr50 was recorded on all the commonly used animal feed ingredients tested to varying degrees in solid-state fermentation. enzyme production increased to 180 u/g of dry moldy residue (dmr) in sesame oil cake at 120 h and 45 degrees c at the initial substrate-to-moisture ratio of 1:2.5 and aw of 0.95. supplementation of sesame oil cake with glucose and ammonium sulfate further enhanced phytase titer (282 u/g of dmr). an overall 76% enhancement in ... | 2006 | 16720904 |
| thermostability of native and pegylated myceliophthora thermophila laccase in aqueous and mixed solvents. | a commercial preparation of laccase (ec 1.10.3.2), cloned from myceliophthora thermophila and expressed in aspergillus oryzae (mtl), was purified and modified by conjugation with poly(ethylene glycol) (m(r) = 5000) and is labeled peg-mtl. native enzyme was found to have a molecular mass of 80 kda, as determined by gel filtration, and 110 kda, by sds-page. the oxidative dimerization of 2,6-dimethoxyphenol (dmp) to produce the corresponding dibenzoquinone was catalyzed by mtl in a manner comparabl ... | 2006 | 16848420 |
| a marked enhancement in phytase production by a thermophilic mould sporotrichum thermophile using statistical designs in a cost-effective cane molasses medium. | statistical optimization of phytase production by a thermophilic mould sporotrichum thermophile in a cost-effective cane molasses medium. | 2006 | 16882141 |
| severe osteomyelitis caused by myceliophthora thermophila after a pitchfork injury. | traumatic injuries occurring in agricultural settings are often associated with infections caused by unusual organisms. such agents may be difficult to isolate, identify, and treat effectively. | 2006 | 16961922 |
| combinatorial saturation mutagenesis by in vivo overlap extension for the engineering of fungal laccases. | combinatorial saturation mutagenesis -csm- is a valuable tool for improving enzymatic properties from hot-spot residues discovered by directed enzyme evolution or performing semi-rational studies. csm coupled to a reliable high-throughput screening assay -coefficient of variance below 10%- has been used to enhance turnover rates in the fungal laccase variant t2 from myceliophthora thermophila. the influence of the highly conserved pentapeptide 509-513 on the redox potential of blue-copper contai ... | 2006 | 17168677 |
| novel cephalosporins synthesized by amination of 2,5-dihydroxybenzoic acid derivatives using fungal laccases ii. | sixteen novel cephalosporins were synthesized by amination of 2,5-dihydroxybenzoic acid derivatives with the aminocephalosporins cefadroxil, cefalexin, cefaclor, and the structurally related carbacephem loracarbef using laccases from trametes sp. or myceliophthora thermophila. all products inhibited the growth of several gram positive bacterial strains in the agar diffusion assay, among them methicillin-resistant staphylococcus aureus and vancomycin-resistant enterococci. the products protected ... | 2007 | 17329882 |
| improved phytase production by a thermophilic mould sporotrichum thermophile in submerged fermentation due to statistical optimization. | culture variables affecting phytase production by a thermophilic mould sporotrichum thermophile in submerged fermentation were optimized. soluble starch, peptone, tween-80 and sodium phytate were identified by plackett-burman design as the most significant factors to affect phytase production. the 2(4) full factorial central composite design of response surface methodology was applied for optimizing the concentrations of the significant variables and to delineate their interactions. starch, twee ... | 2008 | 17350826 |
| cytotoxic polyketides containing tetramic acid moieties isolated from the fungus myceliophthora thermophila: elucidation of the relationship between cytotoxicity and stereoconfiguration. | five new polyketides that contain tetramic acids, myceliothermophins a-e, were isolated from the thermophilic fungus myceliophthora thermophila. two sets of 5-alkyl-5-hydroxyl (or 5-methoxyl)-1h-pyrrol-2(5h)-one diastereomers, myceliothermophins a/b and c/d, were separated as pure compounds by using silica-gel column chromatography and recycling reverse-phase high-performance liquid chromatography (rp-hplc). the relative configurations of the chiral centers in 5-alkyl-5-hydroxyl (or 5-methoxyl)- ... | 2007 | 17503417 |
| chemoenzymatic synthesis of feruloyl d-arabinose as a potential anti-mycobacterial agent. | the feruloyl esterase (stfaec) produced by sporotrichum thermophile transfered the feruloyl group to d: -arabinose using a mixture of n-hexane, t-butanol and water. about 45% conversion of d: -arabinose to the feruloylated derivative was achieved. the compound had an mic value against mycobacterium bovis bcg of 25 microg/ml. | 2007 | 17676274 |
| phytase production by a thermophilic mould sporotrichum thermophile in solid state fermentation and its potential applications. | phytase production by a thermophilic mould sporotrichum thermophile apinis was investigated in solid state fermentation (ssf) using sesame oil cake as the substrate. scanning electron microscopy of the fermented sesame oil cake revealed a dense growth of the mould with abundant conidia. glucose, ammonium sulphate and incubation period were identified as the most significant factors by plackett-burman design. the optimum values of the critical components determined by central composite design of ... | 2008 | 17681787 |
| structural characterisation by esi-ms of feruloylated arabino-oligosaccharides synthesised by chemoenzymatic esterification. | the chemoenzymatic synthesis of feruloylated arabino-oligosaccharides has been achieved, using a feruloyl esterase type c from sporotrichum thermophile (stfaec). the structure of the feruloylated products was confirmed by esi-ms(n). | 2007 | 17909492 |
| altering the laccase functionality by in vivo assembly of mutant libraries with different mutational spectra. | the generation of diversity for directed protein evolution experiments shows an important bottleneck in the in vitro random mutagenesis protocols. most of them are biased towards specific changes that eventually confer a predicted and conservative mutational spectrum, limiting the exploration of the vast protein space. the current work describes a simple methodology to in vivo recombine mutant libraries with different nucleotide bias created by in vitro methods. this in vivo assembly was based o ... | 2008 | 17932916 |
| an assessment of the relative contributions of redox and steric issues to laccase specificity towards putative substrates. | laccases catalyze the one-electron oxidation of a broad range of substrates coupled to the 4 electron reduction of o2 to h2o. phenols are typical substrates, because their redox potentials (ranging from 0.5 to 1.0 v vs. nhe) are low enough to allow electron abstraction by the t1 cu(ii) that, although a relatively modest oxidant (in the 0.4-0.8 v range), is the electron-acceptor in laccases. the present study comparatively investigated the oxidation performances of trametes villosa and myceliopht ... | 2008 | 18292878 |
| laccase-induced c-n coupling of substituted p-hydroquinones with p-aminobenzoic acid in comparison with known chemical routes. | fungal laccases (benzenediol:oxygen oxidoreductase, ec 1.10.3.2) from pycnoporus cinnabarinus and myceliophthora thermophila were used as biocatalysts for enzymatic reaction of halogen-, alkyl-, alkoxy-, and carbonyl-substituted p-hydroquinones (laccase substrates) with p-aminobenzoic acid (no laccase substrate). during this reaction, the laccase substrate was oxidized to the corresponding quinones, which react with p-aminobenzoic acid by amination of the laccase substrate. the different substit ... | 2008 | 18668239 |
| laccase-catalyzed carbon-nitrogen bond formation: coupling and derivatization of unprotected l-phenylalanine with different para-hydroquinones. | unprotected l-phenylalanine was derivatized by an innovative enzymatic method by means of laccases from pycnoporus cinnabarinus and myceliophthora thermophila. during the incubation of l-phenylalanine with para-hydroquinones using laccase as biocatalyst, one or two main products were formed. dependent on the substitution grade of the hydroquinones mono- and diaminated products were detected. differences of the used laccases are discussed. the described reactions are of interest for the derivatiz ... | 2009 | 18695937 |
| characterization of a hap-phytase from a thermophilic mould sporotrichum thermophile. | the phytase of sporotrichum thermophile was purified to homogeneity using acetone precipitation followed by ion-exchange and gel-filtration column chromatography. the purified phytase is a homopentamer with a molecular mass of approximately 456kda and pi of 4.9. it is a glycoprotein with about 14% carbohydrate, and optimally active at ph 5.0 and 60 degrees c with a t(1/2) of 16h at 60 degrees c and 1.5h at 80 degrees c. the activation energy of the enzyme reaction is 48.6kjmol(-1) with a tempera ... | 2009 | 19054669 |
| antioxidants determination with laccase. | the spectrophotometric method of antioxidants determination using recombinant laccase polyporus pinsitus (rppl) and myceliophthora thermophila (rmtl) was developed. the method includes simultaneous oxidation of the antioxidant and high reactive laccase substrate producing chromophoric radical cation. as laccase substrates abts and other high reactive phenoxazine derivatives: 2-phenoxazin-10-yl-ethanol (pet), 3-phenoxazin-10-yl-propane-1-sulfonic acid (ppsa) and 3-phenoxazin-10-yl-propionic acid ... | 2007 | 19071650 |
| combinatorial saturation mutagenesis of the myceliophthora thermophila laccase t2 mutant: the connection between the c-terminal plug and the conserved (509)vsg(511) tripeptide. | a mutant laccase from the ascomycete myceliophthora thermophila has been submitted to iterative cycles of combinatorial saturation mutagenesis through in vivo overlap extension in saccharomyces cerevisiae. over 180,000 clones were explored, among which the s510g mutant revealed a direct interaction between the conserved (509)vsg(511) tripeptide, located in the neighborhood of the t1 site, and the c-terminal plug. the k(m)(o)(2) value of the mutant increased 1.5-fold, and the electron transfer pa ... | 2008 | 19075602 |
| left ventricular assist device outflow cannula obstruction by the rare environmental fungus myceliophthora thermophila. | left ventricular assist devices are used to provide mechanical circulatory support during end-stage heart failure either as a destination therapy or as a bridge to heart transplantation. perioperative transesophageal echocardiography is becoming an invaluable tool to investigate device function during implantation and in case of mechanical malfunction. most malfunctions are due to inflow graft occlusion, or device malfunction, while outflow graft dysfunction is rare. here, we present a case of s ... | 2009 | 19095833 |
| phytase production by sporotrichum thermophile in a cost-effective cane molasses medium in submerged fermentation and its application in bread. | phytase production by sporotrichum thermophile in a cost-effective cane molasses medium in submerged fermentation and its application in bread. | 2008 | 19120634 |
| plant growth promotion by an extracellular hap-phytase of a thermophilic mold sporotrichum thermophile. | phytase of the thermophilic mold sporotrichum thermophile apinis hydrolyzed and liberated inorganic phosphate from ca(+2), mg(+2), and co(+2) phytates more efficiently than those of al(3+), fe(2+), fe(3+), and zn(2+). the hydrolysis rate was higher at 60 degrees c as compared to 26 degrees celsius. among all the organic acids tested, citrate was more effective in enhancing solubilization of insoluble phytate salts by phytase than others. the dry weight and inorganic phosphate contents of the whe ... | 2010 | 19333564 |
| purification, characterization and mass spectrometric sequencing of a thermophilic glucuronoyl esterase from sporotrichum thermophile. | the cellulolytic system of the thermophilic fungus sporotrichum thermophile contains a recently discovered esterase that may hydrolyze the ester linkage between the 4-o-methyl-d-glucuronic acid of glucuronoxylan and lignin alcohols. the glucuronoyl esterase named stge1 was purified to homogeneity with a molecular mass of m(r) 58 kda and pi 6.7. the enzyme activity was optimal at ph 6.0 and 60 degrees c. the esterase displayed a narrow ph range stability at ph 8.0 and retained 50% of its activity ... | 2009 | 19459957 |
| an unusual ring--a opening and other reactions in steroid transformation by the thermophilic fungus myceliophthora thermophila. | a series of steroids (progesterone, testosterone acetate, 17beta-acetoxy-5 alpha-androstan-3-one, testosterone and androst-4-en-3,17-dione) have been incubated with the thermophilic ascomycete myceliophthora thermophila cbs 117.65. a wide range of biocatalytic activity was observed with modification at all four rings of the steroid nucleus and the c-17beta side-chain. this is the first thermophilic fungus to demonstrate the side-chain cleavage of progesterone. a unique fungal transformation was ... | 2009 | 19482085 |
| oxidation of the erythro and threo forms of the phenolic lignin model compound 1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)-1,3-propanediol by laccases and model oxidants. | mixtures of equal amounts of the erythro and threo forms of the phenolic arylglycerol beta-aryl ether 1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)-1,3-propanediol were oxidized (i) with laccases from trametes versicolor, agaricus bisporus, myceliophthora thermophila and rhus vernicifera, (ii) with laccase-mediator systems consisting of t. versicolor laccase and abts or hbt, and (iii) with various model oxidants including cerium(iv) ammonium nitrate (can), lignin peroxidase, fenton's reagen ... | 2009 | 19646732 |
| synthesis of model morpholine derivatives with biological activities by laccase-catalysed reactions. | the efficient enzyme-catalysed reaction of morpholines as model structures for bioactive compounds with para-dihydroxylated aromatic systems was carried out using the oxidoreductase laccase and atmospheric oxygen to produce eight novel morpholine-substituted aromatics. the laccase of myceliophthora thermophila was used for cross-linking morpholines containing primary or secondary amino groups with para-dihydroxylated laccase substrates. we demonstrate that not only primary amino groups, but also ... | 2009 | 19788410 |
| polymerization of lignosulfonates by the laccase-hbt (1-hydroxybenzotriazole) system improves dispersibility. | the ability of laccases from trametes villosa (tvl), myceliophthora thermophila (mtl), trametes hirsuta (thl) and bacillus subtilis (bsl) to improve the dispersion properties of calcium lignosulfonates 398 in the presence of hbt as a mediator was investigated. size exclusion chromatography showed an extensive increase in molecular weight of the samples incubated with tvl and thl by 107% and 572% from 28400 da after 17h of incubation, respectively. interestingly, ftir spectroscopy, (13)c nmr and ... | 2010 | 20176477 |
| derivatization of the azole 1-aminobenzotriazole using laccase of pycnoporus cinnabarinus and myceliophthora thermophila: influence of methanol on the reaction and biological evaluation of the derivatives. | the laccases of pycnoporus cinnabarinus and myceliophthora thermophila are extracellular enzymes with high protein stability. they were used for the 'one pot' synthesis of azole derivatives from 1-aminobenzotriazole together with the p-dihydroxylated laccase substrates 2,5-dihydroxybenzoic acid methyl ester and 2,5-dihydroxybenzoic acid ethyl ester. the reactions yielded heteromolecular dimers (in yields of up to 34%). methanol was used as the co-solvent to determine the influence of solvent con ... | 2010 | 20441569 |
| functional expression of a thermophilic glucuronyl esterase from sporotrichum thermophile: identification of the nucleophilic serine. | a glucuronyl esterase (ge) from the thermophilic fungus sporotrichum thermophile, belonging to the carbohydrate esterase family 15 (ce-15), was functionally expressed in the methylotrophic yeast pichia pastoris. the putative ge gene ge2 from the genomic dna was successfully cloned in frame with the sequence for the saccharomyces cerevisiae alpha-factor secretion signal under the transcriptional control of the alcohol oxidase (aox1) promoter and integrated in p. pastoris x-33 to confirm that the ... | 2010 | 20473662 |
| polyoxometalate/laccase-mediated oxidative polymerization of catechol for textile dyeing. | the synergistic effect between polyoxometalates (poms), namely k(5)[siw(11)v(v)o(40)]·11h(2)o and h(5)[pmo(10)v(v) (2)o(40)]·13h(2)o and laccase from ascomycete myceliophthora thermophila has been employed for the first time in oxidative polymerization of catechol. such a laccase-mediator system allowed the formation of a relatively high molecular weight polycatechol as confirmed by size exclusion chromatography and electrospray ionization mass spectrometry (esi-ms) (3990 da when using k(5)[siw( ... | 2010 | 20953600 |
| extracellular aldonolactonase from myceliophthora thermophila. | fungi secrete many different enzymes to deconstruct lignocellulosic biomass, including several families of hydrolases, oxidative enzymes, and many uncharacterized proteins. here we describe the isolation, characterization, and primary sequence analysis of an extracellular aldonolactonase from the thermophilic fungus myceliophthora thermophila (synonym sporotrichum thermophile). the lactonase is a 48-kda glycoprotein with a broad ph optimum. the enzyme catalyzes the hydrolysis of glucono-δ-lacton ... | 2010 | 21075873 |
| optimal culture conditions for keratinase production by a novel thermophilic myceliophthora thermophila strain gzuifr-h49-1. | aims: to investigate the effect of medium compositions and culture conditions on keratinase production by a novel thermophilic fungus myceliophthora thermophila (apinis) oorschot strain gzuifr-h49-1. methods and results: the thermophilic strain gzuifr-h49-1 with keratinolytic ability was characterized and identified as a strain of m. thermophila on the basis of its morphological characters and molecular analysis of its1-5.8s-its2 rdna sequence. among the medium compositions tested, the soluble ... | 2011 | 21241422 |
| a fatal case with disseminated myceliophthora thermophila infection in a lymphoma patient. | we report a lethal myceliophthora thermophila infection in an immunocompromised patient. based upon the clinical and mycological presentation, an initial diagnosis of invasive aspergillosis was made, possibly delaying optimal management in the patient. melanized fungi are emerging pathogens that require early identification to improve their unfavorable prognosis. | 2011 | 21429692 |
| towards industrially-feasible delignification and pitch removal by treating paper pulp with myceliophthora thermophila laccase and a phenolic mediator. | the ability of two natural phenols to act as mediators of the recombinant myceliophthora thermophila laccase (mtl) in eucalypt-pulp delignification was investigated. after alkaline peroxide extraction, the properties of the enzymatically-treated pulps improved with respect to the control. the pulp brightness increased (3.1 points) after the enzymatic treatment with mtl alone, but the highest improvements were obtained after the mtl treatment using syringaldehyde (4.7 points) and especially methy ... | 2011 | 21511459 |
| evaluation of the potential of fungal and plant laccases for active-packaging applications. | laccases from trametes versicolor (tvl), myceliophthora thermophila (mtl), and rhus vernicifera (rvl) were investigated with regard to their potential utilization as oxygen scavengers in active packages containing food susceptible to oxidation reactions. the substrate selectivity of the laccases was investigated with a set of 17 reducing substrates, mainly phenolic compounds. the temperature dependence of reactions performed at low temperatures (4-31 °c) was studied. furthermore, the laccases we ... | 2011 | 21524087 |
| invasive myceliophthora thermophila infection mimicking invasive aspergillosis in a neutropenic patient: a new cause of cross-reactivity with the aspergillus galactomannan serum antigen assay. | myceliophthora thermophila is a thermophilic mould widely found in the environment but rarely responsible for human infections. we describe a case of invasive myceliophthora thermophila infection mimicking invasive aspergillosis in a neutropenic patient with haematological malignancy. cross-reactivity with aspergillus galactomannan assay (gm) was demonstrated by repeated positive results and confirmed by cross-reaction between the fungal isolate and the gm assay. the patient was successfully tre ... | 2011 | 21619496 |
| in situ laccase-assisted overdyeing of denim using flavonoids. | a laccase-mediated system for denim overdyeing using phenolic compounds was developed. laccase from ascomycete myceliophthora thermophila was able to oxidize phenolic compounds such as catechol and catechin and mediate their attachment to denim surfaces. laccase-generated polymers gave rise to new coloration states from dark brown to green-yellow and replaced dyes in the overdyeing process. process parameters, such as enzyme dosage, incubation time and presence of mediator, were studied by consi ... | 2011 | 21751397 |
| recombinant expression, activity screening and functional characterization identifies three novel endo-1,4-+¦-glucanases that efficiently hydrolyse cellulosic substrates. | the hydrolysis of cellulose into fermentable sugars is a costly and rate-limiting step in the production of biofuels from renewable feedstocks. developing new cellulase systems capable of increased cellulose hydrolysis rates would reduce biofuel production costs. with this in mind, we screened 55 fungal endoglucanases for their abilities to be expressed at high levels by aspergillus niger and to hydrolyze amorphous cellulose at rates significantly greater than that obtained with trcel5a, one of ... | 2011 | 21710260 |
| Immobilisation of laccase on Eupergit supports and its application for the removal of endocrine disrupting chemicals in a packed-bed reactor. | Laccase from Myceliophthora thermophila was covalently immobilised on Eupergit C and Eupergit C 250L yielding specific activities of up to 17 and 80 U/g, respectively. Due to its superior activity, Eupergit C 250L was chosen for further research. The somewhat lower catalytic efficiency (based on the ratio between the turnover number and the Michaelis constant, k(cat)/K(M)) of the immobilised enzyme in comparison with that of the free enzyme was balanced by its increased stability and broader ope ... | 2011 | 22038613 |
| expression, characterization and structural modelling of a feruloyl esterase from the thermophilic fungus myceliophthora thermophila. | a ferulic acid esterase (fae) from the thermophilic fungus myceliophthora thermophila (synonym sporotrichum thermophile), belonging to the carbohydrate esterase family 1 (ce-1), was functionally expressed in methylotrophic yeast pichia pastoris. the putative fae from the genomic dna was successfully cloned in p. pastoris x-33 to confirm that the enzyme exhibits fae activity. the recombinant fae was purified to its homogeneity (39 kda) and subsequently characterized using a series of model substr ... | 2011 | 22012339 |
| oxidation of galactomannan by laccase plus tempo yields an elastic gel. | chemical modifications of galactomannans are applied to improve and/or modify their solubility, rheological and functional properties, but have limited specificity and are often difficult to control. enzymatic reactions, catalyzed under mild process conditions, such as depolymerization, debranching and oxidation, represent a viable and eco-friendly alternative. in this study, we describe oxidation of guar galactomannan primary hydroxyl groups by a fungal laccase using the stable radical tempo as ... | 2011 | 21903143 |
| comparative genomic analysis of the thermophilic biomass-degrading fungi myceliophthora thermophila and thielavia terrestris. | thermostable enzymes and thermophilic cell factories may afford economic advantages in the production of many chemicals and biomass-based fuels. here we describe and compare the genomes of two thermophilic fungi, myceliophthora thermophila and thielavia terrestris. to our knowledge, these genomes are the first described for thermophilic eukaryotes and the first complete telomere-to-telomere genomes for filamentous fungi. genome analyses and experimental data suggest that both thermophiles are ca ... | 2011 | 21964414 |
| immobilization of laccase by encapsulation in a sol-gel matrix and its characterization and use for the removal of estrogens. | laccase from myceliophthora thermophila was immobilized by encapsulation in a sol-gel matrix based on methyltrimethoxysilane and tetramethoxysilane. the amount of laccase used for the preparation of the hydrogel was in the range 2.2-22 mg of protein/ml sol and the corresponding enzymatic activities were in the range 5.5-17.0 u/g biocatalyst. the kinetic parameters of the encapsulated laccase showed that the immobilized enzyme presented lower affinity for the substrate 2,2'-azinobis-(3-ethylbenzo ... | 2011 | 21919219 |
| the structure of a novel glucuronoyl esterase from myceliophthora thermophila gives new insights into its role as a potential biocatalyst. | the increasing demand for the development of efficient biocatalysts is a consequence of their broad industrial applications. typical difficulties that are encountered during their exploitation in a variety of processes are interconnected with factors such as temperature, ph, product inhibitors etc. to eliminate these, research has been directed towards the identification of new enzymes that would comply with the required standards. to this end, the recently discovered glucuronoyl esterases (ges) ... | 2012 | 23275164 |
| effect of mutations on the thermostability of aspergillus aculeatus β-1,4-galactanase. | new variants of β-1,4-galactanase from the mesophilic organism aspergillus aculeatus were designed using the structure of β-1,4-galactanase from the thermophile organism myceliophthora thermophila as a template. some of the variants were generated using propka 3.0, a validated pka prediction tool, to test its usefulness as an enzyme design tool. the propka designed variants were d182n and s185d/q188t, g104d/a156r. variants y295f and g306a were designed by a consensus approach, as a complementary ... | 2015 | 25941560 |
| enhanced biological straw saccharification through coculturing of lignocellulose-degrading microorganisms. | lignocellulosic waste (lcw) is an abundant, low-cost, and inedible substrate for the induction of lignocellulolytic enzymes for cellulosic bioethanol production using an efficient, environmentally friendly, and economical biological approach. in this study, 30 different lignocellulose-degrading bacterial and 18 fungal isolates were quantitatively screened individually for the saccharification of four different ball-milled straw substrates: wheat, rice, sugarcane, and pea straw. rice and sugarcan ... | 2015 | 25724976 |
| development of genetic tools for myceliophthora thermophila. | the thermophilic filamentous fungus myceliophthora thermophila has many suitable characteristics for industrial biotechnology and could be a promising new chassis system for synthetic biology, particularly the atcc 42464 strain, whose genome was sequenced in 2011. however, metabolic engineering of this strain using genetic approaches has not been reported owing to a lack of genetic tools for this organism. | 2015 | 26013561 |
| selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation. | twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45°c on medium containing corn straw and cardboard as carbon sources. these fungi were identified in the genera aspergillus, thermomyces, myceliophthora, thermomucor and candida. the majority of the isolated strains produced xylanase and cellulases under solid state fermentation (ssf). the highest cellulase and xylanase productions wer ... | 2012 | 24031929 |
| enzymatic synthesis of model substrates recognized by glucuronoyl esterases from podospora anserina and myceliophthora thermophila. | glucuronoyl esterases (ges) are recently discovered enzymes that are suggested to cleave the ester bond between lignin alcohols and xylan-bound 4-o-methyl-d-glucuronic acid. although their potential use for enhanced enzymatic biomass degradation and synthesis of valuable chemicals renders them attractive research targets for biotechnological applications, the difficulty to purify natural fractions of lignin-carbohydrate complexes hampers the characterization of fungal ges. in this work, we repor ... | 2014 | 24531271 |
| box-behnken analysis and storage of spray-dried collagenolytic proteases from myceliophthora thermophila submerged bioprocess. | enzymes do not have long-term storage stability in soluble forms, thus drying methods could minimize the loss of enzymatic activity, the spray dryer removes water under high temperatures and little time. the aims of this study were to improve the stability of enzymatic extract from myceliophthora thermophila for potential applications in industry and to evaluate the best conditions to remove the water by spray drying technique. the parameters were tested according to box-behnken and evaluated by ... | 2017 | 28278111 |
| the role of the secondary coordination sphere in a fungal polysaccharide monooxygenase. | polysaccharide monooxygenases (pmos) are secreted metalloenzymes that catalyze the oxidative degradation of polysaccharides in a copper-, oxygen-, and reductant-dependent manner. cellulose-active fungal pmos degrade cellulosic substrates to be utilized as a carbon source for fungal growth. to gain insight into the pmo mechanism, the role of conserved residues in the copper coordination sphere was investigated. here, we report active-site hydrogen-bonding motifs in the secondary copper coordinati ... | 2017 | 28257189 |