| the ameliorating effects of the cognitive-enhancing chinese herbs on scopolamine-induced amnesia in rats. | ameliorating effects were investigated of the cognitive-enhancing chinese herbs administered orally for 1 week-panax ginseng (pg), panax notoginseng (png), dioscorea opposita (do), gastrodia elata (ge), salvia miltiorrhiza (sm), acorus gramineus (ag), coptis chinensis (cc), polygonum multiflorum (pm), cyperus rotundus (cr) and psoralea corylifolia (pc)-on the scopolamine (scop)-induced amnesia by using a passive avoidance task in rats. of ten chinese herbs, only pg, png, ge and cc prolonged the ... | 2000 | 10925408 |
| intracellular localization of a class iv chitinase from yam. | genomic dna encoding a class iv chitinase was cloned from yam (dioscorea opposita thunb) leaves in previous research (biosci. biotechnol. biochem., 68, 1508-1517 (2004)). but this chitinase had an additional sequence composed of eight amino acids (a c-terminal extension) at the c-terminal, compared with class iv chitianses from other plants. in order to clarify the role of this c-terminal extension in cellular localization, plants and suspension-cultured cells of nicotiana tabacum were transform ... | 2004 | 15277756 |
| a new phenanthrene glycoside and other constituents from dioscorea opposita. | phytochemical investigation of the rhizome of dioscorea opposita has led to the isolation of a new phenanthrene glycoside, 3,4,6-trihydroxyphenanthrene-3-o-beta-d-glucopyranoside (1), and five known compounds, soyacerebroside i (2), adenosine (3), beta-sitosterol (4), palmitic acid (5) and palmitoyloleoylphosphatidylcholine (6). their structures were determined by spectroscopic methods, including 1d- and 2d-nmr. compounds 1-6 exhibited no antifungal activity against the human pathogenic yeasts c ... | 2004 | 15467243 |
| [effects of extracts of chinese medicines on ganoderma lucidum in submerged culture]. | effects of water and ethanol extracts of 10 chinese medicines, such as astragalus membranaceus, coix lachryma-jobi, etc., on biomass and exopolysaccharide of ganoderma lucidum were studied by submerged culture. the results showed: water extracts of all medicines can improve the culture of g. lucidum except of a. membranaceus, ethanol extracts of c. lachryma-jobi, dioscorea opposita, codonopsis pilosula, and achyranthes bidentata( < 187.5g medicine/l substrate) can also increase the biomass of g. ... | 2003 | 16276930 |
| dioscorea opposita reverses dexamethasone induced insulin resistance. | the effects of dioscorea opposita (huai shan yao, hsy) on dexamethasone-induced insulin resistance were investigated in vitro and in vivo. d. opposita extract reduced significantly the blood insulin and glucose levels in dexamethasone-induced diabetic rats. in vitro, hsy significantly enhanced insulin-stimulated glucose uptake in 3t3-l1 adipocytes. moreover, hsy increase the mrna expression of glut4 glucose transporter in 3t3-l1 adipocytes. these data suggest that d. opposita has insulin sensiti ... | 2007 | 17084991 |
| modulation of activity of the adipocyte aquaglyceroporin channel by plant extracts. | the plasma membrane protein, aquaglyceroporin-7 (aqp7) is exclusively expressed in adipocytes and appears to be a channel for glycerol entry and exit. it is possible that by facilitating the opening of these channels, the loss of intracellular glycerol could be encouraged and thus reduce the size of the lipid reservoir. human preadipocytes and mouse 3t3-l1 preadipocytes were induced to develop an adipocytic phenotype by culture in a semi-defined medium. after 7 days, the expression of aqp7 messa ... | 2007 | 18489306 |
| neuroprotective effects of dioscorea opposita on scopolamine-induced memory impairment in in vivo behavioral tests and in vitro assays. | plants belong to the genus dioscorea have long been used as edible tuber crops in many tropical and subtropical areas and as a traditional herbal medicine in oriental countries including china, japan and korea. | 2009 | 19007874 |
| determination of diosgenin content in medicinal plants with enzyme-linked immunosorbent assay. | many medicinal plants contain diosgenin, which has a significant medicinal value. however, there is currently no effective and rapid analytical method to determine the diosgenin content of plants or products. in the present work we have developed an indirect competitive enzyme-linked immunosorbent assay (elisa) for the quantitative determination of diosgenin in herbal medicines. diosgenin was conjugated with bovine serum albumin (bsa) for immunization. a polyclonal antibody developed in rabbits ... | 2010 | 20549594 |
| complete genome sequence of chinese yam necrotic mosaic virus from dioscorea opposita in the republic of korea. | the complete genome sequence of chinese yam necrotic mosaic virus (chynmv) consisting of 8,213 nucleotides containing one open reading frame was determined by the transcriptome data generated from discorea opposita this is the first report of the complete nucleotide sequence of chynmv from dioscorea opposita in the republic of korea. | 2016 | 27492000 |
| characterization of a yam class iv chitinase produced by recombinant pichia pastoris x-33. | a yam (dioscorea opposita thunb) class iv chitinase, whose genomic dna was cloned by mitsunaga et al. (2004), was produced by the recombinant pichia pastoris x-33 in high yields such as 66 mg/l of culture medium. the chitinase was purified by column chromatography after endoglycosidase h treatment and then characterized. it showed properties similar to the original chitinase e purified from the yam tuber reported by arakane et al. (2000). this pichia-produced chitinase also showed strong lytic a ... | 2014 | 25036674 |
| molecular screening of chinese medicinal plants for progestogenic and anti-progestogenic activity. | estrogen and progestins have adverse effects, and many of these adverse effects are caused by progestins. due to this, many women choose to use botanical alternatives for hormone replacement therapy, which does not trigger steroidogenic properties. therefore, it is necessary to screen these herbs for progestogenic and anti-progestogenic properties. extract of 13 chinese medicinal plants were analysed for progestogenic and anti-progestogenic activities by using progesterone response element-drive ... | 2014 | 24845509 |
| the degradation, antioxidant and antimutagenic activity of the mucilage polysaccharide from dioscorea opposita. | the mucilage polysaccharide was extracted from dioscorea opposita in cold water and then degraded in two reagents hydrogen peroxide and ascorbic acid. three low-molecular-weight-samples were prepared, and their antioxidant and antimutagenic activity were investigated. chemical composition analysis indicated that the degradation action was in a concentration dependent manner. total sugars content of three degraded samples were significantly higher than raw sample. the uronic acid content in the d ... | 2016 | 27312633 |
| lethal hepatocellular necrosis associated with herbal polypharmacy in a patient with chronic hepatitis b infection. | following a short treatment for irritable bowel with the following herbs: astragalus propinquus, codonopsis pilosula, paeonia sp., atractylodes macrocephala, pueraria sp., poria cocos, dioscorea opposita, patriniae, psoralea corylifolia, alpinia katsumadai, glycyrrhiza uralensis and dolomiaea souliei sp. a 43-year-old woman developed acute severe liver failure requiring liver transplantation. histopathological examination of the liver showed massive hepatic necrosis in keeping with drug/chemical ... | 2014 | 24915453 |
| evaluation of starches obtained from four dioscorea species as binding agent in chloroquine phosphate tablet formulations. | starches obtained from four dioscorea species namely dioscorea dumetorum (bitter), dioscorea oppositifolia (chinese), dioscorea alata (water), and dioscorea rotundata (white) have been evaluated as binding agents in chloroquine phosphate tablet formulations in comparison with official corn starch. the compressional properties of the formulations were analyzed using density measurements and the heckel and kawakita equations. the mechanical properties of the tablets were assessed using tensile str ... | 2011 | 23960747 |
| development of repaglinide microspheres using novel acetylated starches of bitter and chinese yams as polymers. | tropical starches from dioscorea dumetorum (bitter) and dioscorea oppositifolia (chinese) yams were acetylated with acetic anhydride in pyridine medium and utilized as polymers for the delivery of repaglinide in microsphere formulations in comparison to ethyl cellulose. acetylated starches of bitter and chinese yams with degrees of substitution of 2.56 and 2.70 respectively were obtained. acetylation was confirmed by ftir, (1)h nmr spectroscopy. a 3(2) factorial experimental design was performed ... | 2017 | 27769931 |
| evaluation of the material and tablet formation properties of modified forms of dioscorea starches. | starches obtained from four different dioscorea species-namely, white yam (dioscorea rotundata), bitter yam (dioscorea dumetorum), chinese yam (dioscorea oppositifolia), and water yam (dioscorea alata)-were modified by cross-linking, hydroxypropylation, and dual modification-cross-linking followed by hydroxypropylation. the physicochemical, material, and tablet properties of the modified starches were investigated with the aim of understanding their properties to determine their potential use fo ... | 2009 | 19832640 |
| chemical components and emulsification properties of mucilage from dioscorea opposita thunb. | the properties of mucilage obtained from dioscorea opposita, generated during industrial manufacturing were investigated in this study. characteristics such as monosaccharide content, amino acid content, molecular weight, and structural features were measured, whereas morphology was observed using a scanning/transmission electron microscope. additionally, emulsification properties at different concentrations (0.2%, 0.4%, 0.6%, 0.8%, and 1.0%) and under acidic and basic ph (5.0 and 9.0) condition ... | 2017 | 28317729 |
| rheological properties of polysaccharides from dioscorea opposita thunb. | this study investigated the chemical components and rheological properties of polysaccharides from dioscorea opposita thunb. graded alcohol precipitation was used to extract dioscorea opposita polysaccharide samples (s1, s2, s3 and s4). the monosaccharides, amino acid content and molecular weight of each sample were measured and compared. the rheological properties of the polysaccharide samples at different concentrations, temperatures and ph values were studied. the rheological properties of s1 ... | 2017 | 28274459 |
| combination of morroniside and diosgenin prevents high glucose-induced cardiomyocytes apoptosis. | cornus officinalis and dioscorea opposita are two traditional chinese medicines widely used in china for treating diabetes mellitus and its complications, such as diabetic cardiomyopathy. morroniside (mor) of cornus officinalis and diosgenin (dio) of dioscorea opposita formed an innovative formula named m + d. the aims of the present study were to investigate myocardial protective effect of m + d on diabetic cardiomyopathy (dcm) through the inhibition of expression levels of caspase-3 protein, a ... | 2017 | 28106847 |
| emulsification properties of polysaccharides from dioscorea opposita thunb. | this study investigated the emulsification properties of polysaccharides from dioscorea opposita thunb. graded alcohol precipitation was used to extract dioscorea opposita polysaccharides fractions (4 samples) in different ranges of molecular weight. sample 3 contained more glucose and protein (80.13% and 0.34%, respectively), and molecular weight was approximately 34,790da, distributing narrowly. the droplet sizes and stabilities of emulsions made of gum arabic (ga) and polysaccharide samples a ... | 2017 | 27979295 |
| optimization of deep eutectic solvent-based ultrasound-assisted extraction of polysaccharides from dioscorea opposita thunb. | in this study, deep eutectic solvents were proposed for the ultrasound-assisted extraction of polysaccharides from dioscorea opposita thunb. several deep eutectic solvents were prepared for the extraction of polysaccharides, among which the deep eutectic solvent composed of choline chloride and 1,4-butanediol was proved to be suitable for the extraction. based on the screening of single-factor experiment design and orthogonal experiment design, three experimental factors were optimized for the b ... | 2017 | 27908714 |
| [study on rapid micropropagation in vitro technique of guangfeng medicinal yam (dioscorea opposita) plantlets]. | in order to provide methodology reference for virus-free and germplasm conservation of guangfeng medicinal yam (dioscorea opposita) plantlets, rapid micropropagation in vitro technique of guangfeng medicinal yam plantlets was studied. | 2015 | 27356371 |
| identification and validation of reference genes for qrt-pcr studies of gene expression in dioscorea opposita. | quantitative real-time polymerase chain reaction (qrt-pcr) is one of the most common methods for gene expression studies. data normalization based on reference genes is essential for obtaining reliable results for qrt-pcr assays. this study evaluated potential reference genes of chinese yam (dioscorea opposita thunb.), which is an important tuber crop and medicinal plant in east asia. the expression of ten candidate reference genes across 20 samples from different organs and development stages w ... | 2016 | 27314014 |
| [research on breeding of dioscorea opposita cv. tiegun]. | to breeding the new varieties dioscorea opposita cv. tiegun with the best comprehensive properties. | 2015 | 26930971 |
| ba-wei-die-huang-wan (hachimi-jio-gan) can ameliorate cyclophosphamide-induced ongoing bladder overactivity and acidic adenosine triphosphate solution-induced hyperactivity on rats prestimulated bladder. | ba-wei-die-huang-wan (bwdhw) is the traditional chinese medicine formula containing eight ingredients, namely rehmannia glutinosa (gaetn.) dc., root, steamed & dried; cornus officinalis siebold & zucc., fructus, dried; dioscorea oppositifolia l., root, dried; alisma plantago-aquatica, subsp. orientale (sam.) sam., tuber, dried; poria cocos (fr.) wolf., sclerotium, dried; paeonia×suffruticosa andrews, bark, dried; cinnamomum cassia (nees & t.nees) j. presl, bark, dried; aconitum carmichaeli debea ... | 2016 | 26719284 |
| anti-obesity effect of dioscorea oppositifolia extract in high-fat diet-induced obese mice and its chemical characterization. | dioscorea oppositifolia is a well-known edible and traditional medicine for the treatment of gastrointestinal diseases. in our previous study, d. oppositifolia exhibited both pancreatic lipase inhibition and an anti-adipogenesis effect in vitro. this study was performed to investigate the anti-obesity effect of d. oppositifolia on high-fat diet-induced obese mice. female icr mice were fed a high-fat diet with the 100 mg/kg of d. oppositifolia n-buoh extract for 8 weeks. the high-fat diet mice re ... | 2016 | 26700066 |
| a novel, stable, estradiol-stimulating, osteogenic yam protein with potential for the treatment of menopausal syndrome. | a novel protein, designated as doi, isolated from the chinese yam (dioscorea opposita thunb.) could be the first protein drug for the treatment of menopausal syndrome and an alternative to hormone replacement therapy (hrt), which is known to have undesirable side effects. doi is an acid- and thermo-stable protein with a distinctive n-terminal sequence gly-ile-gly-lys-ile-thr-thr-tyr-trp-gly-gln-tyr-ser-asp-glu-pro-ser-leu-thr-glu. doi was found to stimulate estradiol biosynthesis in rat ovarian ... | 2015 | 26160710 |
| in vivo protective effects of diosgenin against doxorubicin-induced cardiotoxicity. | doxorubicin (dox) induces oxidative stress leading to cardiotoxicity. diosgenin, a steroidal saponin of dioscorea opposita, has been reported to have antioxidant activity. our study was aimed to find out the protective effect of diosgenin against dox-induced cardiotoxicity in mice. dox treatment led to a significant decrease in the ratio of heart weight to body weight, and increases in the blood pressure and the serum levels of lactate dehydrogenase (ldh), creatine phosphokinase (cpk) and creati ... | 2015 | 26091236 |
| characterization and antihyperglycemic activity of a polysaccharide from dioscorea opposita thunb roots. | a polysaccharide dotp-80 from dioscorea opposita thunb was obtained by using the method of acid water-extraction and ethanol-precipitation. after being purified by chromatography, the structure characteristics of dotp-80 were established. based on the calibration curve obtained with standard dextrans, the molecular weight of the polysaccharide fraction dotp-80 was calculated to be 123 kda. the results of infrared spectrum (ft-ir) indicated that the polysaccharide contained the α-configuration of ... | 2015 | 25809611 |
| a comparison of near- and mid-infrared spectroscopic methods for the analysis of several nutritionally important chemical substances in the chinese yam (dioscorea opposita): total sugar, polysaccharides, and flavonoids. | the chinese yam (dioscorea opposita) is a basic food in asia and especially china. consequently, an uncomplicated, reliable method should be available for the analysis of the quality and origin of the yams. thus, near-infrared (nir) and mid-infrared (mid-ir) spectroscopic methods were developed to discriminate among chinese yam samples collected from four geographical regions. the yam samples were analyzed also for total sugar, polysaccharides, and flavonoids. these three analytes were used to c ... | 2015 | 25742643 |
| functional properties of autolysate and enzymatic hydrolysates from yam tsukuneimo (dioscorea opposita thunb.) tuber mucilage tororo: antioxidative activity and antihypertensive activity. | yam tsukuneimo tuber mucilage tororo hydrolysates were prepared by autolysis and three different peptic enzymes. except for pepsin hydrolysate, tororo was perfectly digested. each hydrolysate for 100 mg/ml significantly prolonged the induction period of auto-oxidation of linoleic acid, which was similar to 5 mm ascorbic acid. these hydrolysates also possessed high scavenging activities such as superoxide anion radicals, hydroxyl radicals, and dpph radicals. moreover, high antihypertensive activi ... | 2014 | 25477651 |
| changes in main nutrients and medicinal composition of chinese yam (dioscorea opposita) tubers during storage. | the changes in the main nutrient and medicinal components during the storage of the chinese yam (dioscorea opposita) tubers were studied. the harvested tubers were stored under ambient conditions (10 °c to 18 °c, 60 % to 80 % relative humidity) and cold temperature and packaged conditions (4 °c, 60 % to 65 % relative humidity) for 45 day. the allantoin, starch, total alcohol-soluble sugar, reducing sugar, protein, and moisture contents of the samples were evaluated. their amylase activities were ... | 2014 | 25328193 |
| anti-adipogenic constituents from dioscorea opposita in 3t3-l1 cells. | we previously reported the lipase inhibitory activity of the n-buoh fraction of dioscorea opposita (dob) and its isolates. this study sought to evaluate their anti-adipogenic activity in terms of their effects on the adipogenic transcription factors peroxisome proliferator-activated receptor γ (pparγ) and ccaat/enhancer binding protein α (c/ebpα) as well as phosphorylated amp-activated protein kinase (p-ampk) and carnitine palmitoyl transferase-1 (cpt-1). dob apparently attenuated 3t3-l1 adipocy ... | 2014 | 25273391 |
| integrating traditional chinese medicine healthcare into diabetes care by reducing the risk of developing kidney failure among type 2 diabetic patients: a population-based case control study. | our previous study indicated that the traditional chinese medicine (tcm) formula liu-wei-di-huang-wan, which consists of six type of herbs, namely rehmannia glutinosa (gaertn.) dc., root, dried; cornus officinalis siebold & zucc., fructus, dried; dioscorea oppositifolia l., root, dried; alisma plantago-aquatica subsp. orientale (sam.) sam., tuber, dried; paeonia × suffruticosa andrews, bark, dried; poria cocos (fr.) wolf., sclerotium, dried, is the most frequently prescribed herbal formula used ... | 2014 | 25178949 |
| a protocol for in vitro production of microtubers in chinese yam (dioscorea opposita). | chinese yam (dioscorea opposita) is an important tuberous crop owing to its dual use as a food as well as a medicine. tissue culture techniques allow the rapid multiplication of virus-free plant materials. the use of microtubers offers an attractive alternative to in vitro-grown plantlets for the micropropagation and exchange of healthy chinese yam materials. a protocol for the in vitro production of chinese yam microtubers was developed in this study. though we tested both one-step and two-step ... | 2014 | 25036126 |
| [evolution and change of the species quality and authentic producing areas of dioscorea opposita thunb]. | yam was first recorded in shen nong ben cao jing (shennong's classic of materia medica) which was basically came from dioscorea opposita since ancient time. before the tang dynasty, it basically came from the wild species of dioscorea opposita. in the song dynasty, d. opposita began to be cultivated, but chinese medical practitioners claimed that the wild species was better than the cultivated one. after the ming and qing dynasties, yam was mostly from cultivated resources. as the species qualit ... | 2014 | 24989805 |
| extraction of total dna and optimization of the rapd reaction system in dioscorea opposita thunb. | dioscorea opposita thunb. has been used as health food and herbal medicinal ingredients in traditional chinese medicine. in this study, the total dna of d. opposita thunb. was extracted using an improved cetyltrimethylammonium bromide (ctab) method, and the extracted dna was further used for random amplified polymorphic dna (rapd) reaction system by design of the l16 (4(4)) orthogonal diagram. the results showed that the improved ctab method can be used to isolate high-quality and high-concentra ... | 2014 | 24634232 |
| an effective method for extracting total rna from dioscorea opposita thunb. | dioscorea opposita thunb., included in the genus dioscorea of the family dioscoreaceae, is an important herb with great edible and medicinal value. in this study, the total rna from leaves of lichuan dioscorea opposita thunb. was isolated by an improved trizol method. the results showed that the rna extracted by the improved trizol method had good integrity, and the rna could be used for down-stream molecular biology operations including reverse transcription-polymerase chain reaction. | 2014 | 24535874 |
| antioxidant chinese yam polysaccharides and its pro-proliferative effect on endometrial epithelial cells. | chinese yam polysaccharide (cyp) was successfully isolated from the rhizoma of dioscorea opposita and its chemical characteristics were determined. antioxidant and pro-proliferative activity of cyp on human endometrial epithelial cells in vitro were investigated. role of cyp on cell proliferation was also evaluated by examining pro-apoptotic and anti-apoptotic proteins bax and bcl-2 by western blot analysis. chemical composition analysis indicated that cyp was mainly composed of mannose, glucose ... | 2014 | 24530337 |
| aqueous extract of dioscorea opposita thunb. normalizes the hypertension in 2k1c hypertensive rats. | dioscorea opposita thunb. (huai shan yao, dot), a common staple food in china, has been used for more than 2000 years in traditional chinese medicine (tcm) to treat different systemic diseases including hypertension. the objective of this study was to investigate the possible antihypertensive effects of the aqueous extract of (dot) in renovascular hypertensive rats as well as the mechanism in reducing blood pressure. | 2014 | 24447776 |
| new phenanthrene glycosides from dioscorea opposita. | two new phenanthrene glycosides, dioscopposide a and dioscopposide b (1 and 2), were isolated from the rhizomes of dioscorea opposita. their structures were determined primarily on the basis of 1d and 2d nmr techniques, ms studies, and chemical methods. all the isolates were evaluated for their inhibitory effects on the lipopolysaccharide-induced nitric oxide production using murine macrophage raw 264.7 cells. the ic50 values of dioscopposide a and dioscopposide b were 5.8 and 7.2 μm, respective ... | 2014 | 24168215 |
| neuroprotective effect of liuwei dihuang decoction on cognition deficits of diabetic encephalopathy in streptozotocin-induced diabetic rat. | liuwei dihuang decoction (lwdhd) is a well-known prescription of traditional chinese medicine (tcm) and consists of six crude drugs including rehmannia glutinosa libosch. (family: scrophulariaceae), cornus officinalis sieb. (family: cornaceae), dioscorea oppositifolia l. (family: dioscoreaceae), paoenia ostii (family: paeoniaceae), alisma orientale (g. samuelsson) juz (family: alismataceae) and poria cocos (schw.) wolf (family: polyporaceae). it has been used for the treatment of "kidney-yin" de ... | 2013 | 24041458 |
| production of yam mosaic virus (ymv)-free dioscorea opposita plants by cryotherapy of shoot-tips. | in the present study, yam mosaic virus (ymv) could be efficiently eliminated by cryotherapy in dioscorea opposita. shoot apices were precultured for 16 h with 0.3 m sucrose, encapsulated in sodium alginate and dehydrated for 4 h prior to direct immersion in liquid nitrogen. up to 90 percent of the plants regenerated from cryopreserved shoot tips were ymv-free, whereas only 40% of those regenerated using meristem culture were ymv-free. ymv-free yam plantlets could be propagated in vitro through n ... | 2013 | 23625083 |
| traditional chinese medicine syndrome-related herbal prescriptions in treatment of malignant tumors. | to investigate the distribution characteristics of tcm syndromes and the related herbal prescriptions for malignant tumors (mt). | 2013 | 23596807 |
| paleolithic human exploitation of plant foods during the last glacial maximum in north china. | three grinding stones from shizitan locality 14 (ca. 23,000-19,500 calendar years before present) in the middle yellow river region were subjected to usewear and residue analyses to investigate human adaptation during the last glacial maximum (lgm) period, when resources were generally scarce and plant foods may have become increasingly important in the human diet. the results show that these tools were used to process various plants, including triticeae and paniceae grasses, vigna beans, diosco ... | 2013 | 23509257 |
| a lectin with highly potent inhibitory activity toward breast cancer cells from edible tubers of dioscorea opposita cv. nagaimo. | a 70-kda galactose-specific lectin was purified from the tubers of dioscorea opposita cv. nagaimo. the purification involved three chromatographic steps: anion exchange chromatography on a q-sepharose column, fplc-anion exchange chromatography on a mono q column, and fplc-gel filtration on a superdex 75 column. the purified nagaimo lectin presented as a single 35-kda band in reducing sds-page while it exhibited a 70-kda single band in non-reducing sds-page suggesting its dimeric nature. nagaimo ... | 2013 | 23349827 |
| phenolic compounds with pancreatic lipase inhibitory activity from korean yam (dioscorea opposita). | abstract twenty-three phenolic compounds were isolated from dioscorea opposita by bioactivity-guided method and their inhibitory effect against pancreatic lipase was evaluated. a total of 15 isolates reduced lipase activity at ic50 values of less than 50 µm and 3,3',5-trihydroxy-2'-methoxybibenzyl showed the highest inhibition with an ic50 value of 8.8 µm. this study is a first to reveal the pancreatic lipase inhibitory activity by both d. opposita and its isolated compounds. | 2014 | 23327640 |
| [study of dioscorea opposita thunb from different producing areas with xrf and pxrd]. | the contents of various elements in rhizoma dioscoreae were analyzed and compared with those of shishou d. opposita thunb on the whole jointly by xrf and pxrd. the results showed that the element cu, al and mn could not be detected in rhizoma dioscoreae, but the element mg, zn and cr were found, and other elements contents changed to different degrees; there are starch and whewellite in both groups, the crystal type of starches separated from rhizoma dioscoreae cultivars was a typical b-type pat ... | 2012 | 23016366 |
| raw chinese yam (dioscorea opposita) promotes cecal fermentation and reduces plasma non-hdl cholesterol concentration in rats. | we examined the effects of raw chinese yam (dioscorea opposita), containing resistant starch (rs), on lipid metabolism and cecal fermentation in rats. raw yam (ry) and boiled yam (by) contained 33.9% and 6.9% rs, respectively. male sprague-dawley rats were fed a cholesterol-free, control (c) diet supplemented with or without 15 and 30 g of ry or by/100 g for 3 wk. plasma total cholesterol concentrations in the tail vein of rats fed the 30% ry diet were significantly lower than in the c group thr ... | 2011 | 22293211 |
| effect of dioscorea opposita thunb. (yam) supplementation on physicochemical and sensory characteristics of yogurt. | a study was conducted to examine the physicochemical, microbial, and sensory properties of yogurt made by supplementing powdered yam dioscorea opposita thunb. (ypt) at different concentrations (0.2, 0.4, 0.6, and 0.8%, wt/vol) into milk, which was pasteurized and then fermented at 43°c for 6 h and stored for 16 d. the ph values of all samples decreased, whereas viscosity values and mean microbial counts increased during storage. the l* and a* color values (indicators of lightness and redness, re ... | 2011 | 21426958 |
| phenolic compounds with radical scavenging and cyclooxygenase-2 (cox-2) inhibitory activities from dioscorea opposita. | phytochemical studies of the chloroform soluble fraction of dioscorea opposita resulted in the isolation of four new compounds, 3,5-dihydroxy-4-methoxybibenzyl (1), 3,3',5-trihydroxy-2'-methoxybibenzyl (2), 10,11-dihydro-dibenz[b,f]oxepin-2,4-diol (3), and 10,11-dihydro-4-methoxy-dibenz[b,f]oxepin-2-ol (4), together with an additional fifteen known compounds. the structures of 1-4 were elucidated by spectroscopic methods including 2d nmr. all of the nineteen isolated compounds were tested in the ... | 2009 | 19303782 |
| cryopreservation of plantlet nodes of dioscorea opposita thunb. using a vitrification method. | a cryopreservation method by vitrification was developed for long-term storage of dioscorea opposita thunb., a valuable native medicinal plant species in henan province of china. the cryopreservation protocol was established with cultivar b and evaluated with another four cultivars, tiegun, 47, taigu and huaiqing 1. the results showed that nodes with a bud excised from 60 d plantlets were desirable for the cryopreservation. the optimum procedure was established as: 1) the plantlets were cultured ... | 2013 | 19274308 |
| [studies on chemical constituents of dioscorea opposita]. | to study the chemical constituents of dioscorea opposita. | 2008 | 18831204 |
| [study on enzyme-assisted extraction of polysaccharides from dioscorea opposita]. | to extract polysaccharides from dioscorea opposita by alpha-amylase and ultrasound. | 2008 | 18533489 |
| functional properties of dioscorin, a soluble viscous protein from japanese yam (dioscorea opposita thunb.) tuber mucilage tororo. | a soluble viscous protein was purified from yam (dioscorea opposita thunb.) tuber mucilage tororo by chromatographic steps, and its functional properties were estimated. the purified dioscorin having the molecular weight of about 200 kda exhibited high scavenging activities against hydroxyl radicals (ic50 = 195.1 microg/ml) and superoxide anion radicals (ic50 = 92.7 microg/ml). moreover, it showed extremely high angiotensin i-converting enzyme inhibitory activity (ic50 = 41.1 microg/ml). the res ... | 2016 | 17294688 |
| structural characterization of a glucan isolated from hedyotis diffusa willd. | to isolate a homogeneous polysaccharide rp from rdp (a crude polysaccharide from dioscorea opposita thunb.), and study its preliminary com-position and structure. | 2006 | 17212045 |
| [research of polysaccharide from dioscorea opposita thunb]. | to isolate a homogeneous polysaccharide rp from rdp (a crude polysaccharide from dioscorea opposita thunb.) , and study its preliminary composition and structure. | 2006 | 17212044 |
| [determination of nine trace elements in dioscorea opposita thumb by flame atomic absorption spectrophotometry]. | the contents of the potassium, calcium, sodium, magnesium, copper, zinc, iron, manganese, strontium and nickel in dioscorea opposita thumb were determined. the relative standard deviation(rsd) of the first eight contents is 0.43%, 1.10%, 4.41%, 0.68%, 1.44%, 1.88%, 1.29% and 0.03% respectively, and the percentage recovery is 90.0%-111.0%. the method is convenient and accurate. it can be used to determine the trace elements simultaneousy in dioscorea opposita thumb. | 2006 | 16883880 |
| [study on model of heat and mass transfer in dioscorea opposita with microwave drying technique]. | a new mathematical model, aimed at investigating the behaviors of microwave drying of traditional chinese medicine was established. it based on mass-energy consersvation law. dioscorea opposita thunb. was used as sample and its drying efficiency have been developed at different levels of microwave power and sample thickness. the results shown that the levels of them have a strong influence on drying process. the experimental values were approximately in accord with the calculated values by mathe ... | 2005 | 16447865 |
| new starches from traditional chinese medicine (tcm)--chinese yam (dioscorea opposita thunb.) cultivars. | the starches separated from two different dioscorea opposita thunb. cultivars were investigated for morphological, thermal and crystal properties. the shape of starch granules separated from different d. opposita thunb. cultivars varied from round to oval or irregular. the surface of the starch granules appeared to be smooth without any fissures. the average particle diameter of starches from different d. opposita thunb. cultivars was 40.3 and 38.7mum for d. 47 and d. sxy starch, respectively. t ... | 2006 | 16325789 |
| [genetic diversity of yam (dioscorea opposita thunb) detected by issr markers]. | genetic diversity of 28 cultivars of yam (dioscorea opposita thunb) was assessed by means of inter-simple sequence repeat (issr) markers. the results showed that seven proper primers, with rich polymorphism, could be selected from a total of forty four issr ones; distinct differences appeared among 28 cultivars amplified bands, and the rate of polymorphic bands was 83.01%; shannon's information index was 0.3191; a jaccard's genetic similarity matrix and a dendrogram for these cultivars were form ... | 2005 | 16231699 |
| neuroprotective and antioxidant activity of compounds from the aerial parts of dioscorea opposita. | bioactivity-guided fractionation of an ethanol extract of the aerial parts of dioscorea opposita afforded a new compound, 6,7-dihydroxy-2-methoxy-1,4-phenanthrenedione (1), and four known compounds, chrysoeriol 4'-o-beta-d-glucopyranoside (2), chrysoeriol 7-o-beta-d-glucopyranoside (3), alternanthin (4), and daucosterol. the structure of 1 was established on the basis of the interpretation of its 1d and 2d nmr spectrascopic data. compounds 1-4 exhibited both promising neuroprotective effects and ... | 2005 | 16124773 |
| molecular cloning of a genomic dna encoding yam class iv chitinase. | genomic dna for a class iv chitinase was cloned from yam (dioscorea opposita thunb) leaves and sequenced. the deduced amino acid sequence shows 50 to 59% identity to class iv chitinases from other plants. the yam chitinase, however, has an additional sequence of 8 amino acids (a c-terminal extension) following the cysteine that was reported as the last amino acid for other class iv chitinases; this extension is perhaps involved in subcellular localization. a homology model based on the structure ... | 2004 | 15277755 |
| plant chitinase as a possible biocontrol agent for use instead of chemical fungicides. | we investigated whether a plant chitinase can be used as a biocontrol agent instead of chemical fungicides by spraying chitinase e (family 19; class iv) from a yam (dioscorea opposita thunb) alone or together with beta-1,3-glucanase directly onto the surface of a powdery mildew infecting strawberry berries and leaves. results were observed by eye and with a scanning electron microscope. the powdery mildew infecting the strawberries was degraded, mainly by the chitinase, and the disease did not a ... | 2003 | 12619703 |
| [study on the quality of rhizome of dioscorea persimilis]. | to evaluate the quality of rhizome of d. persimilis prain et burk. | 2002 | 12583170 |
| [effect of gradient magnetic field on growth of stem pearls of dioscorea opposita during seedling stage]. | to probe into the bioeffect of gradient magnetic field on the growth and development of medicinal plants. | 2000 | 12512421 |
| [a comparative study on chemical constituents in different processed products of dioscorea opposita]. | the quantitative comparisons of water-soluble extract, alcohol-soluble extract and allantoin in crude and three processed products of dioscorea opposita is carried out by the method recorded in pharmacopoeia and tlcs respectively. as a result, the changes of water-soluble extract and alcohol-soluble extract during the four samples are similar. it is the hightest in the processed product stir-fried with soil and lowest in the crude. but for allantoin, it is the highest in the processed product st ... | 1997 | 12572453 |
| [effect of processing on phospholipid of dioscorea opposita thunb]. | a study of the chemical changes of phospholipid in crude and seven processed samples of dioscorea opposita was carried out by molybdenum blue colorimetry and tlcs. the result shows that the total phospholipid content in the sample processed by honey bran comes out the highest (180.31 mg/100g). | 1993 | 8274210 |
| identification of endogenous gibberellins in dormant bulbils of chinese yam, dioscorea opposita. | it is known that dormancy of the genus dioscorea is induced by application of gibberellin (ga) a(3). to understand the role of gas in dormancy induction, endogenous gas have been identified by kovats retention indices and full mass spectra from capillary gas chromatography-mass spectrometry analysis of purified extract from dormant bulbils of dioscorea opposita thunb. these include ga(4), ga(9), ga(12), ga(19), ga(20), ga(24), ga(36), and ga(53); their presence suggests the occurrence of two bio ... | 1992 | 16653203 |
| [effects of different processed dioscorea opposita thunb. on clearance rate of charcoal particles in mice]. | this paper presents a study on the effects of different processed dioscorea opposita(dpt) on the clearance rate of charcoal particles in mice. the results indicated that all the processed products significantly increased the clearance rate of charcoal particles, but the raw dpt acted best. | 1991 | 1811667 |
| [analysis of nutrient constituents in dioscorea opposita]. | | 1987 | 2964287 |