| development and field evaluation of the sentinel mosquito arbovirus capture kit (smack). | although sentinel animals are used successfully throughout the world to monitor arbovirus activity, ethical considerations and cross-reactions in serological assays highlight the importance of developing viable alternatives. here we outline the development of a passive sentinel mosquito arbovirus capture kit (smack) that allows for the detection of arboviruses on honey-baited nucleic acid preservation cards (flinders technology associates; fta®) and has a similar trap efficacy as standard light ... | 2015 | 26444264 |
| towards management of mosquitoes at homebush bay, sydney, australia. i. seasonal activity and relative abundance of adults of aedes vigilax, culex sitiens, and other salt-marsh species, 1993-94 through 1997-98. | the mosquitoes associated with 2 saline wetlands at homebush bay, sydney, australia, were investigated over 5 consecutive seasons. twenty-one species were collected in adult traps at the 2 sites but the saline wetlands supported larvae of only 4 species: aedes alternans, aedes camptorhynchus, aedes vigilax, and culex sitiens. of these, ae. vigilax and cx. sitiens were the most common, and their peak abundances generally occurred during february and april, respectively. both wetlands were influen ... | 1999 | 10412120 |
| ross river virus in mosquitoes (diptera:culicidae) during the 1994 epidemic around brisbane, australia. | during the summer 1994 outbreak of epidemic polyarthritis in suburban brisbane, 29,931 adult female mosquitoes were collected by octenol-co2 light traps and tested for virus by species in pools of approximately 20 using an in situ enzyme-linked immunoassay. overall, 63 isolations of ross river (rr) virus were made from 7 different mosquito species, including 23 from freshwater-breeding culex annulirostris skuse, 13 from peridomestic aedes notoscriptus (skuse), 4 from aedes procax (skuse), 12 fro ... | 1997 | 9103757 |
| seasonal activity, vector relationships and genetic analysis of mosquito-borne stratford virus. | there are many gaps to be filled in our understanding of mosquito-borne viruses, their relationships with vectors and reservoir hosts, and the environmental drivers of seasonal activity. stratford virus (strv) belongs to the genus flavivirus and has been isolated from mosquitoes and infected humans in australia but little is known of its vector and reservoir host associations. a total of 43 isolates of strv from mosquitoes collected in new south wales between 1995 and 2013 was examined to determ ... | 2017 | 28253306 |
| molecular identification of mosquitoes (diptera: culicidae) in southeastern australia. | dna barcoding is a modern species identification technique that can be used to distinguish morphologically similar species, and is particularly useful when using small amounts of starting material from partial specimens or from immature stages. in order to use dna barcoding in a surveillance program, a database containing mosquito barcode sequences is required. this study obtained cytochrome oxidase i (coi) sequences for 113 morphologically identified specimens, representing 29 species, six trib ... | 2016 | 27217948 |
| foraging ranges of insectivorous bats shift relative to changes in mosquito abundance. | the need to develop effective management strategies for insectivorous bat populations requires an understanding of factors influencing habitat use. availability of pest prey, such as mosquitoes is likely to be one such factor. to assess whether this is the case, we radio-tracked vespadelus vulturnus thomas (little forest bat), a predator of aedes vigilax skuse (saltmarsh mosquito), in saltmarsh and adjacent coastal swamp forest during periods of high and low ae. vigilax abundance. when mosquito ... | 2013 | 23667699 |
| mosquito communities and disease risk influenced by land use change and seasonality in the australian tropics. | anthropogenic land use changes have contributed considerably to the rise of emerging and re-emerging mosquito-borne diseases. these diseases appear to be increasing as a result of the novel juxtapositions of habitats and species that can result in new interchanges of vectors, diseases and hosts. we studied whether the mosquito community structure varied between habitats and seasons and whether known disease vectors displayed habitat preferences in tropical australia. | 2016 | 27388293 |
| Umatilla virus genome sequencing and phylogenetic analysis: identification of stretch lagoon orbivirus as a new member of the Umatilla virus species. | The genus Orbivirus, family Reoviridae, includes 22 species of viruses with genomes composed of ten segments of linear dsRNA that are transmitted between their vertebrate hosts by insects or ticks, or with no identified vectors. Full-genome sequence data are available for representative isolates of the insect borne mammalian orbiviruses (including bluetongue virus), as well as a tick borne avian orbivirus (Great Island virus). However, no sequence data are as yet available for the mosquito borne ... | 2011 | 21897849 |
| arboviruses isolated from mosquitoes collected from urban and peri-urban areas of eastern australia. | to determine the presence of arboviruses in mosquito populations from major urban areas of eastern australia, a total of 67,825 mosquitoes, representing -60 species, was collected and tested from cairns, brisbane, and sydney between january 2005 and april 2008. mosquito pools were screened by inoculation onto mosquito cell cultures and the detection of viral antigen using a panel of flavivirus and alphavirus monoclonal antibodies in an enzyme-linked immunosorbent assay. suspect positive samples ... | 2009 | 19852216 |
| ross river virus transmission, infection, and disease: a cross-disciplinary review. | ross river virus (rrv) is a fascinating, important arbovirus that is endemic and enzootic in australia and papua new guinea and was epidemic in the south pacific in 1979 and 1980. infection with rrv may cause disease in humans, typically presenting as peripheral polyarthralgia or arthritis, sometimes with fever and rash. rrv disease notifications in australia average 5,000 per year. the first well-described outbreak occurred in 1928. during world war ii there were more outbreaks, and the name ep ... | 2001 | 11585790 |
| using next-generation sequencing for dna barcoding: capturing allelic variation in its2. | internal transcribed spacer 2 (its2) is a popular dna barcoding marker; however, in some animal species it is hypervariable and therefore difficult to sequence with traditional methods. with next-generation sequencing (ngs) it is possible to sequence all gene variants despite the presence of single nucleotide polymorphisms (snps), insertions/deletions (indels), homopolymeric regions, and microsatellites. our aim was to compare the performance of sanger sequencing and ngs amplicon sequencing in c ... | 2017 | 27799340 |
| mosquito consumption by insectivorous bats: does size matter? | insectivorous bats have often been touted as biological control for mosquito populations. however, mosquitoes generally represent only a small proportion of bat diet. given the small size of mosquitoes, restrictions imposed on prey detectability by low frequency echolocation, and variable field metabolic rates (fmr), mosquitoes may not be available to or profitable for all bats. this study investigated whether consumption of mosquitoes was influenced by bat size, which is negatively correlated w ... | 2013 | 24130851 |